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Insect science 2024年第1期31卷 201-210页

作者： Eisuke Tasaki Yorihiro Yamamoto Yoshihito Iuchi department of biological Chemistry Graduate School of Sciences and Technology for InnovationYamaguchi UniversityYamaguchiJapan School of Bioscience and Biotechnology Tokyo University of TechnologyHachiojiTokyoJapan

Termite queens and kings live longer than nonreproductive *** molecular mechanisms contributing to their long lifespan have been investigated;however,the underlying biochemical explanation remains *** Q(CoQ),a component of the mitochondrial electron transport chain,plays an essential role in the lipophilic antioxidant defense *** beneficial effects on health and longevity have been well studied in several ***,we demonstrated that long-lived termite queens have significantly higher levels of the lipophilic antioxidant CoQ_(10) than *** chromatography analysis revealed that the levels of the reduced form of CoQ_(10) were 4 fold higher in the queen's body than in the worker's *** addition,queens showed 7 fold higher levels of vitamin E,which plays a role in antilipid peroxidation along with CoQ,than ***,the oral administration of CoQ_(10) to termites increased the CoQ_(10) redox state in the body and their survival rate under oxidative *** findings suggest that CoQ_(10) acts as an efficient lipophilic antioxidant along with vitamin E in long-lived termite *** study provides essential biochemical and evolutionary insights into the relationship between CoQ_(10) concentrations and termite lifespan extension.

关键词： antioxidant defense system coenzyme Q termite queens vitamin E

Highly efficient vanadium redox flow batteries enabled by a trilayer polybenzimidazole membrane assembly

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Carbon Energy 2024年第7期6卷 191-204页

作者： Trung Tuyen Bui Mingyu Shin Mohammad Rahimi Anders Bentien Yongchai Kwon Dirk Henkensmeier Hydrogen·Fuel Cell Research Center Korea Institute of Science and Technology(KIST)SeoulRepublic of Korea department of Chemical and Biomolecular Engineering Seoul National University of Science and TechnologySeoulRepublic of Korea department of biological and Chemical Engineering Aarhus UniversityAarhusDenmark KIST School Division of Energy and Environment TechnologyUniversity of Science and TechnologySeoulRepublic of Korea graduate School of Energy and Environment Korea UniversitySeoulRepublic of Korea

A novel polybenzimidazole(PBI)-based trilayer membrane assembly is developed for application in vanadium redox flow battery(VRFB).The membrane comprises a 1μm thin cross-linked poly[2,2′-(p-oxydiphenylene)−5,5′-bibenzimidazole](OPBI)sandwiched between two 20μm thick porous OPBI membranes(p-OPBI)without further lamination *** trilayer membrane demonstrates exceptional properties,such as high conductivity and low area-specific resistance(ASR)of 51 mS cm^(−1) and 81mΩ cm^(2),*** with vanadium electrolyte increases the ASR of trilayer membrane only to 158mΩ cm^(2),while that of Nafion is 193mΩ cm^(2).VO^(2+) permeability is 2.73×10^(-9) cm^(2) min^(−1),about 150 times lower than that of Nafion *** addition,the membrane has high mechanical strength and high chemical stability against VO^(2+).In VRFB,the combination of low resistance and low vanadium permeability results in excellent performance,revealing high Coulombic efficiency(>99%),high energy efficiency(EE;90.8% at current density of 80mA cm^(−2)),and long-term *** EE is one of the best reported to date.

