检索结果-南通市图书馆

Journal of Integrative Agriculture 2024年第6期23卷 2144-2148页

作者： Yanjing Liu Qingqing Yu Xiangyu Zhou Wenxin Li Xinwen He Yan Wang Guohua Deng Jianzhong Shi Guobin Tian Xianying Zeng Hualan Chen College of Veterinary Medicine Gansu Agricultural UniversityLanzhou 730070China State key laboratory for animal Disease Control and Prevention Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry DiseasesMinistry of Agriculture and Rural Affairs/Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China National Poultry laboratory animal Resource Center Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China Harbin Weike biotechnology Co. Ltd.Harbin 150009China

H9N2 virus has been widely distributed in wild birds and poultry around the world since its first emergence in the United States of America in 1966(Gu et al.2017;Carnaccini and Perez 2020).The virus appeared in chickens in China in the early 1990s,and over the last two decades has gradually become the dominant epidemic subtype(Sun and Liu 2015;Bi et al.2020).Although H9N2 virus infection alone cannot cause severe disease or death in poultry,H9N2 virus-infected birds experience a degree of egg production drop and can be easily infected by other pathogens,thus causing economic losses for poultry industry.

关键词： H9N2 poultry policy

Mechanisms underlying the role of endoplasmic reticulum stress in the placental injury and fetal growth restriction in an ovine gestation model

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and biotechnology 2024年第1期15卷 201-217页

作者： Hao Zhang Xia Zha Yi Zheng Xiaoyun Liu Mabrouk Elsabagh Hongrong Wang Honghua Jiang Mengzhi Wang laboratory of Metabolic Manipulation of herbivorous animal Nutrition College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Joint International Research laboratory of Agriculture and Agri‑Product Safety Ministry of Education of ChinaYangzhou UniversityYangzhou 225009P.R.China Department of animal Production and Technology Faculty of Agricultural Sciences and TechnologiesNiğde Omer Halisdemir UniversityNigde 51240Turkey Department of Nutrition and Clinical Nutrition Faculty of Veterinary MedicineKafrelsheikh UniversityKafrelSheikhEgypt Department of Pediatrics Northern Jiangsu People’s HospitalClinical Medical CollegeYangzhou UniversityYangzhou 225001China State key laboratory of Sheep Genetic Improvement and Healthy Production Xinjiang Academy of Agricultural Reclamation ScienceShihezi 832000China

Background Exposure to bisphenol A(BPA),an environmental pollutant known for its endocrine-disrupting properties,during gestation has been reported to increase the risk of fetal growth restriction(FGR)in an ovine model of *** hypothesized that the FGR results from the BPA-induced insufficiency and barrier dysfunction of the placenta,oxidative stress,inflammatory responses,autophagy and endoplasmic reticulum stress(ERS).However,precise mechanisms underlying the BPA-induced placental dysfunction,and subsequently,FGR,as well as the potential involvement of placental ERS in these complications,remain to be *** In vivo experiment,16 twin-pregnant(from d 40 to 130 of gestation)Hu ewes were randomly distributed into two groups(8 ewes each).One group served as a control and received corn oil once a day,whereas the other group received BPA(5 mg/kg/d as a subcutaneous injection).In vitro study,ovine trophoblast cells(OTCs)were exposed to 4 treatments,6 replicates *** OTCs were treated with 400μmol/L BPA,400μmol/L BPA+0.5μg/m L tunicamycin(Tm;ERS activator),400μmol/L BPA+1μmol/L 4-phenyl butyric acid(4-PBA;ERS antagonist)and DMEM/F12 complete medium(control),for 24 *** In vivo experiments,pregnant Hu ewes receiving the BPA from 40 to 130 days of pregnancy experienced a decrease in placental efficiency,progesterone(P4)level and fetal weight,and an increase in placental estrogen(E2)level,together with barrier dysfunctions,OS,inflammatory responses,autophagy and ERS in type A *** vitro experiment,the OTCs exposed to BPA for 24 h showed an increase in the E2 level and related protein and gene expressions of autophagy,ERS,pro-apoptosis and inflammatory response,and a decrease in the P4 level and the related protein and gene expressions of antioxidant,anti-apoptosis and barrier ***,treating the OTCs with Tm aggravated BPA-induced dysfunction of barrier and endocrine(the increased E2 level and decreased P4 level),OS,inflamm

