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Genetic Structure and Diversity of Parental Cultivars Involved in China Mainland sugarcane Breeding Programs as Inferred from DNA Microsatellites

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Journal of Integrative Agriculture 2012年第11期11卷 1794-1803页

作者： QI Yong-wen PAN Yong-bao LAO Fang-ye ZHANG Chui-ming FAN Li-na HE Hui-yi LIU Ruin WANG Qin-nan LIU Shao-mou LIU Fu-ye LI Qi-wei DENG Hai-hua guangdong key laboratory of sugarcane improvement and biorefinery Guangzhou Sugarcane Industry Research Institute Guangzhou 510316 P.R.China USDA-ARS Mid-South Area Sugarcane Research Laboratory Houma 70360 USA

To understand genetic structure and diversity of parental cultivars involved in China Mainland sugarcane breeding programs, 92 elite parents and 4 wild relatives were genotyped with 18 microsatellite DNA markers. The genetic similarity （GS） values among the cultivars ranged from 0.346 to 0.960 with an average of 0.533. Among the introduced cultivars, India accessions had the closest genetic distance to China Mainland accessions （0.447）, while Australia accessions have the furthest distance （0.503）. A comparison of allelic diversity among geographical origins showed that there were 22 China Mainland specific alleles, of which 28% were derived from native S. spontaneaum germplasm in China. Model-based genetic structure, clustering, and principal components analyses consistently revealed there were five groups within the 96 accessions. Groups 1, 2, 4, and 5 consisted of all cultivars and group 3 only contained wild germplasm. Group 2 was characterized as the Introduction group with 46 cultivars predominantly introduced from Australia, Taiwan of China, India, and USA. Groups 1, 4, and 5 consisted of cultivars mostly originated from China Mainland, defined as the Complex group, Yacheng lines group, and F134/CP72-1210 group, respectively, upon their pedigree. By understanding the genetic relationships among the parental cultivars, breeders can gain a rational basis for expanding the gene pool and select the best parental accessions for crossing.

关键词： genetic diversity microsatellite DNA marker population structure sugarcane

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Split Nano luciferase complementation for probing protein-protein interactions in plant cells

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Journal of Integrative Plant Biology 2020年第8期62卷 1065-1079页

作者： Feng-Zhu Wang Nannan Zhang Yan-Jun Guo Ben-Qiang Gong Jian-Feng Li guangdong Provincial key laboratory of Plant Resources State Key Laboratory of BiocontrolMOE Key Laboratory of Gene Function and RegulationSchool of Life SciencesSun Yat‐sen UniversityGuangzhou 510275China guangdong Provincial key laboratory of sugarcane improvement and biorefinery Guangdong Bioengineering InstituteGuangzhou 510316China

Deciphering protein-protein interactions(PPIs)is fundamental for understanding signal transduction pathways in *** split firefly luciferase(Fluc)complementation(SLC)assay has been widely used for analyzing ***,concern has risen about the bulky halves of Fluc interfering with the functions of their fusion *** luciferase(Nluc)is the smallest substitute for Fluc with improved stability and ***,we developed a dual-use system enabling the detection of PPIs through the Nluc-based SLC and co-immunoprecipitation *** was realized by coexpression of two proteins under investigation in fusion with the HA-or FLAG-tagged Nluc halves,*** validated the robustness of this system by reproducing multiple previously documented PPIs in protoplasts or Agrobacterium-transformed *** next applied this system to evaluate the homodimerization of Arabidopsis CERK1,a coreceptor of fungal elicitor chitin,and its heterodimerization with other homologs in the absence or presence of ***,split fragments of Nluc were fused to two cytosolic ends of Arabidopsis calcium channels CNGC2 and CNGC4 to help sense the allosteric change induced by the bacterial elicitor ***,these results demonstrate the usefulness of the Nluc-based SLC assay for probing constitutive or inducible PPIs and protein allostery in plant cells.

