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Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive Detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification

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Engineering 2022年第12期19卷 62-70页

作者： Yuting Shang Shuzhen Cai Qinghua Ye Qingping Wu Yanna Shao Xiaoying Qu Xinran Xiang Baoqing Zhou Yu Ding Moutong Chen Liang Xue Honghui Zhu Jumei Zhang guangdong Provincial Key Laboratory of Microbial Safety and Health&State Key Laboratory of Applied Microbiology Southern China&institute of Microbiology Guangdong Academy of SciencesGuangzhou 510070China State Key Laboratory of Food Science and Technology&National Engineering research Center for Functional Foods&Synergetic Innovation Center of Food Safety&Joint International research Laboratory on Food Safety School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food *** this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)*** dot nanobeads(QBs)served as fluorescence reporters,providing good detection *** customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product *** was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)*** linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test *** specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and *** detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis.

关键词： Salmonella Typhimurium Quantum dot nanobeads Lateral flow immunoassay strip Loop-mediated isothermal amplification Strand displacement probe

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The pharmacological mechanism and molecular details of Platycodon grandiflorum in the treatment of novel coronavirus Pneumonia(COVID-19)

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Medical Data Mining 2023年第1期6卷 37-52页

作者： Hao-Wen Lin Qu Wang Lian-Xiang Luo The First Clinical College Guangdong Medical UniversityZhanjiangGuangdong 524023China Southern Marine Science and Engineering guangdong Laboratory(zhanjiang) ZhanjiangGuangdong 524023China The Marine Biomedical research institute Guangdong Medical UniversityZhanjiangGuangdong 524023China The Marine Biomedical research institute of guangdong zhanjiang ZhanjiangGuangdong 524023China

Background:Novel coronavirus pneumonia(COVID-19)has developed as a pandemic of global *** is an urgent need to develop effective and safe *** grandiflorum(PG),one of the most famous traditional Chinese herbs,may be *** this study,we explored the pharmacological mechanism of PG in the treatment of ***:The active compounds and potential targets were acquired from public *** protein-protein interaction,the Gene Ontology,and the Kyoto Encyclopedia of Genes and Genomes were determined through bioinformatics *** docking and molecular dynamics were performed to further verify the ***:A total of 38 bioactive ingredients and 276 gene targets of PG were *** were 78 intersected targets of PG and *** network analysis revealed that luteolin,Platycogenic acid A,Platycogenic acid C,Polygalacic acid,and acacetin may be candidate *** AKT1,VEGFA,TP53,MAPK3,TNF,IL6,CASP3,EGFR,STAT3,and CCND1 were the important potential drug *** Ontology terms are involved in biological processes,which are mainly concentrated in inorganic substances and apoptosis,*** Kyoto Encyclopedia of Genes and Genomes pathway was involved in several aspects,such as Virus infection and immune regulation-related *** docking results showed that compounds of PG are closely bound to related *** dynamics further found that Robin,Flavplatycoside,and dimethyl 3-O-β-D-glucopyranosylplatycogenate A can maintain good stability and flexibility in the composite ***:PG has multicomponent,multitarget,and multichannel characteristics,which can provide an important theoretical basis to treat patients with COVID-19.

关键词： Platycodon grandiflorum COVID-19 network pharmacology molecule docking molecular dynamics

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Development Strategy of Sugarcane Industry in guangdong Province

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Asian Agricultural research 2012年第10期4卷 17-22,27页

作者： CHEN Yue-gui WU Jian-tao YANG Jun-xian LI Qi-wei XIE Jing PAN Fang-yin WU Wen-long LIU Fu-ye DENG Hai-hua QI Yong-wen Guangzhou Sugarcane Industry research institute/guangdong Key Laboratory of Sugarcane Improvement & Biorefinery

guangdong Province is one of the main producing areas of sugarcane in China, and one of China's three regions with dominant advantage in sugarcane, having good basis and conditions for developing sugarcane industry. In this research, using the SWOT-AHP method, we set 20 assessment indicators (such as the regions with dominant advantage in sugarcane, huge demand for domestic sugar, increased production costs and backward system of sugarcane), to analyze the development strategy of sugarcane industry in guangdong Province, from strengths, weaknesses, opportunities and challenges of sugarcane industrial development in guangdong Province. The results show that in order to promote the development of sugarcane industry in guangdong Province, it is necessary to adopt the SO development strategy (relying on its own strengths and using favorable external environment), to achieve the rapid development.

