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Effects of ultrasound co.bined technology on quality and volatile co.pound properties of chili sauce

food Bioscience 2023年第3期53卷 2462-2472页

作者： Tiantian Tang Min Zhang Chung Lim Law Yaping Liu State Key Laboratory of food Science and Resources Jiangnan University214122WuxiJiangsuChina Jiangsu Province International Joint Laboratory on Fresh food Smart Processing and Quality Monitoring Jiangnan University214122WuxiJiangsuChina China General Chamber of co.merce Key Laboratory on Fresh food Processing&Preservation Jiangnan University214122WuxiJiangsuChina Department of Chemical and Environmental Engineering Malaysia CampusUniversity of NottinghamSemenyih43500SelangorMalaysia guangdong galore food co. Ltd.528447ZhongshanGuangdongChina

In this study,the effect and mechanism of seven kinds of sterilization treatments,including ultrasound(US),pasteurization(PT),high temperature short time(HTST),hot air(HA),microwave(MW),ultraviolet(UV)and steam sterilization,on ***,*** and *** were *** was found that PT,HTST,MW,and steam sterilization gave ideal bactericidal effect and were subsequently,co.bined with ultrasound treatment and applied to chili *** results showed that ultrasound co.bined with microwave(US+MW)treatment was effective in preserving the maximum nutritional value and flavor of chili sauce while ensuring its *** total number of co.onies and co.iforms were not detected after US+MW ***,the co.tents of total polyphenol,capsaicin and 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical scavenging ability in the US+MW group were 11.10%,12.78%and 17.00%higher than those of traditional autoclave *** addition,polyphenol oxidase(PPO)activity was 2.04 times higher in the US+MW group co.pared to the autoclave sterilization *** analysis showed that total polyphenol and capsaicin were positively co.related with 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid(ABTS)(co.relation co.fficients 0.90 and 0.76,respectively)and DPPH free radical scavenging ability(co.relation co.fficients 0.88 and 0.72,respec-tively).The results obtained from GC-MS showed that US+MW treatment co.ld reduce the loss of volatile co.pounds and retained the original flavor of chili *** co.tent of diallyl disulfide was 1.8 times that of autoclave *** study provides a new theoretical reference for sterilization and flavor preservation of chili sauce.

关键词： Chili sauce Ultrasound Microwave Sterilization GC-MS Volatile co.pound

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γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation

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food Science and Human Wellness 2024年第5期13卷 3043-3053页

作者： Yao Wang Dongwei Wang Kai Wang Min Zhao Cizhou Li Yu Wang Xuwei Liu Lei Zhao Zhuoyan Hu guangdong Provincial Key Laboratory of food Quality and Safety College of Food ScienceSouth China Agricultural UniversityGuangzhou 510642China Guangzhou Wanglaoji Lychee Industry Development co. Ltd.Guangzhou 510623China

Previous research reported litchi thaumatin-like protein(LcTLP)co.ld lead to inflammation,which is a factor causing the adverse reactions after excessive intake of *** a main amino acid in litchi pulp,γ-aminobutyric acid(GABA)was found with anti-inflammatory ***,this study aimed to investigate the effects of GABA on LcTLP-induced inflammation through RAW264.7 macrophages and C57BL mice *** vitro study showed GABA co.ld effectively regulate the level of inflammatory cytokines(interleukin(IL)-1β,IL-6,IL-10,and prostaglandin E2)and Ca2+in cells,and inhibit the phosphorylation of p65,IκB,p38,c-Jun N-terminal kinase(JNK)and extracellular signal-regulated kinase(ERK).These results indicate GABA alleviated inflammation through nuclear factor-κB and mitogen-activated protein kinase pathway signaling *** vivo experiment was performed to verify the anti-inflammatory effect of GABA,and the results demonstrated that GABA reduced the inflammation and oxidative stress in the liver of LcTLP-treated mice,as it down-regulated the pro-inflammatory cytokines,malondialdehyde,aspartate transferase,and alanine *** relative expression of phosphorylated p38,JNK and ERK in mice liver with GABA treatment were reduced to 65%,39%and 80%of the co.trol group,***,GABA treatment enriched probiotic bacteria and decreased pathogenic bacteria in mice gut,which reveals GABA co.ld effectively reduce the translocation of gut microbiota.

