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Journal of Integrative Plant Biology 2020年第6期62卷 761-776页

作者： Shuang Miao Jiuer Liu Jianhang Guo Jian-Feng Li Guangdong Provincial Key laboratory of Plant Resources State Key Laboratory of BiocontrolMOE Key Laboratory of Gene Function and RegulationSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Plant cells mount plenty of pattern-recognition receptors(PRRs)to detect the microbe-associated molecular patterns(MAMPs)from potential microbial *** are overrepresented by proteinaneous patterns,such as the flg22 peptide from bacterial *** of PRR receptor complex components by forward or reverse genetics can be time/labor-consuming,and be confounded by functional ***,we present a strategy for identifying PRR complex components by engineering plants to inducibly secrete affinity-tagged proteinaneous MAMPs to the *** PRR protein complexes bound to self-secreted MAMPs are enriched through affinity purification and dissected by mass *** a proof of principle,we could capture the flg22 receptor FLS2 and co-receptor BAK1 using Arabidopsis plants secreting FLAG-tagged flg22 under ***,we identified receptor-like kinases LIK1 and PEPR1/PEPR2 as potential components in the FLS2 receptor complex,which were further validated by protein–protein interaction assays and the reverse genetics *** study showcases a simple way to biochemically identify endogenous PRR complex components without overexpressing the PRR or using chemical crosslinkers,and suggests a possible crosstalk between different immune receptors in plants.A modest dose of estradiol can also be applied to inducing enhanced immunity in engineered plants to both bacterial and fungal pathogens.

关键词： immunity complex mount

Stagonosporopsis pogostemonis:A Novel Ascomycete Fungus Causing Leaf Spot and Stem Blight on Pogostemon cablin（Lamiaceae）in South China

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Stagonosporopsis pogostemonis:A Novel Ascomycete Fungus Caus...

Changes in soil faunal density and microbial community under altered litter input in forests and grasslands

中国植物病理学会2021年学术年会

作者：罗梅黄影华 MANAWASINGHE Ishara sandeepani Dhanushka WANASINGHE LIU Jiawei SHU Yongxin ZHAO Minping XIANG Meimei 董章勇 Innovative Institute for Plant Health Zhongkai University of Agriculture and Engineering CAS Key laboratory for Plant biodiversity and Biogeography of East Asia Kunming Institute of Botany

Pogostemon cablin,is one of the well-known southern Chinese medicinal plants with detoxification,anti 一 bacterial,anti-inflammatory and other pharmacological *** and characterization of phytopathogens on *** are of great significance for the prevention and control of the *** the spring to summer of 2019 and 2020,a leaf spot disease on Pogostemon cablin was observed in Guangdong Province,South *** disease incidence was 15% under high temperature and *** cablin plants with necrotic leaf spots were collected from the field in Zhanjiang City,Guangdong Province,China（E110°3′,N21°2′） in the spring and summer of *** pathogen was isolated and identified based on both morphological and DNA-based molecular *** molecular identification was conducted using multi-gene sequences analysis of large subunit（LSU）,the nuclear ribosomal internal transcribed spacer（ITS）,beta-tubulin（β-tubulin） and RNA polymerase Ⅱ（rpb2） *** isolates were obtained in this *** combined sequence data set comprised of these two Stagonosporopsis isolates from this study and 76 reference *** causal organism was identified as Stagonosporopsis pogostemonis,a novel fungal *** of Stagonosporopsis pogostemonis on *** was fulfilled via confining the Koch’s postulates,causing leaf spots and stem blight *** symptoms were characterized by brown spots from the leaf *** some cases,these spots can coalesce and form a big scorch-like spot covering a large portion of the ***,some of them form perforation,wither and dropped *** pogostemonis showed the similar symptoms to those in field conditions.

