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BmMD-2A responds to 20-hydroxyecdysone and regulates Bombyx mori silkworm innate immunity in larva-to-pupa metamorphosis

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insect Science 2023年第2期30卷 411-424页

作者： Ruonan Zhang Xue Chen Yuan Wang Xu Bai Qiong Yang Yangjin Zhong Xiao-Qiang Yu Fengliang Jin Wanying Yang guangdong laboratory for Lingnan Modern Agriculture Laboratory of Insect Molecular Biology and BiotechnologyGuangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular BreedingCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou510642China guangdong laboratory of Bio-Pesticide Creation and Application of guangdong Province College of Plant ProtectionSouth China Agricultural UniversityGuangzhou510642China The Sericultural and Agri-Food Research Institute of the guangdong Academy of Agricultural Sciences GuangzhouChina guangdong Provincial key laboratory of insect developmental biology and applied technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

20E-hydroxyecdysone(20E)plays important roles in larval molting and metamorphosis in insects and is also involved in the insect innate immune *** metamorphosis is a highly successful strategy for environmental adaptation and is the most vulnerable stage during which the insect is susceptible to various pathogens.20E regulates a series of antimicrobial peptides(AMPs)through the immunodeficiency(IMD)pathway activation in Drosophila;nevertheless,whether other immune pathways are involved in 20E-regulated insect immunity is *** previous studies showed that BmMD-2A is a member of the MD-2-related lipid recognition(ML)family of proteins that are involved in the Bombyx mori innate immunity Toll signaling *** this study,we further demonstrate that BmMD-2A is also positively regulated by 20E,and the BmMD-2A neutralization experiment suggested that 20E activates some downstream immune effect factors,the AMP genes against Escherichia coli and Staphylococcus aureus,through the regulation of BmMD-2A in larval metamorphosis,implying that *** may use the Toll-ML signaling pathway to maintain innate immune balance in the larval-pupal metamorphosis stage,which is a different innate immunity pathway regulated by 20E compared to the IMD pathway in Drosophila.

关键词： AMPs BmMD-2A 20E innate immune metamorphosis

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Egg tanning improves the efficiency of CRISPR/Cas9-mediated mutant locust production by enhancing defense ability after microinjection

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Journal of Integrative Agriculture 2021年第10期20卷 2716-2726页

作者： ZHANG Ting-ting WEN Ting-mei YUE Yang YAN Qiang DU Er-xia FAN San-hong Siegfried ROTH LI Sheng ZHANG Jian-zhen ZHANG Xue-yao ZHANG Min Research Institute of applied biology Shanxi UniversityTaiyuan 030006P.R.China School of Life Science Shanxi UniversityTaiyuan 030006P.R.China State key laboratory of Cotton biology/key laboratory of Plant Stress biology School of Life SciencesHenan UniversityKaifeng 475004P.R.China guangdong Provincial key laboratory of insect developmental biology and applied technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou 510631P.R.China Institute for developmental biology University of CologneCologne 50674Germany

The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant *** the construction of CRISPR/Cas9-mediated dsLmRNase2^(–/–)mutant locusts,we found that the tanned eggs which experienced a 20-min contact with the oocyst exhibited a higher success rate compared to fresh newly-laid eggs that were less ***,the heritable efficiency of the dsLmRNase2 deletion to the next generation G_(1 )progeny was similar between adults derived from the tanned or less tanned engineered ***,the similar effective mutant ratios in the normally developed eggs and G_(0) adults of tanned and less tanned eggs also indicated that tanning did not reduce the absolute mutation efficiency induced by CRISPR/***,we found that the syncytial division period,which was longer than the time for tanning,conferred a window period for microinjection treatment with efficient mutation in both tanned and less tanned *** further found that tanned eggs exhibited a higher hatching rate due to a reduced infection rate following *** the anti-pressure and ultrastructure analyses indicated that the tanned eggs contained compressed eggshells to withstand increased external *** summary,tanned eggs possess stronger defense responses and higher efficiency of genome editing,providing an improved model for developing Cas9-mediated gene editing procedures in locusts.

