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cysA,cysP,and rpoS mutations increase the power density in *** microbial fuel cells:Performing enhancement based on metabolic flux analysis

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Advances in Bioscience and Biotechnology 2013年第1期4卷 103-111页

作者： Juan Diego Mejia Cindy Stephany Rojas Laura Avellaneda Franco David Alejandro Urbina Gomez Beatriz Helena Correa Arias Nubia Milena Velasco Rodriguez Maria Teresa Cortes Montanez Martha Josefina Vives-Florez Andres Fernando Gonzalez Barrios grupo de Diseno de Productos y Procesos(GDPP) Departamento de Ingenieria QuimicaUniversidad de los AndesBogotaColombia grupo de Investigacion en Produccion y Logistica(PYLO) Departamento de Ingenieria IndustrialUniversidad de los AndesBogotaColombia Laboratorio de Electroquimica y Materiales Polimericos Departamento de QuimicaUniversidad de los AndesBogotaColombia Centro de Investigaciones Microbiologicas(CIMIC) Departamento de Ciencias BiologicasUniversidad de los AndesBogotaColombia

This study presents the simulation of a MFC with Pseudomonas aeruginosa based on a metabolic flux analysis (MFA) which arises as a linear programming model that served as input for the fuel cell model. The linear model was implemented in Xpress MP? and the coupling model in Comsol Multiphysics?. The in silico model predicted maximum potentials of 0.135 V which were consistent with those obtained in the experimental cell. Afterwards, an optimization platform based on multiobjective optimization approach was implemented aimed to determine genes that increase the average cell power. cysA, cysP and rpoS mutants which were predicted to increase the power of the cell, were experimentally tested with an air cathode fuel cell finding an augment up to 35-fold in the average power density for the rpoS mutant. Power densities were obtained through experimentation in the range of [0.13 - 5] mW/m2. Electron shuttle rise was qualitative corroborated trough cyclic voltammetry tests, which allowed to visualize the augment of the peaks for rpoS mutation.

关键词： Component Formatting Style Styling Insert

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Infrared fluorescence imaging of infarcted hearts with A@S nanodots

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Nano Research 2019年第4期12卷 749-757页

作者： Dirk H. Ortgies Angel Luis Garcia-Villalon Miriam Granado Sara Amor Emma Martin Rodriguez Harrisson DA Santos Jingke Yao Jorge Rubio-Retama Daniel Jaque Nanobiology Group Instituto Ramon y Cajal de Investigacion Sanitaria IRYCIS Ctra. Colmenar km. 9.100 Madrid 28034 Spain Fluorescence Imaging Group Departamento de Fisica de Materiales - Facultad de Ciencias Universidad Autonoma de Madrid C/ Francisco Tomas y Valiente 7 Madrid 28049 Spain Fluorescence Imaging Group Departamento de Fisiologia - Facultad de Medicina Avda. Arzobispo Morcillo 2 Universidad Autonoma de Madrid Madrid 28029 Spain Fluorescence Imaging Group Departamento de Fisica Aplicada - Facultad de Ciencias Universidad Autonoma de Madrid C/ Francisco Tomas y Valiente 7 Madrid 28049 Spain grupo de Nano-Fotonica e Imagens Instituto de Fisica Universidade Federal de Alagoas Maceio-AL 57072-900 Brazil departamento de quimica Fisica en Ciencias Farmaceuticas Facultad de Farmacia Plaza de Ramon y Cajal s/n Universidad Complutense de Madrid Madrid 28040 Spain

Ag2S nano dots have already bee n dem onstrated as promisi ng n ear-i nfrared (NIR-II, 1.0-1.45 pm) emitting nan oprobes with low toxicity, high penetration and high resolution for in vivo imaging of, for example, tumors and vasculature. In this work, we have systematically investigated the pote ntial applicati on of function alized Ag2S nano dots for accurate imaging of damaged myocardium tissues after a myocardial in farction in duced by either partial or global ischemia. Ag2S nano dots surface-function alized with the an giote nsin II peptide (ATI I) have show n over 10-fold enhanced binding efficiency to damaged tissues than non-specifically (PEG) functionalized Ag2S nanodots due to their interaction with the upregulated angiotensin II receptor type I (AT1R). It is demonstrated how the NIR-II images generated by ATII-functionalized Ag2S nanodots contain valuable information about the location and extension of damaged tissue in the myocardium allowing for a proper identification of the occluded artery as well as an in direct evaluati on of the damage level. The pote ntial applicati on of Ag2S nano dots in the n ear future for in vivo imaging of myocardial infarction was also corroborated by performing proof of concept whole body imaging experiments.

