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Nano-sized Fe_2O_3/Fe_3O_4 facilitate anaerobic transformation of hexavalent chromium in soil–water systems

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Journal of Environmental Sciences 2017年第7期29卷 329-337页

作者： Yaxian Zhang Hua Li Libo Gong Guowen Dong Liang Shen Yuanpeng Wang Qingbiao Li Department of chemical and biochemical Engineering College of Chemistry and Chemical EngineeringXiamen University College of Chemistry and Life Science Quanzhou Normal University

The purpose of this study is to investigate the effects of nano-sized or submicro Fe_2O_3/Fe_3O_4 on the bioreduction of hexavalent chromium（Cr（VI）） and to evaluate the effects of nano-sized Fe_2O_3/Fe_3O_4 on the microbial communities from the anaerobic flooding *** results indicated that the net decreases upon Cr（VI） concentration from biotic soil samples amended with nano-sized Fe_2O_3（317.1 ± 2.1 mg/L） and Fe_3O_4（324.0 ± 22.2 mg/L） within21 days,which were approximately 2-fold of Cr（VI） concentration released from blank control assays（117.1 ± 5.6 mg/L）.Furthermore,the results of denaturing gradient gel electrophoresis（DGGE） and high-throughput sequencing indicated a greater variety of microbes within the microbial community in amendments with nano-sized Fe_2O_3/Fe_3O_4 than the control ***,Proteobacteria occupied a predominant status on the phylum level within the indigenous microbial communities from chromium-contaminated ***,some partial decrease of soluble Cr（VI） in abiotic nano-sized Fe_2O_3/Fe_3O_4 amendments was responsible for the adsorption of nano-sized Fe_2O_3/Fe_3O_4 to soluble Cr（VI）.Hence,the presence of nano-sized Fe_2O_3/Fe_3O_4 could largely facilitate the mobilization and biotransformation of Cr（VI） from flooding soils by adsorption and bio-mediated processes.

关键词： Chromium Iron oxide Nanoparticle Reduction High-throughput sequencing Cr(Ⅵ) reducing bacteria

Multicolor carbon dots with concentration-tunable fluorescence and solvent-affected aggregation states for white light-emitting diodes

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Nano Research 2020年第1期13卷 52-60页

作者： Fanyong Yan Yingxia Jiang Xiaodong Sun Junfu Wei Liang Chen Yuyang Zhang State Key Laboratory of Separation Membranes and Membrane processes School of Chemistry and Chemical EngineeringTiangong UniversityTianjin300387China graduate School of Life Science Hokkaido UniversitySapporo0010024Japan

Multicolor emissive carbon dots(M-CDs)have tremendous potential applications in manifold fields of bioimaging,biomedicine and light-emitting *** now,it is still difficult to produce fluorescence tunable CDs with high quantum yield across the entire visible *** this work,a type of M-CDs with concentration-tunable fluorescence and solvent-affected aggregation states was synthesized by solvothermal treatment of citric acid(CA)and 1-(2-pyridylazo)-2-naphthol(PAN)and the formation mechanism was monitored by different reaction time and raw material *** fluorescence spectra of M-CDs in organic solvents can range from 350 to 750 nm by adjusting the concentration.M-CDs possess different aggregation states in water and organic solvents,accompanied by different fluorescence emission,which is attributed to the different surface states of various component CDs in ***,the obtained products can be uniformly dispersed into polymethylmethacrylate(PMMA)solutions as well as epoxy resins to fabricate transparent CDs/PMMA films and CDs/epoxy composites,which can effectively prevent the aggregation and produce multicolor and white light-emitting diodes(WLED).In addition,the prepared WLED with Commission Internationale de L’Eclairage(CIE)of(0.29,0.31)by using M-CDs/epoxy resin as packages,demonstrating the M-CDs exhibit potential applications for light-emitting devices.

关键词： carbon dots formation mechanism concentration-tunable fluorescence solvent-affected aggregation states light-emitting devices

TMPRSS2 and SARS-CoV-2 SPIKE interaction assay for uHTS

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Journal of Molecular Cell Biology 2023年第3期15卷 32-43页

作者： Danielle Cicka Qiankun Niu Min Qui Kun Qian Eric Miller Dacheng Fan Xiulei Mo Andrey AIvanov Stefan GSarafianos Yuhong Du Haian Fu Department of Pharmacology and chemical Biology Emory University School of MedicineAtlantaGA 30322USA graduate program in Molecular and Systems Pharmacology Laney Graduate School of Emory UniversityAtlantaGA 30322USA Emory chemical Biology Discovery Center Emory University School of MedicineAtlantaGA 30322USA Winship Cancer Institute of Emory University AtlantaGA 30322USA Laboratory of biochemical Pharmacology Department of PediatricsEmory University School of MedicineAtlantaGA 30322USA