关键词： Autophagy Bisphenol A Endoplasmic reticulum stress Fetal growth restriction Inflammatory responses Sheep

Pseudorabies virus VHS protein abrogates interferon responses by blocking NF-κB and IRF3 nuclear translocation

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2024年第4期39卷 587-599页

作者： Zhenfang Yan Jiayu Yue Yaxin Zhang Zhengyang Hou Dianyu Li Yanmei Yang Xiangrong Li Adi Idris Huixia Li Shasha Li Jingying Xie Ruofei Feng key laboratory of biotechnology and Bioengineering of State Ethnic Biomedical Research Center Northwest Minzu UniversityLanzhou730030China College of Life Science and Engineering Northwest Minzu UniversityLanzhou730030China gansu Tech Innovation Center of animal Cell Biomedical Research CenterNorthwest Minzu UniversityLanzhou730030China Engineering Research Center of key Technology and Industrialization of Cell-based Vaccine Ministry of EducationBiomedical Research CenterNorthwest Minzu UniversityLanzhou730030China Centre for Immunology and Infection Control School of Biomedical SciencesQueensland University of TechnologyKelvin GroveQueensland4702Australia

Herpesviruses antagonize host antiviral responses through a myriad of molecular strategies culminating in the death of the host *** virus(PRV)is a significant veterinary pathogen in pigs,causing neurological sequalae that ultimately lead to the animal's *** is known to trigger apoptotic cell death during the late stages of *** virion host shutdown protein(VHS)encoded by UL41 plays a crucial role in the PRV infection *** this study,we demonstrate that UL41 inhibits PRV-induced activation of inflammatory cytokine and negatively regulates the cGAS-STING-mediated antiviral activity by targeting IRF3,thereby inhibiting the translocation and phosphorylation of ***,mutating the conserved amino acid sites(E192,D194,and D195)in the RNase domain of UL41 or knocking down UL41 inhibits the immune evasion of PRV,suggesting that UL41 may play a crucial role in PRV's evasion of the host immune response during *** results enhance our understanding of how PRV structural proteins assist the virus in evading the host immune response.

关键词： Pseudorabies virus(PRV) UL41 IRF3 Interferon cGAS-STING

Variation of DRB1 Gene in Tibetan Sheep

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Life Sciences 2015年第6期9卷 255-261页

作者： Li Shaobin Luo Yuzhu Hu Jiang Wang Jiqing Liu Xiu Yan Wei gansu key laboratory of herbivorous animal biotechnology Faculty of Animal Science and Technology Gansu AgricultureUniversity Lanzhou 730070 China

It reveals that the MHC （major histocompatibility complex） gene product always involved in the control of immune response and disease resistance. Nowadays many studies have indicated the OLA （ovine lymphocyte antigen） DRB1 gene is associated with some sheep diseases. Tibetan sheep is one of the three major shag sheep breeds in China, and also have the largest number of China＇s sheep breeds. But till now no report has been seen on studying DRB1 gene in Tibetan sheep of China. To understand the evolution and provide the basis for sheep disease resistance, polymorphism in the exon2 ofDRB1 gene in Tibetan sheep was analyzed. The PCR-SSCP, cloning and sequencing were used to analyse DRB1 gene variation in 600 Tibetan sheep of China. And the genetic relationship and evolutionary significance of the alleles had also been analyzed. Total of 31 alleles were identified, in which 15 alleles had not been reported before. And there were 70 SNPs （single nucleotide polymorphisms） sites in 31 sheep DRB1 gene haplotypes, the proportion was 29.5% to the whole exort2 sequence. All of this indicated that DRB1 exon2 is highly polymorphic in Tibetan sheep. The variation identified here might have an impact on both the function and level of expression of the OLA-DRB1.