关键词： guangdong Province Sugarcane industry Development

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The influence of turbulent mixing on the subsurface chlorophyll maximum layer in the northern South China Sea

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Journal of Oceanology and Limnology 2021年第6期39卷 2167-2180页

作者： Chenjing SHANG Changrong LIANG Guiying CHEN Yongli GAO Shenzhen Key Laboratory of Marine Bioresources and Eco-environmental Science College of Life Science and OceanographyShenzhen UniversityShenzhen 518060China State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering guangdong Laboratory(Guangzhou) Guangzhou 511458China equipment Public Service Center South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China

We present observations from deployments of turbulent microstructure instrument and CTD package in the northern South China Sea from April to May *** them we determined the turbulent mixing(dissipation rateεand diapycnal diffusivityκ),nutrients(phosphate,nitrate,and nitrite),nutrient fluxes,and chlorophyll a in two transects(A and B).Transect A was located in the region where turbulent mixing in the upper 100 m was weak(κ~10-6-10-4 m^(2)/s).Transect B was located in the region where the turbulent mixing in the upper 100 m was strong(κ~10-5-10-3 m^(2)/s)due to the influence of internal waves originating from the Luzon Strait and water intrusion from the Western *** both transects,there was a thin subsurface chlorophyll maximum layer(SCML)(>0.25 mg/m^(3))nested in the upper 100 *** observations indicate that the effects of turbulent mixing on the distributions of nutrients and chlorophyll a were different in the two *** the transect A with weak turbulent mixing,nutrient fluxes induced by turbulent mixing transported nutrients to the SCML but not to the upper *** were sufficient to support a local SCML phytoplankton population and the SCML remained *** the transect B with strong turbulent mixing,nutrient fluxes induced by turbulent mixing transported nutrients not only to the SCML but also to the upper water,which scatters the nutrients in the water column and diffuses the SCML.

关键词： turbulent mixing diapycnal diffusivity nutrients nutrient flux chlorophyll a

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MCTSR:A super-resolution method for defects in gas-insulated switchgear X-ray images based on multi-scale contextual transformers

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High Voltage 2023年第3期8卷 504-513页

作者： Guote Liu Jinhui Zhou Linqiang Xu Licheng Li Bing Luo Sijun Chen School of Mechatronic Engineering and Automation Foshan UniversityFoshanChina School of Electric Power Engineering South China University of TechnologyGuangzhouChina research institute of China Southern Power Grid Co. Ltd.GuangzhouChina guangdong Shuangdian Technology Co. Ltd.DongguanChina

As the core equipment of transmission and distribution hubs,the operational status of gas-insulated switchgear(GIS)is closely linked to the safety of the power ***,X-ray digital imaging technology has been extensively used in GIS equipment fault ***,the X-ray image of GIS is blurred,which is not conducive to the detection of tiny ***,a super-resolution method for GIS X-ray images based on multi-scale context transformers is proposed in this study,namely ***,a second-order image degradation model is introduced to generate GIS X-ray lowresolution images that more closely resemble the real ***,a contextual transformer gate module is constructed to improve attention to tiny defects in GIS X-ray ***,a U-Net discriminator network based on multi-scale contextual transformers is intended to enrich the information of the generated ***,the proposed discriminator is combined with the existing generator to compose a superresolution method applicable to GIS X-ray *** experimental results demonstrate that the method outperforms other methods in peak signal-to-noise ratio and structural similarity on the constructed GIS X-ray image *** addition,the output image of the proposed method facilitates the subsequent defect detection.

