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Brain Transcriptome Analysis Reveals Metabolic Changes Adapting to Hyperhaline or Hypohaline Environments in Spotted Scat(Scatophagus argus)

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Journal of Ocean University of China 2024年第3期23卷 755-765页

作者： LIN Ting ZHAI Yi TIAN Changxu JIANG Dongneng SHI Hongjuan JIANG Mouyan LI Guangli DENG Siping Fisheries College Guangdong Ocean UniversityZhanjiang 524088China guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species Zhanjiang 524088China guangdong provincial key laboratory of Pathogenic Biology and Epidemiology for aquatic economic animals Zhanjiang 524088China

The fish brain is crucial for adjusting to environmental *** changes play a vital role in the adaptation to salinity change in aquatic ***,few studies have evaluated the responses of the fish brain to salinity *** evaluate the response to various salinities,spotted scat(Scatophagus argus)was cultured in water with salinity levels of 5(low salinity:LS),25(control group:Ctrl),and 35(high salinity group:HS)for 22 *** brain transcriptome was *** total,1698 differentially expressed genes(DEGs)were identified between the HS and Ctrl groups,and 841 DEGs were identified between the LS and Ctrl *** analysis showed that the DEGs in the HS *** comparison were involved in steroid biosynthesis,terpenoid backbone biosynthesis,fatty acid biosynthesis,ascorbate and aldarate metabolism,other types of O-glycan biosynthesis,and fatty acid *** and dicarboxylate metabolism,one carbon pool by folate,steroid biosynthesis,and cysteine and methionine metabolism were significantly enriched in the LS *** ***,the genes related to metabolism(acc,fas,hmgcr,hmgcs1,mvd,soat1,nsdhl,sqle,cel,fdft1,dnmt3a and mtr)were significantly up-regulated in the HS *** *** genes related to metabolism(lipa,sqle,acc,fas,bhmt,mpst,dnmt3a,mtr,hao2,LOC111225351 and hmgcs1)were significantly up-regulated,while hmgcr and soat1 were significantly down-regulated in the LS *** *** results suggest that salinity stress affects signaling pathways and genes’expressions involved in metabolic processes in the brain,and the differences in metabolism play an important role in adaptation to hyperhaline or hypohaline environments in spotted *** research provides a comprehensive overview of transcriptional changes in the brain under hyperhaline or hypohaline conditions,which is helpful to understand the mechanisms underlying salinity adaptation in euryhaline fishes.

关键词： Scatophagus argus brain salinity transcriptome analysis

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Aquaculture species diversification in China

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Aquaculture and Fisheries 2024年第2期9卷 206-217页

作者： Gen Hua Yue Yi Xuan Tay Joey Wong Yubang Shen Junhong Xia Temasek Life Sciences laboratory National University of Singapore1 Research Link117604Singapore key laboratory of Freshwater aquatic Genetic Resources Ministry of Agriculture and Rural AffairsShanghai Ocean UniversityShanghai201306China State key laboratory of Biocontrol Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic AnimalsCollege of Life SciencesSun Yat-Sen UniversityGuangzhou510275China

China has been the top aquaculture producer in the world since *** 800 species and 240 improved varieties have been used in China’s aquaculture,which is the most diverse collection of species on this *** diversification,along with other aquaculture technologies,contributes significantly to the increase of China’s aquaculture production,whereas the use of too many species in aquaculture has resulted in some issues related to the environment and *** provide an updated overview of the species currently used in Chinese aquaculture in this *** examine critically the main drivers of species diversification in China with *** also introduce Chinese approaches to improve and certify new species and varieties for commercial *** believe that further species diversification is not a priority in China’s aquaculture,while further improvement of aquaculture technologies for major and native species is essential for green,profitable,and sustainable(GPS)*** implications of China’s experiences with species diversification for global aquaculture species diversification are discussed.

关键词： Fish Species Diversification Improvement Future

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Genetic mechanism of body size variation in groupers:Insights from phylotranscriptomics

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Zoological Research 2024年第2期45卷 314-328页

作者： Wei-Wei Zhang Zhuo-Ying Weng Xi Wang Yang Yang Duo Li Le Wang Xiao-Chun Liu Zi-Ning Meng State key laboratory of Biocontrol Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory of Aquatic Economic AnimalsSchool of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510275China Southern laboratory of Ocean Science and Engineering(Zhuhai) ZhuhaiGuangdong 519000China Area of Ecology and Biodiversity School of Biological SciencesUniversity of Hong KongHong Kong SAR 999077China Molecular Population Genetics Group Temasek Life Sciences LaboratorySingapore City 117604Singapore