关键词： polybenzimidazole porous membrane proton conductivity trilayer VRFBs

Synthesis and electrochemical performance of Li_3V_2(PO_4)_3 by optimized sol-gel synthesis routine

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Transactions of Nonferrous Metals Society of China 2010年第8期20卷 1545-1549页

作者：张倩李艳红钟胜奎肖新和颜波 department of biological and Chemical Engineering Guangxi University of Technology Guangxi Key Laboratory of Environmental Engineering Protection and AssessmentCollege of Environmental Science and EngineeringGuilin University of Technology department of Material and Chemical Engineering Guilin University of Technology Hunan Institute of Humanities Science and Technology

Li3V2（PO4）3 samples were synthesized by sol-gel route and high temperature solid-state reaction. The influence of Li3V2（PO4）3 as cathode materials for lithium-ion batteries on electrochemical performances was investigated. The structure of Li3Va（PO4）3 as cathode materials for lithium-ion batteries and morphology of Li3V2（PO4）3 were characterized by X-ray diffractometry （XRD） and scanning electron microscopy （SEM）. Electrochemical performances were characterized by charge/discharge and AC impedance measurements. Li3V2（PO4）3 with smaller grain size shows better performances in terms of the discharge capacity and cycle stability. The improved electrochemical properties of Li3V2（PO4）3 are attributed to the refined grains and enhanced electrical conductivity. AC impedance measurements also show that the Li3V2（PO4）3 synthesized by sol-gel route exhibits significantly decreased charge-transfer resistance and shortened migration distance of lithium ions.

关键词： lithium ion batteries cathode material Li3V2(PO4)3 sol-gel method

A simple fabricated thickness-based stiffness gradient for cell studies

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science Bulletin 2017年第3期62卷 222-228页

作者： Yiwei Shu HO Nam Chan Dongshi Guan Hongkai Wu Lan Ma Division of Life science & Health Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China department of Chemistry The Hong Kong University of Science and Technology Hong Kong China department of Physics The Hong Kong University of Science and Technology Hong Kong China

In this work, we developed a simple method to fabricate a thickness-based continuous stiffness gradient for biological studies. It was made by glass slides, polydimethylsiloxane （PDMS） pre-polymer, spacer and clips only, without any sophisticated equipment. It is easy to fabricate in any general biological and pharmaceutical laboratories. The stiffness gradient was characterized in terms of apparent Young＇s modulus by atomic force microscopy （AFM） and the Young＇s modulus along the gradient was found to be 8.5- 120 kPa, which is within the physiological relevant range. HeLa-C3 ceils were cultured on the gradient to study their morphological behavior according to the substrate stiffness. Furthermore, the drug efficiency of etoposide, an anti-cancer drug, was studied along the substrate stiffness gradient. It was found that HeLa-C3 cells cultured on the soft region of the gradient （8.5-11 kPa） are more sensitive to etoposide. We believe the proposed device could promote cell investigations and drug screenings on a substrate with comparable stiffness to the native tissue.

关键词： Stiffness gradient Cell analysis PDMS Etoposide

biological properties of self-assembled nanofibers of elastin-like block polypeptides for tissue-engineered vascular grafts:platelet inhibition,endothelial cell activation and smooth muscle cell maintenance

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Regenerative Biomaterials 2023年第1期10卷 386-395页

作者： Kazuki Natsume Jin Nakamura Kazuhide Sato Chikara Ohtsuki Ayae Sugawara-Narutaki department of Materials Chemistry Graduate School of EngineeringNagoya UniversityNagoya 464-8603Japan department of biological Functions Engineering Graduate School of Life Science and Systems EngineeringKyushu Institute of TechnologyFukuoka 808-0196Japan Institute for Advanced Research Nagoya UniversityNagoya 464-8601Japan department of Respiratory Medicine Graduate School of MedicineNagoya UniversityNagoya 466-8560Japan department of Energy Engineering Graduate School of EngineeringNagoya UniversityNagoya 464-8603Japan

Strategic materials design is essential for the development of small-diameter,tissue-engineered vascular ***-assembled nanofibers of elastin-like polypeptides represent promising vascular graft components as they replicate the organized elastin structure of native blood ***,the bioactivity of nanofibers can be modified by the addition of functional peptide *** the present study,we describe the development of a novel nanofiber-forming elastin-like polypeptide(ELP)with an arginine-glutamic acid-aspartic acid-valine(REDV)*** biological characteristics of the REDV-modified ELP nanofibers relevant to applications in vascular grafting were compared to ELP without ligands for integrin,ELP with arginine-glycine-aspartic acid(RGD)sequence,collagen and cell culture *** them,REDV-modified ELP nanofibers met the preferred biological properties for vascular graft materials,i.e.(i)inhibition of platelet adhesion and activation,(ii)endothelial cell adhesion and proliferation and(iii)maintenance of smooth muscle cells in a contractile phenotype to prevent cell *** results indicate that REDV-modified ELP nanofibers represent promising candidates for the further development of small-diameter vascular grafts.

