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Short-term environmental enrichment exposure induces proliferation and maturation of doublecortin-positive cells in the prefrontal cortex

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Neural Regeneration Research 2014年第3期9卷 318-328页

作者： Chunling Fan Mengqi Zhang Lei Shang Ngobe Akume Cynthia Zhi Li Zhenyu Yang Dan Chen Jufang Huang Kun Xiong Department of Anatomy and Neurobiology Central South University School of Basic Medical Sciences grade 2006 Eight-year Medicine Doctor Program Central South University Xiangya School of Medicine grade 2011 Six-year Medicine Program of International Student Central South University Xiangya School of Medicine grade 2008 Eight-year Medicine Doctor Program Central South University Xiangya School of Medicine

Previous studies have demonstrated that doublecortin-positive immature neurons exist pre- dominantly in the superficial layer of the cerebral cortex of adult mammals such as guinea pigs, and these neurons exhibit very weak properties of self-proliferation during adulthood under physiological conditions. To verify whether environmental enrichment has an impact on the proliferation and maturation of these immature neurons in the prefrontal cortex of adult guinea pigs, healthy adult guinea pigs were subjected to short-term environmental enrichment. Animals were allowed to play with various cognitive and physical stimulating objects over a period of 2 weeks, twice per day, for 60 minutes each. Immunofluorescence staining results indicated that the number of doublecortin-positive cells in layer II of the prefrontal cortex was significantly increased after short-term environmental enrichment exposure. In addition, these doublecortin-positive cells co-expressed 5-bromo-2-deoxyuridine （a marker of cell prolifera- tion）, c-Fos （a marker of cell viability） and NeuN （a marker of mature neurons）. Experimental findings showed that short-term environmental enrichment can induce proliferation, activation and maturation of doublecortin-positive cells in layer II of the prefrontal cortex of adult guinea pigs.

关键词： nerve regeneration neurogenesis prefrontal cortex neocortex guinea pig doublecortinprotein cell proliferation neurons 5-bromodeoxyuridine NSFC grant neural regeneration

Pine pollen inhibits cell apoptosis-related protein expression in the cerebral cortex of mice with arsenic poisoning

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Neural Regeneration Research 2012年第12期7卷 896-899页

作者： Yanhong Luo Yaodong Wei Taizhong Wang Dongzhu Chen Tiansheng Lu Ruibo Wu Keke Si Department of Clinical Biochemistry and Molecular Biology Youjiang Medical College for Nationafities Baise 533000 Guangxi Zhuang Autonomous Region China Department of Biochemistry and Molecular Biology Youjiang Medical College for Nationalities Baise 533000 Guangxi Zhuang Autonomous Region China grade 2008 Department of Medical Laboratory Science Youjiang Medical College for Nationalities Baise 533000 Guangxi Zhuang Autonomous Region China

Previous studies have demonstrated that pine pollen can inhibit cerebral cortical cell apoptosis in mice with arsenic poisoning. The present study sought to detect the influence of pine pollen on apoptosis-related proteins. Immunohistochemistry, western blotting and enzyme-linked immunosorbent assays were used to measure the levels of apoptosis-related proteins in the cerebral cortex of mice with arsenic poisoning. Results indicated that pine pollen suppressed cell apoptosis in the cerebral cortex of arsenic-poisoned mice by reducing Bax, Bcl-2 protein expression and increasing p53 protein expression.

关键词： pine pollen arsenic poisoning apoptosis cerebral cortex neural regeneration

Effects of Gingko biloba leaf extract on learning, memory, and hippocampal amyloid precursor protein mRNA expressions in diabetic rats

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Neural Regeneration Research 2008年第1期3卷 29-32页

作者： Xiaofan Zhang Bo Liang Zhifeng Liang Jun Lin grade 2003 of Pharmaceutical School Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China Department of Physiology Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China Department of Pharmacology Pharmaceutical School Guangxi Medical University Nanning 530021 Guangxi Zhuang Autonomous Region China