Animal body size variation is of particular interest in evolutionary biology,but the genetic basis remains largely *** studies have shown the presence of two parallel evolutionary genetic clusters within the fish genus Epinephelus with evident divergence in body size,providing an excellent opportunity to investigate the genetic basis of body size variation in ***,we performed phylotranscriptomic analysis and reconstructed the phylogeny of 13 epinephelids originating from the South China *** genetic clades with an estimated divergence time of approximately 15.4 million years ago were correlated with large and small body size,respectively.A total of 180 rapidly evolving genes and two positively selected genes were identified between the two *** enrichment analyses of these candidate genes revealed distinct enrichment categories between the two *** pathways and genes may play important roles in body size variation in groupers through complex regulatory *** on our results,we speculate that the ancestors of the two divergent groups of groupers may have adapted to different environments through habitat selection,leading to genetic variations in metabolic patterns,organ development,and lifespan,resulting in body size divergence between the two locally adapted *** findings provide important insights into the genetic mechanisms underlying body size variation in groupers and species differentiation.

关键词： Phylotranscriptomics Grouper Body size Rapidly evolving genes(REGs) Positively selected genes(PSGs)

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MiR-106a targets ATG7 to inhibit autophagy and angiogenesis after myocardial infarction

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Animal Models and Experimental Medicine 2024年第4期7卷 408-418页

作者： Guofeng Bai Jinghao Yang Weili Liao Xiaofeng Zhou Yingting He Nian Li Liuhong Zhang Yifei Wang Xiaoli Dong Hao Zhang Jinchun Pan Liangxue Lai Xiaolong Yuan Xilong Wang guangdong provincial key laboratory of laboratory animals Guangdong Laboratory Animals Monitoring InstituteGuangzhouChina guangdong laboratory of Lingnan Modern Agriculture National Engineering Research Center for Breeding Swine IndustryState Key Laboratory of Swine and Poultry Breeding IndustryGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular BreedingCollege of Animal ScienceSouth China Agricultural UniversityGuangzhouGuangdongChina Huidong County Animal Quarantine and Inspection Institute HuizhouGuangdongChina Department of Cardiology Hainan General HospitalHainan Affiliated Hospital of Hainan Medical UniversityHainan Clinical Medicine Research InstitutionHaikouPeople's Republic of China key laboratory of Regenerative Biology Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhouGuangdongChina

Background:Myocardial infarction(MI)is an acute condition in which the heart mus-cle dies due to the lack of blood *** research has suggested that au-tophagy and angiogenesis play vital roles in the prevention of heart failure after MI,and miR-106a is considered to be an important regulatory factor in *** the specific mechanism remains *** this study,using cultured venous endothelial cells and a rat model of MI,we aimed to identify the potential target genes of miR-106a and discover the mechanisms of inhibiting autophagy and ***:We first explored the biological functions of miR-106a on autophagy and angiogenesis on endothelial *** we identified ATG7,which was the down-stream target gene of *** expression of miR-106a and ATG7 was investi-gated in the rat model of ***:We found that miR-106a inhibits the proliferation,cell cycle,autophagy and angiogenesis,but promoted the apoptosis of vein endothelial ***,ATG7 was identified as the target of miR-106a,and ATG7 rescued the inhibition of autophagy and angiogenesis by *** expression of miR-106a in the rat model of MI was decreased but the expression of ATG7 was increased in the infarction ***:Our results indicate that miR-106a may inhibit autophagy and angiogenesis by targeting *** mechanism may be a potential therapeutic treatment for MI.

关键词： angiogenesis ATG7 autophagy miR-106a miRNAs myocardial infarction

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Molecular Cloning and Bioinformatics Analysis of sucC Gene of Vibrio alginolyticus Strain HY9901

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Asian Agricultural Research 2024年第8期16卷 32-37页

作者： Yingzhu WEI Zhiqing WEI Xuelian LIN Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal Disease Control and Healthy Culture&key laboratory of Control for Diseases of aquatic economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To clone the sucC gene of Vibrio alginolyticus strain HY9901 and conduct the bioinformatics analysis.[Methods]Based on the sucC gene of *** strain HY9901,specific primers were designed to amplify the full length sequence by PCR and make further analysis.[Results]The theoretical molecular weight of SucC protein was about 41528.45 Da,and the full length was 1167 bp,encoding 388 amino *** has no signal peptide and transmembrane region,and has a variety of functional *** is predicted that it is mainly located in the cytoplasm,and the ubiquitin and lactate modification sites overlap,and it has high gene homology with Vibrio ***α-helix,random coil and extended strand are the main secondary *** similarity between the constructed three-level structure model and the template is high.[Conclusions]This study reveals the structural characteristics and functional potential of SucC protein,and provides a theoretical basis for the study of drug resistance mechanism and prevention strategies.