SARS-CoV-2,the coronavirus that causes the disease COVID-19,has claimed millions of lives over the past 2 *** demands rapid development of effective therapeutic agents that target various phases of the viral replication *** interaction between host transmembrane serine protease 2(TMPRSS2)and viral SPIKE protein is an important initial step in SARS-CoV-2 infection,offering an opportunity for therapeutic development of viral entry ***,we report the development of a time-resolved fluorescence/Förster resonance energy transfer(TR-FRET)assay for monitoring the TMPRSS2–SPIKE interaction in lysate from cells co-expressing these *** assay was configured in a 384-well-plate format for high-throughput screening with robust assay *** enable large-scale compound screening,we further miniaturized the assay into 1536-well ultrahigh-throughput screening(uHTS)format.A pilot screen demonstrated the utilization of the assay for *** optimized TR-FRET uHTS assay provides an enabling platform for expanded screening campaigns to discover new classes of small-molecule inhibitors that target the SPIKE and TMPRSS2 protein–protein interaction.

关键词： SARS-CoV-2 COVID-19 protein-protein interaction TMPRSS2 SPIKE high-throughput screening TR-FRET

Design and regulation of the surface and interfacial behavior of protein molecules

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Chinese Journal of chemical Engineering 2020年第11期28卷 2837-2847页

作者： Qianqian Hou Nanxing Li Yuanyuan Chao Shihao Li Lin Zhang Department of biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China

Surface and interfacial behavior of protein molecules are crucial for the protein function involved in many biochemical processes and biomedical products such as enzyme design,bio-separation,drug design and *** article is devoted to an overview of design and regulation of the surface and interfacial behavior of protein *** improvement of enzyme surface such as the directed evolution and the rational design of enzymes is introduced at first,followed by the rational design of protein interface for the protein ***,the design of micro-environment and ligands are described as two examples for the design guided by protein *** the design of protein surface and interface with the help of artificial intelligence will be discussed.

关键词： Protein Enzyme Surface Interface Design Artificial intelligence

Semi-synthesis of disulfide-linked branched tri-ubiquitin mimics

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Science China Chemistry 2018年第4期61卷 412-417页

作者： Yanyan Si Lujun Liang Shan Tang Yunkun Qi Yong Huang Lei Liu Key Laboratory of chemical Genomics School of Chemical Biology and BiotechnologyPeking UniversityShenzhen Graduate School Department of Chemistry Tsinghua University

Ubiquitin(Ub) chain isopeptide bond mimics are useful molecules for biochemical and biophysical studies. Herein, we report the semi-synthesis of the disulfide-linked K11/K48-branched tri-Ub(Ub_3^(11/48)(S-S)), the first example of an isopeptide mimic for the branched Ub chains,which have recently emerged as an interesting category of Ub modifications. Our strategy comprised the El-dependent synthesis of the Ub conjugate of aminoethanethiol, followed by disulfide formation with Ub(K11 C, K48 C). The structure of the synthetic isopeptide bond mimics was verified by the crystal structure of Ub_3^(11/48)(S-S). Deubiquitination and pulldown assays indicated that the synthetic Ub_3^(11/48)(S-S) could be hydrolyzed by linkage-specific deubiquitinases(K11-specific Cezanne and K48-specific OTUB1), and recognized by proteasomal ubiquitin receptor S5 a.

关键词： ubiquitin chain isopeptide bond mimics disulfide-directed strategy branched Ub chains crystal structure

R&D Landscape for Breast Cancer through Patent Documents

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Journal of Cancer Therapy 2013年第7期4卷 56-64页

作者： Adelaide M. S. Antunes Iolanda M. Fierro Rafaela Di Sabato Guerrante Priscila Rohem dos Santos Flavia Maria Lins Mendes Federal University of Rio de Janeiro Postgraduate Program in Technology of Chemical & Biochemical Processes Rio de Janeiro Brazil National Institute of Industrial Property Rio de Janeiro Brazil

Cancer is a leading cause of death worldwide, and is estimated to be the cause of 13.1 million deaths in 2030. Breast cancer is the second cancer in the global mortality ranking, considering both sexes. Due to the burden of breast cancer worldwide, this paper aims to present an overview of the main R&D efforts focusing on breast cancer treatment. Patents were retrieved from the Derwent Innovations Index?, which has a specific code for pharmaceuticals related to breast cancer. A total of 423 patent documents filed in recent years were identified, of which 126 are exclusively for breast cancer, 169 for breast cancer and other cancers, and 128 are inventions related to the treatment of cancer and other diseases. The patent documents were classified into two large groups, the first of which had a predominance of claims for antibodies, proteins and polypeptides for use in medication production, while the second focuses on gene therapy, nucleotides and RNA. The country with the majority of priority patent applications was found to be the United States, followed by China and Japan.