关键词： system interactions Arabidopsis

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Development Strategy of sugarcane Industry in guangdong Province

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Asian Agricultural Research 2012年第10期4卷 17-22,27页

作者： CHEN Yue-gui WU Jian-tao YANG Jun-xian LI Qi-wei XIE Jing PAN Fang-yin WU Wen-long LIU Fu-ye DENG Hai-hua QI Yong-wen Guangzhou sugarcane Industry Research Institute/guangdong key laboratory of sugarcane improvement & biorefinery

guangdong Province is one of the main producing areas of sugarcane in China, and one of China's three regions with dominant advantage in sugarcane, having good basis and conditions for developing sugarcane industry. In this research, using the SWOT-AHP method, we set 20 assessment indicators (such as the regions with dominant advantage in sugarcane, huge demand for domestic sugar, increased production costs and backward system of sugarcane), to analyze the development strategy of sugarcane industry in guangdong Province, from strengths, weaknesses, opportunities and challenges of sugarcane industrial development in guangdong Province. The results show that in order to promote the development of sugarcane industry in guangdong Province, it is necessary to adopt the SO development strategy (relying on its own strengths and using favorable external environment), to achieve the rapid development.

关键词： guangdong Province sugarcane industry Development

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Rapid Detection of Bt Transgenic sugarcane Using Root Tips by PCR

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Agricultural Biotechnology 2014年第2期3卷 21-24页

作者： Fangye LAO Jianwen CHEN Haihua DENG Yongsheng CHEN Yongwen QI Guangzhou sugarcane Industry Research Institute / guangdong key laboratory of sugarcane improvement and biorefinery

[Objective] This study aimed to establish an effective detection technology for rapidly identifying Bt transgenic sugarcane, promptly removing non-Bt transgenic plants and increasing breeding efficiency of Bt transgenic plants, thereby improving breeding efficiency of insect-resistant sugarcane cultivars in China. [ Method] Approximately 1 mg of root tips and other materials were crushed, placed in the bottom of PCR tubes, added successively with solution I and mineral oil, headed at 95 ℃ for 15 rain, added with solution Ⅱ, and finally added with solution III containing GBt primer for PCR. After separated by using electrophoresis on agarese gel, amplification products were observed and photographed under a gel imaging system. [ Result] Various materials treated with different concentrations of solution led to different amplification results. Specifically, the appropriate concentration of NaOH in solution I was 0.1 -0.2 mol/L; the appropriate pH value of solution II was 2. No bands were amplified from mature leaves, tender leaves and old roots of Bt transgenic sugarcane, while root tips successfully amplified 545 bp target bands. The established method was used to identify and analyze 63 hybrid seedlings of YT 91 -976 × SBR216, results showed that a total of 17 samples exhibited 545 bp bands, accounting for approximately 27% of the total number of hybrid seedlings, indicating that these 17 seedlings were Bt transgenic sugarcane plants. [ Conclusion] The rapid detection method established in this study is conducive to identifying Bt transgenic sugarcane and improving breeding efficiency of insect-resistant sugarcane lines, which provides basis for screening of other transgenic materials.

关键词： Bt transgenie sugarcane Root tips PCR Detection

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AFLP Analysis and Trait Evaluation of Bt Transgenic sugarcane Lines

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Agricultural Biotechnology 2013年第5期2卷 48-51页

作者： Fangye LAO Huiyi HE Yongsheng CHEN Jianwen CHEN Rui LIU Haihua DENG Guangzhou sugarcane Industry Research Institute/guangdong key laboratory of sugarcane improvement and biorefinery

[ Objective] A large number of high-quality Bt transgenic sugarcane lines were obtained using transgenic technologies. This study aimed to explore the genetic background, genetic diversity, important traits and relationship between the heredity and phenotype of these transgenic sugarcane lines, thereby providing basis for further effective utilization and preservation of insect-resistant germplasm resources and improving breeding efficiency of insect-resistant sugarcane in China. [Method] By using six AFLP primers with high polymorphism, the genetic diversity of 13 Bt transgenic sugarcane lines and their donor varieties was analyzed. Growth assay and insect-resistance identification were carried out in a quarantine greenhouse with conventional methods. Evaluation of important economic traits was conducted in the field with conventional methods. [ Result] The polymorphism rate was relatively low. Specifically, the polymorphism rate of the first group was 21.00%, the polymorphism rate of the second group was 31.80%, and the total polymorphism rate was 42.50%. Cluster analysis showed that 13 Bt transgenic sugarcane lines and their donor varieties were divided into two categories, the genetic similarity coefficient between two categories was 0.54. All the six materials in the first group were clustered into category Ⅰ, with genetic similarity of 0. 921 ; nine materials in the second group were clustered into category Ⅱ, with genetic similarity of 0. 898. Bt transgenic sugarcane lines showed abundant phenotypic types and significant trait variations, and the economic traits were generally worse than donor varieties. The insect resistance of 13 Bt transgenic sugarcane lines was divided into five grades. [ Conclusion] The first-generation Bt transgenic sugarcane lines have low genetic diversity, abundant trait variations, high insect resistance and generally poor economic traits, which can only be used as germplasm resources for hybridization. Introducing Bt gene into mult