关键词： Anti-inflammation Gut microbiota Litchi thaumatin-like protein Mitogen-activated protein kinase pathway γ-Aminobutyric acid

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A red pomegranate fruit extract-based formula ameliorates anxiety/depression-like behaviors via enhancing serotonin (5-HT) synthesis in C57BL/6 male mice

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food Science and Human Wellness 2021年第3期10卷 289-296页

作者： Xiping Zhu Dongxiao Sun-Waterhouse Chun Cui co.lege of food Science and Engineering South China University of TechnologyGuangzhou 510640GuangdongChina co.lege of Biological and food Engineering Anhui Polytechnic UniversityWuhu 241000AnhuiChina guangdong Weiwei Biotechnology co. LTD.Guangzhou 510640China

This study investigated the anti-anxiety/anti-depression potential of a formula co.taining red pomegranate fruit extract(RPFE;40%,m/m),Lactobacillus rhamnosus(JB-1)(34%,m/m),magnesium gluco.ate(25%,m/m)and vanillin(1%,m/m).The RPFE formula(dose:2.0,1.5 or 1.0 mg/g·day)reversed behavioral dysfunctions and body weight gain induced by chronic restraint stress co.bined with co.tico.terone injection in C57BL/6 male *** RPFE formula exhibited the abilities to normalize the levels of serum infl ammatory cytokines(NF-κB,TNF-α,IL-6,IL-1βand IFN-γ)and malondialdehyde(MDA),and activities of superoxide dismutase(SOD),catalase(CAT)and nitric oxide synthase(NOS),as well as relieve the injury of hippocampal *** serotonin(5-HT)levels in hippocampus were increasingly enhanced,which might be mediated by reducing the activity of indoleamine-2,3-dioxygenase(IDO)and increasing the activity of tryptophan hydroxylase(TPH).Thus,the neuroprotective and ameliorating effects on anxiety/depression-like behaviors resulting from the RPFE formula ingestion were possibly related to serotonergic activation,which might be mediated via anti-infl ammatory and anti-oxidant actions.

关键词： Behavioral tests Red pomegranate fruit extract 5-HT Anti-infl ammatory Anxiety/depression

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The neuroprotective effect of walnut-derived peptides against glutamate-induced damage in PC12 cells: mechanism and bioavailability

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food Science and Human Wellness 2022年第4期11卷 933-942页

作者： Shuguang Wang Lin Zheng Tiantian Zhao Qi Zhang Guowan Su Mouming Zhao School of food Science and Engineering South China University of TechnologyGuangzhou 510640China guangdong food Green Processing and Nutrition Regulation Technologies Research Center Guangzhou 510650China guangdong Huapeptides Biotechnology co. Ltd.Zhaoqing 526000China

In our previous study, defatted walnut meal hydrolysate(DWMH) co.ld attenuate D-galactose-induced acute memory deficits in vivo, and six potent active peptides including WSREEQ, WSREEQE, WSREEQEREE, ADIYTE, ADIYTEEAG and ADIYTEEAGR were identified. The aim of this study was to investigate the possible mechanism underlying their neuroprotective effects on glutamate-induced apoptosis in PC12 cells and their digestive stability. Results showed that all these peptides co.ld attenuate the reduction of cell viability caused by glutamate in PC12 cells, especially WSREEQEREE and ADIYTEEAGR. The addition of Arg residue in WSREEQEREE and ADIYTEEAGR might be the potential reason for their stronger protective effects. Additionally, these two peptides possibly protected PC12 cells against glutamate-induced apoptosis via activating intracellular antioxidant defence(superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px)) through Kelch-like ECH-associated protein 1(Keap1) inhibition, inhibiting ROS production, Ca;influx and mitochondrial membrane potential(MMP) co.lapse as well as regulating the expression of apoptosis-related proteins(Bax and Bcl-2). This might be due to the presence of Trp, Tyr and Arg in these two peptides. However, encapsulation of WSREEQEREE and ADIYTEEAGR should be co.sidered based on their digestive sensibility during in vitro gastrointestinal digestion.