关键词： elastin-like polypeptide nanofiber platelet endothelial cell smooth muscle cell

Fluorescence imaging of drug target proteins using chemical probes

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Journal of Pharmaceutical Analysis 2020年第5期10卷 426-433页

作者： Hao Zhu Itaru Hamachi department of Synthetic Chemistry and biological Chemistry Graduate School of EngineeringKyoto UniversityKatsuraNishikyo-kuKyoto615-8510Japan ERATO Innovative Molecular technology for Neuroscience Project Japan Science and Technology Agency(JST)Kyoto615-8530Japan

Fluorescence imaging can provide valuable information on the expression,distribution,and activity of drug target *** probes are useful small-molecule tools for fluorescence imaging with high structural flexibility and *** this review,we briefly introduce two classes of fluorescent probes for the visualization of drug target *** activatable probes make use of the specific enzymatic transformations that generally produce a fluorogenic response upon reacting with target ***,specific imaging can be conferred with a ligand that drives the probes to target proteins,where the labeling relies on noncovalent binding,covalent inhibition,or traceless labeling by ligand-directed chemistry.

关键词： Fluorescence imaging Drug target Chemical probe Ligand-directed chemistry

Production and Accumulation of Xylooligosaccharides with Long Chains by Growing Culture and Xylanase of a Mutant Strain of Bacillus pumilus X-6-19

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生物工程学报 2008年第7期24卷 1221-1227页

作者： Qingzhu Yuan Tsuyoshi Adachi Shinji Takenaka Shuichiro Murakami Machiko Tanaka Kenji Aoki Division of Life science Graduate School of Science and Technology Kobe University Rokko Kobe 657-8501 Japan department of Applied biological Chemistry Graduate school of Agricultural Science Kobe University Rokko Kobe 657-8501 Japan Research ＆ Development department Bunsen Co. Ltd. Shingu-cho Tatsuno 679-4393 Japan

Bacillus pumilus X-6-9 isolated from soil and subsequently identified, produced xylooligosaccharides with long chains from xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increase in the production of the xylooligosaccharides was established, when compared to the original culture conditions of B. pumilus X-6-19. The addition of D-glucose to the culture of the mutant strain U-3 of B. pumilus X-6-9 repressed the synthesis of β-xylosidase, but not xylanase. Thus, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharides with long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized. The hydrolyzates generated by the purified xylanase contained xylobiose, xylotriose, xylotetraose, and xylopentaose, but not xylose.

关键词：木聚糖酶菌株细菌堆积作用

Biphasic Nano-Domains of Planar Lipid Bilayer Complexed with Fluorogenic Polymer Reporter Tailored for Antimicrobial Detection

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CCS Chemistry 2022年第11期4卷 3637-3647页

作者： Jimin Hwang Woo Hyuk Jung Yeol Kyo Choi Dong June Ahn department of Chemical and biological Engineering Korea UniversitySeoul 02841 department of biological sciences Lehigh UniversityBethlehemPennsylvania 18015 KU-KIST graduate School of Converging science and technology Korea UniversitySeoul 02841