关键词： Vibrio alginolyticus Gene amplification sucC gene Succinyl-Coa synthetase Protein post-translational modification Bioinformatics analysis

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Molecular Cloning and Bioinformatics Analysis of msrA Gene from Vibrio alginolyticus Strain HY9901

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Agricultural Biotechnology 2024年第4期13卷 1-5,10页

作者： Zhiqing WEI Zhihang CHEN Yingzhu WEI Na WANG Huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal Disease Control and Healthy Culture&key laboratory of Control for Diseases of aquatic economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]This study was conducted to understand the structure and function of MsrA protein.[Methods]With Vibrio alginolyticus HY9901 as the object of study,primers were designed to amplify the full-length gene of msrA,and its bioinformatics analysis was carried out.[Results]The full length of msrA gene was 639 bp,encoding 212 amino acids,and its theoretical molecular weight was about 23729.60 *** protein had a stable structure,and it was hydrophobic *** structure of signal peptides at the N terminal of the amino acid sequence was predicted,and it was found that there was no signal peptide cleavage site and no transmembrane *** amino acid sequence of MsrA contained multiple signal binding *** subcellular localization showed that MsrA protein was most likely located in the *** analysis showed that MsrA of *** had high homology with other Vibrio species,and the highest homology with *** the prediction of functional domains,MsrA had the function of methionine sulfoxide *** secondary structure prediction,MsrA contained random coils at a proportion of 46.70%,which was the *** similarity between the tertiary structure model of MsrA and template Q87SW6.1.A was 89.15%.PTM analysis showed that MsrA protein had many PTM modification sites such as phosphorylation and glycosylation sites.[Conclusions]This study provides some reference value for further study on the role of MsrA in bacterial antioxidant stress.

关键词： Vibrio alginolyticus Gene cloning MsrA Bioinformatics analysis

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Molecular Cloning of clpX Gene from Vibrio alginolyticus HY9901 and Its Bioinformatics Analysis

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Asian Agricultural Research 2024年第8期16卷 17-22页

作者： Xiaoxin WEN Yuyan HE Jiajie MA Weijie ZHANG Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524088China guangdong provincial key laboratory of aquatic Animal Disease Control and Healthy Culture&key laboratory of Control for Diseases of aquatic economic animals of guangdong Higher Education Institutes Zhanjiang 524088China Chinese Academy of Inspection and Quarantine Beijing 100176China

According to the clpX gene sequence of Vibrio alginolyticus HY9901,a pair of specific primers were designed,and the full length was cloned by PCR and subjected to bioinformatics *** results showed that the clpX gene was 1281 bp in length and encoded 426 amino *** molecular structure formula was C 3842 H 6405 N 1281 O 1598 S 260,with a theoretical protein molecular weight of approximately 1044473.4 kDa and a theoretical pI value of *** clpX gene was predominantly situated within the cytoplasm,exhibiting unstable and hydrophilic protein *** possessed a signal peptide cleavage site,lacked a transmembrane region,and was not associated with any KEGG metabolic ***,it possessed 2 glycine phosphorylation sites,a CAMP-dependent protein kinase phosphorylation site,a C-terminal amidation modification site,6 protein kinase C phosphorylation sites,7 microbody C-terminal target signal sites,and an ATP/GTP *** clpX phylogenetic tree was constructed using the MEGA 5.0 software via the neighbor-joining *** results demonstrated that the clpX of *** exhibited up to 100%affinity with the clpX of Vibrio *** single subunit 3D structure model of the ClpX protein was obtained using the SWISS-MODEL program.A structural and functional analysis of the protein revealed the presence of three distinct ClpX structural and functional *** the prediction of secondary structure,the proportions ofα-helix,random coil,β-sheet and extended strand were 40.38%,37.09%,5.40%and 17.14%,*** analysis of the ClpX protein through the STRING database revealed that the proteins interacting with the ClpX protein were Tig,Atpd,Hflb,Msrb-2,Rpod,Clpp,Clpa,Lon-1,Hfq,and ANP63951.1.A computational analysis of the ClpX protein identified a number of post-translational modification sites,including phosphorylation,acetylation,ubiquitination,glycosylation,methylation,S-palmitoylation,and *** significance of this st

关键词： Vibrio alginolyticus clpX gene Bioinformatics analysis

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A novel mouse model of PEDF-associated serious liver inflammation,hepatic tumorigenesis and cardiovascular injury mimics human nonalcoholic steatohepatitis

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Genes & Diseases 2024年第1期11卷 11-14页

作者： Xinghui Li Haiping Wang Yandi Wu Li Zou Shijie Dengg Xintu Fu Tongsheng Huang Conghui Shen Teng Wu Weibin Cai guangdong Engineering&Technology Research Center for Disease-Model animals Laboratory Animal CenterZhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdong 510080China Department of Biochemistry Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdong 510080China School of Pharmacy Guangdong Pharmaceutical UniversityGuangzhouGuangdong 510006China guangdong provincial key laboratory of Digestive Cancer Research The Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhenGuangdong 518107China

There are no approvedtherapeuticdrugs for nonalcoholic steatohepatitis(NASH)due to the many bottlenecks,including the lack of preclinical animal models that can perfectly mimic human NASH features,such as systemic metabolic disorders,hepatic steatosis,intense liver inflammation and fibrosis,liver tumorigenesis,and cardiovascularcomplications.i,2"Pigment"epitheliumderived factor(PEDF)is a lipid metabolism regulator with high expression in the liver,and its reduced expression in the liver is closely related to the development of nonalcoholic fatty liver disease(NAFLD).

