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Anti-inflammatory activity of ginsenosides in LPS-stimulated RAW 264.7 cells

Science Bulletin 2015年第8期60卷 773-784,M0003页

作者： Sungeun Ahn Muhammad Hanif Siddiqi Hae-Yong Noh Yu-Jin Kim Yeon-Ju Kim Chi-Gyu Jin Deok-Chun Yang ginseng genetic bank Graduate School of BiotechnologyCollege of Life Science Kyung Hee University

Ginsenosides, the main active constituents of Panax ginseng Meyer （P. ginseng）, have potential therapeutic effects. All tested ginsenosides except gin- senoside F1 have previously been reported in inflammation studies using the RAW 264.7 macrophage cell line. We ex- amined the anti-inflammatory effects of single sugar moiety ginsenosides such as compound K （CK）, Rh2, Rhl, and F1 that were isolated from P. ginseng through in silico docking studies. We investigated their biological activity predictions, including absorption, distribution, metabolism, excretion, and toxicity and PASS properties, on the suppression of NF- κB, followed by in vitro validation in lipopolysaccharide （LPS）-stimulated RAW 264.7 macrophage cells. The molecular docking results of our study showed that all treated ginsenosides are non-toxic and may be drug-like molecules. The molecular binding interactions of these ginsenosides with the active residues of NF-κB noticeably support their anti-inflammatory activity. CK and Rhl sig- nificantly reduced the production of nitric oxide, cyclooxy- genase-2 （COX-2）, and pro-inflammatory cytokines such as prostaglandin E2 and tumor necrosis factor alpha （TNF-α） in a dose-dependent manner. Real-time PCR and Western blot analyses further confirmed that protopanaxadiols （PPDs） and protopanaxatriols （PPTs） inhibitory effects may have been due to the down-regulation of TNF-α, inducible nitric oxide synthase, COX2, nuclear factor kappa B （NF-κB）, and I kappa B kinase. The expression of co-stimulatory molecules such as ROS was also inhibited by CK and Rhl in a dose- dependent manner. Furthermore, activation of NF-κB in LPS-stimulated RAW 264.7 macrophages was significantly suppressed by CK and Rhl. Taken together, these results provide evidence that PPD- and PPT-type ginsenosides in- cluding CK and Rhl may exhibit strong anti-inflammatory effects by inhibiting pro-inflammatory mediators through down-regulation of NF-κB.

关键词： Ginsenosides Protopanaxadiol Protopanaxatriol Inflammation NF-κB/IKK RAW 264.7

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微生物酶催化制备人参皂苷20(S)-Rg2,20(S)-Rh1和20(S)-PPT

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高等学校化学学报 2011年第1期32卷 67-73页

作者：成乐琴金瑜真梁德春吉林化工学院化学与制药工程学院吉林132022 Korean ginseng Center for Most Valuable Products&ginseng genetic Resource bank Kyung Hee UniversitySuwon 449-701Korea

以微生物Microbacterium esteraromaticum GS514的培养液中分离的粗酶为催化剂水解人参皂苷Re和Rg1,并通过1HNMR和13C NMR谱对水解产物的结构进行了表征.实验结果表明,反应体系中无机盐NaCl的存在与否直接影响人参皂苷Re,Rg1与粗酶液的... 详细信息

以微生物Microbacterium esteraromaticum GS514的培养液中分离的粗酶为催化剂水解人参皂苷Re和Rg1,并通过1HNMR和13C NMR谱对水解产物的结构进行了表征.实验结果表明,反应体系中无机盐NaCl的存在与否直接影响人参皂苷Re,Rg1与粗酶液的反应结果.人参皂苷与粗酶液直接反应时,人参皂苷Re不发生反应,人参皂苷Rg1通过C6所连β-D-吡喃葡萄糖的选择性水解转化成人参皂苷F1.而该反应在无机盐NaCl存在下进行时,人参皂苷Re通过对C20所连β-D-吡喃葡萄糖的选择性水解定向转化为20(S)-人参皂苷Rg2;人参皂苷Rg1定向转化成20(S)-人参皂苷Rh1以及20(S)-原人参三醇(PPT).表明NaCl的加入激活了C20β-D-吡喃葡萄糖苷酶的活性,这对定向合成不同次级人参皂苷具有重要意义.

关键词：酶催化制备 20(S)-人参皂苷Rg2 20(S)-人参皂苷Rh1 20(S)-原人参三醇

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Transcriptome-Wide Identification and Functional Analysis of PgSQE08-01 Gene in Ginsenoside Biosynthesis in Panax ginseng ***.