关键词： Bt transgenic sugarcane Genetic diversity AFLP Trait

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Screening and Simple Identification of Drought-resistant Lines in sugarcane Breeding

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Asian Agricultural Research 2019年第5期11卷 67-70,75页

作者： Fangyin PAN Qingwen LUO Wenlong WU Fuye LIU Jiantao WU Dongyong PENG Yongsheng CHEN Guangzhou sugarcane Industry Research Institute/guangdong key laboratory of sugarcane improvement and biorefinery

Six sugarcane plant lines were selected from seedlings owing to good performance in agronomic characters under drought conditions.A special test with drought stress to death under groove was carried out to select the best drought tolerant clones among them. The proportion of bound water to free water content in leaves,cell cytoplasm membrane permeability in leaves,yield components,etc.,which were regarded as reasonable indexes to identify drought resistance of sugarcane,were measured. The results showed that both YT06-868 and YT05-339 were winners. The two were high-class drought-resistant clones. On some measured indexes,both of them had higher values than ROC22( the main check variety) and on the other ones,they both had the same values as ROC22. The drought resistance of YT05-291 was moderate and between ROC22 and ROC16( the secondary check variety),while the others had poor drought-resistant according to these indexes.

关键词： sugarcane Drought resistance Drought stress Identification

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Effect of 30% Tiange Multifunctional Pesticide-Fertilizer on sugarcane Pests and Yield

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Asian Agricultural Research 2017年第7期9卷 49-52页

作者： Jiangjiang XIE Yuegui CHEN Dongyong PENG Chunqiang YANG Xuejun HUANG Guan WU Cong CHEN Junxian YANG Guangzhou sugarcane Industry Research Institute/guangdong key laboratory of sugarcane improvement and biorefinery

In order to study the effect of 30% Tiange multifunctional pesticide-fertilizer on sugarcane pests and sugarcane yield,the field test was conducted with conventional compound fertilizer and 3% carbofuran fertilizer granules as control. The results showed that 30% Tiange multifunctional pesticide-fertilizer could effectively suppress some pests such as sugarcane borer,thrip,aphid and cockchafer. 90 d after pesticide-fertilizer application,the sugarcane withered heart rate was 1. 55% and 2. 55% in the two test points,respectively,significantly lower than the rate of 15. 40% and 19. 40% under control. Before harvest,the sugarcane borer damage rate in the two test points under pesticide-fertilizer treatment was 8. 70% and 9. 47%,respectively,significantly lower than the rate of 34. 50% and 33. 50% under control,and the sugarcane yield was 21. 16% and 22. 14% higher than under control,respectively. 30% Tiange multifunctional pesticide-fertilizer had nutritional and insecticidal effect,which could help to achieve sugarcane pesticide-fertilizer integration and save labor and costs.

