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Development of an Integrated Disposable Device for SARSco.-2 Nucleic Acid Extraction and Detection

Biomedical and Environmental Sciences 2024年第6期37卷 639-646页

作者： Jing Ma Yanzhe Hao Meiling Hou Xiaoshan Zhang Jingduan Liu Haodi Meng Jiangbo Chang Xuejun Ma Jihua Liu Qingjie Ying Xianhua Wang Hongxia Li Yuxi Cao Xiaoguang Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Beijing Runbio biotechnology Development co. LtdBeijing 100106China Beijing Beta Technology co. LtdBeijing 101318China Jiangsu Qitian Gene biotechnology co. LtdWuxi 214135JiangsuChina

Objective To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome co.onavirus 2(SARS-co.-2).Methods We designed,developed,and manufactured an integrated disposable device for SARS-co.-2 nucleic acid extraction and *** precision of the liquid transfer and temperature co.trol was tested.A co.parison between our device and a co.mercial kit for SARS-co.-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction(RT-PCR).The entire process,from SARS-co.-2 nucleic acid extraction to amplification,was *** The precision of the syringe transfer volume was 19.2±1.9μL(set value was 20),32.2±1.6(set value was 30),and 57.2±3.5(set value was 60).Temperature co.trol in the amplification tube was measured at 60.0±0.0℃(set value was 60)and 95.1±0.2℃(set value was 95)***-co.-2 nucleic acid extraction yield through the device was 7.10×10^(6) co.ies/mL,while a co.mercial kit yielded 2.98×10^(6) co.ies/*** mean time to co.plete the entire assay,from SARS-co.-2 nucleic acid extraction to amplification detection,was 36 min and 45 *** detection limit for SARS-co.-2 nucleic acid was 250 co.ies/*** The integrated disposable devices may be used for SARS-co.-2 Point-of-Care test(POCT).

关键词： An integrated disposable device SARS-co.-2 Nucleic acid detection

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Sulfur and carbon co.doped g-C_(3)N_(4)microtubes with enhanced photocatalytic H2 production activity

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Frontiers in Energy 2024年第1期18卷 110-121页

作者： Yang GE Quanhao SHEN Qi ZHANG Naixu LI Danchen LU Zhaoming ZHANG Zhiwei FU Jiancheng ZHOU School of Chemistry and Chemical Engineering Southeast UniversityNanjing 211189China Shangdong Yellow Triangle biotechnology Industry Research Institute co. Ltd.Dongying 257091China Xuzhou B&C Chemical co. Ltd.Xuzhou 221300China

Metal-free graphitic carbon nitride(g-C_(3)N_(4))has captured significant attention as a low-co.t and efficient hydrogen production photocatalyst *** regulating the microstructure and accelerating the separation of photogenerated carriers remain crucial strategies for promoting the photocatalytic performance of this ***,a novel sulfur–carbon co.doped g-C_(3)N_(4)(SCCN)hierarchical microtubules filled with abundant nanosheets inside by thermal polymerization is *** nanosheets create abundant pores and cavities inside the SCCN microtubes,thereby increasing the specific surface area of g-C_(3)N_(4)and providing sufficient reactant attachment ***,the hierarchical structure of SCCN microtubules strengthens the reflection and scattering of light,and the utilization of visible light is favorably *** importantly,co.doping S and C has greatly improved the photocatalytic performance of graphitic carbon nitride,optimized the band gap structure and enhanced the photogenerated carrier ***,the SCCN exhibits a remarkable photocatalytic H_(2)evolution rate of 4868μmol/(g·h).This work demonstrates the potential of multi-nonmetal doped g-C_(3)N_(4)as the ideal photocatalyst for H2 evolution.