关键词： image resolution contextual

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High sensitivity conductivity-temperature-depth sensing based on an optical microfiber coupler combined fiber loop

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Chinese Optics Letters 2020年第1期18卷 34-39页

作者： Liang Ca Yang Yu Min Xiao Junbo Yang Xueliang Zhang Zhou Meng College of Meteorology and Oceanology National University of Defense TechnologyChangsha 410073China Unit 45 No.91388 Troops of PLAZhanjiang 524022China College of Liberal Arts and Sciences National University of Defense TechnologyChangsha 410073China Hunan Aerospace institute of Electromechanical Devices and special Material Changsha 410073China

In order to meet the practical needs of all-fiber conductivity-temperature-depth sensors with high sensitivity,compact structure,and easy packaging,this Letter uses a microfiber coupler combined with fiber loop(MCFL)reflective photonic device to conduct salinity,temperature,and deep sensing *** MCFLs’dynamic range and resolution of salinity,temperature,and depth can meet the requirements of actual marine environment *** structure opens up a new design idea for the practical research of microfiber coupler-based marine environmental parameter sensors.

关键词： microfiber coupler(MC) fiber loop salinity temperature and depth sensing cross sensitivity conductivity-temperature-depth measurement system

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Neuroprotectants attenuate hypobaric hypoxia-induced brain injuries in cynomolgus monkeys

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Zoological research 2020年第1期41卷 3-19页

作者： Pei Zhang Jie-Si Chen Qi-Ye Li Long-Xiang Sheng Yi-Xing Gao Bing-Zheng Lu Wen-Bo Zhu Xiao-Yu Zhan Yuan Li Zhi-Bing Yuan Gang Xu Bi-Tao Qiu Min Yan Chun-Xue Guo You-Qiong Wang Yi-Jun Huang Jing-Xia Zhang Fu-Yu Liu Zhong-Wei Tang Sui-Zhen Lin David NCooper Huan-Ming Yang Jian Wang Yu-Qi Gao Wei Yin Guo-Jie Zhang Guang-Mei Yan State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China BGI-Shenzhen ShenzhenGuangdong 518083China Section for Ecology and Evolution Department of BiologyUniversity of CopenhagenCopenhagen DK-2100Denmark Department of Pharmacology Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdong 510080China institute of Medicine and Hygienic equipment for High Altitude Region College of High Altitude Military MedicineThird Military Medical UniversityChongqing 400038China Key Laboratory of High Altitude Medicine of People's Liberation Army Chongqing 400038China Key Laboratory of High Altitude Environmental Medicine Third Military Medical UniversityMinistry of EducationChongqing 400038China School of Pharmaceutical Sciences Sun Yat-Sen UniversityGuangzhouGuangdong 510006China Guangzhou Cellprotek Pharmaceutical Co.Ltd. GuangzhouGuangdong 510663China institute of Medical Genetics School of MedicineCardiff UniversityCardiff CF144XNUK James D.Watson institute of Genome Sciences HangzhouZhejiang 310058China Department of Biochemistry Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhouGuangdong 510080China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China China National Genebank BGI-ShenzhenShenzhenGuangdong 518120China