关键词： 30% Tiange Pesticide-fertilizer integration sugarcane pests Control effect Yield

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Enhanced bioethanol production from sugarcane bagasse:combination of liquid hot water and deep eutectic solvent pretreatment for optimized enzymatic saccharification

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Frontiers of Chemical Science and Engineering 2024年第8期18卷 1-13页

作者： Xiaoling Xian Biying Li Shiyong Feng Jiale Huang Xinyuan Fu Ting Wu Xiaoqing Lin School of Chemical Engineering and Light Industry Guangdong Provincial Key Laboratory of Plant Resources BiorefineryGuangdong University of TechnologyGuangzhou 510006China

In the present study,a sustainable pretreatment methodology combining liquid hot water and deep eutectic solvent is proposed for the efficient fractionation of hemicellulose,cellulose,and lignin from sugarcane bagasse,thereby facilitating the comprehensive utilization of both C5 and C6 *** application of this combined pretreatment strategy to sugarcane bagasse led to notable enhancements in enzymatic saccharification and subsequent *** results demonstrate that liquid hot water-deep eutectic solvent pretreatment yielded 85.05±0.66 g·L^(-1)of total fermentable sugar(glucose:60.96±0.21 g·L^(-1),xylose:24.09±0.87 g·L^(-1))through enzymatic saccharification of sugarcane ***,fermentation of the pretreated sugarcane bagasse hydrolysate yielded 34.33±3.15 g·L^(-1)of *** findings confirm the effectiveness of liquid hot water-deep eutectic solvent pretreatment in separating lignocellulosic components,thus presenting a sustainable and promising pretreatment method for maximizing the valuable utilization of biomass resources.

关键词： sugar cane bagasse synergistic pretreatment enzymatic saccharification ethanol

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Application of AHP and DTOPSIS in sugarcane Breeding

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Agricultural Biotechnology 2013年第5期2卷 9-12,16页

作者： Jiantao WU Junxian YANG Fuye LIU Qiwei LI Wenlong WU Fangyin PAN Jing XIE Yuegui CHEN Guangzhou sugarcane Industry Research Institute/guangdong key Lab of sugarcane improvement & biorefinery

The weights of ten important traits of sugarcane, including the number of hair groups, plant height, defoliation capability, stalk diameter, ratooning property, stalk number of disease resistance, cane yield, sucrose content and sugar yield, were distributed with the Analytic Hierarchy Process （AHP） method. Ten Yuetang series of sugarcane lines were evaluated by Dynamic Technique for Order Preference by Similarity to Ideal Solution （DTOPSIS）. AHP results indicated that the weights of ten traits were 0. 013 4, 0.022 7, O. 030 2, 0.040 8, 0.065 3, 0.088 1, 0.116 2, 0. 160 6, 0.198 4 and 0.264 5, respectively, with random consistency ratio C.R. =0.098 1 〈0.1, suggesting that the distribution of the weights of evaluation indicators was reasonable. Comprehensive evaluation of 10 sugarcane lines by DTOPSLS method combining with the conclusion of AHP showed that the comprehensive performances of Yuetang 03-393 and Yuetang 03-373 were much better than other sugarcane lines, with relative approach degree Ci ranking the first and second, respectively, which was basically consistent with the practical survey, suggesting that Yuetang 03-393 and Yuetang 03-373 were suitable for large-scale popularization. The above results confirmed the feasibility of applying AHP and DTOPSIS in comprehensive evaluation of new crop varieties and provided reference for the breeding of new sugarcane lines.

关键词： AHP DTOPSIS sugarcane Breeding Comprehensive evaluation

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Advances in Studies of Genetic improvement of sugarcane

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Asian Agricultural Research 2016年第11期8卷 66-70页

作者： Mingfu WEN Junxian YANG Fangyin PAN Wenlong WU Yuegui CHEN Jinyan GUAN Guangzhou sugarcane Industry Research Institute / guangdong key Lab of sugarcane improvement & biorefinery

sugarcane(Saccharum spp.) is a large perennial herbaceous plant that is cultivated in tropical and subtropical regions of the world,and it is also one of the most efficient crops in the world in converting energy from sunlight into chemical energy. As an essential sugar crop and energy crop,sugarcane is receiving an increasing concern for its variety improvement. Traditional breeding and cultivation techniques have contributed a lot to increasing sugarcane yield and sucrose content. In recent years,development and application of biotechnology provide much help for genetic improvement of sugarcane. For convenience of breeders fully knowing advances in studies of sugarcane genetic improvement,this paper elaborated conventional breeding,genomics,GM technology,and molecular marker assisted breeding.

关键词： sugarcane Genetic improvement Genomics Transgene technology Molecular marker assisted breeding

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