关键词： brown tanned eggs CRISPR/Cas9 mutant locusts microinjection defense ability

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Identification and functional characterization of doublesex gene in the testis of Spodoptera litura

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insect Science 2019年第6期26卷 1000-1010页

作者： Qian Du Liang Wen Si-Chun Zheng Hong-Lun Bi Yong-Ping Huang Qi-Li Feng Lin Liu Guangzhou key laboratory of insect Development Regulation and applied Research Institute of Insect Science and Technology School of Life Sciences South China Normal University GuangzhouChina key laboratory of insect developmental and Evolutionary biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science Chinese Academy of Sciences ShanghaiChina

Fusion of the testis occurs in most Lepidoptera insects, including Spodoptera litura, an important polyphagous pest. Testicular fusion in S. litura is advantageous for male reproduction, and the molecular mechanism of fusion remains unknown. Doublesex influences the formation of genitalia, the behavior of courtship, and sexually dimorphic traits in fruit-fly and silkworm, and is essential for sexual differentiation. However, its purpose in the testis of S. litura remains unknown. The doublesex gene of S. litura (Sldsx) has male-specific SldsxM and female-specific SldsF isoforms, and exhibits a higher expression level in the male testis. At the testicular fusion stage (L6D6), Sldsx attained the highest expression compared to the pre-fusion and post-fusion periods. Moreover, Sldsx had a higher expression in the peritoneal sheaths of testis than that of germ cells in the follicle. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9) was applied to S. litura to determine the role of Sldsx. A mixture of single guide RNA messenger RNA and Cas9 protein (300 ng/μL each) was injected into eggs within 2 h following oviposition. CRISPR/Cas9 successfully induced genomic mutagenesis of Sldsx at Go generation. The mutant males had smaller testis surrounded by less tracheae. Moreover, the mutant males had abnormal external genitalia and could not finish mating with wild-type females. Additionally, testes were fused for almost all mutant males. The results showed that Sldsx was not related to testicular fusion, and is required for both testis development and the formation and function of external genitalia in S. litura. The main roles of doublesex on the male are similar to other insects.

关键词： CRISPR/Cas9 system doublesex external genitalia Spodoptera litura testis

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Comprehensive silk gland multi-omics comparison illuminates two alternative mechanisms in silkworm heterosis

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Zoological Research 2022年第4期43卷 585-596页

作者： Han Xu Lei Chen Xiao-Ling Tong Hai Hu Li-Yuan Liu Gui-Chun Liu Ya-Nan Zhu Ruo-Ping Zhao Wen Wang Fang-Yin Dai Xin Li Hui Xiang School of Medicine Shenzhen Campus of Sun Yat-Sen UniversityShenzhenGuangdong 518107China guangdong Provincial key laboratory of insect developmental biology and applied technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouGuangdong 510631China School of Ecology and Environment Northwestern Polytechnical UniversityXi’anShaanxi 710072China State key laboratory of Silkworm Genome biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest UniversityChongqing 400715China State key laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China

Heterosis is a common phenomenon in plants and animals with diverse underlying ***,we applied two widely used silkworm hybrid systems and performed multi-omics analysis to identify possible intrinsic associations between different hybrid strategies and epigenetic mechanisms with silkworm *** found significant differences in the silk gland transcriptomic landscape between the two systems,including differentially expressed genes and expression patterns in the hybrid offspring compared to their *** the quaternary hybrid system,hybrid vigor was primarily due to upregulated genes and the parent-dominant upregulated expression pattern,involving multiple transport processes,cellular nitrogen compound catabolism,glucose metabolism,and tricarboxylic acid *** the binary system,hybrid vigor was mainly due to the down-regulated genes and transgressively down-regulated expression pattern,mainly involving basic nitrogen synthesis metabolism and body *** also demonstrated that DNA methylation may affect hybrid vigor by regulating the expression of several heterosis-related ***,this study revealed two alternative mechanisms that may contribute to silkworm heterosis,both of which facilitate the efficient utilization of energy and nitrogen for silk production.

关键词： Heterosis Silkworm Hybrid systems Multi-omics Silk production

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Clathrin-dependent endocytosis predominantly mediates protein absorption by fat body from the hemolymph in Bombyx mori

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insect Science 2020年第4期27卷 675-686页

作者： Yu-Jie Wang Shu-Yan Li Jia-Ye Zhao Kang Li Jing Xu Xian-Ying Xu Wen-Mei Wu Rong Yang Yang Xiao Ming-Qiang Ye Ji-Ping Liu Yang-Jin Zhong Yang Cao Hui-Yu Yi Ling Tian guangdong Provincial key Lab of Agro-Animal Genomics and Molecular Breeding/guangdong Provincial Sericulture and Mulberry Engineering Research Center College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina guangdong Provincial key laboratory of insect developmental biology and applied technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina The Sericultural and Agri-Food Research Institute of the guangdong Academy of Agricultural Sciences GuangzhouChina

During insect larval-pupal metamorphosis,proteins in the hemolymph are absorbed by the fat body for the maintenance of intracellular homeostasis;however,the type of proteins and how these proteins are internalized into the fat body are *** Bombyx mori,the developmental profiles o f total proteins in the hemolymph and fat body showed that hemolymph-decreased protein bands(55-100 kDa)were in accordance with those protein bands that increased in the fat *** o f clathrin-dependent endocytosis predominantly blocked the transportation o f 55-100 kDa proteins from the hemolymph into the fat body,which was further verified by RNA interference treatment o f *** hexamerins were shown to comprise〜90%of the total identified proteins in both the hemolymph and fat body by mass spectrum(MS)*** addition,hemolymphspecific proteins were mainly involved in material transportation,while fat body-specific proteins particularly participated in *** this paper,four hexamerins were found for the first time,and potential proteins absorbed by the fat body from the hemolymph through clathrin-dependent endocytosis were *** study sheds light on the protein absorption mechanism during insect metamorphosis.

