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Knock-out of BnHva22c reduces the susceptibility of Brassica napus to infection with the fungal pathogen Verticillium longisporum

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The Crop Journal 2024年第2期12卷 503-514页

作者： Wanzhi Ye Roxana Hossain Michael Prbsting Abdallah Abdelmegid Mohamed Ali Lingyue Han Ying Miao Steffen Rietz Daguang Cai Dirk Schenke Department of Molecular Phytopathology and biotechnology Institute of PhytopathologyChristian-Albrechts-University of KielKielGermany Institut für Zuckerrübenforschung GöttingenGermany Plant pathology Department Faculty of Agriculture and Natural ResourcesAswan UniversityAswanEgypt Fujian Provincial Key laboratory of Plant Functional Biology College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Hohenlieth-Hof NPZ Innovation GmbHHoltseeGermany

Verticillium longisporum(Vl43)is a soilborne hemibiotrophic fungal pathogen causing stem striping on oilseed rape(OSR)and severe yield *** for resistant varieties is the most promising approach to control this ***,we report the identification of Hva22c as a novel susceptibility factor and its potential for improving OSR ***22c is a member of the Hva22 gene family,originally described for barley(Hordeum vulgare).Several Hva22 members have been located at the endoplasmic ***22c is up-regulated in response to Vl43 in both Arabidopsis and *** demonstrate that knock-out of Hva22c in OSR by CRISPR/Cas9 and its homolog in Arabidopsis by T-DNA insertion reduced plants’susceptibility to Vl43 infection and impaired the development of disease *** understand the underlying mechanism,we analysed transcriptomic data from infected and non-infected roots of hva22c knock-out and wild type *** identified a homozygous mutant with frame-shifts in all four BnHva22c loci displaying a vastly altered transcriptional landscape at 6 ***,a large set of genes was suppressed under mock conditions including genes related to the endomembrane *** the up-regulated genes we found several defense-related and phytohormone-responsive genes when comparing mutant to the wild *** results demonstrate that Hva22c is functionally required for a fully compatible plant-fungus *** loss of function reduces plant susceptibility,most likely due to endoplasmatic reticulum and Golgi dysfunction accompanied by additionally activated defense *** findings can help improve OSR resistance to *** infection.

关键词： Hva22c Susceptibility factor CRISPR/Cas9 Brassica napus Arabidopsis Verticillium longisporum Resistance breeding

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A Kunitz trypsin inhibitor from chickpea (<i>Cicer arietinum</i>L.) that exerts an antimicrobial effect on Fusarium oxysporum ***. ciceris

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Agricultural Sciences 2013年第11期4卷 585-594页

作者： Meera Nair Sardul Singh Sandhu fungal biotechnology and invertebrate pathology laboratory Department of Biological Sciences Rani Durgavati University Ja-balpur India

Fusarium oxysporum ***. ciceris (Foc) is one of the most important fungal pathogens of chickpea and is regarded as a constant threat in tropical and subtropical countries. In order to correlate Fusarium wilt resistance/susceptibility in Cicer arietinum to the presence or absence of trypsin inhibitor (TI) in the crude extract, trypsin inhibitory assay (TIA) and in vitro activity of TI against Foc were studied. In the present study, a 20 kDa trypsin inhibitor was purified from Fusarium wilt resistant cultivar (viz. JG 2001-12) by ammonium sulfate precipitation, dialysis and chromatographies with Sephadex G-100 and Diethyl aminoethyl cellulose (DEAE-cellulose-52) ion-exchange column. Results of pathogenecity assay were found to be in correlation to the trypsin inhibitor assay where the Fusarium wilt resistant cultivar showed high trypsin inhibitory activity (99%) in the presence of trypsin enzyme using both natural and synthetic substrates. Preliminary studies using crude extracts of JG 2001-12 showed a decrease in radial growth of Foc. A 45%-82% reduction in conidium germination at 20 μg·mL-1?Cicer arietinum trypsin inhibitor (CaTI) concentration was observed, thereby, indicating the use of CaTI in suppression of pathogen and in its deployment through transgenic plants for the management of Fusarium wilt.