关键词： Tibetan sheep PCR-SSCP DRB1 gene polymorphism genetic variation.

Variation in the goat KRTAP6-1 gene and its effect on wool traits in Longdong Cashmere goat

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Variation in the goat KRTAP6-1 gene and its effect on wool t...

Identification of Circular RNA in sheep mammary gland using RNA-Seq technology

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Tao Li Shaobin Li Yuzhu Luo Jiqing Wang Xiu Liu Jiang Hu Faculty of animal Science and Technology Gansu Key Laboratory of Herbivorous Animal BiotechnologyGansu Agricultural University

Keratin-associated proteins（KAPs） are a key structural component of wool and hair fibres. They form a semi-rigid matrix in which the keratin intermediate filaments are embedded1 and hence they play an important role in determining fibre physico-mechanical properties. KAP6 belong to high glycine-tyrosine keratin-associated protein（HGT-KAP）. Some research found that the variations in KRTAP6 may have an effect on hair and wool traits in human, sheep and rabbit, but its effect on cashmere traits in goats is still unknown. PCR-SSCP and DNA sequencing technology were used to detect the variation of KRTAP6-1 in 304 Longdong Cashmere goat. GLMs were used to compare various cashmere traits among goats that had different KRTAP6-1 genotypes. Five PCR-SSCP banding patterns（A-E） and twelve single nucleotide polymorphisms（SNPs） of different sequences were identified in Longdong Cashmere goat. The presence of A was associated with an increase of cashmere fiber weight, and the genotype AB of goat had an effect on cashmere fiber weight and curly fiber length. This suggests that KRTAP6-1 may have important value for genetic markers of cashmere in goats.

关键词： KRTAP6-1 variation wool PCR-SSCP

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification of Circular RNA in sheep mammary gland using ...

Variation in the caprine keratin-associated protein 15-1（KAP15-1） gene affects cashmere fibre diameter

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Zhiyun Hao Jiqing Wang Yuzhu Luo Shaobin Li Mengli Zhao Xiu Liu Jiang Hu gansu key laboratory of herbivorous animal biotechnology Faculty of Animal Science and TechnologyGansu Agricultural University

Lactation is primarily responsible for the survival rate of multiple-born lambs. Exploring the molecular mechanism underlying the mammary gland and lactation plays an important role on regulating milk yield and quality. Circular RNA（circRNA） is a particular type of non-coding RNA and has a large number of microRNA（miRNA） binding sites and act as miRNA sponges in cells. These circRNAs can remove the inhibition of miRNAs on their target genes and increasing expression of target genes. In several species, there was a little bit study on circRNA of mammary gland. However, no studies on circRNA of mammary gland in sheep have been reported so far. In our study, expression profile of circRNAs were investigated in mammary gland of two sheep breeds with different milk production characteristics, gansu Alpine Merino（GAM, n=9） and Small Tailed Han sheep（STH, n=9） in peaking-lactating period using RNASeq. The 4,907 circRNAs were found and 33 were differentially expressed circRNAs between the two breed sheep. The 18 circRNAs were up-regulated and 15 circRNAs were down-regulated in STH. The potential target sites of circRNAs were predicted and the RNA-Seq results were validated by qRT-PCR. GO and KEGG results showed differentially expressed circRNAs were mainly enriched in cell proliferation, cell differentiation, signal transduction and other biological processes. This study provides an overview of the circRNA expression profile in the ovine mammary gland and the interaction between some key circRNAs and their target genes, aiming at improving our knowledge of the critical roles of circRNA in sheep milk synthesis.

关键词： CircRNA mammary gland sheep RNA-Seq differentially expressed circRNA

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Variation in the caprine keratin-associated protein 15-1（KA...