关键词： Bombyx mori clathrin-mediated endocytosis fat body hemolymph uptake of proteins

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Hox-determined appendage regeneration restores cockroach courtship rituals

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Science China Life Sciences 2024年

作者： Xingmei Mai Mingtao Liao Fangming Zhao Ethan Yihao Li Fangzheng Liu Renaguli Aisa Sichen Wu Nan Chen Sheng Li Chonghua Ren guangdong Provincial key laboratory of insect developmental biology and applied technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal University Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal University Xinyang Aviation Vocational College High School Affiliated to South China Normal University

Dear Editor,The German cockroach,Blattella germanica (insecta,Blattodea),is one of the most synanthropic cosmopolitan pests affecting public health,which is often described as“little mighty”because of its extreme vitality and *** characterization in cockroaches is primarily due to two crucial biological traits,strong regenerative capacity and excellent fecundity(Ren et al.,2023),but nothing is known about the underlying relationship between these two traits in terms of physiology and *** have evolved diverse appendages (***,labial palp,maxillary palp,leg,wing,and cercus) that are used for locomotion,feeding,communication,and other vital functions (Chen et al.,2022;Li et al.,2024).As a crucial adaptive strategy,appendage regeneration enables animals to restore injured body parts and their associated functions (Tanaka,2016),significantly enhancing survival ability and ecological adaptation (Zhong et al.,2023).

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An Unprecedented Efficiency with Approaching 21%Enabled by Additive‑Assisted Layer‑by‑Layer Processing in Organic Solar Cells

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Nano-Micro Letters 2025年第2期17卷 372-375页

作者： Shuai Xu Youdi Zhang Yanna Sun Pei Cheng Zhaoyang Yao Ning Li Long Ye Lijian Zuo Ke Gao key laboratory of Advanced Green Functional Materials College of ChemistryChangchun Normal UniversityChangchun 130032People’s Republic of China Shandong Provincial key laboratory for Science of Material Creation and Energy Conversion School of Chemistry and Chemical EngineeringShandong UniversityQingdao 266237People’s Republic of China College of Polymer Science and Engineering Sichuan UniversityChengdu 610065People’s Republic of China State key laboratory and Institute of Elemento‑Organic Chemistry Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer MaterialsRenewable Energy Conversion and Storage Center(RECAST)College of ChemistryNankai UniversityTianjin 300071People’s Republic of China Institute of Polymer Optoelectronic Materials and Devices State key laboratory of Luminescent Materials and Devices South China University of TechnologyGuangzhou 510640People’s Republic of China Tianjin key laboratory of Molecular Optoelectronic Sciences School of Materials Science and EngineeringTianjin UniversityTianjin 300350People’s Republic of China State key laboratory of Silicon Materials MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationDepartment of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China

Recently published in Joule,Feng Liu and colleagues from Shanghai Jiaotong University reported a record-breaking 20.8%power conversion efficiency in organic solar cells(OSCs)with an interpenetrating fibril network active layer morphology,featuring a bulk p-in structure and proper vertical segregation achieved through additive-assisted layer-by-layer *** optimized hierarchical gradient fibrillar morphology and optical management synergistically facilitates exciton diffusion,reduces recombination losses,and enhances light capture *** approach not only offers a solution to achieving high-efficiency devices but also demonstrates the potential for commercial applications of OSCs.

关键词： Organic solar cells Additive-assisted layer-by-layer processing Three-dimensional fibril morphology Bulk p-i-n structure Optical management

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Surface-rare-earth-rich upconversion nanoparticles induced by heterovalent cation exchange with superior loading capacity

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Journal of Materials Science & technology 2022年第2期97卷 223-228页

作者： Meifeng Wang Yiru Qin Wei Shao ZhiWang Cai Xiaoyu Zhao Yongjun Hu Tao Zhang Sheng Li Mark T.Swihart Yang Liu Wei Wei MOE&guangdong Provincial key laboratory of Laser Life Science Guangzhou Key Laboratory of Spectral Analysis and Functional ProbesCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Department of Chemical and Biological Engineering University at Buffalothe State University of New YorkBuffaloNY 14260United States guangdong Provincial key laboratory of insect developmental biology and applied technology Institute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhou 510631China State key laboratory Breeding Base of Green Chemistry Synthesis technology and College of Chemical Engineering Zhejiang University of TechnologyHangzhou 310014China