Hypobaric hypoxia (HH) exposure can cause serious brain injury as well as life-threatening cerebral edema in severe cases. Previous studies on the mechanisms of HH-induced brain injury have been conducted primarily using non-primate animal models that are genetically distant to humans, thus hindering the development of disease treatment. Here, we report that cynomolgus monkeys (Macaca fascicularis) exposed to acute HH developed human-like HH syndrome involving severe brain injury and abnormal behavior. Transcriptome profiling of white blood cells and brain tissue from monkeys exposed to increasing altitude revealed the central role of the HIF-1 and other novel signaling pathways, such as the vitamin D receptor (VDR) signaling pathway, in co-regulating HH-induced inflammation processes. We also observed profound transcriptomic alterations in brains after exposure to acute HH, including the activation of angiogenesis and impairment of aerobic respiration and protein folding processes, which likely underlie the pathological effects of HH-induced brain injury. Administration of progesterone (PROG) and steroid neuroprotectant 5α-androst-3β,5,6β-triol (TRIOL) significantly attenuated brain injuries and rescued the transcriptomic changes induced by acute HH. Functional investigation of the affected genes suggested that these two neuroprotectants protect the brain by targeting different pathways, with PROG enhancing erythropoiesis and TRIOL suppressing glutamate-induced excitotoxicity. Thus, this study advances our understanding of the pathology induced by acute HH and provides potential compounds for the development of neuroprotectant drugs for therapeutic treatment.

关键词： Acute hypobaric hypoxia Cynomolgus monkeys Brain injury Neuroprotectant Gene regulatory networks

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OmicsSuite: a customized and pipelined suite for analysis and visualization of multi-omics big data

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Horticulture research 2023年第11期10卷 13-26页

作者： Ben-Ben Miao Wei Dong Yi-Xin Gu Zhao-Fang Han Xuan Luo Cai-Huan Ke Wei-Wei You State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen 361102FujianChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-Sen UniversityGuangzhou 510055GuangdongChina College of Fisheries Guangdong Ocean UniversityZhanjiang 524088GuangdongChina Fujian institute for Sustainable Oceans Xiamen UniversityXiamen 361102FujianChina

With the advancements in high-throughput sequencing technologies such as Illumina,PacBio,and 10X Genomics platforms,and gas/liquid chromatography-mass spectrometry,large volumes of biological data in multiple formats can now be obtained through multi-omics *** is constantly evolving and seeking breakthroughs to solve multi-omics problems;however,it is challenging for most experimental biologists to analyse data using command-line interfaces,coding,and *** on experience with multi-omics,we have developed OmicsSuite,a desktop suite that comprehensively integrates statistics and multi-omics analysis and *** suite has 175 sub-applications in 12 categories,including Sequence,Statistics,Algorithm,Genomics,Transcriptomics,Enrichment,Proteomics,Metabolomics,Clinical,Microorganism,Single Cell,and Table *** created the user interface with Sequence View,Table View,and intelligent components based on JavaFX and the popular Shiny *** multi-omics analysis functions were developed based on BioJava and 300+packages provided by the R CRAN and Bioconductor communities,and it encompasses over 3000 adjustable parameter *** can directly read multi-omics raw data in FastA,FastQ,Mutation Annotation Format,mzML,Matrix,and HDF5 formats,and the programs emphasize data transfer directions and pipeline analysis *** can produce pre-publication images and tables,allowing users to focus on biological *** offersmulti-omics step-by-step workflows that can be easily applied to horticultural plant breeding and molecular mechanism studies in *** enables researchers to freely explore the molecular information contained in multi-omics big data(Source:https://***/OmicsSuite/,Website:https://***,v1.3.9).

关键词： breakthrough tables seeking

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The impact of ocean data assimilation on seasonal predictions based on the National Climate Center climate system model

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Acta Oceanologica Sinica 2021年第5期40卷 58-70页

作者： Wei Zhou Jinghui Li Fanghua Xu Yeqiang Shu Yang Feng equipment Public Service Center South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern Marine Science and Engineering guangdong Laboratory(Guangzhou) Guangzhou 511458China Ministry of Education Key Laboratory for Earth System Modeling Department of Earth System ScienceTsinghua UniversityBeijing 100084China State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China

