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Oil Crop Science 2022年第4期7卷 180-188页

作者： Junyi Xu Xin Li Jinglian Bai Ying Liu Shaojie Wang Yueting Liu Chunguang Yang technology center of Dalian customs district DalianChina Logistics Management center of Dalian customs district DalianChina

The main advantage of digital PCR(dPCR) is that it facilitates absolute quantification of the target without reference to the standard/calibration *** droplet dPCR has a three-color staining detection function,which enables multiplex PCR *** this study,this technique was used to establish triple dPCR detection for the genetically modified soybean MON87708 × MON89788 with stacked *** absolute quantitative detection was accomplished for the genomic DNA extracted from the homogenized seeds of GM stack MON87708× MON89788 *** results can serve as a reference for the absolute quantitative detection of stacked events of genetically modified crops.

关键词： Absolute quantification Digital PCR Genetically modified soybean MON87708×MON89788 Stacked event

Study on burrowing nematode, Radopholus similis, pathogenicity test system in tobacco as host

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Journal of Integrative Agriculture 2022年第9期21卷 2652-2664页

作者： YANG Si-hua ZHAO Li-rong DING Sha TANG Shi-qiao CHEN Chun ZHANG Huan-xin XU Chun-ling XIE Hui Research center of Nematodes of Plant Quarantine Laboratory of Plant NematologyCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642P.R.China technology center Guangzhou Customs DistrictPeople's Republic of ChinaGuangzhou 510623P.R.China Comprehensive technology and Service center of Huizhou Port customs Huizhou 516080P.R.China

Radopholus similis(Cobb 1893) Thorne(1949) is a destructive migratory endoparasitic plant nematode. In this study,the pathogenic process of R. similis infection in Nicotiana benthamiana(tobacco) was studied using quartz sand culture in laboratory. The results showed that R. similis mainly parasitised the root cortex, leading to cortical cell decomposition and tissue decay. We optimised the inoculation conditions to establish a method for determining the pathogenicity of R. similis as follows:(1) a glass culture tube was filled with quartz sand(about 1/3 of the height) and sterilised twice;(2) 20-day-old N. benthamiana seedlings were transplanted into test tubes and cultivated for 10 days at(25±1)°C;(3) R. similis female nematodes were inoculated in the root rhizosphere at a rate of 150 nematodes per plant;(4) the number of nematodes, disease severity, and growth of the plant at 30 days post-inoculation(dpi) were determined. The pathogenicity of eight R. similis populations from different hosts was determined, which proved the feasibility of this method.

关键词： Radopholus similis Nicotiana benthamiana populations pathogenicity

Risk Analysis of Chemical Hazards Based on the Case of EU Children’s Products Recalled and Study on the Reasonable Limitation of Harmful Element in Toys

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Journal of Biomedical Science and Engineering 2022年第1期15卷 1-5页

作者： Weiqiang Huo Han Fang Xiaoting Chen Shan Zhao Zhiyong Ding Lin Zhe Yang Chen Jin Luo guangzhou customs district technology center Guangzhou China

The trade situation about China’s export of toy products to the EU was introduced. The recall characteristics of China’s toy products by the EU “Safety Gate” in terms of recall frequency, notified countries, taken measures, and reasons for recall were analyzed in this paper, and the risk factors of human health of unqualified products in terms of chemical hazards were studied in this paper. It is found that phthalate, boron, plumbum and cadmium were the main factors. Assessment of risk for boron in toys was conducted. It is suggested to limit the boron element in Slime: the limit of migration of boron in Slime toys for 3- to 6-year-old children shall be less than 704 mg/kg, and the limitation of migration of boron in Slime toys for 7- to 12-year-old children shall be less than 1268 mg/kg.

关键词： Products for Children Chemical Hazard Risk Factors Limitation of Boron

A Facile Strategy for Intrinsic Low-Dk and Low-Df Polyimides Enabled by Spirobifluorene Groups

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Chinese Journal of Polymer Science 2023年第2期41卷 288-296页

作者： Wan-Yi Tan Ling-Feng Jian Wei-Peng Chen Yong-Wen Zhang Xiao-Chuang Lu Wan-Jun Huang Ji-Sheng Zhang Jing-Wu Wu Jun-Li Feng Yi-Dong Liu Ting-Ting Cui Yong-Gang Min Guangdong University of technology(GDUT) Guangzhou510006China Huimai Materials technology(Guangdong)Co. Ltd.Foshan528200China Shenzhen customs Industrial Product Inspection technology center Shenzhen518026China