关键词： Cicer arietinum Fusarium Wilt Kunitz Proteinase Inhibitor Trypsin Inhibitor

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Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

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Journal of Integrative Plant Biology 2015年第7期57卷 641-652页

作者： Fen Lu Huiqin Wang Shanzhi Wang Wendi Jiang Changlin Shan Bin Li Jun Yang Shiyong Zhang Wenxian Sun Department of Plant pathology China Agricultural University Key laboratory in Plant pathology Ministry of AgricultureChina Agricultural University State Key laboratory of Rice Biology and Key laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of AgricultureInstitute of BiotechnologyZhejiang University Rice Research Institute Shandong Academy of Agricultural Science

The elongation factor Tu （EF-Tu） receptor （EFR） in cruciferous plants specifically recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants （Oryza sativa L. ssp. japonica cv. Zhonghua 17） and cell cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We＇demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa- tion in the AtEFR transgenic rice cells and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatically in transgenic rice plant and cell lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig- gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expressionlevel. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Collectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

关键词： Bacterial disease resistance elongation factor Tu receptor interspecific gene transfer pattern recognition receptor rice

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Mate-recognition and species boundaries in the ascomycetes

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fungal Diversity 2013年第1期58卷 1-12页

作者： Simon H.Martin Emma T.Steenkamp Michael J.Wingfield Brenda D.Wingfield Department of Genetics Forestry and Agricultural Biotechnology InstituteUniversity of PretoriaPretoria 0002South Africa Department of Microbiology and Plant pathology Forestry and Agricultural Biotechnology InstituteUniversity of PretoriaPretoria 0002South Africa

Reproductive gene evolution is commonly invoked as a source of reproductive isolation during *** possibility has not been adequately explored in the Ascomycota,the most species-rich fungal *** mechanisms of mate-recognition in this group are relatively simple:a“mating type”locus determines reproductive mode and sexual compatibility,and two pheromone/receptor pairs control sexual ***,ascomycete reproductive genes can experience unique and interesting evolutionary forces,which could lead to rapid *** this review,we examine the mechanisms of sexual interaction in ascomycetes and explore current evidence as to whether these mechanisms allow for species-specificity in *** discuss the evolutionary forces that can drive reproductive gene divergence,how these may apply in the world of ascomycetes,and their possible consequences for speciation.

关键词： Ascomycota Speciation Mating type Pheromone Receptor

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Fusarium-produced vitamin B6 promotes the evasion of soybean resistance by Phytophthora sojae

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植物学报:英文版 2023年第9期65卷 2204-2217页

作者： Shuchen Wang Xiaoyi Zhang Zhichao Zhang Yun Chen Qing Tian Dandan Zeng Miao Xu Yan Wang Suomeng Dong Zhonghua Ma Yuanchao Wang Xiaobo Zheng Wenwu Ye Department of Plant pathology Nanjing Agricultural UniversityNanjing 210095China Key laboratory of Plant Immunity Nanjing Agricultural UniversityNanjing 210095China Key laboratory of Soybean Disease and Pest Control(Ministry of Agriculture and Rural Affairs) Nanjing Agricultural UniversityNanjing 210095China Key laboratory of Molecular Biology of Crop Pathogens and Insects Institute of BiotechnologyZhejiang UniversityHangzhou 310058China

Plants can be infected by multiple pathogens concurrently in natural systems. However,pathogen–pathogen interactions have rarely been studied. In addition to the oomycete Phytophthora sojae, fungi such as Fusarium spp. also cause soybean root rot. In a 3-year field investigation, we discovered that P. sojae and Fusarium spp. frequently coexisted in diseased soybean roots. Out of 336 P. sojae–soybean–Fusarium combinations,more than 80% aggravated disease. Different Fusarium species all enhanced P. sojae infection when co-inoculated on soybean. Treatment with Fusarium secreted non-proteinaceous metabolites had an effect equal to the direct pathogen coinoculation. By screening a Fusarium graminearum mutant library, we identified Fusarium promoting factor of Phytophthora sojae infection 1(Fpp1),encoding a zinc alcohol dehydrogenase. Fpp1 is functionally conserved in Fusarium and contributes to metabolite-mediated infection promotion, in which vitamin B6(VB6) produced by Fusarium is key. Transcriptional and functional analyses revealed that Fpp1 regulates two VB6 metabolism genes, and VB6 suppresses expression of soybean disease resistance-related genes. These results reveal that co-infection with Fusarium promotes loss of P. sojae resistance in soybean, information that will inform the sustainable use of diseaseresistant crop varieties and provide new strategies to control soybean root rot.

关键词： fungal metabolites pathogen–host interaction Phytophthora sojae soybean root rot vitamin B6

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Multiple functions of SWI/SNF chromatin remodeling complex in plant-pathogen interactions

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Stress Biology 2021年第1期1卷 215-229页

作者： Yunqing Jian Won-Bo Shim Zhonghua Ma State Key laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and InsectsInstitute of BiotechnologyZhejiang UniversityHangzhouChina Department of Plant pathology and Microbiology Texas A&M UniversityCollege StationTXUSA

The SWI/SNF chromatin remodeling complex utilizes the energy of ATP hydrolysis to facilitate chromatin access and plays essential roles in DNA-based *** in animals,plants and fungi have uncovered sophisticated regulatory mechanisms of this complex that govern development and various stress *** this review,we summarize the composition of SWI/SNF complex in eukaryotes and discuss multiple functions of the SWI/SNF complex in regulating gene transcription,mRNA splicing,and DNA damage *** review further highlights the importance of SWI/SNF complex in regulating plant immunity responses and fungal ***,the potentials in exploiting chromatin remodeling for management of crop disease are presented.