We have fabricated an unexpected type of supported planar bilayer composed of receptor phospholipids and single-chained diacetylenes as fluorogenic reporters using protruded anchor moieties with a positive terminal *** topographical and surface thermodynamic analyses,as well as molecular dynamics simulations,revealed the coexistence of well-dispersed liquid-condensed(L_(c))domains forming nano-islands and liquid-expanded(L_(e))region in the planar bilayer,enhancing sensitivity against a prototype of ubiquitous membrane-associated antimicrobial peptides,*** L_(e)regions,acting as target receptors,enabled sensitive detection as the melittin adsorbed and inserted into these regions due to strong hydrophobic interactions between phospholipids and *** L_(c)domains,serving as signal reporters,enabled diacetylenes to assemble,polymerize,and fluoresce in response to the insertion of melittin into the L_(e)***,biphasic nanodomains of the planar lipid bilayer finally endowed this sensor system with a detection range of 100μMto 50 nM and a limit of detection(LOD)of∼37 nM for *** exceeded the operational performance of the colorimetric polydiacetylene vesicle solution 45 times,which reportedly ranged from 100 to 4μM with an LOD of∼1.7μM.

关键词： biphasic nano-domain supported lipid bilayer fluorogenic polymer reporter liquid-expanded region liquid-condensed domain

Real-time 3D Microtubule Gliding Simulation Accelerated by GPU Computing

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International Journal of Automation and computing 2016年第2期13卷 108-116页

作者： Gregory Gutmann Daisuke Inoue Akira Kakugo Akihiko Konagaya department of Computational Intelligence and Systems science Tokyo Institute of Technology Faculty of science Hokkaido University graduate School of Chemical sciences and Engineering Hokkaido University

A microtubule gliding assay is a biological experiment observing the dynamics of microtubules driven by motor proteins fixed on a glass surface. When appropriate microtubule interactions are set up on gliding assay experiments, microtubules often organize and create higher-level dynamics such as ring and bundle structures. In order to reproduce such higher-level dynamics on computers, we have been focusing on making a real-time 3D microtubule simulation. This real-time 3D microtubule simulation enables us to gain more knowledge on microtubule dynamics and their swarm movements by means of adjusting simulation paranleters in a real-time fashion. One of the technical challenges when creating a real-time 3D simulation is balancing the 3D rendering and the computing performance. Graphics processor unit （GPU） programming plays an essential role in balancing the millions of tasks, and makes this real-time 3D simulation possible. By the use of general-purpose computing on graphics processing units （GPGPU） programming we are able to run the simulation in a massively parallel fashion, even when dealing with more complex interactions between microtubules such as overriding and snuggling. Due to performance being an important factor, a performance n, odel has also been constructed from the analysis of the microtubule simulation and it is consistent with the performance measurements on different GPGPU architectures with regards to the number of cores and clock cycles.

关键词： Microtubule gliding assay 3D computer graphics and simulation parallel computing performance analysis general- purpose computing on graphics processing units (GPGPU) compute unified device arshitecture (CUDA) DirectX.

Roles of computational modelling in understanding p53 structure, biology, and its therapeutic targeting

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Journal of Molecular Cell Biology 2019年第4期11卷 306-316页

作者： Yaw Sing Tan Yasmina Mhoumadi Chandra S. Verma Bioinformatics Institute Agency for Science Technology and Research Singapore 138671 School of biological sciences Nanyang Technological University Singapore 637551 department of biological sciences National University of Singapore Singapore 117543

The transcription factor p53 plays pivotal roles in numerous biological processes, including the suppression of tumours. The rich availability of biophysical data aimed at understanding its structure–function relationships since the 1990s has enabled the application of a variety of computational modelling techniques towards the establishment of mechanistic models. Together they have provided deep insights into the structure, mechanics, energetics, and dynamics of p53. In parallel, the observation that mutations in p53 or changes in its associated pathways characterize several human cancers has resulted in a race to develop therapeutic modulators of p53, some of which have entered clinical trials. This review describes how computational modelling has played key roles in understanding structural-dynamic aspects of p53, formulating hypotheses about domains that are beyond current experimental investigations, and the development of therapeutic molecules that target the p53 pathway.

关键词： p53 structure computational modelling therapeutic targeting