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Joint deletion of multifunctional MGF505-7R and H240R genes generates a safe and effective African swine fever virus attenuated live vaccine candidate

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Virologica Sinica 2024年第3期39卷 355-357页

作者： Changjiang Weng Li Huang Guangqiang Ye Division of Fundamental Immunology National African Swine Fever Para-reference LaboratoryState Key Laboratory for Animal Disease Control and PreventionHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China Heilongjiang Provincial key Laboratory of veterinary Immunology Harbin 150069China

African swine fever(ASF)caused by the ASF virus(ASFV)is a highly contagious disease of pigs and wild ***,there are no safe,effective commercial vaccines *** genome of ASFV HLJ/18 contains more than 180 genes,including a few virulence-related *** these key virulence genes through individual deletion and subsequent pathogenicity testing in pigs is a laborious *** addition,some ASFV genes are indispensable for viral replication and cannot be *** the past 30 years,scientists have confirmed that certain ASFV genes particularly those host antiviral innate immune responses,are associated with the virulence of ASFV *** knockout of these genes does not impact viral replication,it does attenuate the virulence of ASFV in *** immunized with these live attenuated vaccine candidates can provide partial or complete *** on these findings,we propose a new concept that ASFV immunomodulatory genes involved in type I interferon(IFN)production,IFN-JAK-STAT signaling,inflammatory responses,cell death(involved in apoptosis,necrosis,and pyroptosis),and autophagy may be related to the pathogenicity of *** unbiased screening may identify more ASFV immunomodulatory or multifunctional genes,simplifying the confirmation of ASFV virulence-related *** line with these theories,we have identified MGF505-7R and H240R as key virulence genes determining the pathogenicity of the ASFV HLJ/18 *** of MGF505-7R,H240R alone or both attenuate the virulence of ASFV HLJ/18 in pigs,providing a paradigm for developing attenuated live vaccines from basic research results.

关键词： attenuated fever swine

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Corrigendum to“3Cpro of FMDV inhibits type II interferon-stimulated JAK-STAT signaling pathway by blocking STAT1 nuclear translocation”[Virol Sin 38(2023)387–397]

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Virologica Sinica 2024年第2期39卷 351-354页

作者： Xiangju Wu Lei Chen Chao Sui Yue Hu Dandan Jiang Fan Yang Laura C.Miller Juntong Li Xiaoyan Cong Nataliia Hrabchenko Changhee Lee Yijun Du Jing Qi Shandong key Laboratory of Animal Disease Control and Breeding Key Laboratory of Livestock and Poultry Multi-omics of MARAInstitute of Animal Science and Veterinary MedicineInstitute of Crop Germplasm ResourcesShandong Academy of Agricultural SciencesJinan250100China College of Life Science Shandong Normal UniversityJinan250358China State key Laboratory of veterinary Etiological Biology National Foot and Mouth Disease Reference LaboratoryKey Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730050China Department of Diagnostic Medicine and Pathobiology College of Veterinary MedicineKansas State UniversityManhattanKS66506USA College of veterinary Medicine and Virus Vaccine research Center Gyeongsang National UniversityJinju52828Republic of Korea

Due to our negligence,the original version of this article,published online on Mar 14,2023,contained some mistakes in several *** Fig.2D,the positions of the bands for protein 3A and 3B were incorrectly *** has been m... 详细信息

关键词： STAT1 FMDV bands

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Effective protective agents against the organ toxicity of T-2 toxin and corresponding detoxification mechanisms: A narrative review

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Animal Nutrition 2024年第1期16卷 251-266页

作者： Pengju Wang Lv-hui Sun Xu Wang Qinghua Wu Aimei Liu Hubei key Laboratory of Diabetes and Angiopathy Medicine Research Institute Xianning Medical College Hubei University of Science and Technology Hubei Hongshan Laboratory College of Animal Science and Technology Huazhong Agricultural University National Reference Laboratory of veterinary Drug Residues (HZAU) and MAO key Laboratory for Detection of veterinary Drug Residues Huazhong Agricultural University College of Life Science Yangtze University