关键词： Neuroprotective effects Walnut peptides PC12 cells Oxidative injury Digestive stability

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Effect of co.bined zinc oxide nanoparticles using citric acid and microwave heating on the quality of animal fat

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food Bioscience 2023年第3期53卷 1565-1575页

作者： Lan Zhang Min Zhang Yaping Liu Zhongxiang Fang State Key Laboratory of food Science and Technology School of Food Science and TechnologyJiangnan University214122WuxiJiangsuChina Jiangsu Province International Joint Laboratory on Fresh food Smart Processing and Quality Monitoring Jiangnan University214122WuxiJiangsuChina China General Chamber of co.merce Key Laboratory on Fresh food Processing&Preservation Jiangnan University214122WuxiJiangsuChina guangdong galore food co Ltd528447ZhongshanGuangdongChina School of Agriculture and food The University of MelbourneParkvilleVictoria3010Australia

The processing method of animal fat as a non-negligible food resource has attracted *** nanoparticles(ZnONPs)are a widely studied inorganic antimicrobial *** this paper,citric acid modified ZnO nanoparticles(CA-ZnO)were prepared using citric acid for surface modification of ZnO *** surface modification reduced the agglomeration of ZnONPs in the water,and the particle size of CA-ZnO particles was reduced by about 4 times co.pared to the ZnONPs after dispersion in the ***-ZnO also showed a good inhibitory effect against Bacillus cereus,Escherichia co.i,and Staphyloco.cus aureus at the co.centration of 0.05 g/*** heating co.bined with CA-ZnO(MCCZ)had a good bactericidal effect and disrupted the integrity of bacterial cell membrane leading to leakage of cell materials and altering the seco.dary structure of bacterial proteins such as the reduction ofα-*** this method for sterilization of animal fats(a mix of same amount of chicken oil and lard),the sterilization rate of animal fats reached 99.97%after 90 s of MCCZ *** MCCZ resulted in less flavor change and quality deterioration co.pared to traditional high temperature ***,MCCZ co.ld be used as a new method to preserve the product quality of mixed chicken oil and lard.

关键词： Citric acid ZnO nanoparticles Antibacterial activity Animal fat Lipid oxidation

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Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: a co.parison study

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food Science and Human Wellness 2022年第4期11卷 1028-1035页

作者： Junjie Lin Huan Xiang Dongxiao Sun-Waterhouse Chun Cui Wei Wang co.lege of food Science and Technology South China University of TechnologyGuangzhou 510640China guangdong Wei-Wei Biotechnology co. LtdGuangzhou 510640China

Seabuckthorn seed meal(SSM) is a waste of oil extraction industry that rich in protein. In order to seek suitable protein extraction method, three different deep eutectic solvents(DESs)(including choline chlorideglycerol, choline chloride-oxalic acid and choline chloride-urea) were developed for extracting protein from SSM and co.pared with alkaline. Result indicated that alkaline co.ld effectively extract 56.9% protein from SSM and its protein co.tent was 73.1%, higher than DES at 31.0%-41.4% and 64.3%-67.5%, respectively. However, co.pared to alkali, DES led to a product with less β-sheet, more β-turn, more essential amino acids, higher total amino acid co.tent, especially choline chloride-urea which extracted protein showing an integrated and similar protein weight distribution co.pared to SSM. Also, this protein extracted chloride-urea showed a highest digestibility in vitro(by pepsin)(54.2%). These results indicated that choline chloride-urea extraction is better than alkaline extraction for SSM.

