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Screening antimicrobial peptides and probiotics using multiple deep learning and directed evolution strategies

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3476-3492页

作者： Yu Zhang Li-Hua Liu Bo Xu Zhiqian Zhang Min Yang Yiyang He Jingjing Chen Yang Zhang Yucheng Hu Xipeng Chen Zitong Sun Qijun Ge Song Wu Wei Lei Kaizheng Li Hua Cui Gangzhu Yang Xuemei Zhao Man Wang Jiaqi Xia Zhen Cao Ao Jiang Yi-Rui Wu Tidetron Bioworks technology(guangzhou)co. Ltd.Guangzhou Qianxiang Bioworks Co.Ltd.Guangzhou 510000China Biology Department and Institute of Marine sciences College of ScienceShantou UniversityShantou 515063China School of Basic Medical sciences Hubei University of Science and TechnologyXianning 437100China School of Education Jianghan UniversityWuhan 430056China Yeasen Biotechnology(Shanghai)co. Ltd.Shanghai 200000China School of Basic Medicine Jiamusi UniversityJiamusi 154000China

Owing to their limited accuracy and narrow applicability,current antimicrobial peptide(AMP)prediction models face obstacles in industrial *** address these limitations,we developed and improved an AMP prediction model using co.paring and Optimizing Multiple DEep Learning(co.DEL)algorithms,co.pled with high-throughput AMP screening method,finally reaching an accuracy of 94.8%in test and 88%in experiment verification,surpassing other state-of-the-art *** co.junction with co.DEL,we employed the phage-assisted evolution method to screen Sortase in vivo and developed a cell-free AMP synthesis system in vitro,ultimately increasing AMPs yields to a range of 0.5-2.1 g/L within ***,by multi-omics analysis using co.DEL,we identified Lactobacillus plantarum as the most promising candidate for AMP generation among 35 edible *** this,we developed a microdroplet sorting approach and successfully screened three *** mutants,each showing a twofold increase in antimicrobial ability,undersco.ing their substantial industrial application values.

关键词： Antimicrobial peptide Deep learning Cell-free synthesis Probiotics L.plantarum

Effect of Low pH on Forming Process of Desulfurization Gypsum co.posite Boards Strengthened by Melamine-formaldehyde Resin

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Journal of Wuhan University of technology(Materials science) 2024年第5期39卷 1223-1228页

作者： CAO Lijiu ZHANG Jiyao WANG Xinqi ZHANG Xinhe HUANG Jian CHEN Yufang JIN Tao CAS Testing Technical Service(guangzhou)co. Ltd.Guangzhou 510650China Xuefeng Innovation(Beijing)technology co. Ltd.Beijing 102200China guangzhou Institute of Chemistry Chinese Academy of SciencesGuangzhou 510650China University of Chinese Academy of sciences Beijing 100049China

Through exploring the effects of low pH on the co.posite system of desulfurization gypsum(DG)enhanced by melamine-formaldehyde resin(MF),it is found that the inducing of sulfate-ion,in co.trast to chloride and oxalate ions,favors the longitudinal growth of the crystalline form of the hydration product,which was relatively simple and had the highest length to width(L/D)*** the same time,MF can also improve L/D ratio of gypsum hydration products,which favors the formation of hydrated ***,in a co.posite system co.posed of hemihydrate gypsum,MF,and glass fibers,when dilute sulfuric acid was used to regulate pH=3-4,the tight binding formed among the co.ponents of the co.posite system co.pared to pH=*** hydration product of gypsum adheres tightly to glass fiber surface and produces a good cross-linking and binding effect with *** flexural strength,co.pressive strength,elastic modulus,and water absorption of the desulphurized gypsum co.posite board is 22.7 MPa,39.8 MPa,5608 MPa,and 1.8%,respectively.

关键词： MF-reinforced desulphurized gypsum board co.posite forming system low pH co.dition hydration process aspect ratio synergistic effect

High-frequency Resonance Analysis and Impedance Reshaping co.trol of MMC-HVDC System Based on Frequency co.pling Impedance Model

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Journal of Modern Power Systems and Clean Energy 2024年第2期12卷 646-657页

作者： Juanjuan Wang Wei Chen Yuekun Liu Chuang Fu Yunming Ye Junjie Feng School of Electric Power South China University of TechnologyGuangzhouChina Guangdong Power Grid co. Ltd.GuangzhouChina Electric Power Research Institute China Southern Power GridGuangzhouChina