BACKGROUND： The mechanisms of brain injury Following diabetes could be related to amyloid precursor protein （APP） mRNA overexpression. Studies have shown that Gingko biloba leaf extract （EGb） is effective in promoting functional recovery of the brain after traumatic injury. EGb is also effective in improving central nervous system plasticity and learning and memory functions of the elderly. OBJECTIVE： To study the effects of EGb on learning and memory, as well as hippocampal APP mRNA expression in the brains of diabetic rats, using Morris water maze behavioral testing and reverse transcription polymerase chain reaction （RT-PCR）, respectively. DESIGN： Complete random design, controlled experimental study. SETTING： Department of Pharmacology, Pharmaceutical School, Guangxi Medical University. MATERIALS： A total of 70 male Wistar rats （180-220 g）, 8 weeks old and specific pathogen free, were used for this study. GbE （containing 24.8% flavone glycosides and 6.2% diterpene lactone） was purchased from Guilin Sitejia Natural Plants Pharmaceutical Factory （Guangxi Province, Lot NO. 200405）. Streptozotocin was purchased from Sigma （USA）. Protamine zinc insulin injection was purchased from WANBANG Biochemical Pharmaceutical Co., Ltd. （Xuzhou Jiangsu, China）. METHODS： The experiment was performed in the Experimental Center of Guangxi Medical University from March to October 2005.（1） Experimental intervention： 70 rats were divided randomly into normal control group, diabetic model group （DM group）, diabetic model ＋10 μ g/kg insulin group （DM ＋ Ins group）, diabetic model ＋ 100 mg/kg ginkgo leaf extract group （DM ＋ EGb high-dose group）, and diabetic model ＋ 50 mg/kg ginkgo leaf extract group （DM ＋ EGb low-dose group）; there were 14 rats in each group. Rats with an intraperitoneal （i.p.） injection of citrate buffer solution （pH 4.4） served as the control group. To establish the diabetes model, rats were treated with i.p. injection of 55 mg/kg streptozotocin. Insulin （10 U/kg） was

关键词： Gingko biloba leave extract diabetes hippocampus amyloid precursor protein

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“大学生下基层”的现状与思考

高校辅导员学刊 2010年第1期2卷 68-72页

作者： Party Branch of Graduate Students of grade 2008,Departmentof Chinese Language and Literature,Beijing University(Department of Chinese Language and Literature,Beijing University,Beijing,100871,China) 北京大学中文系2008级硕士党支部

"大学生下基层"是近年国家针对大学毕业生的一项重要政策,本调查小组以大学生村官及支教两个项目为对象,从选择动机、实际情况、自我评价以及未来打算四个方面对下基层大学生进行了实地访谈与调查,在此基础上对如何充分发挥"大学生下基... 详细信息

"大学生下基层"是近年国家针对大学毕业生的一项重要政策,本调查小组以大学生村官及支教两个项目为对象,从选择动机、实际情况、自我评价以及未来打算四个方面对下基层大学生进行了实地访谈与调查,在此基础上对如何充分发挥"大学生下基层"的优势提出了一些建议和思考。

关键词：大学生基层村官支教

白介素18与糖尿病及其并发症相关性的研究进展

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中国全科医学 2011年第9期14卷 935-938页

作者：苏桂兰唐灵桂林医学院2008级研究生班广西桂林市541000 桂林医学院附属医院老年内科

糖尿病及其并发症的发生与慢性低度炎症有关。白介素18(IL-18)作为一种前炎症因子,可能在糖尿病及其并发症的发生、发展中起着重要的作用。IL-18水平变化不但对糖尿病及其并发症的预后和临床治疗的评估有一定的价值,也为防治糖尿病及其... 详细信息

糖尿病及其并发症的发生与慢性低度炎症有关。白介素18(IL-18)作为一种前炎症因子,可能在糖尿病及其并发症的发生、发展中起着重要的作用。IL-18水平变化不但对糖尿病及其并发症的预后和临床治疗的评估有一定的价值,也为防治糖尿病及其并发症提供了新的研究方向。现就IL-18与糖尿病及其并发症的相关性做一综述。

关键词：白介素18 糖尿病糖尿病并发症胰岛素抵抗

奥沙利铂、顺铂分别联合多西他赛用于局部晚期宫颈癌根治术前新辅助化疗的临床研究

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实用妇产科杂志 2011年第12期27卷 908-911页

作者：华诏召刘燕熊员焕南昌大学研究生院医学部2008级江西南昌330006 江西省人民医院江西南昌330006

目的:比较奥沙利铂(OXA)联合多西他赛(DTX)与顺铂(DDP)联合DTX用于ⅠB2～ⅡB期局部晚期宫颈癌患者行根治性手术前新辅助化疗的疗效和毒副反应。方法:选择2009年2月至2010年12月江西省人民医院收治的ⅠB2～ⅡB期局部晚期宫颈癌患者65例,... 详细信息

目的:比较奥沙利铂(OXA)联合多西他赛(DTX)与顺铂(DDP)联合DTX用于ⅠB2～ⅡB期局部晚期宫颈癌患者行根治性手术前新辅助化疗的疗效和毒副反应。方法:选择2009年2月至2010年12月江西省人民医院收治的ⅠB2～ⅡB期局部晚期宫颈癌患者65例,随机分为研究组(35例)和对照组(30例)。研究组采用OT方案(OXA130mg/m2+DTX75mg/m2);对照组采用PT方案(DDP60mg/m2+DTX75mg/m2),每组在根治性子宫切除术前各行新辅助化疗2个周期,并比较两组新辅助化疗的疗效、毒副反应及术后切除子宫标本的病理组织检查情况。结果:①研究组化疗的有效率(97.1%)与对照组(96.7%)比较,差异无统计学意义(P>0.05)。②新辅助化疗毒性反应:研究组WBC减少(Ⅱ度)发生率(5.7%)和N减少(Ⅱ度)发生率(15.7%)均低于对照组(18.3%和31.7%)(P0.05)。结论:OXA+DTX与DDP+DTX两种新辅助化疗方案对局部晚期宫颈癌均有明显效果,OXA+DTX方案总体毒性反应低于DDP+DTX方案,明显改善患者在化疗期间的生活质量。