关键词： Seabuckthorn seed meal Protein extraction Deep eutectic solvent Alkaline extraction and acid precipitation Amino acids

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Antioxidant Activities of Crude Phlorotannins from Sargassum hemiphyllum

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2016年第3期36卷 449-455页

作者：赵智丽杨小青龚忠勤潘明珠韩雅莉刘义 Department of food and Biology Guangdong University of Technology People’s Hospital of Longhua District School of Pharmacy Guangdong Medical College Shenzhen Enhance Technology co. Ltd. Key Laboratory of Research & Development of Nature Drug Guangdong Medical College

Brown algae are well known as a source of biologically active co.pounds, especially those having antioxidant activities, such as phlorotannins. In this study we examined the antioxidant activities of crude phlorotannins extracts（CPEs） obtained from Sargassum hemiphyllum（SH） and fractionated acco.ding to the molecular weights. When CPEs were administrated at a dose of 30 mg/kg to Kunming mice pre-treated with carbon tetrachloride（CCl4）, the levels of oxidative stress indicators in the liver, kidney and brain were significantly reduced in vivo. All the co.ponents of various molecular weight fractions of CPEs exhibited greater scavenging capacities in clearing hydroxyl free radical and superoxide anion than the positive co.trols gallic acid, vitamin C and vitamin E. Particularly, the co.ponents greater than 30 k D obtained from ethyl acetate phase showed the highest antioxidant capacities. These results indicated that SH is a potential source for extracting phlorotannins, the algal antioxidant co.pounds.

关键词： Sargassum hemiphyllum phlorotannins antioxidant activities in vitro in vivo

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Hypoglycemic polysaccharides from Auricularia auricula and Auricularia polytricha inhibit oxidative stress,NF-κB signaling and proinflammatory cytokine production in streptozotocin-induced diabetic mice

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food Science and Human Wellness 2021年第1期10卷 87-93页

作者： Huan Xiang Dongxiao Sun-Waterhouse Chun Cui School of food Science and Engineering South China University of TechnologyGuangzhou510640GuangdongChina guangdong Weiwei Biotechnology co. LTDGuangzhou510640GuangdongChina School of Chemical Sciences The University of AucklandPrivate Bag 92019AucklandNew Zealand

Auricularia auricula(AA)and Auricularia polytricha(AP)are popular edible *** study successfully produced hypoglycemic polysaccharides from un-smashed or smashing and sieving(through a 10-mesh sieve)AA and AP(termed as AAP/AAP-10 and APP/APP-10)via scalable processes(water extraction,ethanolic precipitation and deproteinization).This is the first report to co.pare the effectiveness of AAP and APP in co.bating streptozotocin-induced oxidative stress and diabetes-related changes in mice(body weight,fasting blood gluco.e,serum insulin,proinflammatory mediator and cytokines,oxidative stress-related products,antioxidant enzymes).APP and AAP with different molecular weights and monosaccharide molar ratios co.ld be therapeutic options for diabetes with a low dose(100 mg/kg/day)likely working *** the same dose,APP generally performed more effective than AAP,and AAP-10/APP-10 seemed slightly more beneficial than AAP/*** mechanism underlying these antidiabetic functions might involve the NF-κB and associated signalling *** is cheaper than AA,thereby representing a favorable source of functional polysaccharides.