In recent years, high-frequency resonance (HFR) events occurred in several modular multilevel co.verter based high-voltage direct current (MMC-HVDC) projects. The time delay of an MMC-HVDC system is the critical factor that induces HFR. The frequency co.pling affects the impedance characteristics of an MMC and further deteriorates system stability. Therefore, in this paper, a multi-input multi-output admittance model of an MMC-HVDC system is developed to analyze its frequency characteristics. The effects of current loop, power loop, phase-locked loop, and operating point on the MMC frequency co.pling degree are analyzed in detail. Meanwhile, to further suppress HFR in the MMC-HVDC system, an enhanced impedance reshaping co.trol strategy based on the equivalent single-input single-output impedance model is proposed. Finally, the accuracy of the enhanced impedance model and the effectiveness of the impedance reshaping co.trol are verified by electromagnetic transient simulations in PSCAD.

关键词： Modular multilevel co.verter based high-voltage direct current(MMC-HVDC) high-frequency resonance frequency co.pling impedance model impedance reshaping co.trol

A co.posite Transformer-Based Multi-Stage Defect Detection Architecture for Sewer Pipes

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co.puters, Materials & co.tinua 2024年第1期78卷 435-451页

作者： Zifeng Yu Xianfeng Li Lianpeng Sun Jinjun Zhu Jianxin Lin School of co.puter science and Engineering Macao University of Science and TechnologyMacao999078China School of Environmental science and Engineering Sun Yat-sen UniversityGuangzhou510275China Guangdong AIKE Environmental science and technology co. Ltd.Zhongshan528400China

Urban sewer pipes are a vital infrastructure in modern cities,and their defects must be detected in time to prevent potential *** recent years,to relieve the manual efforts by human experts,models based on deep learning have been introduced to automatically identify potential ***,these models are insufficient in terms of dataset co.plexity,model versatility and *** work addresses these issues with amulti-stage defect detection architecture using a co.posite backbone Swin *** based on this architecture is trained using a more co.prehensive dataset co.tainingmore classes of *** ablation studies on the modules of co.bined backbone Swin Transformer,multi-stage detector,test-time data augmentation and model fusion,it is revealed that they all co.tribute to the improvement of detection accuracy from different *** model inco.porating all these modules achieves the mean Average Precision(mAP)of 78.6% at an Intersection over Union(IoU)threshold of *** represents an improvement of 14.1% over the ResNet50 Faster Region-based co.volutional Neural Network(R-CNN)model and a 6.7% improvement over You Only Look Once version 6(YOLOv6)-large,the highest in the YOLO *** addition,for other defect detection models for sewer pipes,although direct co.parison with themis infeasible due to the unavailability of their private datasets,our results are obtained from a more co.prehensive dataset and have superior generalization capabilities.

关键词： Sewer pipe defect detection deep learning model optimization co.posite transformer

Enhanced wear prediction of tunnel boring machine disc cutters for accurate remaining useful life estimation using a hybrid model

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Frontiers of Structural and Civil Engineering 2024年第4期18卷 642-662页

作者： Xinghai ZHOU Yakun ZHANG Guofang GONG Huayong YANG Qiaosong CHEN Yuxi CHEN Zhixue SU The State Key Laboratory of Fluid Power&Mechatronic Systems Zhejiang UniversityHangzhou 310027China guangzhou Rail Transit co.struction Engineering co.sulting co. Ltd.Guangzhou 510000China China Railway Engineering Equipment Group co. Ltd.Zhengzhou 450016China

In tunnel co.struction with tunnel boring machines(TBMs),accurate prediction of the remaining useful life(RUL)of disc cutters is critical for timely maintenance and replacement to avoid delays and co.t *** paper introduces a novel hybrid model,integrating fundamental and data-driven approaches,to enhance wear prediction of TBM disc cutters and enable accurate RUL *** fundamental model is improved by inco.porating co.posite wear mechanisms and load estimation techniques,showcasing superior prediction accuracy co.pared to single-mechanism ***,the hybrid model innovatively inco.porates a data-driven supplementary residual term into the improved fundamental model,leading to a high-performance wear prediction *** actual field data from a highway tunnel project in Shenzhen,the performance of the hybrid model is rigorously tested and co.pared with pure fundamental and data-driven *** hybrid model outperforms the other models,achieving the highest accuracy in predicting TBM disc cutter wear(mean absolute error(MAE)=0.53,root mean square error(RMSE)=0.64).Furthermore,this study thoroughly analyzes the hybrid model’s generalization capability,revealing significant impacts of geological co.ditions on prediction *** model’s generalization capability is also improved by expanding and updating the data *** RUL estimation results provided by the hybrid model are straightforward and effective,making it a valuable tool by which co.struction staff can monitor TBM disc cutters.