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 313-327页

作者： Lei Zhu Lihe Hou Yu Zhang Yang Jiang Yi Wang Meiping Zhang Mingzhu Zhao Kangyu Wang College of Life Science Jilin Agricultural UniversityChangchun130118China Jilin Engineering Research Center ginseng genetic Resources Development and Utilization Changchun130118China Jilin Academy of Vegetable and Flower Sciences Changchun130033China

Panax ginseng *** an important plant species used in traditional Chinese medicine,whose primary active ingredient is a *** biosynthesis is not only regulated by transcription factors but also controlled by a variety of structural ***,the molecular mechanism underlying ginsenoside biosynthesis has always been a topic in the discussion of ginseng secondary *** epoxidase(SQE)is a key enzyme in the mevalonic acid pathway,which affects the biosynthesis of secondary metabolites such as *** ginseng transcriptome,expression,and ginsenoside content databases,this study employed bioinformatic methods to systematically analyze the genes encoding SQE in *** first selected six PgSQE candidates that were closely involved in ginsenoside biosynthesis and then identified PgSQE08-01 to be highly associated with ginsenoside ***,we constructed the overexpression vector pCAMBIA3301-PgSQE08-01 and the RNAi vector pART27-PgSQE08-01 and transformed ginseng adventitious roots using Agrobacterium rhizogenes,to obtain positive hairy-root ***,quantitative reverse transcriptionpolymerase chain reaction and high-performance liquid chromatography were used to determine the expression of relevant genes and ginsenoside content,***,we focused on the function of PgSQE08-01 gene,which was noted to be involved in ginsenoside ***,these findings not only provided a molecular basis for the identification of important functional genes in ginseng but also enriched genetic resources for the biosynthesis of ginsenosides using synthetic biology.

关键词： Panax ginseng pgSQE08-01 gene squalene epoxidase ginsenoside ginseng hairy roots

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MKK7-mediated phosphorylation of JNKs regulates the proliferation and apoptosis of human spermatogonial stem cells

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World Journal of Stem Cells 2021年第11期13卷 1797-1812页

作者： Zeng-Hui Huang Chuan Huang Xi-Ren Ji Wen-Jun Zhou Xue-Feng Luo Qian Liu Yu-Lin Tang Fei Gong Wen-Bing Zhu Institute of Reproductive and Stem Cell Engineering Central South UniversityChangsha 410008Hunan ProvinceChina Department of Reproductive Center Reproductive and Genetic Hospital of CITIC-XiangyaChangsha 410008Hunan ProvinceChina Department of Sperm bank Reproductive and Genetic Hospital of CITICXiangyaChangsha 410008Hunan ProvinceChina

BACKGROUND Human spermatogonial stem cells(SSCs)are the basis of ***,little is known about the developmental regulatory mechanisms of SSC due to sample origin and species *** To investigates the mechanisms involved in the proliferation of human *** The expression of mitogen-activated protein kinase kinase 7(MKK7)in human testis was identified using immunohistochemistry and western blotting(WB).MKK7 was knocked down using small interfering RNA,and cell proliferation and apoptosis were detected by WB,EdU,cell counting kit-8 and fluorescenceactivated cell *** bioinformatic analysis,the interaction of MKK7 with c-Jun N-terminal kinases(JNKs)was verified by protein co-immunoprecipitation and *** phosphorylation of JNKs was inhibited by SP600125,and the phenotypic changes were detected by WB,cell counting kit-8 and fluorescenceactivated cell *** MKK7 is mainly expressed in human SSCs,and MKK7 knockdown inhibits SSC proliferation and promotes their ***7 mediated the phosphorylation of JNKs,and after inhibiting the phosphorylation of JNKs,the phenotypic changes of the cells were similar to those after MKK7 *** expression of MKK7 was significantly downregulated in patients with abnormal spermatogenesis,suggesting that abnormal MKK7 may be associated with spermatogenesis *** MKK7 regulates the proliferation and apoptosis of human SSC by mediating the phosphorylation of JNKs.