T-2 toxin is one of the most widespread and toxic fungal toxins in food and feed. It can cause gastrointestinal toxicity, hepatotoxicity, immunotoxicity, reproductive toxicity, neurotoxicity, and nephrotoxicity in humans and animals. T-2 toxin is physicochemically stable and does not readily degrade during food and feed processing. Therefore, suppressing T-2 toxin-induced organ toxicity through antidotes is an urgent issue. Protective agents against the organ toxicity of T-2 toxin have been recorded widely in the literature, but these protective agents and their molecular mechanisms of detoxification have not been comprehensively summarized. In this review, we provide an overview of the various protective agents to T-2 toxin and the molecular mechanisms underlying the detoxification effects. Targeting appropriate targets to antagonize T-2 toxin toxicity is also an important option. This review will provide essential guidance and strategies for the better application and development of T-2 toxin antidotes specific for organ toxicity in the future.

关键词： T-2 toxin Organ toxicity Protective agent Detoxification mechanism

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Genome-wide CRISPR screens identify CLC-2 as a drug target for anti-herpesvirus therapy: tackling herpesvirus drug resistance

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Science China Life Sciences 2024年

作者： Fayu Yang Nan Wei Shuo Cai Jing Liu Qingping Lan Hao Zhang Lu Shang Bo Zheng Mi Wang Yingchun Liu Lifang Zhang Chenzhong Fei Wu Tong Changlong Liu Ersheng Kuang Guangzhi Tong Feng Gu Shanghai veterinary research institute Chinese Academy of Agricultural Sciences key Laboratory of veterinary Chemical Drugs and Pharmaceutics Ministry of Agriculture and Rural Affairs State key Laboratory for Animal Disease Control and Prevention College of Veterinary Medicine Lanzhou University Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences institute of Human Virology Zhongshan School of Medicine Sun Yat-Sen University School of Medicine Tsinghua University

The emergence of drug resistance to virus(i.e., acyclovir(ACV) to herpesviruses) has been termed one of the common clinical issues,emphasizing the discovery of new antiviral agents. To address it, a genome-wide clustered regularly interspaced short palindromic repeats(CRISPR) screening was performed in mouse haploid embryonic stem cells infected with pseudorabies virus(PRV), an α-herpesvirus causing human and pig diseases. The results demonstrated that type 2 voltage-gated chloride channels(CLC-2) encoded by one of the identified genes, CLCN2, is a potential drug target for anti-herpesvirus therapy. CLC-2 inhibitors, omeprazole(OME) and 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid(DIDS), can efficiently inhibit infection of multiple herpesviruses in cellulo(i.e., PRV, HSV and EBV), and effectively treat murine herpes simplex encephalitis(HSE). Additionally, DIDS was found to inhibit HSV-1 replication by blocking the PI3K/Akt *** importantly, both DIDS and OME were able to inhibit ACV-resistant HSV-1 strain infection. The study's findings suggest that targeting host-cell factors such as CLC-2 may be a promising approach to tackling herpesvirus drug resistance. The discovery of CLC-2 as a potential drug target for anti-herpesvirus therapy provides a new direction for the development of novel antiviral agents.

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Pangenome and multi-tissue gene atlas provide new insights into the domestication and highland adaptation of yaks

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Journal of Animal Science and biotechnology 2024年第5期15卷 1832-1850页

作者： Daoliang Lan Wei Fu Wenhui Ji Tserang‑Donko Mipam Xianrong Xiong Shi Ying Yan Xiong Peng Sheng Jiangping Ni Lijun Bai Tongling Shan Xiangdong Kong Jian Li Ministry of Education of key Laboratory of Qinghai‑Tibetan Plateau Animal Genetic Resource and Utilization Southwest Minzu UniversityChengduChina College of Animal&veterinary Sciences Southwest Minzu UniversityChengduChina institute of Qinghai‑Tibetan Plateau Southwest Minzu UniversityChengduChina Jiguang Gene biotechnology Co. Ltd.NanjingChina Chengdu Genepre Technology Co. Ltd.ChengduChina Shanghai veterinary research institute Chinese Academy of Agricultural SciencesShanghaiChina