关键词： infrared imaging myocardial infarct Ag2S nanodots biological windows Langendorff heart

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Probing behavior of aposymbiotic green peach aphid persicae） on susceptible Solanum tuberosum and resistant Solanum stoloniferum plants

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Insect Science 2018年第1期25卷 127-136页

作者： Cristina Renata Machado-Assefh Adriana Elisabet Alvarez Catedra de quimica Biologica Facultad de Ciencias Naturales Universidad Nacional de Salta Avda Bolivia 5150 A4408FVY Salta Argentina Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) CCT Salta Avda Bolivia 5150 A4408FVY Salta Argentina

The green peach aphid, Myzuspersicae Sulzer （Hemiptera： Aphididae） is one of the potato important pests; it is the most efficient vector of potato viruses. Myzuspersicae harbors the endosymbiotic bacteria Buchnera aphidicola which supplements their diet. There is increasing evidence that B. aphidicola is involved in plant-aphid interactions and we previously demonstrated that B. aphidieola disruption （aposymbiosis） affected the probing behavior ofM. persicae on radish plants, delaying host plant acceptance. In this work, we evaluated the effect of aposymbiosis on the probing behavior of M. persicae on 2 Solanum species with different compatibility with M. persieae, Solanum tuberosum （susceptible） and Solanum stoloniferum （resistant） with the electrical penetration graph technique （EPG）. To disrupt B. aphidicola, rifampicin was administered to aphids through artificial diets. Aposymbiotic aphids, on both plant species, showed increased pathway activities, mechanical problems with the stylets, and delayed salivation in the phloem. The extended time in derailed stylet mechanics affected the occurrence of most other probing activities; it delayed the time to the first phloem phase and prevented ingestion from the phloem. The effect of aposymbiosis was more evident in the compatible interaction of M. persicae-S, tuberosum, than in the incompatible interaction with S. stoloniferum, which generated the M. persicae--S, tuberosum interaction to become incompatible. These results confirm that B. aphidicola is involved in the plant-aphid interaction in relation to plant acceptance, presumably through a role in stylets penetration in the plant.

关键词： aphid artificial diets Buchnera aphidicola EPG technique potato

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Apaf1 inhibition promotes cell recovery From apoptosis

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Protein & Cell 2015年第11期6卷 833-843页

作者： Anna Gortat Monica Sancho Laura Mondragon Angel Messeguer Enrique Perez-Paya Mar Orzaez Laboratory of Peptide and Protein Chemistry Centro de Investigaci6n Principe Felipe 46012 Valencia Spain department of Chemical and Biomolecular Nanotechnology Instituto Quimica Avanzada de CataluSa (CSIC) 08034 Barcelona Spain Instituto de Biomedicina de Valencia IBV-CSIC 46010 Valencia Spain

The protein apoptoUc protease activating factor I （Apafl） is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apafl, cells recover from doxorubicinor hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apafl not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.

关键词： Apaf1 Apaf1 inhibitors apoptosis,apoptosome autophagy cell recovery

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Filling the Gaps to Solve the Extensin Puzzle

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Molecular Plant 2018年第5期11卷 645-658页

作者： Eliana Marzol Cecilia Borassi Mauro Bringas Ana Sede Diana Rosa Rodriguez Garcia Luciana Capece Jose M. Estevez Fundacion Instituto Leloir and Instituto de Investigaciones Bioquimicas de Buenos Aires (IIBBA-CO.NICEr) Avenida Patricias Argentinas 435 Buenos AiresCP C1405BWE Argentina departamento de quimica Inorganica Analitica y Quimica Fisica Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires (INQUIMAE-CONICET)Buenos Aires CP C1428EGA Argentina Instituto de Investigaciones en Ingenierfa Genetica y Biologia Molecular Dr. Hector Torres (INGEBI-CONICET) Vuelta de Obligado 2490 Buenos Aires C1428ADNArgentina

Extensins （EXTs） are highly repetitive plant O-glycoproteins that require several post-translational modifi- cations （PTMs） to become functional in plant cell walls. First, they are hydroxylated on contiguous proline residues; then they are O-glycosylated on hydroxyproline and serine. After secretion into the apoplast, O-glycosylated EXTs form a tridimensional network organized by inter- and intra-Tyr linkages. Recent studies have made significant progress in the identification of the enzymatic machinery required to process EXTs, which includes prolyl 4-hydroxylases, glycosyltransferases, papain-type cysteine endopeptidases, and peroxidases. EXTs are abundant in plant tissues and are particularly important in rapidly expanding root hairs and pollen tubes, which grow in a polar manner. Small changes in EXT PTMs affect fastgrowing cells, although the molecular mechanisms underlying this regulation are unknown. In this review, we highlight recent advances in our understanding of EXT modifications throughout the secretory pathway, EXT assembly in cell walls, and possible sensing mechanisms involving the Catharanthus roseus cell surface sensor receptor-like kinases located at the interface between the apoplast and the cytoplasmic side of the plasma membrane.

