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The toxicity test and hypothetical model of Bacillus thuringiensis Cry1Aa helix4

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Science China(Life Sciences) 2002年第6期45卷 561-568页

作者：苏彦辉罗静初朱玉贤曲红张杰 Vachon Vincent Laprade Raynald groupe de recheche en transport membranaire Universite de MontrealMontrealQuebecCanada

Development of targeted biological agents against agricultural insect pests is of prime importance for the elaboration and implementation of integrated pest management strategies that are environment-friendly, respectful of bio-diversity and safer to human health through reduced use of chemical pesticides. A major goal to understand how Bt toxins work is to elucidate the functions of their three domains. Domains II and III are involved in binding specificity and structural integrity, but the function of Domain I remains poorly understood. Using a Manduca sexta BBMV (brush border membrane vesicles) system, we analyzed its responses to Cry1Aa 15 single-point mutations with altered Domain I helix 4 residues. Light scattering assay showed that toxicity was almost lost in 3 mutants, and we observed significantly reduced toxicity in other 7 mutants. However, 5 mutants retained wild-type toxicity. Using computer software, we simulated the three-dimensional structures of helix 4. Both experimental and bioinformatic analysis showed that residues in Cry1Aa Domain I helix 4 were involved in the formation of ion channels that is critical for its insect toxicity.

关键词： Bt toxin proteins, light scattered assay, ion channel, molecular model.

A Multi-Level Selective Maintenance Strategy Combined to Data Mining Approach for Multi-Component System Subject to Propagated Failures

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Journal of Systems Science and Systems Engineering 2022年第3期31卷 313-337页

作者： Mohamed Ali Kammoun Zied Hajej Nidhal Rezg Universite de Lorraine LGIPMF-57000 MetzFrance Ecole poly technique du groupe Honoris United Universities-Tunisia

In several industrial fields like air transport,energy industry and military domain,maintenance actions are carried out during downtimes in order to maintain the reliability and availability of production *** such a circumstance,selective maintenance strategy is considered the reliable solution for selecting the faulty components to achieve the next mission without *** this paper,a novel multi-level decision making approach based on data mining techniques is investigated to determine an optimal selective maintenance *** the first-level,the age acceleration factor and its impact on the component nominal age are used to establish the local *** first decision making employed K-means clustering algorithm that exploited the historical maintenance *** on the first-level intervention plan,the remaining-levels identify the stochastic dependence among components by relying upon Apriori association rules algorithm,which allows to discover of the failure occurrence *** addition,at each decision making level,an optimization model combined to a set of exclusion rules are called to supply the optimal selective maintenance plan within a reasonable time,minimizing the total maintenance cost under a required reliability *** illustrate the robustness of the proposed strategy,numerical examples and a FMS real study case have been solved.

关键词： Selective maintenance stochastic dependence age acceleration factor data mining flexible manufacturing system

Delayed and short course of rapamycin prevents organ rejection after allogeneic liver transplantation in rats

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world Journal of Gastroenterology 2017年第38期23卷 6962-6972页

作者： Salim Hamdani Allan Thiolat Sina Naserian Cynthia Grondin Stéphane Moutereau Anne Hulin Julien Calderaro Philippe Grimbert JoséLaurent Cohen Daniel Azoulay Caroline Pilon Université Paris-Est AP-HP Laboratoire de Biochimie Groupe Hospitalier Henri-Mondor Albert-Chenevier AP-HP Laboratoire de Pharmacologie-Toxicologie Biologiques Groupe Hospitalier Henri-Mondor Albert-Chenevier AP-HP Anatomie et Cytologie Pathologique Groupe Hospitalier Henri-Mondor Albert-Chenevier APHP Service de Chirurgie HPB et Trans-plantation Hépatique Groupe Hospitalier Henri-Mondor Albert-Chenevier Department of Hepato-Pancreato-Biliary Surgery and Liver Transplantation Henri Mondor Hospital