Background The genetic diversity of yak,a key domestic animal on the Qinghai-Tibetan Plateau(QTP),is a vital resource for domestication and breeding *** study presents the first yak pangenome obtained through the de novo assembly of 16 yak *** We discovered 290 Mb of nonreference sequences and 504 new *** pangenome-wide presence and absence variation(PAV)analysis revealed 5,120 PAV-related genes,highlighting a wide range of variety-specific genes and genes with varying frequencies across yak *** component analysis(PCA)based on binary gene PAV data classified yaks into three new groups:wild,domestic,and ***,we pro-posed a‘two-haplotype genomic hybridization model'for understanding the hybridization patterns among breeds by integrating gene frequency,heterozygosity,and gene PAV data.A gene PAV-GWAS identified a novel gene(Bos-Gru3G009179)that may be associated with the multirib trait in Jinchuan ***,an integrated transcrip-tome and pangenome analysis highlighted the significant differences in the expression of core genes and the muta-tional burden of differentially expressed genes between yaks from high and low *** analysis across multiple species revealed that yaks have the most unique differentially expressed m RNAs and lnc RNAs(between high-and low-altitude regions),especially in the heart and lungs,when comparing high-and low-altitude *** The yak pangenome offers a comprehensive resource and new insights for functional genomic studies,supporting future biological research and breeding strategies.

关键词： High-and low-altitude Novel genes Pangenome PAV-GWAS Yak

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Establishment of an indirect immunofluorescence assay for the detection of African swine fever virus antibodies

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Journal of Integrative Agriculture 2024年第1期23卷 228-238页

作者： Wan Wang Zhenjiang Zhang Weldu Tesfagaber Jiwen Zhang Fang Li Encheng Sun Lijie Tang Zhigao Bu Yuanmao Zhu Dongming Zhao State key Laboratory for Animal Disease Control and Prevention National African Swine Fever Para-reference LaboratoryNational High Containment Facilities for Animal Diseases Control and PreventionHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China College of veterinary Medicine Northeast Agricultural UniversityHarbin 150069China

African swine fever(ASF)continues to cause enormous economic loss to the global pig *** there is no safe and effective vaccine,accurate and timely diagnosis of ASF is essential to implement control *** immunofluorescence assay(IFA)is a gold standard serological method recommended by the World Organization for Animal Health(WOAH).In this study,we used primary fetal kidney cells to establish a wild boar cell line(BK2258)that supported the efficient replication of ASF virus(ASFV)SD/DY-I/21 and showed visible cytopathic effect(CPE).Moreover,using BK2258,we established a sensitive and specific IFA for ASFV antibody *** standardize and evaluate the performance of this assay,we used serum samples from pigs infected with the low virulent genotype I SD/DY-I/21 and genotype II HLJ/HRB1/20,and immunized with the vaccine candidate HLJ/18-7GD,field samples,and negative serum *** IFA reacted with the ASFV-positive sera and displayed bright fluorescence *** was no non-specific green fluorescence due to cellular senescence or other cell damage-causing *** to a commercial indirect enzyme-linked immunosorbent assay(iELISA),ASFV antibodies were detected 1–4 days earlier using our *** detection limits of the IFA and iELISA for the same ASFV-antibody positive serum samples were 1:25,600 and 1:6,400,respectively,indicating that the IFA is more sensitive than *** newly established IFA was highly specific and did not cross-react with sera positive for six other important porcine pathogens(i.e.,Classical swine fever virus(CSFV),Porcine reproductive and respiratory syndrome virus(PRRSV),Porcme circovirus type 2(PCV2),Pseudorabies virus(PRV),Foot-and-Mouth disease virus type O(FMDV/O),and Porcine epidemic diarrhea virus(PEDV)).This study thus provides a sensitive,specific,and reliable detection method that is suitable for the serological diagnosis of ASF.