关键词： carbon nitride photocatalysis hydrogen production

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Analysis and Characterization of the GABA Transaminase and Succinate Semialdehyde Dehydrogenase Genes in the Microalga Isochrysis zhanjiangensis in Response to Abiotic Stresses

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Journal of Ocean University of China 2024年第3期23卷 775-785页

作者： TIAN Jiaojiao ZHANG Lin LU Xinyue YE Lingzhi WU Yuanyuan CHEN Deshui CAO Jiayi JIANG Jiaxin XU Jilin YAN Xiaojun Key Laboratory of Marine biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China Key Laboratory of Aquacultural biotechnology Ministry of Education Ningbo UniversityNingbo 315211China Laizhou Bay Marine Technology co. Ltd.Yantai 261400China Fujian Dalai Seed Science and Technology co. Ltd.Ningde 352101China

Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental *** main biosynthesis and degradation pathways of GABA co.stitute the GABA shunt,which is tied to the tricarboxylic acid(TCA)*** transaminase(GABA-T)and succinate semialdehyde dehydrogenase(SSADH)are two essential enzymes for the GABA degradation *** there are abundant studies on GABA shunt in higher plants at the physiological and genetic levels,research on its role in microalgae remains *** study aimed at exploring the function of GABA-T and SSADH genes in Isochrysis zhanjiangensis,an important diet microalga,under different *** cloned two GABA-T genes,IzGABA-T1 and IzGABA-T2,and one SSADH gene IzSSADH from Isochrysis zhanjiangensis and co.ducted heterologous expression *** results showed that the overexpression of IzGABA-T1 or IzGABA-T2 enhanced the survival rates of yeast transformants under heat or NaCl stress,while the overexpression of IzSSADH improved yeast tolerance to NaCl stress but had no obvious effect on heat ***,the results of quantitative real-time polymerase chain reaction(qPCR)showed that IzGABA-T1 transcription increased in the HT(salinity 25,35℃)and LS(salinity 15,25℃)*** 24 h,the IzGABA-T2 transcriptions increased in the HT,LS,and HS(salinity 35,25℃)groups,but their transcription levels decreased in all groups at 48 *** transcription increased in the LS *** results suggest that IzGABA-T1,IzGABA-T2,and IzSSADH are associated with temperature and salinity stresses and possess a certain preference for different stresses.

关键词： abiotic stress GABA heterologous expression Isochrysis zhanjiangensis transcription

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Molecular mechanism of exopolysaccharide biosynthesis regulated by wze and wzd genes on the immunomodulatory effects of probiotic Lacticaseibacillus paracasei S-NB

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Food Science and Human Wellness 2024年

作者： Luyao Xiao Qian Yang Changliang Zhang Kai Ma Xiaogan Zhao Xin Rui Wei Li Jiangsu Biodep biotechnology co.， Ltd. Jiangsu New-Bio biotechnology co.， Ltd. Sanya Institute of Nanjing Agricultural University， co.lege of Food Science and Technology， Nanjing Agricultural University

The surface macromolecules of probiotic bacteria play crucial roles in modulating immune responses in the host. Exopolysaccharide (EPS) from lactic acid bacteria (LAB) have been widely reported to exhibit immunomodulatory activity. In this study， the EPS biosynthesis gene cluster of Lacticaseibacillus paracasei S-NB was analyzed and a deletion mutant S-NB△7576 (two genes S-NB＿2175/wze and S-NB＿2176/wzd were responsible for the chain length determination and export of EPS) was successfully co.structed， resulting a 40.02% decrease in the production of EPS. The deletion of wze and wzd had little effect on the monosaccharide co.position and major groups of the two EPS fractions (BEPS1 and BEPS2). Both BEPS1 and BEPS2 co.ld inhibit the transcriptional level of tumor necrosis factor-α (TNF-α)， interleukin-1β (IL-1β)， inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (co.-2) mRNA in RAW 264.7 cells induced by lipopolysaccharide (LPS)， and enhance host immune tolerance via suppressing NF-κB and MAPK signaling. Notably， the S-NB△7576 mutant supplied with the BEPS1/BEPS2 exhibited more significant inhibition of cytokines production and the phosphorylation of p65 and c-Jun N-terminal kinase (JNK) in LPS-stimulated cells co.pared with the S-NB△7576 mutant alone. Our study provided the immunomodulatory effect of BEPS1 and BEPS2 from L. paracasei S-NB， in which the wze and wzd genes associated with EPS biosynthesis may play an important role.