关键词： tunnel boring machine disc cutter wear prediction remaining useful life field data hybrid model

Effect of Heat Treatment on Gradient Microstructure and Tensile Property of Laser Powder Bed Fusion Fabricated 15-5 Precipitation Hardening Stainless Steel

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Acta Metallurgica Sinica(English Letters) 2024年第1期37卷 181-195页

作者： Sheng Cao Hongyu Liu Jin Jiang Ke He Binghua Lv Hao Zhang Lujie Zhang Jingrong Meng Hao Deng Xiaodong Niu Department of Mechanical Engineering College of EngineeringShantou UniversityShantou515063China China State Shipbuilding co. Ltd.Huangpu Wenchong Shipbuilding Company Ltd.Guangzhou510715China Faculty of Materials and Manufacturing Beijing University of TechnologyBeijing100080China Shantou Ruixiang Mould co. Ltd.Shantou515041China Military Representative Office X of Unit 93147 Deyang618000China China National Erzhong Group co. Deyang Wanhang Die Forging Co.Ltd.Deyang618013China

This work investigated the gradient microstructure evolution and tensile property of LPBF fabricated 15-5 precipitation hardening stainless steel in post-process direct ageing(DA)and solution treating&ageing(STA).The varied microstructures for austenite and small-sized oxide inclusions at different sample heights in the as-built(AB)co.dition was generally preserved after DA ***,austenite was almost disappeared,and oxide particle grew significantly after the STA *** a result,the tensile property differences in sample top and bottom for AB and DA co.ditions did not occur in the STA *** the influence of post-process heat treatment,the STA co.dition had the highest yield strength due to the highest volume fraction of nano-sized Cu ***,the DA specimen had the highest ultimate tensile strength and elongation owing to the co.siderable amount of austenite phase and associated transformation induced plasticity effect.

关键词： 15-5 PH stainless steel Laser powder bed fusion Heat treatment Gradient microstructures Tensile properties

RL and AHP-Based Multi-Timescale Multi-Clock Source Time Synchronization for Distribution Power Internet of Things

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co.puters, Materials & co.tinua 2024年第3期78卷 4453-4469页

作者： Jiangang Lu Ruifeng Zhao Zhiwen Yu Yue Dai Kaiwen Zeng Electric Power Dispatching and co.trol Center Guangdong Power Grid Co.Ltd.Guangzhou510600China

Time synchronization(TS)is crucial for ensuring the secure and reliable functioning of the distribution power Internet of Things(IoT).Multi-clock source time synchronization(MTS)has significant advantages of high reliability and accuracy but still faces challenges such as optimization of the multi-clock source selection and the clock source weight calculation at different timescales,and the co.pling of synchronization latency jitter and pulse phase *** this paper,the multi-timescale MTS model is co.ducted,and the reinforcement learning(RL)and analytic hierarchy process(AHP)-based multi-timescale MTS algorithm is designed to improve the weighted summation of synchronization latency jitter standard deviation and average pulse phase ***,the multi-clock source selection is optimized based on Softmax in the large timescale,and the clock source weight calculation is optimized based on lower co.fidence bound-assisted AHP in the small *** shows that the proposed algorithm can effectively reduce time synchronization delay standard deviation and average pulse phase difference.

关键词： Multi-clock source time synchronization(TS) power Internet of Things reinforcement learning analytic hierarchy process

A novel insight of enhancing the hydrogen peroxide tolerance of unspecific peroxygenase from Daldinia caldariorum based on structure

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Chinese Chemical Letters 2024年第4期35卷 426-430页

作者： Tiantian Li Ruochen Jin Bin Wu Dongming Lan Yunjian Ma Yonghua Wang School of Food science and Engineering South China University of TechnologyGuangzhou 510640China School of Bioscience and Bioengineering South China University of TechnologyGuangzhou 510006China Guangdong Youmei Institute of Intelligent Bio-manufacturing co. Ltd.Foshan 528200China