关键词： African swine fever antibody IFA serological method

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Modularized viromimetic polymer nanoparticle vaccines(VPNVaxs) to elicit durable and effective humoral immune responses

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National Science Review 2024年第3期11卷 200-214页

作者： Zichao Huang Xinyu Zhuang Liping Liu Jiayu Zhao Sheng Ma Xinghui Si Zhenyi Zhu Fan Wu Ningyi Jin Mingyao Tian Wantong Song Xuesi Chen key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of Sciences School of Applied Chemistry and Engineering University of Science and Technology of China Changchun veterinary research institute Chinese Academy of Agricultural Sciences Jilin Biomedical Polymers Engineering Laboratory State key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences

Virus-like particle(VLP) vaccines had shown great potential during the COVID-19 pandemic, and was thought to be the next generation of antiviral vaccine technology due to viromimetic structures. However,the time-consuming and complicated processes in establishing a current recombinant-protein–based VLP vaccine has limited its quick launch to the out-bursting pandemic. To simplify and optimize VLP vaccine design, we herein report a kind of viromimetic polymer nanoparticle vaccine(VPNVax), with subunit receptor-binding domain(RBD) proteins conjugated to the surface of polyethylene glycol-b-polylactic acid(PEG-b-PLA) nanoparticles for vaccination against SARS-CoV-2. The preparation of VPNVax based on synthetic polymer particle and chemical post-conjugation makes it possible to rapidly replace the antigens and construct matched vaccines at the emergence of different viruses. Using this modular preparation system, we identified that VPNVax with surface protein coverage of 20%–25% had the best immunostimulatory activity, which could keep high levels of specific antibody titers over 5 months and induce virus neutralizing activity when combined with an aluminum adjuvant. Moreover, the polymer nano-vectors could be armed with more immune-adjuvant functions by loading immunostimulant agents or chemical chirality design. This VPNVax platform provides a novel kind of rapidly producing and efficient vaccine against different variants of SARS-CoV-2 as well as other viral pandemics.

关键词： vaccine polymer nanomaterials SARS-CoV-2

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Evolutionary dynamics and comparative pathogenicity of clade 2.3.4.4b H5 subtype avian influenza viruses,China,2021–2022

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Virologica Sinica 2024年第3期39卷 358-368页

作者： Siru Lin Junhong Chen Ke Li Yang Liu Siyuan Fu Shumin Xie Aimin Zha Aiguo Xin Xinyu Han Yuting Shi Lingyu Xu Ming Liao Weixin Jia National Avian Influenza Para-Reference Laboratory Guangdong Engineering Laboratory for Medicament of Zoonosis Prevention and ControlKey Laboratory of Zoonoses Prevention and Control of Guangdong ProvinceLaboratory for Lingnan Modern AgricultureCollege of Veterinary MedicineSouth China Agricultural UniversityGuangzhou510642China key Laboratory of Zoonoses Key Laboratory of Animal Vaccine DevelopmentMinistry of AgricultureGuangzhou510642China institute of Poultry Management and Diseases Yunnan Animal Science and Veterinary InstituteKunming650000China

The recent concurrent emergence of H5N1,H5N6,and H5N8 avian influenza viruses(AIVs)has led to significant avian mortality *** 2020,frequent human-animal interactions have been *** gain insight into the novel H5 subtype AIVs(i.e.,H5N1,H5N6 and H5N8),we collected 6102 samples from various regions of China between January 2021 and September 2022,and identified 41 H5Nx *** analyses on the evolution and biological properties of these isolates were *** analysis revealed that the 41 H5Nx strains belonged to clade 2.***.4.4b,with 13 related to H5N1,19 to H5N6,and 9 to *** based on global 2.***.4.4b viruses showed that all the viruses described in this study were likely originated from H5N8,exhibiting a heterogeneous evolutionary history between H5N1 and H5N6 during 2015–2022 worldwide.H5N1 showed a higher rate of evolution in 2021–2022 and more sites under positive selection pressure in 2015–*** antigenic profiles of the novel H5N1 and H5N6 exhibited notable *** hemagglutination inhibition assay suggested that some A(H5N1)viruses may be antigenically distinct from the circulating H5N6 and H5N8 *** challenge assays demonstrated that the H5N8 virus(21GD001_H5N8)displayed the highest pathogenicity in mice,followed by the H5N1 virus(B1557_H5N1)and then the H5N6 virus(220086_H5N6),suggesting a heterogeneous virulence profile of H5 AIVs in the mammalian *** on the above results,we speculate that A(H5N1)viruses have a higher risk of emergence in the ***,these findings unveil a new landscape of different evolutionary history and biological characteristics of novel H5 AIVs in clade 2.***.4.4b,contributing to a better understanding of designing more effective strategies for the prevention and control of novel H5 AIVs.

