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Transcriptomic analysis of Andrias davidianus meat and experimental validation for exploring its bioactive components as functional foods

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Food Science and Human Wellness 2024年第1期13卷 166-172页

作者： Changge Guan Zhenglin Tan Shucheng Li Yi Wang Naoyuki Yamamoto Chong Zhang Songjun Wang Junjie Chen Xinhui Xing Key Laboratory for Industrial Biocatalysis Ministry of Education of ChinaDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China School of Life Science and Technology Tokyo Institute of TechnologyYokohama 226-0026Japan Sensing System research Center National Institute of Advanced Industrial Science and TechnologyTsukuba 305-8565Japan Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China Henan Giant Salamander Protection and Development Association Luoyang 471000China Intelligent Computing research Center Harbin Institute of TechnologyShenzhen 518071China institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518067China

Andrias davidianus(Chinese giant salamander,CGS)is the largest and oldest extant amphibian species in the world and is a source of prospective functional food in ***,the progress of functional peptides mining was slow due to lack of reference genome and protein sequence *** this study,we illustrated full-length transcriptome sequencing to interpret the proteome of CGS meat and obtain 10703 coding DNA *** functional annotation and amino acid composition analysis,we have discovered various genes related to signal transduction,and 16 genes related to *** have also found vast variety of functional peptides through protein coding sequence(CDS)analysis by comparing the data obtained with the functional peptide ***-Pro-Ile predicted by the CDS analysis was released from the CGS meat through enzymatic hydrolysis,suggesting that our approach is *** study suggested that transcriptomic analysis can be used as a reference to guide polypeptide mining in CGS meat,thereby providing a powerful mining strategy for the bioresources with unknown genomic and proteomic sequences.

关键词： Chinese giant salamander Transcriptomic analysis Bioactive components Functional peptides mining

Coordinated Voltage Control of Distribution Network ConsideringMultiple Types of Electric Vehicles

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Energy Engineering 2024年第2期121卷 377-404页

作者： Liang Liu Guangda Xu Yuan Zhao Yi Lu Yu Li Jing Gao State Grid Jibei Electric Power research institute(North China Electric Power research institute Co. Ltd.)Beijing100045China

The couple between the power network and the transportation network(TN)is deepening gradually with the increasing penetration rate of electric vehicles(EV),which also poses a great challenge to the traditional voltage control *** this paper,we propose a coordinated voltage control strategy for the active distribution networks considering multiple types of *** the first stage,the action of on-load tap changer and capacitor banks,etc.,are determined by optimal power flow calculation,and the node electricity price is also determined based on dynamic time-of-use tariff *** the second stage,multiple operating scenarios of multiple types of EVs such as cabs,private cars and buses are considered,and the scheduling results of each EV are solved by building an optimization model based on constraints such as queuing theory,Floyd-Warshall algorithm and traffic flow *** the third stage,the output power of photovoltaic and energy storage systems is fine-tuned in the normal control *** charging power of EVs is also regulated in the emergency control mode to reduce the voltage deviation,and the amount of regulation is calculated based on the fair voltage control mode of ***,we test the modified IEEE 33-bus distribution system coupled with the 24-bus Beijing *** simulation results show that the proposed scheme can mitigate voltage violations well.

关键词： Electric vehicle transportation network voltage control queuing theory

Engineering high amylose and resistant starch in maize by CRISPR/Cas9-mediated editing of starch branching enzymes

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The Crop Journal 2024年第4期12卷 1252-1258页

作者： Mingzheng Ma Shanqiu Sun Jinjie Zhu Xiantao Qi Gaoke Li Jianguang Hu Chuanxiao Xie Changlin Liu State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Hainan Yazhou bay Seed Lab Sanya 572024HainanChina Anhui Agricultural University Hefei 230036AnhuiChina Crops research institute Guangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina

To improve the amylose content(AC)and resistant starch content(RSC)of maize kernel starch,we employed the CRISPR/Cas9 system to create mutants of starch branching enzyme I(SBEI)and starch branching enzyme IIb(SBEIIb).A frameshift mutation in SBEI(E1,a nucleotide insertion in exon 6)led to plants with higher RSC(1.07%),lower hundred-kernel weight(HKW,24.71±0.14 g),and lower plant height(PH,218.50±9.42 cm)compared to the wild type(WT).Like the WT,E1 kernel starch had irregular,polygonal shapes with sharp edges.A frameshift mutation in SBEIIb(E2,a four-nucleotide deletion in exon 8)led to higher AC(53.48%)and higher RSC(26.93%)than that for the WT.E2 kernel starch was significantly different from the WT regarding granule morphology,chain length distribution pattern,X-ray diffraction pattern,and thermal characteristics;the starch granules were more irregular in shape and comprised typical B-type *** SBEI and SBEIIb(E12)had a synergistic effect on RSC,HKW,PH,starch properties,and starch biosynthesis-associated gene ***,SS1,SSIIa,SSIIIa,and SSIIIb were upregulated in E12 endosperm compared to WT *** study lays the foundation for rapidly improving the starch properties of elite maize lines.

关键词： Maize Gene editing Starch branching enzyme I Starch branching enzyme IIb

Theoretical Exploration of Alloying Effects on Stabilities and Mechanical Properties ofγʹPhase in Novel Co–Al–Nb-Base Superalloys

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Acta Metallurgica Sinica(English Letters) 2024年第7期37卷 1238-1248页

作者： Guan-Cheng Gu Zhao-Jing Han Ze-Yu Chen Zhao-Xuan Li Sheng-Bao Xia Zheng-Ning Li Hua Jin Wei-Wei Xu Xing-Jun Liu Shenzhen research institute of Xiamen University School of Aerospace EngineeringXiamen UniversityXiamen361005China institute of Materials Genome and Big Data Harbin Institute of TechnologyShenzhen518055China

The new developedγ/γʹCo–Al–Nb-base alloys show great potentials as high-temperature ***,finding appropriate compositions to improve performance of alloys still poses a great challenge to the development of Co–Al–Nb-base *** by the lack of alloying effects on fundamental properties of criticalγʹphase,we systematically performed a theoretical investigation on the effect of alloying elements TM(TM:Ti,V,Cr,Zr,Mo,Ta,W,Re,and Ru)on phase stabilities and mechanical properties of L1_(2)-typeγʹ(Co,Ni)_(3)(Al,Nb).By analyzing the stability ofγʹphase with respect to its competitive B2 and D0_(19) phases,the results shown that Ti,V,and Cr enhance the L1_(2) stability and widen the L1_(2)–D0_(19) energy barrier,in which V yields the maximum *** analysis of electronic structure indicated that the alternation of valence electrons at fermi level would be the atomic origin for doping TM inγʹ*** calculated results of mechanical properties shown that V and Cr are expected to be optimal dopant for enhancing the strength and the ductility ofγʹ*** addition of Ta is also beneficial for enhancing the strength at the slight expense of ductility ofγʹ*** drawing the mechanical maps,the preferred composition range for the phases with desired properties is roughly demarcated in theory for the multi-addition of V/Cr and V/Ta inγʹ*** findings would be useful for optimizing the performance of novelγ/γʹCo–Al–Nb-base superalloys.

关键词： Co-Al-Nb-base superalloys γ/γʹdual-phase Phase stability Mechanical properties First-principle methods

Production performance analysis of a continental shale oil reservoir in Bohai bay basin

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Petroleum 2024年第2期10卷 294-305页

作者： Quansheng Guan Changwei Chen Xiugang Pu Yonggang Wan Jing Xu Haiwei Zeng Chen Jia Huanhuan Gao Wei Yang Zesen Peng China University of Petroleum Exploration & Development research institute of Dagang Oilfield CNPC Unconventional Petroleum research institute China University of Petroleum