Modified polyimides(MPIs)show great potential towards 5G communication applications,due to its excellent thermal stability,mechanical property and chemical stability as compared to most of *** fluoride groups or porous structure is favorable to ultra-low dielectric constant(D_(k))and dielectric loss(D_(f)).However,the cost of the fluorinated MPIs is high and their synthetic processes are complicated,and porous MPIs suffer poor mechanical ***,increasing the fraction of free volume is a very effective way to lower D_(k)through introducing more ultra-low-D_(k)air ***,most of this kind of MPIs lag far behind the fluorinated MPIs and the porous MPIs in terms of ultra-low D_(f),hindering the application of MPIs in high-speed communication ***,it is highly desirable to develop intrinsic ultra-low-D_(k)/D_(f)MPIs at high frequency with less fluoric groups and nonporous ***,we introduce a facile and effective strategy to lower D_(k)and D_(f)through introducing rigid and large sterically hindered aromatic groups into *** the one hand,their large steric hindrance effect leads to low D_(k)by increasing intrinsic free *** the other hand,the resulting highly stiff polymer chain and strong intermolecular interaction are favorable to reduce D_(f)by inhibiting dipole *** on this strategy,the spirobifluorene groups are *** as-prepared MPIs show excellent dielectric performance with low D_(k)of 2.74–2.76 and low D_(f)of 0.00599 at 10 GHz,to some extent,exceeding the multiple fluorinated MPI with D_(k)/D_(f)of 2.67/0.00663 at 10 GHz.

关键词： Polyimide High speed communication Low dielectric constant Low dielectric loss

Cutting performance of surgical electrodes by constructing bionic microstriped structures

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Frontiers of Mechanical Engineering 2023年第1期18卷 163-178页

作者： Kaikai LI Longsheng LU Huaping CHEN Guoxiang JIANG Huanwen DING Min YU Yingxi XIE School of Mechanical&Automotive Engineering South China University of TechnologyGuangzhou 510641China Testing and technology center for Industrial Products of Shenzhen customs district Shenzhen 518067China South China University of technology School of Medicine Guangzhou 510006China Department of Orthopaedics Guangzhou First People’s HospitalGuangzhou 510180China Department of General Surgery Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China

Surgical electrodes rely on thermal effect of high-frequency current and are a widely used medical tool for cutting and coagulating biological ***,tissue adhesion on the electrode surface and thermal injury to adjacent tissue are serious problems in surgery that can affect cutting performance.A bionic microstriped structure mimicking a banana leaf was constructed on the electrode via nanosecond laser surface texturing,followed by silanization treatment,to enhance *** effect of initial,simple grid-textured,and bionic electrodes with different wettabilities on tissue adhesion and thermal injury were investigated using horizontal and vertical cutting *** showed that the bionic electrode with high lyophobicity can effectively reduce tissue adhesion mass and thermal injury depth/area compared with the initial *** formation mechanism of adhered tissue was discussed in terms of morphological features,and the potential mechanism for antiadhesion and heat dissipation of the bionic electrode was ***,we evaluated the influence of groove depth on tissue adhesion and thermal injury and then verified the antiadhesion stability of the bionic *** study demonstrates a promising approach for improving the cutting performance of surgical electrodes.

关键词： surgical electrodes tissue adhesion thermal injury bionic structures cutting performance medical tools

Structural characteristics of the SARS-CoV-2 Omicron lineages BA.1 and BA.2 virions

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1381-1383页

作者： Xiaoyu Ma Yanqun Wang Yuanzhu Gao Yiliang Wang An Yan Jiantao Chen Lu Zhang Peiyi Wang Jincun Zhao Zheng Liu Cryo-electron Microscopy center Southern University of Science and TechnologyShenzhenGuangdongChina State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina Health and Quarantine Laboratory Guangzhou Customs District Technology CentreGuangzhouChina guangzhou Laboratory Bio-IslandGuangzhouChina Institute of Infectious disease Guangzhou Eighth People’s Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina

Dear Editor,Variants of concern(VOCs)of SARS-CoV-2 have increased transmissibility,virulence,and *** emergence of new VOCs continues to pose great challenges to public *** detailed structures of the intact Omicron viruses remain *** this study,we report the architecture of both Omicron BA.1 and BA.2 lineages using cryo-electron tomography(cryo-ET).The subtomogram averaging of viral spikes,in both pre-fusion and postfusion conformations are also investigated.

关键词： characteristics Omi resistance.

Research on the Chemical Hazard Risk of Toys Exported to EU from China Based on the Analysis of the EU “Safety Gate” Alerts Cases

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Journal of Biomedical Science and Engineering 2023年第4期16卷 53-64页

作者： Weiqiang Huo Xiaoting Chen Han Fang Haijun Guan Qinglan Li Minyi Huang guangzhou customs technology center Guangzhou China

Based on the analysis of the 2544 alert cases from “Safety Gate” (the EU rapid alert system for dangerous non-food products) of toys exported to the EU market from China in the past five years (2017-2021), this study focuses on the chemical hazards as the key research object, investigates and identifies categories of toy products and related chemicals with high notification rate. The research results have shown that the most seriously and frequently alerted toy products due to chemical hazards from China exported to the EU are plastic dolls, slimes, rubber toy balloons, toy guns and squeezable toys, while the high failure rate of chemicals in sequence are phthalates, boron element, N-Nitrosamines and N-Nitrosatable substances, and bis(2-(dimethylamino)ethyl) ether. This study aims to improve the efficiency of inspection and test of export toys, and provide decision-making references for Chinese toy export enterprises to respond to EU technical trade measures.