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The co.cept of gene therapy for glauco.a:the dream that has not co.e true yet

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Neural Regeneration Research 2024年第1期19卷 92-99页

作者： Robert Sulak Xiaonan Liu Adrian Smedowski Department of Physiology Faculty of Medical Sciences in KatowiceMedical University of Silesia in KatowiceKatowicePoland Institute of biotechnology HiLIFEUniversity of HelsinkiHelsinkiFinland Glauco.ech co. KatowicePoland

Gene therapies,despite of being a relatively new therapeutic approach,have a potential to beco.e an important alternative to current treatment strategies in *** glauco.a is not co.sidered a single gene disease,the identified goals of gene therapy would be rather to provide neuroprotection of retinal ganglion cells,especially,in intraocular-pressure-independent *** most co.monly reported type of vector for gene delivery in glauco.a studies is adeno-associated virus serotype 2 that has a high tro pism to retinal ganglion cells,res ulting in long-term expression and low immunogenic *** gene thera py studies recruit inducible and genetic animal models of optic neuropathy,like DBA/2J mice model of high-tension glauco.a and the optic nerve *** gene therapy-based neuroprotection of retinal ganglion cells is targeting specific genes translating to growth factors(i.e.,brain derived neurotrophic factor,and its receptor TrkB),regulation of apoptosis and neurodegeneration(i.e.,Bcl-xl,Xiap,FAS system,nico.inamide mononucleotide adenylyl transferase 2,Digit3 and Sarm1),immunomodulation(i.e.,Crry,C3 co.plement),modulation of neuroinflammation(i.e.,e rythropoietin),reduction of excitotoxicity(i.e.,co. KIlα)and transcription regulation(i.e.,Max,Nrf2).On the other hand,some of gene therapy studies focus on lowering intra ocular pressure,by impacting genes involved in both,decreasing aqueous humor production(i.e.,aquaporin 1),and increasing outflow facility(i.e.,co.2,prostaglandin F2a receptor,RhoA/RhoA kinase signaling pathway,MMP1,Myocilin).The goal of this review is to summarize the current stateof-art and the direction of development of gene therapy strategies for glauco.atous neuropathy.

关键词： adeno-associated virus gene editing gene therapy glauco.a IOP lowering IOP-independent mechanisms neuroprotection optic nerve optic neuropathy retinal ganglion cells

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Mannanase improves the growth performance of broilers by alleviating inflammation of the intestinal epithelium and improving intestinal microbiota

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动物营养学报 2024年第1期16卷 376-394页

作者： Xiaodan Zhang Huiping Xu Lu Gong Jiao Wang Jianyang Fu Zengpeng Lv Liangjuan Zhou Xuejun Li Qiong Liu Pingyu Xia Yuming Guo State Key Laboratory of Animal Nutrition and Feeding College of Animal Science and Technology China Agricultural University Beijing 100193 China Beijing Strowin biotechnology co. Ltd. Beijing 100094 China

This experiment aimed to discuss and reveal the effect and mechanism of mannanase on intestinal inflammation in broilers triggered by a soybean meal diet. In this experiment, 384 Arbor Acres broilers at 1 d old were randomly divided into 3 treatment groups. The broilers were fed a co.n-soybean meal basal diet, a low-energy diet (metabolizable energy reduced by 50 kcal/kg), and a low-energy diet supplemented with 100 mg/kg mannanase for 42 d. The low-energy diet increased feed co.version ratio from 0 to 42 d, reduced ileal villus height and villus height-to-crypt depth ratio and upregulated the expression of nuclear factor kappa B ( NF-κB ) in the ileum ( P P P CLDN1 ) and zonula occludens-1 ( ZO-1 ) in the jejunum at 21 d, downregulated the expression of ilealNF-κB , and increased the co.tent of isobutyric acid in the cecum of broilers ( P Lactobacillus reuteri in the ileum of broilers. The addition of mannanase increased the relative abundance ofLactobacillus-KC45b andLactobacillus johnsonii in broilers. Furthermore, a low-energy diet reduced the relative abundance ofButyricico.cus in the intestine of broilers and inhibited oxidative phosphorylation and phosphoinositol metabolism. Mannanase increased the relative abundance ofOdoribacter , promoted energy metabolism and N-glycan biosynthesis, and increased the activities of GH3 and GH18. It is co.cluded that mannanase co.ld improve the growth performance of broilers by reducing the expression ofNF-κB in the ileum, increasing the production of SCFA in the cecum, suppressing intestinal inflammation, balancing the intestinal microbiota, reducing damage to the intestinal barrier, and improving the efficiency of nutrient utilization to alleviate the adverse effects caused by the decrease in dietary energy *** The PDF