Due to the extremely low permeability of shale formations, the combination of horizontal well and volume fracturing has been proposed as an effective technique to improve the production of Dagang continental shale oil reservoirs. Based on the flow material balance method(FMB) and straight-line analysis(SLA) method, the stimulated reservoir volume(SRV) and drainage volume are determined to identify the flow regimes of the seepage mechanism of shale oil reservoirs. To resolve the challenges of multi-scaled flow regimes and bottom hole pressure(BHP) variation before and after pumping in shale oil wells, a multi-linear analytical flow model was established to predict the future production and the final expected ultimate recoverable oil(EURo) after fitting the historical production dynamics. Based on the results, it can be concluded that the flow regime of a shale oil well in production can be divided into two stages consisting of linear flow within SRV and composite flow from the un-stimulated area to *** effects of fracturing operation parameters, such as fracturing fluid volume and sand/liquid ratio, on shale oil productivity, are analyzed, and insightful suggestions are drawn for the future development of this pay zone.

关键词： Shale oil reservoirs Rate transient analysis Flow material balance Analytical multi-linear model Production performance

Experimental Investigation on Vertical Hydraulic Transport of Ores in Deepsea Mining

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China Ocean Engineering 2024年第1期38卷 42-53页

作者： LIU Lei LIU Jian-cheng LI Xin XU Li-xin ZHANG Xiu-zhan State Key Laboratory of Ocean Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China Sanya Yazhou bay Institution of Deep-sea Sci-Tech Shanghai Jiao Tong UniversitySanya 572000China China Merchants Marine And Offshore research institute Co. Ltd.Shenzhen 518067China China Merchants Deepsea research institute(Sanya)Co. Ltd.Sanya 572000China School of Naval Architecture and Ocean Engineering Jiangsu University of Science and TechnologyZhenjiang 212100China

Deepsea mining has been proposed since the 1960s to alleviate the lack of resources on *** hydraulic transport of collected ores from the seabed to the sea surface is considered the most promising method for industrial *** the present study,an indoor model test of the vertical hydraulic transport of particles was conducted.A noncontact optical method has been proposed to measure the local characteristics of the particles inside a vertical pipe,including the local concentration and particle *** hydraulic gradient of ore transport was evaluated with various particle size distributions,particle densities,feeding concentrations and mixture flow *** transport,the local concentration is larger than the feeding concentration,whereas the particle velocity is less than the mixture *** qualitative effects of the local concentration and local fluid velocity on the particle velocity and slip velocity were *** local fluid velocity contributes significantly to particle velocity and slip velocity,whereas the effect of the local concentration is marginal.A higher feeding concentration and mixture flow velocity result in an increased hydraulic *** effect of the particle size gradation is slight,whereas the particle density plays a crucial role in the transport.

关键词： deepsea mining vertical hydraulic transport experiment flow characteristics

Physiological strategies in wild rodents:immune defenses of commensal rats

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Integrative Zoology 2024年第3期19卷 350-370页

作者： Ivana MIRKOV Dina TUCOVIC Jelena KULAS Anastasija MALESEVIC Dragan KATARANOVSKI Milena KATARANOVSKI Aleksandra POPOV ALEKSANDROV Immunotoxicology Group Department of EcologyInstitute for Biological Research“Sinisa Stankovic”—National Institute of Republic of SerbiaUniversity of BelgradeBelgradeSerbia

The importance of issues associated with urban/commensal rats and mice(property damage,management costs,and health risks)press upon research on these *** the demography of commensal rodents is mostly studied,the need for understanding factors influencing their natural morbidity/mortality is also *** this respect,more attention is expected to be paid to immunity,the physiological mechanism of defense against host survival threats(pathogens,parasites,diseases).Commensal rats and mice carry numerous pathogens that evoke diverse immune *** state of immunity in commensal house mice is studied in great detail,owing to the use of laboratory strains in biomedical *** commensal rats are,compared to mice,carriers of more zoonotic agents,rats’immunity is studied mainly in that *** of these zoonotic agents cause chronic,asymptomatic infections,which justified studies of immunological mechanisms of pathogen tolerance versus clearance regulation in *** of some infections in specific tissues/organs pressed upon analysis of local/regional immune responses and/or immunopathology.A survey of immunological activity/responses in commensal rats is given in this review,with mention of existing data in commensal *** should throw some light on the factors relevant to their morbidity and lifespan,supplementing the knowledge of commensal rodent ecology.