关键词： MKK7 Spermatogonial stem cell Proliferation Apoptosis JNKs

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RNA-seq reveals mechanisms of SlMX1 for enhanced carotenoids and terpenoids accumulation along with stress resistance in tomato

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Science Bulletin 2017年第7期62卷 476-485页

作者： Mohamed Ewas Yangqiang Gao Farhan Ali Elsayed M. Nishawy Raheel Shahzad Hizar Subthain Mohamed Amar Cathie Martin Jie Luo National Key Laboratory of Crop genetic Improvement and National Center of Plant Gene Research (Wuhan) College of Life Sdence and Technology Huazhong Agricultural University Wuhan 430070 China Egyptian Deserts Gene bank (EDGB) Genetic Resources Department Cairo Egypt Deserts Research Center (DRC) Cairo Egypt John lnnes Centre Norwich Research Park Colney Norwich NR4 7UH UK

Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human *** previous study reported that manipulation of Sl MX1 gene enhanced carotenoids accumulation and drought resistance in ***,RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of Sl MX1 involved in stress resistance and enhanced secondary *** analysis showed that overexpression of Sl MX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic(fungus,bacteria,virus and insects) stresses in *** research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome *** addition,we reported two types of trichome which seems to be aberrant types in *** study unravels the mechanism of regulation of Sl MX1,which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.

关键词： Tomato SlMX1 Resistance Carotenoids Terpenoids Mechanism RNA Seq

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Response of the ginseng C_(2)H_(2)-Type Zinc Finger Protein Family PgZFPs Gene to Methyl Jasmonate Regulation

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 3055-3071页

作者： Yue Jiang Lingyu Liu Kangyu Wang Mingzhu Zhao Ping Chen Jun Lei Yanfang Wang Meiping Zhang Yi Wang Guang Chen College of Life Science Jilin Agricultural UniversityChangchun130118China Research Center for ginseng genetic Resources Development and Utilization Jilin ProvinceJilin Agricultural UniversityChangchun130118China College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun130118China

The main active components of ginseng are ginsenosides,which play signiﬁcant roles in treating cardiovascular diseases,cancer,and providing antioxidant *** are primarily synthesized through the mevalo-nate pathway and the methylerythritol phosphate *** key enzyme genes involved in this biosynthetic process have been cloned and validated,yet the regulatory functions of transcription factors remain *** C_(2)H_(2)-type zincﬁnger protein family,one of the largest families of transcription factors,is crucial in plant growth and development,response to biotic and abiotic stresses,and regulation of secondary *** study,based on the ginseng transcriptome database from Jilin,conducted a correlation analysis between the expression levels of PgZFPs genes in the Jilin ginseng C_(2)H_(2)-type zincﬁnger protein family and ginsenoside content,a gen-ome-wide association study of PgZFPs,and co-expression analysis of PgZFPs with validated key enzyme ***,ﬁve candidate genes involved in ginsenoside biosynthesis were identiﬁ*** involvement of PgZFP27 and PgZFP-59-02 genes from the PgZFPs family in the biosynthesis of ginsenosides was validated through in vitro methyl jasmonate(MeJA)induction *** result provides new genetic resources for the biosynthesis of ginsenosides.

关键词： Ginsenosides C_(2)H_(2)-type zincﬁnger protein family MeJA induction functional study SIT SITIENS FLC FLACCA MVA Mevalonate MEP 2-C-methyl-D-erythritol 4-phosphate GWAS Genome-wide association studies HPLC High-performance liquid chromatography

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Shaken not stirred: A molecular contribution to the systematics of genus Mugil (Teleostei, Mugilidae)

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Integrative Zoology 2016年第4期11卷 263-281页

作者： Sandra HERAS Ferruccio MALTAGLIATI Maria Victoria FERNÁNDEZ María Inés ROLDÁN Laboratory of genetic Ichthyology University of GironaGironaSpain Department of Biology University of PisaPisaItaly

With this work we addressed some molecular systematic issues within the Mugil cephalus species ***ticular attention was paid to the debated situations of:(i)Mugil liza,occurring in partial sympatry with Mugil cephalus in the northwestern Atlantic,and(ii)Mugil platanus,considered by some authors a synonymy of the former species and distributed in the southwestern *** sequenced 79 individuals of a 465-bp portion of the mitochondrial control region(CR)from 8 western Atlantic and 2 Mediterranean *** addition,all CR sequences available from Genbank for the studied taxa were added to our dataset,for a total of 323 indi***,229 haplotypes corresponding to 8 divergent monophyletic lineages were *** of phylogenetic analyses were consistent with the occurrence of past speciation events producing the observed lin*** these lineages,7 correspond to cryptic species and one is constituted by *** and *** a matter of fact,these 2 taxa constitute a single lineage within the *** species ***,individuals of ***/*** lineage analyzed by means of the 18 mitochondrial markers available in Genbank exhibited a degree of genetic diversity consistent with highly divergent *** the 8 lineages detected,the Mediterraean one(type locality)corresponds to ***;the lineage ***/*** should be named ***,under the priority principle,and the left 6 lineages need formal description.