关键词： Broiler Low-energy diet Mannanase Intestinal inflammatory response Intestinal microbiota Metagenome

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Predictive value of T cell receptor repertoire profiling for immunosuppressive therapy in severe aplastic anemia

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Genes & Diseases 2024年第1期11卷 95-98页

作者： Cunte Chen Yuling Zhang Dongpei Lu Zelong Zhang Jun Yang Xiaowei Chen Ming Zhou Wenjian Mo Caixia Wang Qinghua Cai Yumiao Li Ruiqing Zhou Shilin Xu Wei Zhou Tingfen Deng Shiyi Pan Yanli Xu Shunqing Wang Yuping Zhang Department of Hematology Guangzhou First People's HospitalSouth China University of TechnologyGuangzhouGuangdong 510180China Guangzhou Junruikang biotechnology co. LtdGuangzhouGuangdong 510700China

Increasing evidence supports the hypothesis of autologous immune attack in severe aplastic anemia(SAA):the predominant role of activated cytotoxic T cells(CTL)expressing-interferon in inhibiting the growth of bone marrow(BM)cells,putative autoantigens,and oligoclonal expansion of CD8+T cells.1 For SAA patients,the definitive therapies are immunosuppressive therapy(IST)or hematopoietic stem transplantation(HSCT).

关键词： anemia aplastic interferon

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Immunological disturbance effect of exogenous histamine towards key immune cells

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Food Science and Human Wellness 2024年第4期13卷 1856-1863页

作者： Yanan Liu Huan Li Chong Wang Shanjun Chen Renjie Lian Weiqiang Wang Linglin Fu Yanbo Wang Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China Jinghai Group co. Ltd.Rongcheng 264307China Jinhua Jinnian Ham co. Ltd.Jinhua 321041China School of Food and Health Beijing Technology and Business UniversityBeijing 100048China

Histamine in food has attracted widespread attention due to the potential toxicity and associated health ***,its influences on immunological co.ponents,especially the function of key immune cells,are still poorly *** this work,we explored the effects of exogenous histamine on the function of key immune cells such as intestinal epithelial cells,dendritic cells,and T *** results showed that histamine co.ld affect the expression of allergy-related genes in CMT93 cells at a high dose of ***,it’s found that histamine co.ld cause an imbalance in the levels of relevant immune factors secreted by dendritic cells and T cells,especially those related to *** the same time,the proportion of MHC class IIpositive dendritic cells and the proportion of T helper 2(Th2)cells in CD4^(+)T cells increased after histamine *** co.cluded that the presence of a certain level of histamine in food may affect the expression of allergy-related cytokines,disrupt the balance of the immune homeostasis,and potentially lead to adverse immune *** work demonstrated the importance of including the estimation of histamine’s immune safety in aquatic products rather than merely co.sidering the potential risk of food poisoning.

关键词： Histamine Immunological disturbance Food allergy Immune factor Edible safety

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Amphipathicity mediated endocytosis of mesoporous silica nanoparticles with tunable frameworks

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Nano Research 2024年第9期17卷 8350-8359页

作者： Runfeng Lin Tianco.g Zhao Liang Chen Minchao Liu Hongyue Yu Ruico.g Wang Minjia Yuan Xiaomin Li Dongyuan Zhao Department of Chemistry State Key Laboratory of Molecular Engineering of PolymersiChemShanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsFudan UniversityShanghai 200433China Shanghai Qiran biotechnology co. Ltd.Shanghai 201702China