关键词： didymellaceae phoma-like pathogenicity phylogeny

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Fundamental Research 2022年第6期2卷 954-963页

作者： Xinyu Wei Fuzhong Wu Petr Heděnec Kai Yue Yan Peng Jing Yang Xiaoyue Zhang Xiangyin Ni Key laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education School of Geographical SciencesFujian Normal UniversityFuzhou 350007China Agritec Plant Research Ltd. Zemědělská16Šumperk 78701Czech Republic Institute of Tropical biodiversity and Sustainable Development University Malaysia TerengganuKuala NerusTerengganu 21030Malaysia

Root and foliar litter inputs are the primary sources of carbon and nutrients for soil fauna and microorganisms,yet we still lack a quantitative assessment to evaluate the effects of root and foliar litter on various groups of soil organisms across terrestrial ***,we compiled 978 paired observations from 68 experimental sites to assess the directions and magnitudes of adding and removing foliar and root litter on the soil faunal density and microbial biomass that was evaluated by phospholipid fatty acids(PLFAs)across forests and grasslands *** found that litter addition had only a marginal effect on soil faunal density but significantly increased the soil total microbial-,fungal-and bacterial-PLFAs by 13%,14%,and 10%,respectively,across ecosystems,suggesting that the soil microbial community is more sensitive to carbon source addition than soil fauna,particularly in soils with low carbon to nitrogen *** contrast,removing litter significantly decreased the soil faunal density by 17%but had few effects on soil *** with foliar litter,root litter input had a more positive effect on the development of soil fungal *** effect of both litter addition and removal on soil faunal density and microbial biomass did not differ between humid and arid regions,but a greater influence was observed in grasslands than in forests for soil microbial *** results highlight that the increasing litter production under a global greening scenario would stimulate microbial activity in grasslands more than in forests,and this stimulation would be greater for soil microbes than soil fauna.

关键词： Detritus input and removal treatment Soil fauna Bacteria Fungi Phospholipid fatty acids

Split Nano luciferase complementation for probing protein-protein interactions in plant cells

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Journal of Integrative Plant Biology 2020年第8期62卷 1065-1079页

作者： Feng-Zhu Wang Nannan Zhang Yan-Jun Guo Ben-Qiang Gong Jian-Feng Li Guangdong Provincial Key laboratory of Plant Resources State Key Laboratory of BiocontrolMOE Key Laboratory of Gene Function and RegulationSchool of Life SciencesSun Yat‐sen UniversityGuangzhou 510275China Guangdong Provincial Key laboratory of Sugarcane Improvement and Biorefinery Guangdong Bioengineering InstituteGuangzhou 510316China

Deciphering protein-protein interactions(PPIs)is fundamental for understanding signal transduction pathways in *** split firefly luciferase(Fluc)complementation(SLC)assay has been widely used for analyzing ***,concern has risen about the bulky halves of Fluc interfering with the functions of their fusion *** luciferase(Nluc)is the smallest substitute for Fluc with improved stability and ***,we developed a dual-use system enabling the detection of PPIs through the Nluc-based SLC and co-immunoprecipitation *** was realized by coexpression of two proteins under investigation in fusion with the HA-or FLAG-tagged Nluc halves,*** validated the robustness of this system by reproducing multiple previously documented PPIs in protoplasts or Agrobacterium-transformed *** next applied this system to evaluate the homodimerization of Arabidopsis CERK1,a coreceptor of fungal elicitor chitin,and its heterodimerization with other homologs in the absence or presence of ***,split fragments of Nluc were fused to two cytosolic ends of Arabidopsis calcium channels CNGC2 and CNGC4 to help sense the allosteric change induced by the bacterial elicitor ***,these results demonstrate the usefulness of the Nluc-based SLC assay for probing constitutive or inducible PPIs and protein allostery in plant cells.

关键词： system interactions Arabidopsis

Biomineralized nanoparticles for the immobilization and degradation of crude oil-contaminated soil

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Nano Research 2023年第10期16卷 12238-12245页

作者： Daoqing Liu Qianwei Li Enhui Liu Miao Zhang Jicheng Liu Chunmao Chen Key laboratory of Water and Sediment Sciences(Ministry of Education) College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China State Key laboratory of Heavy Oil Processing State Key Laboratory of Petroleum Pollution ControlChina University of Petroleum(Beijing)Beijing 102249China