关键词： Arabidopsis thaliana cysteine endopeptidases extensins glycosyltransferases peroxidases prolyl 4-hydroxylases

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Fast determination of Benzodiazepines in Human Urine via Liquid-Liquid Extraction with Low Temperature Partitioning and LC-HRMS

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American Journal of Analytical Chemistry 2012年第2期3卷 118-124页

作者： Elisangela Jaqueline Magalhaes Clesia Cristina Nascentes Rodinei Augusti Maria Eliana Lopes Ribeiro de Queiroz Julio Cesar Cardoso da Silva Robson Jose de Cassia Franco Afonso departamento de quimica Universidade Federal de Minas GeraisBelo HorizonteBrazil departamento de quimica Universidade Federal de VicosaVicosaBrazil departamento de quimica Universidade Federal de Ouro PretoOuro PretoBrazil

A simple and high-throughput method to simultaneously determine selected benzodiazepines (i.e., diazepam, lorazepam, clonazepam, and bromazepam) in urine was developed and validated. The entire methodology consisted of the application of an innovative extraction/cleanup procedure, namely liquid-liquid extraction with low-temperature partitioning (LLE-LTP), and analysis by liquid chromatography combined with high-resolution mass spectrometry (LC-HRMS). The LLE-LTP procedure was optimized via factorial design and by evaluating crucial variables, specifically the freezing mode (either slow or fast), the urine/acetonitrile volume ratio, and the sample ionic strength. The benzodiazepines were quantified using matrix-matched calibration curves where the following parameters were assessed by validation protocol: in general, linearity range of 17 - 200 μg?L–1 (r > 0.9957);limits of detection lower than 5 μg?L–1;relative standard deviations (RSD) lower than 12.5%;and accuracy ranging from 72.3 to 117%. To test this procedure’s performance, the method was applied to determine the content of diazepam in actual urine samples. The validation results obtained for the method demonstrated that the present methodology could be potentially applied in proficient laboratories as a routine approach for determining benzodiazepines compounds content in urine.

关键词： Benzodiazepines Urine LC-HRMS Extraction Procedure Low-Temperature Partitioning

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Cadmium sorption by sodium and thiourea-modified zeolite-rich tuffs

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Journal of Environmental Sciences 2017年第2期29卷 39-48页

作者： Perfecto Barragán P M.Guadalupe Macedo M Maria T.Olguín Instituto Tecnologico de Toluca Laboratorio de Investigacion en Ingenieria Ambiental.Av.Tecnologico S/NColonia Ex-Rancho La VirgenMetepecEstado de Meico CP.52140Mexico Instituto National de Investigaciones Nucleares Departamento de QuimicaA.P.18-1027Col.EseandonDelegacion Miguel Hidalgo C.P.11801Mexico

In the present investigation, two zeolite-rich tuffs from Guaymas and El Cajon（State of Sonora）, which were conditioned with a sodium chloride solution and subsequently modified with a thiourea solution, were chosen to evaluate the removal of Cd from aqueous systems. The zeolitic materials were characterized by scanning electron microscopy and X-ray diffraction. The surface area was also determined. The experiments were performed in a batch system, and the influences of p H, contact time between phases, and the concentration of Cd in the solution on the adsorption by sodium or thiourea-modified zeolite-rich tuff were investigated. It was found that the efficiency of Cd ion removal from aqueous solutions is influenced by the p H of the aqueous systems. The Cd adsorption kinetic data were well fitted to a pseudo-second-order model in all cases. The Langmuir and Langmuir–Freundlich isotherms adequately described the Cd sorption behavior by the zeolite from El Cajon pretreated with Na Cl and the zeolite from Guaymas modified with thiourea, respectively.

关键词： Cadmium Zeolite-rich-tuff Sorption Kinetics Isotherms

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Mobility of Arsenic and Heavy Metals in a Sandy-Loam Textured and Carbonated Soil

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Pedosphere 2009年第2期19卷 166-175页

作者： I. GARC íA M. DIEZ F. MART íN M. SIMóN C. DORRONSORO departamento Edafologia y quimica Agricola CITE IIB Carretera Sacramento s/n 04120 University of Almeria (Spain) departamento Edafologia y quimica Agricola Campus Fuentenueva s/n 18002 University of Granada (Spain)