关键词： Chromatin remodeling SWI/SNF complex Transcription Splicing DNA damage repair Plant-pathogen interaction

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Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2007年第2期8卷 88-97页

作者： JIN Qing-chao DONG Hai-tao PENG You-liang CHEN Bao-shan SHAO Jing DENG Ye DAI Cheng-en FANG Yong-qi LOU Yi-chun LI You-zhi LI De-bao Bioinformatics and Gene Network Research Group School of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Key laboratory of Molecular Plant pathology Ministry of Agriculture China Agricultural University Beijing 100094 China laboratory of Subtropical Bioresource Conservation and Utilization Guangxi University Nanning 530004 China

Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles ofappressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST （expressed sequence tag） database ofM. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate carboxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTHll, beta subunit of G protein and SGTI involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results.

关键词： Magnaporthe grisea Mature appressoria cDNA array Gene expression profile

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Pathogenomics and Bioinformatic Platforms for fungal Plant Pathogens

Pathogenomics and Bioinformatic Platforms for Fungal Plant P...

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中国植物病理学会第九届全国会员代表大会暨2010年学术年会

作者： Yong-Hwan Lee Department of Agricultural biotechnology Center for Fungal Pathogenesisand Center for Fungal Genetic ResourcesSeoul National UniversitySeoul 151-921Korea

The research goal of our research group is to elucidate the molecular mechanisms of fungal pathogenesis and interactions between rice blast pathogen,Magnaporthe oryzae and its host plant,rice at the genomic *** blast is a compelling model system for studying host-parasite interactions due to its socioeconomic impact and the availability of both the

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ZmGns,a maize class I β-1,3-glucanase,is induced by biotic stresses and possesses strong antimicrobial activity

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Journal of Integrative Plant Biology 2015年第3期57卷 271-283页

作者： Yu-Rong Xie Yenjit Raruang Zhi-Yuan Chen Robert L.Brown Thomas E.Cleveland Department of Plant pathology and Crop Physiology Louisiana State University Agricultural Center biotechnology Research Institute the Chinese Academy of Agricultural Sciences Southern Regional Research Center USDA-ARS

Plant b-1,3-glucanases are members of the pathogenesis-related protein 2（PR-2） family,which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress *** of the differentially expressed proteins（spot 842） identified in a recent proteomic comparison between five pairs of closely related maize（Zea mays L.） lines differing in aflatoxin resistance was further investigated in the present ***,the corresponding cDNA was cloned from maize and designated as *** encodes a protein of338 amino acids containing a potential signal *** expression of Zm Gns was detectible in all tissues studied with the highest level in *** was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus *** was also induced by most abiotic stresses tested and growth hormones including salicylic *** vivo,ZmGns showed a significant inhibitory activity against thebacterial pathogen Pseudomonas syringae *** DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in *** high level of expression in the silk tissue and its induced expression by phytohormone treatment,as well as by bacterial and fungal infections,suggest it plays a complex role in maize growth,development,and defense.

关键词： β-1 3-Glucanase biological activity expression profiles Zea mays ZmGns

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Development of Molecular Marker Linked with Cercospora Leaf Spot (CLS) Disease Resistance in Vigna radiata, Cloning, and Expression for Evaluating Antifungal Activity against Cercospora canescens

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1289-1300页

作者： Maria Babar Siddra Ijaz Imran Ul Haq Muhammad Sarwar Khan Centre of Agricultural Biochemistry and biotechnology(CABB) University of Agriculture FaisalabadFaisalabad38040Pakistan Department of Plant pathology University of Agriculture FaisalabadFaisalabad38040Pakistan

We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence(MB-CLsRG)of identified RGAs(MB-ClsRCaG1 and MB-ClsRCaG2).The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot(CLS)resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic *** of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic *** sequenced PCR products showed 100%homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in *** antifungal potential of MB-CLsRG gene encoding protein was *** MB-CLsRG gene sequence was cloned in the *** expression vector for recombinant protein *** recombinant protein was then investigated for its in vitro antifungal potential against Cercospora *** in vitro investigation showed strong antifungal activity of recombinant protein as it restricted the growth of fungal mycelial *** results validated the linkage of developed marker with CLS-resistant mungbean varieties;therefore,it can be used to screen resistant varieties from a large population in ***,the recombinant protein of the MB-CLsRG gene sequence revealed antifungal potential,which proved the gene sequence could be suitable to use in transgenic plants technology to develop fungal-resistant transgenic crops.

关键词： Molecular marker mungbean MAS recombinant protein antifungal potential

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