关键词： Patent Breast Cancer Treatment R&D Trends

Nenofiltration for separation and purification of saccharides from biomass

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Frontiers of chemical Science and Engineering 2021年第4期15卷 837-853页

作者： Xianhui Li Sheng Tan Jianquan Luo Manuel Pinelo Process and Systems Engineering Center(PROSYS) Department of Chemical and Biochemical EngineeringTechnical University of DenmarkDK-2800 LyngbyDenmark State Key Laboratory of biochemical Engineering Institute of Process EngineeringUniversity of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100190China

Saccharide production is critical to the development of biotechnology in the field of food and *** extraction of saccharide from biomass-based hydrolysate mixtures has become a trend due to low cost and abundant biomass *** to conventional methods of fractionation and recovery of saccharides,nanofiltration(NF)has received considerable attention in recent decades because of its high selectivity and low energy consumption and environmental *** this review the advantages and challenges of NF based technology in the separation of saccharides are critically *** membrane processes,i.e.,combining NF with ultrafiltration,can complement each other to provide an efficient approach for removal of unwanted solutes to obtain higher purity ***,use of NF membrane separation technology is limited due to irreversible membrane fouling that results in high capital and operating *** development of NF membrane technology should therefore focus on improving material stability,antifouling ability and saccharide targeting selectivity,as well as on engineering aspects such as process optimisation and membrane module design.

关键词： saccharides nanofiltration membrane hybrid membrane process biomas

Single-molecule biotechnology for protein researches

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Chinese Journal of chemical Engineering 2021年第2期34卷 212-224页

作者： Xiaoyan Zhuang Qian Wu Aihui Zhang Langxing Liao Baishan Fang Department of chemical and biochemical Engineering College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China The Key Lab for Synthetic Biotechnology of Xiamen City Xiamen UniversityXiamen 361005China The Key Laboratory for chemical Biology of Fujian Province Xiamen UniversityXiamen 361005China

Cells employ proteins to perform metabolic functions and maintain active physiological state through charge transfer and energy *** processes are carried out in a narrow space precisely and rapidly,which,no doubt,bring great difficulty for their detection and ***,in recent years,the development and expansion of single-molecule technique in protein research make monitoring the dynamical changes of protein at single-molecule level a reality,which also provides a powerful tool for the further exploration of new phenomena and new mechanisms of life *** paper aims to summarize the working principle and essential achievements of single-molecule technique in protein research in recent five *** focus on not only dissecting the difference of nanopores,atomic force microscope,scanning tunneling microscope,and optical tweezers technique,but also discussing the great significance of these single-molecule techniques in investigating intramolecular and intermolecular interactions,electron transport,and conformational ***,the opportunities and challenges of the single-molecule technique in protein research are discussed,which provide a new door for single-molecule protein research.

关键词： Single-molecule protein Enzyme Nanopore Atomic force microscope Scanning tunneling microscope Optical tweezers Biotechnology Imaging

Photocatalytic degradation of aqueous Methyl Orange using nitrogen-doped TiO_2 photocatalyst prepared by novel method of ultraviolet-assisted thermal synthesis

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Journal of Environmental Sciences 2018年第4期30卷 81-93页

作者： Mohsen Nasirian Mehrab Mehrvar graduate programs in Environmental Applied Science and Management Ryerson University Department of chemical Engineering Ryerson University

A nitrogen-doped titanium dioxide composite photocatalyst（N–TiO2） with heterojunction structures is synthesized by three different approaches： a novel UV-assisted thermal synthesis, annealing, and microwave technique. Photocatalytic activities of synthesized photocatalysts are evaluated by the degradation of Methyl Orange under ultraviolet light types A（UV-A）, B（UV-B）, and C（UV-C）, visible light, and direct sunlight irradiation. Results show that by using N–TiO_2 photocatalyst prepared by the UV-assisted thermal synthesis and annealing, the degradation increases by 16.5% and 20.4%, respectively, compared to that by bare TiO2. The best results are obtained at a nitrogen to TiO2 mass ratio of 0.15（N：TiO2）. The enhancement of the photocatalytic activity observed in the visible range is mainly attributed to the increasing separation rate of photogenerated charge carriers. The novel UV-assisted thermal synthesis has produced encouraging results as a preparation method for the nitrogen-doped TiO2 photocatalyst; thus, further studies are recommended for process optimization, immobilization, and scale-up to evaluate its applicability in wastewater treatment.

关键词： Ultraviolet-assisted thermal synthesis N–TiO2 Photocatalysis Advanced oxidation processes