The continued effect of the pyrite-tailing oxidation on the mobility of arsenic, lead, zinc, cadmium, and copper was studied in a carbonated soil under natural conditions, with the experimental plot preserved with a layer of tailing covering the soil during three years. The experimental area is located in Southern Spain and was affected by a pyrite-mine spill. The climate in the area is typically Mediterranean, which determines the rate of soil alteration and element mobility. The intense alteration processes that occurred in the soil during three years caused important changes in its morphology and a strong degradation of the main soil properties. In this period, lead concentrated in the first 5 mm of the soil, with concentrations higher than 1500 mg kg?1, mainly associated to the neoformation of plumbojarosite. Arsenic was partially leached from the first 5 mm and mainly concentrated between 5–10 mm in the soil, with maximum values of 1239 mg kg-1; the retention of arsenates was related to the neoformation of iron hydroxysulfates (jarosite, schwertmannite) and oxyhydroxides (goethite, ferrihydrite), both with a variable degree of crystallinity. The mobility of Zn, Cd, and Cu was highly affected by pH, producing a stronger leaching in depth; their retention was related to the forms of precipitated aluminium and, in the case of Cu, also to the neoformation of hydroxysulfate.

关键词： arsenic heavy metals oxidation pyrite-tailing soil

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MODIFIED BENZOXAZINOIDS BASED ON ALLELOCHEMICALS FROM GRAMINEAE AS POTENTIAL RICE PROTECTION TOOL AGAINST ECHINOCHLOA CRUS-GALLI.

MODIFIED BENZOXAZINOIDS BASED ON ALLELOCHEMICALS FROM GRAMIN...

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中国第三届植物化感作用学术研讨会;第八届全国杂草科学大会;联合国粮农组织——中国“水稻化感作用论坛”

作者： Francisco A.MACIAS Jose M.G.MOLINILLO Rosa M.VARELA Nuria CHINCHILLA David MARIN Elena ARROYO grupo de Alelopatia Departamento de Quimica OrganicaUniversidad de Cadiz.C/Republica Saharauis/n11510 Puerto Real(Cadiz)Spain

Rice is one of the most exciting and interesting crops in the world both from the social and economic point of *** salinity,quality and other characteristics of the soils in those areas where

关键词： MODIFIED BENZOXAZINOIDS BASED ON ALLELOCHEMICALS FROM GRAMINEAE AS POTENTIAL RICE PROTECTION TOOL AGAINST ECHINOCHLOA CR US-GALLL

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Kinetics and thermodynamic studies of Methyl Orange removal by polyvinylidene fluoride-PEDOT mats

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Journal of Environmental Sciences 2021年第2期33卷 62-73页

作者： Romario J.da Silva Lizeth Carolina Mojica-Sanchez Filipe D.S.Gorza Graciela C.Pedro Bruna G.Maciel Gabriela P.Ratkovski Herica D.daRocha Kamila T.O.do Nascimento Juan CMedina-Llamas Alicia E.Chavez-Guajardo Jose J.Alcaraz-Espinoza Celso P.de Melo Program a de Pos-Graduagao em Ciencia de Materials U niversidade Federal de Pernambuco50670-901 RecifePEBrazil departamento de Ffsica Uniuersidade Federal de Pemambiico50670-901 Reci/ePEBrazil Centro de Estudios Cientificosy Tecnologicos No.18 Instituto Politecnico Nacional98160 ZacatecasZacMexico Unidad Academica de Ciencias de la Tierra Uniuersidad Aut6noma de Zacatecas98058 ZacatecasZacMexico departamento de quimica Universidad Autonoma Metropolitana09340Ciudad de MexicoMexico

We report the preparation of poly(3,4-ethylene dioxythiophene)(PEDOT)-modified polyvinylidene fluoride electrospun fibers and their use as a novel adsorbent material for the removal of the anionic dye Methyl Orange(MO)from aqueous *** novel adsorbent material can be used to selectively remove MO on a wide p H range(3.0-10.0),with a maximum capacity of 143.8 mg/g at p H *** used in a recirculating filtration system,the maximum absorption capacity was reached in a shorter time(20 min)than that observed for batch mode experiments(360 min).Based on the analyses of the kinetics and adsorption isotherm data,one can conclude that the predominant mechanism of interaction between the membrane and the dissolved dye molecules is ***,considering the estimated values for the Gibbs energy,and entropy and enthalpy changes,it was established that the adsorption process is spontaneous and occurs in an endothermic *** good mechanical and environmental stability of these membranes allowed their use in at least 20 consecutive adsorption/desorption cycles,without significant loss of their *** suggest that the physical-chemical characteristics of PEDOT make these hybrid mats a promising adsorbent material for use in water remediation protocols and effluent treatment systems.

关键词： Electrospinning Composite mats Pedot Pvdf mats Dye removal Water remediation

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