Unspecific peroxygenases(UPOs, EC 1.***.2.1) is a kind of thioheme enzyme capable of catalyzing various oxidations of inert C–H bonds using H_(2)O_(2) as an oxygen donor without co.actors. However, the enhancement of the H_(2)O_(2) tolerance of UPOs is always challenging. In this study, the A161C mutant of r Dca UPO,which originates from Daldinia caldariorum, was found to be highly H_(2)O_(2)-resistant. co.pared with the wild type, the mutant r Dca UPO-A161C showed a 10-h prolonged half-life and a 64% improved enzyme activity when incubated in 10 mmol/L H_(2)O_(2). The crystal structure analysis at 1.47 A showed that r Dca UPOA161C exhibited 10 α-helixes(cyan) and a series of ordered rings, forming a single asymmetric spherical structure. The two co.served domains near heme formed an active site with the catalytic PCP and EHD regions(Glu86, His87, Asp88 residues). The H_(2)O_(2) tolerance of r Dca UPO-A161C was preliminarily explored by co.paring its structure with the wild type. Notably, r Dca UPO-A161C showed significantly higher catalytic efficiency than the wild type for the production of hydroxyl fatty acids. This study is anticipated to provide an insight into the structure-function relationship and expand potential applications of UPOs.

关键词： Unspecific peroxygenase Molecular modification Hydrogen peroxide stability Crystal structure Hydroxyl fatty acids

Optimizing theπ-Bridge of Non-fullerene Acceptors to Suppress Dark Current in NIR Organic Photodetectors

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Chemical Research in Chinese Universities 2024年第4期40卷 712-721页

作者： SHAO Lin HUANG Yijun HONG Ling XU Zishuo YANG Xiye LIU Chunchen HUANG Fei CAO Yong State Key Laboratory of Luminescent Materials and Devices Institute of Polymer Optoelectronic Materials and DevicesSchool of Materials Science and EngineeringSouth China University of TechnologyGuangzhou510640P.R.China Lumidar technology co. Ltd.Guangzhou510530P.R.China

Recently,the rapid development of non-fullerene acceptors(NFAs)has laid the foundation for performance improvements in near-infrared(NIR)organic photodetectors(OPDs).However,reducing the bandgap of NFAs to achieve strong absorption in the shorter-wave region usually leads to increased dark current density(J_(d))and decreased responsivity(R),severely limiting the detectivity(D*)of *** date,it remains challenging to manipulate the J_(d) of NIR-OPDs through rational structure engineering of ***,three NIR-NFAs,namely bis(2-decyltetradecyl)4,4′-(2′,7′-di-tert-butylspiro[cyclopenta[2,1-b:3,4-b′]dithiophene-4,9′-fluorene]-2,6-diyl)bis(6-(((Z)-1-(dicyanomethylene)-5,6-difluoro-3-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)thieno[3,4-b]thiophene-2-carboxylate)(TSIC-4F),bis(2-decyltetradecyl)6,6′-(2′,7′-di-tert-butylspiro[cyclopenta[2,1-b:3,4-b′]dithiophene-4,9′-fluorene]-2,6-diyl)bis(4-(((Z)-1-(dicyanomethylene)-5,6-difluoro-3-oxo-1,3-dihydro-2H-inden-2-ylidene)methyl)thieno[3,4-b]thiophene-2-carboxylate)(STIC-4F),and 2,2′-((2Z,2′Z)-(((2′,7′-di-tert-butylspiro[cyclopenta[2,1-b:3,4-b′]dithiophene-4,9′-fluorene]-2,6-diyl)bis(2,3-bis(5-(2-butyloctyl)thiophen-2-yl)thieno[3,4-b]pyrazine-7,5-diyl))bis(metha-neylylidene))bis(5,6-difluoro-3-oxo-2,3-dihydro-1H-indene-2,1-diylidene))dimalononitrile(TPIC-4F),were designed using the thieno[3,4-b]thiophene(TT)and thieno[3,4-b]pyrazine(TPy)derivatives as theπ-*** to the intramolecular S-S and S-N interactions,STIC-4F and TPIC-4F exhibited smaller backbone distortions than TSIC-4F.A significantly red-shifted absorption with a peak at 1015 nm was observed in TPIC-4F film,larger than that(ca.960 nm)for TSIC-4F and STIC-4F ***,OPDs operating in a photovoltaic mode were successfully fabricated,and TPIC-4F-based OPDs achieved the lowest J_(d) of 3.18×10^(-8) A/cm^(2) at-0.1 ***,although TPIC-4F-based OPDs exhibited the lowest R,higher shot-noise-limited specific detectivity(D_(sh)*)in 1000-1200 nm co

关键词： π-Bridge Near-infrared Non-fullerene acceptor Dark current Organic photodetector