关键词： cryptic species historical demography molecular phylogeny Mugil systematics

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Re-evaluating data quality of dog mitochondrial,Y chromosomal,and autosomal SNPs genotyped by SNP array

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Zoological Research 2016年第6期37卷 356-360页

作者： Newton O.OTECKO Min-Sheng PENG He-Chuan YANG Ya-Ping ZHANG Guo-Dong WANG State Key Laboratory of genetic Resources and Evolution Yunnan Key Laboratory of Molecular Biology of Domestic AnimalsGermplasm Bank of Wild SpeciesKunming Institute of ZoologyChinese Academy of Sciences Kunming College of Life Science University of Chinese Academy of Sciences State Key Laboratory for Conservation and Utilization of Bio-Resources Yunnan University

Quality deficiencies in single nucleotide polymorphism （SNP） analyses have important implications. We used missingness rates to investigate the quality of a recently published dataset containing 424 mitochonddal, 211 Y chromosomal, and 160 432 autosomal SNPs generated by a semicustom Illumina SNP array from 5 392 dogs and 14 grey wolves. Overall, the individual missingness rate for mitochondrial SNPs was -43.8%, with 980 （18.1%）individuals completely missing mitochondrial SNP genotyping （missingness rate=l）. In males, the genotype missingness rate was -28.8% for Y chromosomal SNPs, with 374 males recording rates above 0.96. These 374 males also exhibited completely failed mitochondrial SNPs genotyping, indicative of a batch effect. Individual missingness rates for autosomal markers were greater than zero, but less than 0.5. Neither mitochondrial nor Y chromosomal SNPs achieved complete genotyping （locus missingness rate=0）, whereas 5.9% of autosomal SNPs had a locus missingness rate=l. The high missingness rates and possible batch effect show that caution and rigorous measures are vital when genotyping and analyzing SNP array data for domestic animals. Further improvements of these arrays will be helpful to future studies.

关键词： SNP array Dog Mitochondrial Ychromosomal Autosomal

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Origination and Establishment of a Trigenic Reproductive Isolation System in Rice

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Molecular Plant 2016年第11期9卷 1542-1545页

作者： Yidan Ouyang Guangwei Li Jiaming Mi Conghao Xu Hongyi Du Chengjun Zhang Weibo Xie Xianghua Li Jinghua Xiao Huazhi Song Qifa Zhang National Key Laboratory of Crop genetic Improvement and National Centre of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China Germplasm bank of Wild Species Kunming Institute of Botany Chinese Academy of Sciences No. 132 Lanhei Road Kunming Yunnan 650201 China These authors contributed equally to this article

Dear Editor,Reproductive isolation is both the indicator and a primary force of speciation, and plays a key role in maintaining species identity. Understanding the origin and mechanisms of reproductive isola- tion is of fundamental importance in evolutionary biology. In recent years, a number of genes that induce reproductive barriers have been identified in several model organisms such as Drosophila, rodents, yeast, Arabidopsis.

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Characterisation of manganese toxicity tolerance in Arabis paniculata

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Plant Diversity 2021年第2期43卷 163-172页

作者： Ting Tang Faqing Tao Weiqi Li School of Life Sciences Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated UtilizationKey Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted SoilsHunan University of Science and TechnologyXiangtan411201China Plant Germplasm and Genomics Center Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunming650201China

Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food *** paniculata is a pioneer species of Brassicaceae found in mining areas,and has the ability to accumulate heavy ***,little is known about the genetic mechanisms of Mn tolerance in *** this study,we found that Mn tolerance and ability to accumulate Mn were higher in *** than in Arabidopsis *** mechanisms underlying the response and recovery of *** to Mn toxicity were further investigated using transcriptome analysis.A total of 69,862,281 base pair clean reads were assembled into 61,627 high-quality unigenes,of which 41,591(67.5%)and 39,297(63.8%)were aligned in the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO),*** response to Mn toxicity,genes were expressed in twelve distinct patterns,which can be divided into four general categories:initial,stable,dose-dependent,and *** that were differentially expressed during Mn response and recovery belong to several dominant KEGG *** early response to Mn toxicity in *** includes the upregulation of genes involved in glutathione ***-binding cassette(ABC)transporter proteins were up-regulated during the entire response phase,and genes involved in glycerophospholipid metabolism were upregulated during the late phase of the Mn *** in the phenylpropanoid pathway were differentially expressed in the repair process after Mn *** findings reveal ideal material and genetic resources for phytoremediation in Mn-contaminated areas and highlight new knowledge and theoretical perspectives on the mechanisms of Mn tolerance.

关键词： Arabis paniculata Manganese toxicity Transcriptome Gene expression

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