检索结果-南通市图书馆

Nematode-induced trap formation regulated by the histone H3K4 methyltransferase AoSET1 in the nematode-trapping fungus Arthrobotrys oligospora

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2023年第11期66卷 2663-2679页

作者： Qiao Miao Zhengqi Wang Ziyu Yin Xiaoying Liu Ran Li Ke-Qin Zhang Juan Li State key laboratory for conservation and utilization of Bio-Resources Key Laboratory for Microbial Resources of the Ministry of EducationSchool of Life SciencesYunnan UniversityKunming 650091China

The methylation of lysine 4 of histone H3(H3K4),catalyzed by the histone methyltransferase KMT2/SET1,has been functionally identified in many pathogenic fungi but remains unexplored in nematode-trapping fungi(NTFs).Here,we report a regulatory mechanism of an H3K4-specific SET1 orthologue,AoSET1,in the typical nematode-trapping fungus Arthrobotrys *** the fungus is induced by the nematode,the expression of AoSET1 is *** of AoSet1 led to the abolishment of ***,the yield of traps and conidia ofΔAoSet1 was significantly lower than that of the WT strain,and the growth rate and pathogenicity were also ***,H3K4 trimethylation was enriched mainly in the promoter of two bZip transcription factor genes(AobZip129 and AobZip350)and ultimately up-regulated the expression level of these two transcription factor *** theΔAoSet1 and AoH3K4A strains,the H3^(K4)me modification level was significantly decreased at the promoter of transcription factor genes AobZip129 and *** results suggest that AoSET1-mediated H3KEme serves as an epigenetic marker of the promoter region of the targeted transcription factor ***,we found that AobZip129 negatively regulates the formation of adhesive networks and the pathogenicity of downstream AoPABP1 and *** findings confirm that the epigenetic regulatory mechanism plays a pivotal role in regulating trap formation and pathogenesis in NTFs,and provide novel insights into the mechanisms of interaction between NTFs and nematodes.

关键词： Arthrobotrys oligospora trap formation epigenetic regulatory histone modification pathogenicity

Prospects of utilization of inter-subspecific heterosis between indica and japonica rice

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2020年第1期19卷 1-10页

作者： ZHANG Gui-quan Guangdong Provincial key laboratory of Plant Molecular Breeding State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/College of AgricultureSouth China Agricultural University

The Asian cultivated rice(Oryza sativa L.) grown worldwide is divided into two subspecies, indica and japonica. It is well known that the heterosis of inter-subspecies is usually stronger than that of intra-subspecies. Since the 1970 s, indica hybrid rice, an intra-subspecific hybrid rice, has being widely used in China and even in the world. However, the inter-subspecific hybrid rice between indica and japonica is still unavailable. The major obstacle is the hybrid sterility of the inter-subspecies. In recent decades, the genetic and molecular basis of indica-japonica hybrid sterility was understood more and more clearly. Some breeding approaches for overcoming inter-subspecific hybrid sterility were proposed and used to develop the indicajaponica hybrid rice. The updated understanding will offer new approaches for development of breeding lines for overcoming indica-japonica hybrid sterility, which facilitates developing of inter-subspecific hybrid rice.

关键词： heterosis reproductive isolation hybrid sterility genetic basis molecular mechanism subspecies hybrid rice

Foci of Future Studies on Abiotic Stress Tolerance of Maize in the Era of Post-Genomics

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2012年第8期11卷 1236-1244页

作者： LI You-zhi FAN Xian-wei LIAO Jiang-xiong State key laboratory for conservation and utilization of Subtropical Agro-Bioresources Ministry of Science and Technology/Key Laboratory of Microbial and Plant Genetic Engineering Ministry of Education/College of Life Science and Technology Guangxi University Guangxi 530005 P.R. China Sugarcane Research Center Chinese Academy of Agriculture Sciences Guangxi 530007 P.R.China

Genetic and metabolic engineering approaches are powerful tools for improving the tolerance of maize to abiotic stresses because they are faster and can afford greater control over agronomically useful traits. However, in-depth understanding of the molecular mechanisms controlling response to abiotic stresses is the prerequisite for successful implementation of these strategies. A great flaw to dissect the biological mechanisms by genome sequencing is that genome sequencing approach could not reflect real-time molecular actions of plants especially under the stresses because the living organisms rarely live in unchanging environments. Post-genomics such as transcriptomics, metabolomics, and proteomics can generate knowledge that is closer to the biological processes. With the development of post-genomics, it can be expected that voluminous data will be generated. This paper proposes that future research on maize stress tolerance in the era of post-genomics should focus on metabolomics and proteomics; stress tolerance of whole plant rather than individual tissues or organs; coordination of expression of genes among tissues; characterization of promoters of stress-responsive genes; interrelation between mechanisms for tolerance to, and growth recovery from the stress; hexose metabolism as well as the glycolysis pathway; and foundation genotypes.