An ensemble optimal interpolation(EnOI)data assimilation method is applied in the BCCCSM1.1 to investigate the impact of ocean data assimilations on seasonal forecasts in an idealized twin experiment *** of sea surface temperature(SST),sea surface height(SSH),sea surface salinity(SSS),temperature and salinity(T/S)profiles were first generated in a free model ***,a series of sensitivity tests initialized with predefined bias were conducted for a one-year period;this involved a free run(CTR)and seven assimilation *** tests allowed us to check the analysis field accuracy against the"truth".As expected,data assimilation improved all investigated quantities;the joint assimilation of all variables gave more improved results than assimilating them ***-year predictions initialized from the seven runs and CTR were then conducted and *** forecasts initialized from joint assimilation of surface data produced comparable SST root mean square errors to that from assimilation of T/S profiles,but the assimilation of T/S profiles is crucial to reduce subsurface *** ocean surface currents in the tropics were better predicted when initial conditions produced by assimilating T/S profiles,while surface data assimilation became more important at higher latitudes,particularly near the western boundary *** predictions of ocean heat content and mixed layer depth are significantly improved initialized from the joint assimilation of all the ***,a central Pacific El Ni?o was well predicted from the joint assimilation of surface data,indicating the importance of joint assimilation of SST,SSH,and SSS for ENSO predictions.

关键词： global ocean data assimilation EnOI twin experiments

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Volatile organic compounds in wintertime North China Plain:Insights from measurements of proton transfer reaction time-of-flight mass spectrometer(PTR-ToF-MS)

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Journal of Environmental Sciences 2022年第4期34卷 98-114页

作者： Xianjun He Bin Yuan Caihong Wu Sihang Wang Chaomin Wang Yibo Huangfu Jipeng Qi Nan Ma Wanyun Xu Ming Wang Wentai Chen Hang Su Yafang Cheng Min Shao institute for Environmental and Climate research Jinan UniversityGuangzhou 511443China guangdong-Hongkong-Macao Joint Laboratory of Collaborative Innovation for Environmental Quality Guangzhou 511443China State Key Laboratory of Severe Weather&Key Laboratory for Atmospheric Chemistry of China Meteorology Administration Chinese Academy of Meteorological SciencesBeijing 100081China Collaborative Innovation Center of Atmospheric Environment and equipment Technology Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution ControlSchool of Environmental Science and EngineeringNanjing University of Information Science&TechnologyNanjing 210044China Nanjing Intelligent Environmental Science and Technology Co. Ltd.Nanjing 211800China Max Planck institute for Chemistry Mainz 55128Germany

The characteristics of wintertime volatile organic compounds(VOCs)in the North China Plain(NCP)region are complicated and remain *** measurements were conducted by a proton transfer reaction time-of-flight mass spectrometer(PTR-ToF-MS)at a rural site in the NCP from November to December *** ions measured by PTRToF-MS were quantified and the overall VOC compositions were investigated by combining the measurements of PTR-ToF-MS and gas chromatography-mass spectrometer/flame ionization detector(GC-MS/FID).The measurement showed that although atmospheric VOCs concentrations are often dominated by primary emissions,the secondary formation of oxygenated VOCs(OVOCs)is non-negligible in the wintertime,i.e.,OVOCs accounts for 42%±7%in the total VOCs(151.3±75.6 ppbV).We demonstrated that PTR-MS measurements for isoprene are substantially overestimated due to the interferences of *** chemical changes of organic carbon in a pollution accumulation period were investigated,which suggests an essential role of fragmentation reactions for large,chemically reduced compounds during the heavy-polluted stage in wintertime *** changes of emission ratios of VOCs between winter 2011 and winter 2018 in the NCP support the positive effect of“coal to gas”strategies in curbing air *** high abundances of some key species(*** aromatics)indicate the strong emissions of coal combustion in wintertime of *** ratio of naphthalene to C8 aromatics was proposed as a potential indicator of the influence of coal combustion on VOCs.

关键词： North China Plain Volatile organic compound Wintertime PTR-ToF-MS

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