Highly efficient generation of GGTA1 knockout pigs using a combination of TALEN m RNA and magnetic beads with somatic cell nuclear transfer

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Mengli Zhao Huitong Zhou Jon G.H.Hickford Jiqing Wang Jiang Hu Xiu Liu Shaobin Li Yuzhu Luo Faculty of animal Science and Technology Gansu Key Laboratory of Herbivorous Animal BiotechnologyGansu Agricultural University

Keratin-associated proteins（KAPs） are a structural component of cashmere fibre, and variation in some KAP genes（KRTAPs） has been associated with a number of caprine fibre traits. In this study, we report the identification of KRTAP15-1 in goats. Sequence variation in the gene was detected using the polymerase chain reaction single-strand conformation polymorphism（PCR-SSCP） technique in 250 Longdong goats, and six variants（named A to F） containing eight single nucleotide polymorphisms（SNPs）, were identified. Five of the SNPs were non-synonymous, and would lead to putative amino acid changes. Reverse transcription-polymerase chain reaction（RT-PCR） analysis revealed that KRTAP15-1 was expressed in secondary hair follicles, but not in heart tissue, liver tissue, lung tissue, kidney tissue, or the longissimus dorsi muscle. Despite being rich in cysteine, the caprine KAP15-1 protein possesses a high content of serine, and moderate content of glycine and phenylalanine. Association analyses revealed that KRTAP15-1 variant A was associated with decreased mean fibre diameter（MFD）, and this effect appeared to be dominant;while variant C was found to be associated with increased MFD, the effect being recessive. The findings suggest that caprine KRTAP15-1 is highly polymorphic and that variation in this gene affects cashmere MFD.

关键词： Keratin-associated protein KAP15-1 KRTAP15-1 cashmere mean fibre diameter tissue expression goat

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2016年第7期15卷 1540-1549页

作者： FENG Chong LI Xi-rui CUI Hui-ting LONG Chuan LIU Xia TIAN Xing-hua PAN Deng-ke LUO Yu-zhu gansu key laboratory of Herbivores animal biotechnology College of Animal Science and TechnologyGansu Agricultural UniversityL anzhou 730070P.R.China key laboratory Farm animal Genetic Resources and Germplasm Innovation Ministry of Agriculture/Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China State key laboratory of Cellular Stress Biology School of Life SciencesXiamen UniversityXiamen 361102P.R.China laboratory of Biochemistry and Molecular Biology College of Life ScienceHenan UniversityKaifeng 475000P.R.China

The transcription activator-like effector nuclease （TALEN） technique combined with the somatic cel nuclear transfer （SCNT） method has been successfuly applied for creating geneticaly modiifed pigs. However, methods for isolating cels with bialelic indels requires further improvement because of the relatively low enrichment efifciency of mutated somatic cels. Moreover, little is known regarding the off-target effects of the TALEN system and the heredity of TALEN-modiifed pigs. In this study, an efifcient method to increase the enrichment efifciency of TALEN-mediated bialelic knockout （KO） cels was established, and corresponding geneticaly modiifed pigs with the expected genotype were generated whose off-target effect, fertility and heredity characteristics were aslo evaluated. Two TALEN pairs were constructed to target the porcine α-1,3-galactosyltransferase （GGTA1） gene locus. TALEN mRNA was transfected into the ear ifbroblasts folowed by the enrichment of α-Gal nul cels of minipigs using isolectin B4 （IB4） lectin and magnetic beads. A total of 115 cel colonies were formed and validated to beGGTA1 KO cels by sequencing and 10 bialelic KO cel colonies were used as nuclear donors for SCNT. ThirtyGGTA1 bialelic KO piglets were successfuly delivered and grew normaly. Seventeen potential off-target sites were investigated, and no off-target events were detected in the live piglets. To determine the fertility and heredity characteristics of TALEN-modiifed pigs, 10 mature founders were mated with each other and the mutations were determined to be transmitted to the F1 piglets. We established a robust and safe technology for developing geneticaly modiifed pig lines with expected genotypes for agricultural breeding and biomedical application.