关键词： Polysaccharides Auricularia auricula Auricularia polytricha Hypoglycemic effect Oxidative stress

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Developmental changes in lipid and fatty acid metabolism and the inhibition by in ovo feeding oleic acid in Musco.y duck embryogenesis

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Animal Nutrition 2023年第1期12卷 321-333页

作者： Xiufen Zhang Qilin Wu Wenxuan Zheng Chuang Liu Liang Huang Xin Zuo Wenquan Xiao Xiaofeng Han Hui Ye Wence Wang Lin Yang Yongwen Zhu guangdong Provincial Key Laboratory of Animal Nutrition and Regulation College of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China Wen's food Group co. LtdYunfu 52740China

Hepatic lipid and fatty acid(FA)metabolism are critical for regulating energetic homeostasis during *** present,it remains unclear how an exogenous FA intervention affects embryonic development in an avian embryo *** Exp.1,30 fertilized eggs were sampled on embryonic days(E)16,19,22,25,28,31 and the day of hatch(DOH)to determine the critical period of lipid *** Exp.2,a total of 120 fertilized eggs were divided into two groups(60 eggs/group)for in ovo feeding(IOF)procedures on *** were injected into the yolk sac with PBS as the co.trol group and with oleic acid(OA)as the IOF-OA treatment *** were co.lected on E28 and *** Exp.1,hepatic triacylglycerol(TG)and cholesterol(CHO)co.tents increased while serum TG co.tent decreased from E16 to DOH(Pco.pared with the co.trol embryos,IOF-OA embryos had an increased yolk sac TG co.tent on E28 and E31,and a decreased serum TG and CHO co.tent on E28(Pco.trol embryos(Pco.trol embryos(P0.05).Maximal metabolic changes in lipid and FA metabolism occurred on E22-E28 in Musco.y duck embryogenesis,along with the altered target gene and protein expression related to lipogenesis and ***-OA intervention on E25 co.ld inhibit the target gene expression related to FA uptake,synthesis,and oxidation,which may influence the normal FA metabolism on E28 during embryogenesis.

关键词： Embryo Lipid Triacylglycerol Cholesterol Fatty acid Oleic acid

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Changes of visceral properties and digestive enzymes in the herbivorous marine teleost Siganus canaliculatus fed on different diets

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Acta Oceanologica Sinica 2018年第2期37卷 85-93页

作者： XIE Dizhi XU Shude WU Qingyang CHEN Fang WANG Shuqi YOU Cuihong LI Yuanyou co.lege of Marine Sciences South China Agricultural University guangdong Provincial Key Laboratory of Marine Biotechnology Shantou University guangdong VTR Bio-tech co. Ltd

The rabbitfish Siganus canaliculatus is one of the few cultured herbivorous marine teleosts. To better understand the digestive physiology of this fish and provide data for designing formulated feed using macroalgae as an ingredient, the changes of visceral properties and digestive enzyme activities were investigated after the juveniles were fed on different types of food including raw fish（RF）, formulated diet（FD） or macroalgae Enteromorpha prolifra（EP） and Gracilaria lemaneiformis（GL） for eight weeks. The results showed that the hepatosomatic and viscerosomatic indices in the RF and FD groups, as well as the relative intestine length（RIL） in the EP and GL groups, were significantly higher than those in other groups. Additionally, differences in the histological structure of the liver and anterior intestine were also observed among different dietary groups. The hepatic nuclei were displaced to the periphery by lipid inclusions in fish fed RF. The highest levels of muco.al folds were found in the anterior intestines of fish fed macroalgae. Digestive enzyme activity profiles showed obvious fluctuations in the first three weeks, and then leveled off in the following weeks. The levels of protease, lipase and α-amylase in the alimentary tract showed changes related to the levels of dietary protein, lipid and carbohydrate, *** macroalgae significantly inhibited the activity of protease in the stomach, it increased RIL and the number of muco.al folds in the anterior intestine so as to co.pensate for the influences on protease activities in the stomach. This study suggests that the digestive tract of rabbitfish can well adapt to different diets, and needs about three weeks to physiologically acclimatize to the nutritional status, thus implying that rabbitfish are somewhat omnivorous.

关键词： digestive enzymes macroalgae Siganus canaliculatus visceral property

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