关键词： commensal rats and mice immunopathology infection inflammation tolerance

High-resolution mid-infrared single-photon upconversion ranging

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Photonics research 2024年第6期12卷 1294-1302页

作者： SHUHONG JIANG KUN HUANG TINGTING YU JIANAN FANG BEN SUN YAN LIANG QIANG HAO E.WU MING YAN HEPING ZENG State Key Laboratory of Precision Spectroscopy East China Normal UniversityShanghai 200062China Chongqing Key Laboratory of Precision Optics Chongqing Institute of East China Normal UniversityChongqing 401121China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China School of Optical Electrical and Computer Engineering University of Shanghai for Science and TechnologyShanghai 200093China Shanghai research Center for Quantum Sciences Shanghai 201315China Guangyang bay Laboratory Chongqing Institute for Brain and IntelligenceChongqing 400064China

Single-photon laser ranging has widespread applications in remote sensing and target ***,highly sensitive light detection and ranging(lidar)has long been restricted in the visible or near-infrared *** appealing quest is to extend the operation wavelength into the mid-infrared(MIR)region,which calls for an infrared photon-counting system at high detection sensitivity and precise temporal ***,we devise and demonstrate an MIR upconversion lidar based on nonlinear asynchronous optical ***,the infrared probe is interrogated in a nonlinear crystal by a train of pump pulses at a slightly different repetition rate,which favors temporal optical scanning at a picosecond timing resolution and a kilohertz refreshing rate ***,the cross-correlation upconversion trace is temporally stretched by a factor of 2×10^(4),which can thus be recorded by a low-bandwidth silicon *** combination with the time-correlated photon-counting technique,the achieved effective resolution is about two orders of magnitude better than the timing jitter of the detector itself,which facilitates a ranging precision of 4μm under a low detected flux of 8×10^(−5) photons per *** presented MIR time-of-flight range finder is featured with single-photon sensitivity and high positioning resolution,which would be particularly useful in infrared sensing and imaging in photon-starved scenarios.

关键词： resolution lidar repetition

Origin of condensate oil and natural gas in Bozhong 19-6 gas field,Bohai bay Basin

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Energy Geoscience 2024年第1期5卷 54-62页

作者： Jianyong Xu Wei Li CNOOC research institute Co.Ltd. Beijing100028China

The discovery of the Bozhong 19-6 gas field has opened a new frontier for deep gas exploration in the Bohai bay Basin,with a great potential for further gas ***,poor understanding of oil and gas origin has been limiting the exploration progress in this *** clarify the origin of condensate oil and gas in Bozhong 19-6 gas field,this study adequately utilized the organic geochemical analysis data to investigate the composition and geochemical characteristics of condensate oil and natural gas,and analyzed the relationship between condensate oil and the three sets of source rocks in the nearby *** show that the lighter components dominate the condensate oil,with a forward type *** parent material of crude oil was primarily deposited in a shallow,clay-rich,low-salinity,weakly reducing aquatic *** condensate and natural gas have similar parent source characteristics and maturity,with Ro ranging from 1.4%to 1.6%.Both are products of high maturity stage,indicating that they are hydrocarbon compounds produced by the same group of source rocks in the same ***-sources correlation shows that condensate oil and gas mainly originate from the source rocks of the third member of Shahejie Formation in the nearby subsags of the Bozhong 19-6 structural belt.

关键词： Bozhong Sag Natural gas Condensate oil Light hydrocarbon Gas-source correlation Oil-source correlation