关键词： transcription activator-like effector nuclease (TALEN) magnetic beads somatic cel nuclear transfer (SCNT) off-target geneticaly modiifed pigs

Rescued virus from infectious cDNA clone of rabbit hemorrhagic disease virus is adapted to RK13 cells line

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2006年第14期51卷 1698-1702页

作者： LIU Guangqing NI Zheng YUN Tao ZHANG Yuying DU Qingyun SHENG Zutian LIANG Huali HUA Jionggang LI Shuangmao Chen Jianping key laboratory of Virology & biotechnology of Agriculture Ministry Zhejiang Academy of Agriculture Sciences Hangzhou 310021 China animal Veterinary Medicine College Gansu Agricultural UniversitylLanzhou 730070 China

Based on the infectious full-length cDNA clone of rabbit hemorrhagic disease virus (RHDV), the in vitro transcripts are introduced into RK13 cells. 12 h later, CPE could be observed clearly, and virual antigen could also be detected by IFA. The titre of the recovered virus is 104.6/mL. Immune electron micro-scopic observation of the virus particles revealed that the particles were rotund with a diameter of about 30 nm. Besides, virus titre quantification obtained by qRT-PCR showed a correlation between time from infection and virus titre. All these results showed that we have recovered RHDV from RK13 cells by re-verse genetics technology successfully, and this would be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and new type vaccines of RHDV.

关键词：兔出血性疾病 RHDV 反求遗传学 RK13细胞 cDNA

Whole-genome resequencing reveals molecular imprints of anthropogenic and natural selection in wild and domesticated sheep

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2022年第5期43卷 695-705页

作者： De-Yin Zhang Xiao-Xue Zhang Fa-Di Li Lv-Feng Yuan Xiao-Long Li Yu-Kun Zhang Yuan Zhao Li-Ming Zhao Jiang-Hui Wang Dan Xu Jiang-Bo Cheng Xiao-Bin Yang Wen-Xin Li Chang-Chun Lin Bu-Bo Zhou Wei-Min Wang State key laboratory of Grassland Agro-Ecosystems Key Laboratory of Grassland Livestock Industry InnovationMinistry of Agriculture and Rural AffairsEngineering Research Center of Grassland IndustryMinistry of EducationCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhouGansu 730020China College of animal Science and Technology Gansu Agricultural UniversityLanzhouGansu 730070China Engineering laboratory of Sheep Breeding and Reproduction biotechnology in gansu Province MinqinGansu 733300China Lanzhou Veterinary Research Institute Chinese Academy of Agricultural SciencesLanzhouGansu 730046China

The abundance of domesticated sheep varieties and phenotypes is largely the result of long-term natural and artificial selection. However, there is limited information regarding the genetic mechanisms underlying phenotypic variation induced by the domestication and improvement of sheep. In this study, to explore genomic diversity and selective regions at the genome level, we sequenced the genomes of 100 sheep across 10 breeds and combined these results with publicly available genomic data from 225 individuals, including improved breeds, Chinese indigenous breeds,African indigenous breeds, and their Asian mouflon ancestor. Based on population structure, the domesticated sheep formed a monophyletic group,while the Chinese indigenous sheep showed a clear geographical distribution trend. Comparative genomic analysis of domestication identified several selective signatures, including IFI44 and IFI44L genes and PANK2 and RNF24 genes, associated with immune response and visual *** genomic analysis of improvement demonstrated that candidate genes of selected regions were mainly associated with pigmentation,energy metabolism, and growth ***, the IFI44 and IFI44L genes showed a common selection signature in the genomes of 30domesticated sheep breeds. The IFI44 c. 54413058C>G mutation was selected for genotyping and population genetic validation. Results showed that the IFI44 polymorphism was significantly associated with partial immune traits. Our findings identified the population genetic basis of domesticated sheep at the whole-genome level, providing theoretical insights into the molecular mechanism underlying breed characteristics and phenotypic changes during sheep domestication and improvement.

关键词： Sheep Whole-genome resequencing Selection signature analysis Immunity IFI44 gene