关键词： maize abiotic stresses research foci era of post-genomics

A growth-regulating factor 7(GRF7)-mediated gene regulatory network promotes leaf growth and expansion in sugarcane

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2024年第2期12卷 422-431页

作者： Qiaoyu Wang Yihan Li Dadong Lin Xiaoxi Feng Yongjun Wang Tianyou Wang Hongyan Ding Jisen Zhang State key Lab for conservation and utilization of Subtropical AgroBiological Resources and guangxi key Lab for Saccharum Biology Guangxi UniversityNanning 530005GuangxiChina Center for Genomics and Biotechnology/Fujian Provincial key laboratory of Haixia Applied Plant Systems Biology/key laboratory of Saccharum Biology and Genetic Breeding/National Engineering Research Center for Saccharum Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina

Knowledge of the function of growth-regulating factors(GRFs)in sugarcane(Saccharum officinarum and ***)growth and development could assist breeders in selecting desirable plant ***,limited information about GRFs is available in Saccharum due to their *** this study,22 GRFs were identified in the two species and their conserved domains,gene structures,chromosome location,and synteny were ***7 expression varied among tissues and responded to diurnal ***7-YFP was localized preferentially in the nucleus and appears to act as a transcriptional ***7 positively regulated the size and length of rice leaves,possibly by regulating cell size and plant *** seven potential transcription factors binding to the SsGRF7 promoter in ***,four showed positive expression patterns,and two showed negative expression patterns relative to SsGRF7.

关键词： Expression analysis Growth-regulating factor Leaf development Sugarcane Transcription factors

Experimental Investigation of the Decay from A Ship’s Propeller

在线全文

学校读者我要写书评

暂无评论

China Ocean Engineering 2011年第2期25卷 265-284页

作者： W.Lam G.A.Hamill 宋永臣 D.J.Robinson S.Raghunathan key laboratory of Ocean Energy utilization and Energy conservation of Ministry of Education Dalian University of Technology Queen's University Belfast

In the present study, an experimental investigation of the decay of the maximum velocity and its turbulent characteristics behind a ship propeller, in ＂bollard pull＂ condition （zero speed of advance）, is reported. Velocity measurements were performed in laboratory by use of a Laser Doppler Anemometry （LDA） measurement system. Earlier researchers described that the maximum axial velocity is constant at the initial stage of a ship＇s propeller jet （Fuehrer and Romisch, 1977; Blaauw and van de Kaa, 1978; Berger et al, 1981; Verhey, 1983） as reported in a pure water jet （Albertson et al., 1950; Lee et al., 2002; Dai, 2005）, but a number of researchers disagreed with the constant velocity assumption. The present study found that the maximum axial velocity decays in the zone of flow establishment and the zone of established flow with different rates. The investigation provides an insight into the decays of both the maximum velocity and the maximum turbulent fluctuation in axial, tangential and radial components and the decay of the maximum turbulent kinetic energy. Empirical equations are proposed to allow coastal engineers to estimate the jet characteristics from a ship＇s propeller.

关键词： ship's propeller jet Laser Doppler Anemometry (LDA) maximum velocity decay

Comparison of gene expression in cynomolgus monkeys with preclinical type Ⅱ diabetes induced by different high energy diets

在线全文

学校读者我要写书评

暂无评论

Animal Models and Experimental Medicine 2019年第1期2卷 44-50页

作者： Li-Sha Jin Jun-Hua Rao Li-Biao Zhang Fang Ji Yan-Chun Zhang Xiang-Fen Hao Bai-Lu Peng Xiao-Ming Liu Yun-Xiao Sun Guangdong key laboratory of Animal conservation and Resource utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationGuangdong Institute of Applied Biological Resources South China Botanical Garden

Background : Cynomolgus disease models that are similar to the preclinical stage of human type 2 diabetes mellitus(T2 DM) were established by feeding middle-aged cynomolgus monkeys different high energy diets to study the differential expression of diabetes-related genes. Methods : A total of 36 male monkeys were randomly divided into four groups and fed human diets with high sugar, high fat, double high sugar and fat, and a normal diet. The preclinical diabetes phase was determined by monitoring the metabolic characteristic indices and the results of oral glucose tolerance tests( OGTT). The mRNA expression of 45 diabetes-related genes in peripheral blood leukocytes was analyzed using real-time PCR. Results : A total of 22, 25, and 21 genes were significantly up-regulated( P < 0.05) and 5, 7, and 5 genes were significantly down-regulated( P < 0.05) in the above three induced groups, respectively, compared with the control group. Of the 45 tested genes, the expression profiles of 21 genes were consistent. Most of the expression levels in the double high sugar-and-fat individuals were slightly lower than those in the high glucose and high fat groups, although the expression patterns of the three groups were essentially similar. Conclusion : The different high energy diets all induced diabetes and shared some phenotypic properties with human T2 DM. Most of the expression patterns of the related genes were identical. The gene expression profiles could be used as references for the study of early diagnostic indicators and T2 DM pathogenesis.