关键词： inflammation PEDF hepatic

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Cloning and Bioinformatics Analysis of hcp Gene in Aeromonas hydrophila

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Asian Agricultural Research 2024年第3期16卷 36-40页

作者： Fan LI Xingjun FU Haiyun FENG Shi WANG Weijie ZHANG Huanying PANG Na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal Disease Control and Healthy Culture&key laboratory of Control for Diseases of aquatic economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To explore the function of hcp gene in Aeromonas hydrophila.[Methods]A pair of specific primers was designed referring to the hcp gene sequence of *** hcp gene was amplified by PCR,and performed bioinformatics analysis.[Results]The hcp gene had a total length of 1650 bp and encoded 549 amino *** theoretical molecular weight of the protein predicted was about 59476.44 *** predicting the N-terminal signal peptide structure of the amino acid sequence,neither obvious signal peptide cleavage site nor signal peptide was found,and the protein had no transmembrane *** amino acid sequence had a N-glycosylation site,4 protein kinase C phosphorylation sites,7 casein kinase II phosphorylation sites,9 N-myristoylation sites,4 isoprene binding sites,10 microbody C-terminal target signal sites,and an ATP/GTP binding site motif A(P-ring).The amino acid sequence of hcp gene of *** was performed homology analysis with other Aeromonas strains,and it showed higher homology with *** the secondary structure,theα-helix,β-sheet,random coil and extended strand accounted for 45.36%,6.01%,37.52%and 11.11%,*** tertiary structure model consisted of 18α-helix and 22β-*** of protein-protein network interaction demonstrated that the proteins interacting with Hcp protein were AHA_3407,nrfA,nirB-1,nirB-2 and AHA_1112.[Conclusions]Through the bioinformatics prediction results,the basic information of hcp gene of *** is preliminarily understood,and the possible function of this protein is predicted,in order to provide guidance for subsequent vaccine research.

关键词： Aeromonas hydrophila Gene amplification hcp gene Bioinformatics analysis

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Molecular Cloning and Bioinformatics Analysis of cyaA Gene of Vibrio alginolyticus Strain HY9901

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Agricultural Biotechnology 2024年第4期13卷 6-10页

作者： Shi WANG Peng ZHOU Yujia ZHANG Weijie ZHANG Na WANG Huanying PANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China guangdong provincial key laboratory of aquatic Animal Disease Control and Healthy Culture&key laboratory of Control for Diseases of aquatic economic animals of guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]This study was conducted to explore the biological functions of cyaA gene of Vibrio alginolyticus.[Methods]With DNA of *** HY 9901 as a template,primers were designed according to the sequence of cyaA gene,and the cyaA gene was amplified by *** analysis was performed.[Results]The cyaA gene of *** HY9901 was 2529 bp in size,and encoded 842 amino *** molecular structure of CyaA protein was C_(4358)H_(6745)N_(1171)O_(1286)S_(35).Its theoretical molecular weight was 97.24167 kDa and the theoretical pI value was *** had no signal peptide and transmembrane *** protein had three N-terminal glycosylation sites,one cAMP and cGMP-dependent protein kinase phosphorylation site,nine protein kinase C phosphorylation sites,nine casein kinase II phosphorylation sites,one tyrosine kinase phosphorylation site,seven N-terminal myristoylation sites,one pentenyl binding site and ten microbody C-terminal localization signal *** localization prediction showed that CyaA protein was mainly located in the nucleus and *** multi-sequence alignment and phylogenetic tree construction,it was concluded that *** had high CyaA homology with other Vibrio *** of *** was clustered with Vibrio fluminensis and Vibrio marinisedimini,and they were closely *** secondary structure of CyaA protein consisted ofα-helixes(43.11%),random coils(38.00%)and extended strands(14.49%).In protein network interaction,it was found that the proteins adjacent to CyaA protein were Crp-2,CpdA,Crr,PtsG-2,ANP67209.1,Crp-1,PykF,Pyk,RelA and Ndk.[Conclusions]This study provides a new idea for formulating strategies for the prevention and control of vibriosis.

关键词： Vibrio alginolyticus cyaA gene Bioinformatics analysis

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