AIMTo test whether a delayed and short course of rapamycin would induce immunosuppressive effects following allogeneic orthotopic liver transplantation (OLT) in *** OLTs were performed using Dark Agouti livers transplanted into Lewis recipients, and syngeneic OLTs were performed using the Lewis rat strain. Rapamycin (1 mg/kg per day) was administered by gavage from day 4 to day 11 post-transplantation. Lymphocyte cellular compartments were analyzed by flow cytometry in draining lymph nodes, non-draining lymph nodes and the spleen at days 11 and 42 in rapamycin-treated rats, untreated control rats and syngeneic grafted rats. Skin grafts from Dark agouti or from F344 RT were performed at day 30 on liver grafted rats treated with *** 8-d course of rapamycin treatment initiated 4 d following transplantation resulted in the survival of grafted rats for more than 100 d. In contrast, untreated rats died of liver failure within 13 to 21 d. The analysis of the cellular compartment revealed an increase in two cellular subpopulations, specifically myeloid-derived suppressor cells (MDSCs) and CD8+CD45RClow T cells, without major modifications in the regulatory T cell (Treg) compartment in treated rats in the early stages after grafting. we evaluated the ability of treated rats to reject third-party allogeneic skin grafts to confirm their immune competence. In contrast, when skin was collected from rats syngeneic to the grafted liver, it was not *** results demonstrate that short and delayed rapamycin treatment allows for tolerance in allogeneic OLT. The results also allowed for the identification of the mechanisms of tolerance induced by rapamycin by identifying MDSCs and CD8+CD45RClow T cells as associated with the state of tolerance.

关键词： Myeloid-derived suppressor cells Liver transplantation Rapamycin Regulatory T cells

Auto-correlation Effects on the sp^3-d Exchange Interaction in Cd1-x MnxTe/CdTe Multilayers

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Chinese Physics Letters 2002年第11期19卷 1679-1682页

作者： K. Afif, A. Benyoussef and J. Diouri Laboratoire de Magnetisme Faculte des Sciences groupe d"Electronique et de Physique du Solide Faculte des Sciences

Band offsets and total energy of wannier excitons in multilayers are affected by dimensional and auto-correlation effects, and sp^3-d coupling is consequently modified. Thus, the calculation of the Zeeman splitting in the quantum wells has to take into account these effects, especially when the mismatch cannot be disregarded. In this work, the theoretical study of these combined effects has been performed for Cd1-xMnxTe/CdTe multilayers embedded by Cd1-yZnyTe as the substrate (or the buffer) on one side and as a cladding layer on the other side. This sample allows us to highlight the role played by the substrate (buffer) and the growth direction on the Zeeman splitting in such multilayers

关键词： Multilayers

Surface Effects on wannier Excitons in Superlattices:Monte Carlo Simulation

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Chinese Physics Letters 2002年第8期19卷 1164-1167页

作者： K. Afif, A. Benyoussef and J. Diouri Laboratoire de Magnetisme Faculte des Sciences groupe d"Electronique et de Physique du Solide Faculte des Science

Using a Monte Carlo quadrature, we calculate by a variational method the binding energy Eb of wannier excitons in N-period m-CdMnTe/n-CdTe superlattices, including or not including the step discontinuity in the potential barrier at the interface, and with varying N as well as well width. The calculation is performed in the framework of the effective mass approximation for both heavy-hole and light-hole excitons. The degree of exciton localization dependence on these parameters is accurately illustrated.