关键词： Avian influenza virus(AIV) H5 subtypes AIVs Evolutionary Pathogenicity

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Characterization of a pangolin SARS-CoV-2-related virus isolate that uses the human ACE2 receptor

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Science China(Life Sciences) 2024年第7期67卷 1502-1513页

作者： Luo-Yuan Xia Xue-Feng Wang Xiao-Ming Cui Yi-Ming Zhang Zhen-Fei Wang En-Tao Li Chang-Fa Fan Ke Song Yuan-Guo Li Run-Ze Ye Fang-Xu Li Dai-Yun Zhu Jie Zhang Zhuang-Zhuang Shi Ming-Zhu Zhang Liang-Jing Li Shi-Jing Shen Song Jin Ya-Wei Zhang Wei-Guang Fu Lin Zhao Wen-Hao Wang Tie-Cheng Wang You-Chun Wang Jia-Fu Jiang Yan-Ling Hu Na Jia Yu-Wei Gao Wu-Chun Cao institute of EcoHealth School of Public HealthCheeloo College of MedicineShandong UniversityJinan 250012China Changchun veterinary research institute Changchun 130122China State key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China Division of Animal Model research Institute for Laboratory Animal ResourcesNational Institutes for Food and Drug ControlBeijing 102629China National institutes for Food and Drug Control Beijing 102629China Life Sciences institute Guangxi Medical UniversityNanning 530020China College of Life Sciences Shandong Normal UniversityJinan 250014China research Unit of Discovery and Tracing of Natural Focus Diseases Chinese Academy of Medical SciencesBeijing 100071China

Various SARS-CoV-2-related coronaviruses have been increasingly identified in pangolins,showing a potential threat to *** we report the infectivity and pathogenicity of the SARS-CoV-2-related virus,PCoV-GX/P2V,which was isolated from a Malayan pangolin(Manis javanica).PCoV-GX/P2V could grow in human hepatoma,colorectal adenocarcinoma cells,and human primary nasal epithelial *** replicated more efficiently in cells expressing human angiotensin-converting enzyme 2(hACE2)as SARS-CoV-2 *** intranasal inoculation to the hACE2-transgenic mice,PCoV-GX/P2V not only replicated in nasal turbinate and lungs,but also caused interstitial pneumonia,characterized by infiltration of mixed inflammatory cells and multifocal alveolar *** population immunity established by SARS-CoV-2 infection and vaccination may not protect people from PCoV-GX/P2V *** findings further verify the hACE2 utility of PCoV-GX/P2V by in vivo experiments using authentic viruses and highlight the importance for intensive surveillance to prevent possible cross-species transmission.

关键词： pangolin SARS-CoV-2-related virus infectivity pathogenicity human cell lines human ACE2 receptor hACE2-transgenic mice

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A rapid and visual detection method for Crimean-Congo hemorrhagic fever virus by targeting S gene

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Journal of Integrative Agriculture 2024年第6期23卷 2149-2153页

作者： Xingqi Liu Zengguo Cao Boyi Li Pei Huang Yujie Bai Jingbo Huang Zanheng Huang Yuanyuan Zhang Yuanyuan Li Haili Zhang Hualei Wang State key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/key Laboratory for Zoonosis research of the Ministry of Education Institute of ZoonosisCollege of Veterinary MedicineJilin UniversityChangchun 130062China key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China

Crimean-Congo hemorrhagic fever(CCHF)is a zoonotic disease caused by the CCHF virus(CCHFV),which is primarily transmitted by ticks(Lorenzo Juanes et al.2023).It is an emerging disease that occurs sporadically in Africa,Asia,and Europe,with a high morbidity and mortality rate,as high as 30%in humans(Ceylan et al.2013).CCHFV,belonging to genus Nairovirus,family Bunyaviridae,was first identified in the Congo in the 1960s.

关键词： hemorrhagic fever

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