Surface modification of different functional molecules onto NaREF_(4)(RE=rare earth)upconversion nanoparticles(UCNPs)impart their multiple *** molecules can be loaded onto NaREF_(4) UCNPs through the formation of coordination bonds between the surface-exposed RE^(3+) ions and the appropriate chemical groups of functional *** density of surface RE^(3+) ions directly determines the loading efficiency of Na REF4 ***,NaREF_(4) is a binary cation system,rendering the surface-distributed Na;and RE^(3+) ions remains a ***,we develop an effective strategy to significantly enhance the density of surface RE^(3+) ions,thus maximizing the loading capacity of NaREF_(4) *** strategy is based on a heterovalent cation exchange(HCE)reaction in the surface region in which Na^(+)ions are replaced by RE^(3+) *** density of surface ligands enhances from 3.6 to 8.8 molecules/nm^(2) after reaction,suggesting that the loading efficiency increases by approximately 150%.Benefiting from the improved loading capacity,we demonstrate such surface-RE-rich nanoparticles have the ability to offer higher colloidal stability and more desirable photodynamic therapy(PDT)*** work not only advances our understanding of cation exchange reactions in RE-based nanoparticles,but also provides significant value for considerable applications such as sensing,bioimaging,and therapy.

关键词： Heterovalent cation exchange Surface-rare-earth-rich Upconversion nanoparticles Superior loading capacity

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Identification of LmUAP1 as a 20-hydroxyecdysone response gene in the chitin biosynthesis pathway from the migratory locust, Locusta migratoria

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insect Science 2018年第2期25卷 211-221页

作者： Xiao-Jian Liu Ya-Wen Sun Da-Qi Li Sheng Li En-Bo Ma[] Jian-Zhen Zhang Research Institute of applied biology College of Life Science Shanxi University Taiyuan China key laboratory of insect developmental and Evolutionary biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

In Locusta migratoria, we found that two chitin biosynthesis genes, UDP N-acetylglucosamine pyrophosphorylase gene LmUAP1 and chitin synthase gene Lm- CHS1, are expressed mainly in the integument and are responsible for cuticle formation. However, whether these genes are regulated by 20-hydroxyecdysone （20E） is still largely unclear. Here, we showed the developmental expression pattern ofLmUAP1, LmCHS1 and the corresponding 20E titer during the last instar nymph stage of locust. RNA interference （RNAi） directed toward a common region of the two isoforms of LmEcR （LmEcRcom） reduced the expression level of LmUAP1, while there was no difference in the expression of LmCHS1. Meantime, injection of 20E in vivo induced the expression of LmUAP1 but not LmCHS1. Further, we found injection-based RNAi of LmEcRcom resulted in 100% mortality. The locusts failed to molt with no apolysis, and maintained in the nymph stage until death. In conclusion, our preliminary results indicated that LmUAP1 in the chitin biosynthesis pathway is a 20E late-response gene and LmEcR plays an essential role in locust growth and development, which could be a good potential target for RNAi-based pest control.

关键词： chitin synthesis Locusta migratoria regulation RNA interference 20-hydroxyecdysone

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Discovering genes responsible for silk synthesis in Bombyx moriby piggyBac-based random insertional mutagenesis

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insect Science 2019年第5期26卷 821-830页

作者： Xing-Bao Feng Zi-Wen Zheng Xian Zhang Jun Gu Qi-Li Feng Li-Hua Huang Guangzhou key laboratory of insect Development Regulation and applied Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

Silkworm mutants are valuable resources for both transgenic breeding and gene discovery. PiggyBac-based random insertional mutagenesis has been widely used in gene functional studies. In order to discover genes involved in silk synthesis, a piggyBac-based random insertional library was constructed using Bombyx mori, and the mutants with abnormal cocoon were particularly screened. By this means, a “thin cocoon” mutant was identified. This mutant revealed thinner cocoon shell and shorter posterior silk gland (PSG) compared with the wild type. The messenger RNA (mRNA) levels of all the three fibroin genes, including Fib-H, Fib-L and P25, were significantly down-regulated in the PSG of mutants. Four piggyBac insertion sites were identified in Aquaporin (AQP), Longitudinals lacking protein-like {Lola), Glutamyl aminopeptidase-like (GluAP) and Loc101744460. The mRNA levels of all the four genes were significantly altered in the silk gland of mutants. In particular, the mRNA amount of AQP, a gene responsible for the regulation of osmotic pressure, decreased dramatically immediately prior to the spinning stage in the anterior silk gland of mutants. The identification of the genes disrupted in the “thin cocoon” mutant in this study provided useful information for understanding silk production and transgenic breeding of silkworms in the future.

关键词： aquaporin Bombyx mori development mutant piggyBac silk gland

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