关键词： Monte Carlo methods

Monitoring spatiotemporal soil moisture changes in the subsurface of forest sites using electrical resistivity tomography (ERT)

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Journal of Forestry Research 2022年第5期33卷 1649-1662页

作者： Julian Fäth Julius Kunz Christof Kneisel Institute of Geography and Geology University of WürzburgAm Hubland97074 WürzburgGermany

The effects of drought on tree mortality at forest stands are not completely understood. For assessing their water supply, knowledge of the small-scale distribution of soil moisture as well as its temporal changes is a key issue in an era of climate change. However, traditional methods like taking soil samples or installing data loggers solely collect parameters of a single point or of a small soil volume. Electrical resistivity tomography (ERT) is a suitable method for monitoring soil moisture changes and has rarely been used in forests. This method was applied at two forest sites in Bavaria, Germany to obtain high-resolution data of temporal soil moisture variations. Geoelectrical measurements (2D and 3D) were conducted at both sites over several years (2015-2018/2020) and compared with soil moisture data (matric potential or volumetric water content) for the monitoring plots. The greatest variations in resistivity values that highly correlate with soil moisture data were found in the main rooting zone. Using the ERT data, temporal trends could be tracked in several dimensions, such as the interannual increase in the depth of influence from drought events and their duration, as well as rising resistivity values going along with decreasing soil moisture. The results reveal that resistivity changes are a good proxy for seasonal and interannual soil moisture variations. Therefore, 2D- and 3D-ERT are recommended as comparatively non-laborious methods for small-spatial scale monitoring of soil moisture changes in the main rooting zone and the underlying subsurface of forested sites. Higher spatial and temporal resolution allows a better understanding of the water supply for trees, especially in times of drought.

关键词： Geoelectrical monitoring Forest ecology Hydrology Soil water content

Variant signaling topology at the cancer cell–T-cell interface induced by a two-component T-cell engager

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Cellular & Molecular Immunology 2021年第6期18卷 1568-1570页

作者： Dina Kouhestani Maria Geis Saed Alsouri Thomas GPBumm Hermann Einsele Markus Sauer Gernot Stuhler Department of Internal Medicine II Hematology and OncologyUniversity Clinic WürzburgWürzburgGermany Department of Biotechnology and Biophysics University WürzburgWürzburgGermany

Bispecific T-cell-engaging antibodies(BiTEs)convey antigenic information from cancer to the T-lymphocyte-associated CD3 complex,thus redirecting immune effector cells to induce target cell *** antigenic information from transformed tissues is rarely unambiguous,we recently introduced novel T-cell-engaging antibody fragments called hemibodies for the combinatorial,ultraprecise targeting of cancer.1 Here,we report the unprecedented enhancement of the plasticity of immunological synapse(IS)formation and variant signaling topology at the cancer cell–T-cell interface induced by hemibodies compared to that induced by BiTEs.

关键词： cancer interface topology

Versatile guardians: regenerative regulatory T cells in Parkinson’s disease rodent models

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Signal Transduction and Targeted Therapy 2023年第12期8卷 5578-5580页

作者： Chi wang Ip Jörg wischhusen Department of Neurology University Hospital of WürzburgWürzburgGermany Section for Experimental Tumor Immunology Department of Obstetrics and GynecologyUniversity Hospital of WürzburgWürzburgGermany

In a paper published in Nature,Park and colleagues have explored an innovative approach for improving adoptive neuron transfer,with possible implications for Parkinson´s Disease(PD)and other neurodegenerative diseases.1 By co-transplanting autologous regulatory T(Treg)cells,they managed to reduce surgical trauma-induced neuroinflammation,enhance the safety of cell replacement therapy by inhibiting proliferation of non-dopaminergic cells and improve therapeutic outcomes.

关键词： inflammation inhibiting trauma

A Trojan Horse for live-cell super-resolution microscopy

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Light(Science & Applications) 2020年第1期9卷 1963-1964页

作者： Gerti Beliu Markus Sauer Department of Biotechnology and Biophysics BiocenterUniversity of WürzburgAm Hubland97074 WürzburgGermany

New peptide vehicles enable the efficient live-cell labeling of intracellular organelles with cell-impermeable fluorescent probes by simple coincubation,paving the way for refined multicolor super-resolution fluoresce... 详细信息

关键词： resolution refined enable