关键词： Toy Export Safety Gate Technical Trade Measures

The evolution and functional divergence of 10 Apolipoprotein D-like genes in Nilaparvata lugens

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Insect Science 2024年第1期31卷 91-105页

作者： Jia-Bao Lu Peng-Peng Ren Qiao Li Fang He Zhong-Tian Xu Sai-Nan Wang Jian-Ping Chen Jun-Min Li Chuan-Xi Zhang Citrus Research Institute Southwest University/Chinese Academy of Agricultural SciencesNational Engineering Research Center for CitrusChongqingChina technology center of Wuhan customs district HubeiChina Institute of Insect Science Zhejiang UniversityHangzhouChina

Apolipoprotein D(ApoD),a member of the lipocalin superfamily of proteins,is involved in lipid transport and stress *** only a single copy of the ApoD gene is found in humans and some other vertebrates,there are typically several ApoD-like genes in *** date,there have been relatively few studies that have examined the evolution and functional differentiation of ApoD-like genes in insects,particularly hemi-metabolous *** this study,we identified 10 ApoD-like genes(NlApoD1−10)with distinct spatiotemporal expression patterns in Nilaparvata lugens(BPH),which is an important pest of ***1−10 were found to be distributed on 3 chromosomes in a tandem array of NlApoD1/2,NlApoD3−5,and NlApoD7/8,and show sequence and gene structural divergence in the coding regions,indicating that multiple gene duplication events occurred during *** analysis revealed that NlApoD1−10 can be clustered into 5 clades,with NlApoD3−5 and NlApoD7/8 potentially evolving exclusively in the Delphacidae *** screening using an RNA interference approach revealed that only NlApoD2 was essential for BPH development and survival,whereas NlApoD4/5 are highly expressed in testes,and might play roles in ***,stress response analysis revealed that NlApoD3−5/9,NlApoD3−5,and NlApoD9 were up-regulated after treatment with lipopolysaccharide,H2O2,and ultraviolet-C,respectively,indicating their potential roles in stress resistance.

关键词： Apolipoprotein D BPH evolution functional divergence gene duplication NIApoD

Analysis on the Technical Standardization System of Juvenile Product in Europe and the United States of America and Strategies of Technical Measures of Trade

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World Journal of Engineering and technology 2021年第4期9卷 737-746页

作者： Weiqiang Huo Han Fang Xiaoting Chen Yang Chen Yong Tian Zhigeng Chen guangzhou customs district technology center Guangzhou China Standardization Working Group on Juvenile Products of Standardization Administration of China Beijing China

China is the largest country in the trade of juvenile products in the world. However, the products suffered the loss of technical barriers to trade from Europe and the United States of America increased year by year. This paper analyzes the standard system of juvenile products in Europe and the United States of America with the method of standard comparison, to find its relative technical advantages, explore the reasons for the recall notification of juvenile products exported to the above regions by China, propose strategies for breaking down the technical measures of trade, and promote the development of international juvenile product export trade.

关键词： Technical Standardization System Juvenile Product Technical Measures of Trade

Al-modification for PS-PVD 7YSZ TBCs to improve particle erosion and thermal cycle performances

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Journal of Advanced Ceramics 2022年第7期11卷 1093-1103页

作者： Xiaofeng ZHANG Ming LI Ao ZHANG Shuangquan GUO Jie MAO Chunming DENG Panpan WANG Changguang DENG Junli FENG Min LIU Kesong ZHOU Cheng LAI National Engineering Laboratory for Modern Materials Surface Engineering technology&the Key Lab of Guangdong for Modern Surface Engineering technology Institute of New MaterialsGuangdong Academy of ScienceGuangzhou 510650China PERA guangzhou Information technology Co. Ltd.Guangzhou 510650China Chengdu Holy(Group)Industry Co. Ltd.Chengdou 610000China School of Aviation and Mechanical Engineering Changzhou Institute of TechnologyChangzhou 213032China Shenzhen customs Industrial Products Inspection technology center Shenzhen 518067China

Plasma spray-physical vapor deposition(PS-PVD)as a novel process was used to prepare feather-like columnar thermal barrier coatings(TBCs).This special microstructure shows good strain tolerance and non-line-of-sight(NLOS)deposition,giving great potential application in ***,due to serious service environment of aero-engine,particle erosion performance is a weakness for PS-PVD 7YSZ *** a solution,an Al-modification approach was proposed in this *** in-situ reaction of Al and ZrO2,anα-Al2O3 overlay can be formed on the surface of 7YSZ columnar *** results demonstrate that this approach can improve particle erosion resistance since hardness improvement of Al-modified ***,as another important performance of thermal cycle,it has a better optimization with 350-cycle water-quenching,compared with the as-sprayed TBCs.

关键词： plasma spray-physical vapor deposition(PS-PVD) thermal barrier coatings(TBCs) Al-modification particle erosion resistance thermal cycle performance