关键词： cynomolgus monkey gene expression high energy diet preclinical phase type 2 diabetes

TMK4 receptor kinase negatively modulates ABA signaling by phosphorylating ABI2 and enhancing its activity

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2021年第6期63卷 1161-1178页

作者： Lan Li Bin Li Sirui Zhu Long Wang Limei Song Jia Chen Zhenhua Ming Xuanming Liu Xiushan Li Feng Yu College of Biology State Key Laboratory of Chemo/Biosensing and Chemometrics and Hunan Key Laboratory of Plant Functional Genomics and Developmental RegulationHunan UniversityChangsha 410082China State key laboratory for conservation and utilization of Subtropical Agro‐bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China

In plants, clade A type 2 C protein phosphatases(PP2 CAs) have emerged as major players in abscisic acid(ABA)-regulated stress responses by inhibiting protein kinase activity. However, how different internal and external environmental signals modulate the activity of PP2 CAs are not well known. The transmembrane kinase(TMK) protein4(TMK4), one member of a previously identified receptor kinase subfamily on the plasma membrane that plays vital roles in plant cell growth,directly interacts with PP2 CAs member(ABAInsensitive 2, ABI2). tmk4 mutant is hypersensitive to ABA in both ABA-inhibited seed germination and primary root growth, indicating that TMK4 is a negative regulator in ABA signaling *** analyses indicate that TMK4 phosphorylates ABI2 at three conserved Ser residues,thus enhancing the activity of ABI2. The phosphorylation-mimic ABI2~(S139DS140DS266D)can complement butnon-phosphorylated form ABI2~(S139AS140AS266) Acannot complement ABA hypersensitive phenotype of the loss-of-function mutant abi1-2 abi2-2. This study provides a previously unidentified mechanism for positively regulating ABI2 by a plasma membrane protein kinase.

关键词： ABA responses PP2CA phosphorylation TMK kinases

GenomeSyn:a bioinformatics tool for visualizing genome synteny and structural variations

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2022年第12期49卷 1174-1176页

作者： Zu-Wen Zhou Zhi-Guang Yu Xiao-Ming Huang Jin-Shen Liu Yi-Xiong Guo Ling-Ling Chen Jia-Ming Song State key laboratory for conservation and utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanningGuangxi 530004China College of Stomatology Guangxi Medical UniversityNanningGuangxi 530021China National key laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhanHubei 430070China

Advances in the next-generation and long-read sequencing technologies have promoted the development of genome-wide comparative genomics analysis,and constructing pangenomes and identifying structural variations(SVs)are becoming the frontier of *** genomics is of critical importance for determining the gene function and the evolutionary basis of traits.

关键词： traits Genome structural

Visualization of CO_2 and oil immiscible and miscible flow processes in porous media using NMR micro-imaging

在线全文

学校读者我要写书评

暂无评论

Petroleum Science 2011年第2期8卷 183-193页

作者： Zhao Yuechao SongYongchen Liu Yu Jiang Lanlan Zhu Ningjun key laboratory of Ocean Energy utilization and Energy conservation of the Ministry of Education Dalian University ofTechnology Dalian Liaoning 116024 China

CO2 flooding is considered not only one of the most effective enhanced oil recovery （EOR） methods, but also an important alternative for geological CO2 storage. In this paper, the visualization of CO2 flooding was studied using a 400 MHz NMR micro-imaging system. For gaseous CO2 immiscible displacement, it was found that CO2 channeling or fingering occurred due to the difference of fluid viscosity and density. Thus, the sweep efficiency was small and the final residual oil saturation was 53.1%. For supercritical CO2 miscible displacement, the results showed that piston-like displacement occurred, viscous fingering and the gravity override caused by the low viscosity and density of the gas was effectively restrained, and the velocity of CO2 front was uniform. The sweep efficiency was so high that the final residual oil saturation was 33.9%, which indicated CO2 miscible displacement could enhance oil recovery more than CO2 immiscible displacement. In addition, the average velocity of CO2 front was evaluated through analyzing the oil saturation profile. A special core analysis method has been applied to in-situ oil saturation data to directly evaluate the local Darcy phase velocities and capillary dispersion rate.

关键词： NMR micro-imaging porous media CO2 flooding enhanced oil recovery saturation