关键词：奥沙利铂顺铂局部晚期宫颈癌新辅助化疗根治性子宫切除术

冠心病经络腧穴反应的研究及在治未病中的临床运用

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四川中医 2010年第11期28卷 26-28页

作者：杜怀斌梁繁成都中医药大学研究生2008级四川成都610075

目的:通过分析冠心病经络腧穴的病理反应规律,为临床早期防治冠心病提供借鉴。方法:楂阅近10年国内外研究文献,从经络腧穴病理反应发生部位、形式、特性等方面来探讨其临床规律。结果:冠心病经络腧穴的病理反应多表现为病变部位附近、... 详细信息

目的:通过分析冠心病经络腧穴的病理反应规律,为临床早期防治冠心病提供借鉴。方法:楂阅近10年国内外研究文献,从经络腧穴病理反应发生部位、形式、特性等方面来探讨其临床规律。结果:冠心病经络腧穴的病理反应多表现为病变部位附近、胸背部、经脉循行线上、耳部或其他全息单元上,成组出现的穴位色泽、感觉、组织形态和生物物理特性等方面的改变,并具有相对特异性。结论:利用冠心病经络腧穴病理反应的相对特异性和灵敏性,早期干预,及时防治,从而达到"未病先防"的作用。

关键词：冠心病经络腧穴穴位病理反应

通便玉蓉丸对便秘小鼠胃排空和小肠推进的影响

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现代中医药 2011年第3期31卷 52-53页

作者：王欢周永学陕西中医学院 2008级研究生陕西咸阳712046

目的探讨通便玉蓉丸对便秘小鼠排便功能的影响,以阐明其治疗便秘的机理。方法将60只清洁级ICR小鼠随机分为空白组、模型组、麻仁润肠丸组、通便玉蓉丸大、中、小剂量组。经过造模、治疗,检测胃排空率和小肠碳末推进率。结果通便玉蓉丸... 详细信息

目的探讨通便玉蓉丸对便秘小鼠排便功能的影响,以阐明其治疗便秘的机理。方法将60只清洁级ICR小鼠随机分为空白组、模型组、麻仁润肠丸组、通便玉蓉丸大、中、小剂量组。经过造模、治疗,检测胃排空率和小肠碳末推进率。结果通便玉蓉丸促进胃排空作用明显,碳末推进百分率高,碳末移动距离延长,与模型组比较,有显著差异(P<0.05)。结论通便玉蓉丸通过提高胃排空率和小肠碳末推进率,能够增加排便治疗便秘。

关键词：通便玉蓉丸排便小肠推进率

Label-free breast cancer detection and classification by convolutional neural network-based on exosomes surface-enhanced raman scattering

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Journal of Innovative Optical Health Sciences 2023年第2期16卷 3-15页

作者： Xiao Ma Honglian Xiong Jinhao Guo Zhiming Liu Yaru Han Mingdi Liu Yanxian Guo Mingyi Wang Huiqing Zhong Zhouyi Guo MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science SATCM Third grade Laboratory of Chinese Medicine and Photonics Technology&Guangdong Provincial Key Laboratory of Laser Life Science Guangzhou Key Laboratory of Spectral Analysis and Functional Probes College of Biophotonics South China Normal University Guangzhou 510631 P.R.China Department of Physics and Optoelectronic Engineering Foshan University Guangdong 528011P.R.China

Because the breast cancer is an important factor that threatens women's lives and health,early diagnosis is helpful for disease screening and a good *** are nanovesicles,secreted from cells and other body fluids,which can reflect the genetic and phenotypic status of parental *** with other methods for early diagnosis of cancer(such as circulating tumor cells(CTCs)and circulating tumor DNA),exosomes have a richer number and stronger biological stability,and have great potential in early ***,it has been proposed as promising biomarkers for diagnosis of early-stage ***,distinguishing different exosomes remain is a major biomedical *** this paper,we used predictive Convolutional Neural model to detect and analyze exosomes of normal and cancer cells with surface-enhanced Raman scattering(SERS).As a result,it can be seen from the SERS spectra that the exosomes of MCF-7,MDA-MB-231 and MCF-10A cells have similar peaks(939,1145 and 1380 cm^(-1)).Based on this dataset,the predictive model can achieve 95%*** with principal component analysis(PCA),the trained CNN can classify exosomes from different breast cancer cells with a superior *** results indicate that using the sensitivity of Raman detection and exosomes stable presence in the incubation period of cancer cells,SERS detection combined with CNN screening may be used for the early diagnosis of breast cancer in the future.

关键词： Exosomes surface-enhanced Raman scattering(SERS) breast cancer convolutional neural model label-free