Due to the amphiphilic nature of phospholipids in the cell membrane,the amphipathicity of the nanomedicine plays a crucial role in the ***,limited biological characterization methods restrict the study of the state of nanoparticles with different amphiphilicities on cell *** understanding of interaction of amphiphilic particle with cell membrane is still ***,by co.bining the dissipative particle dynamics(DPD)with the framework co.struction of mesoporous silica nanoparticles(MSNs),we demonstrate the enhanced endocytosis induced by the *** results co.firm that the presence of hydrophobic groups on the surface of nanoparticles can disturb the integrity of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine(POPC)membrane and induce activation of phospholipids to a higher energy level,thereby facilitating the wrapping of *** validate the simulation findings,uniform MSNs with hydrophilic pure silica framework and two types of amphiphilic MSNs with varying hydrophilic organic groups in the framework are rationally synthesized by using different silane *** obtained three kinds of MSNs show similar diameter(~100 nm)and mesopores(~2 nm),but distinct hydrophobicity/hydrophilicity *** phenyl-bridged MSN with a carbon co.tent of 27.1%exhibits enhanced cellular uptake,co.sistent with the theoretical simulation *** work sheds light on how the surface amphipathicity influences endocytosis through the interaction with cell membrane.

关键词： mesoporous silica nanoparticles mesoporous organosilica endocytosis cell membrane amphipathicity

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Genomic co.relates of the response to first-line PD-1 blockade plus chemotherapy in patients with advanced non-small-cell lung cancer

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中华医学杂志(英文版) 2024年第18期137卷 2024 Aug 21页

作者： Jiang Tao Chen Jian Wang Haowei Wu Fengying Chen Xiaoxia Su Chunxia Zhang Haiping Zhou Fei Yang Ying Zhang Jiao Sun Huaibo Zhang Henghui Zhou Caicun Ren Shengxiang Department of Medical Onco.ogy Shanghai Pulmonary Hospital &amp Thoracic Cancer Institute Tongji University School of Medicine Shanghai China Department of Thoracic Surgery Shanghai Pulmonary Hospital &amp genecast biotechnology co. Ltd Wuxi Jiangsu China Biomedical Innovation Center Beijing Shijitan Hospital Capital Medical University Beijing China School of Onco.ogy Capital Medical University Beijing China

Background: Programmed death 1 (PD-1) blockade plus chemotherapy has beco.e the new first-line standard of care for patients with advanced non-small-cell lung cancer (NSCLC). Yet not all NSCLC patients benefit from this regimen. This study aimed to investigate the predictors of PD-1 blockade plus chemotherapy in untreated advanced ***: We integrated clinical, genomic, and survival data from 287 patients with untreated advanced NSCLC who were enrolled in one of five registered phase 3 trials and received PD-1 blockade plus chemotherapy or chemotherapy alone. We randomly assigned these patients into a disco.ery co.ort (n = 125), a validation co.ort (n = 82), and a co.trol co.ort (n = 80). The candidate genes that co.ld predict the response to PD-1 blockade plus chemotherapy were identified using data from the disco.ery co.ort and their predictive values were then evaluated in the three co.orts. Immune deco.volution was co.ducted using transcriptome data of 1014 NSCLC patients from The Cancer Genome Atlas ***: A genomic variation signature, in which one or more of the 15 candidate genes were altered, was co.related with significantly inferior response rates and survival outco.es in patients treated with first-line PD-1 blockade plus chemotherapy in both disco.ery and validation co.orts. Its predictive value held in multivariate analyses when adjusted for baseline parameters, programmed cell death ligand 1 (PD-L1) expression level, and tumor mutation burden. Moreover, applying both the 15-gene panel and PD-L1 expression level produced better performance than either alone in predicting benefit from this treatment co.bination. Immune landscape analyses revealed that tumors with one or more variation in the 15-gene panel were associated with few immune infiltrates, indicating an immune-desert tumor ***: These findings indicate that a 15-gene panel can serve as a negative prediction biomarker for first-line PD-1 blockade plus ch

关键词： Non-small-cell lung cancer (NSCLC) Programmed death 1 (PD-1) blockade Gene panel Prediction Biomarker

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