Accidental oil leaks and spills often cause server soil pollution,and in situ remediation is a powerful and economical treatment *** during in situ remediation process,unpredicted migration of petroleum hydrocarbon in heterogeneous soil will lead to a long-term source of persistent aquifer *** reduce the migration of petroleum hydrocarbon and effectively improve the in situ remediation efficiency,herein,fungal biomineralization strategy was proposed for the immobilization of petroleum contaminants.A ureolytic fungi strain with crude oil-degradation ability was screened and identified as Chaetomium *** incubated in medium containing Ca2+and crude oil,a mineral corona with spiny nanoparticles was formed at the edge of oil and the interface characters were analyzed using fluorescent pH and dissolved oxygen(DO)sensing films,*** indicated that biominerals preferred to aggregate around the edge of crude oil,providing favorable microenvironment for fungal growth and then leading to the increase of pH in the microenvironment,eventually accompanied by the formation of mineral *** mineral corona with numerous nanoparticles may act as a solid and stable shell,limiting or reducing the mobility of crude oil,and providing enough time for fungal *** 28 days incubation,oilcontaminated soil treated with fungal biomineralization showed better immobilization ability for total petroleum hydrocarbon(TPH)under simulated acid-rain condition and higher TPH removal *** is the first demonstration for the immobilization of oil through fungal biomineralized nanoparticles,thus providing a novel strategy for the in situ remediation of oilcontaminated sites.

关键词： biomineralization degradation nanomineral nanoaggregates immigration

Exploration the regulation Genes of Microsclerotia Development in Nomuraea rileyi

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Exploration the regulation Genes of Microsclerotia Developme...

A six-gene phylogenetic overview of Basidiomycota and allied phyla with estimated divergence times of higher taxa and a phyloproteomics perspective

中国菌物学会2016年学术年会

作者：宋章永 Youping Yin 王中康 Chongqing Engineering Research Center for fungal Insecticide School of Life ScienceChongqing University

Over the past 50 years,the control of insects with fungal pathogens has been a very active of research and has resulted in a large number of commercially-available *** than 150 entomopathogens products have been commercialized until *** 75% of these products:Metarhizium anisopliae,Beauveria bassiana,*** and Isaria *** routine component is conidia,naturally-infective propagule and production via liquid and solid substrate ***,other fungi,such as Nomuraea rileyi and ***,are not suited for solid substrate conidia *** are urgent requirements to find alternative active propagule of these fungi for commercial ***（MS） with a diameter of 50-600 μm,are pseudoparenchymatous aggregations of hyphae,comprising only a few *** are produced as overwintering propagules by many phytopathogenic *** can survive long periods in adverse environments by producing *** biological applications,MS had been successfully induced in submerged liquid culture fermentation in many entomogenous fungi,such as Metarhizium spp.,***,***,and *** experiments using MS granules to biocontrol assay demonstrated that the MS granules can be used as a mycoinsecticide for management of insect *** Microbial laboratory of Chongqing University had successfully induced MS production of *** in liquid amended medium（AM） and had investigated the molecular mechanism of MS formation through comparative transcriptome analysis and found that internal oxidative stress could trigger MS *** results of investigation showed that exogenous low dose of H2O2 or menadione could contribute to MS ***,exogenous ascorbic acid inhibits MS *** research works on the functional genes of the respiratory chain of *** verificated that the reactive oxygen species（ROS） were generated and related to MS *** the mecha

关键词： Entomogenous Fungi Microsclerotia Molecular Mechanism

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fungal Diversity 2017年第3期84卷 43-74页

作者： Rui-Lin Zhao Guo-Jie Li Santiago Sanchez-Ramirez Matt Stata Zhu-Liang Yang Gang Wu Yu-Cheng Dai Shuang-Hui He Bao-Kai Cui Jun-Liang Zhou Fang Wu Mao-Qiang He Jean-Marc Moncalvo Kevin D.Hyde State Key laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesChaoyang DistrictBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesHuairou DistrictBeijing 100408China Department of Ecology and Evolutionary Biology University of TorontoTorontoON M5S 3B2Canada Department of Natural History Royal Ontario MuseumTorontoON M5S 2C6Canada Key laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Institute of Microbiology and Beijing Key laboratory for Forest Pest Control Beijing Forestry UniversityBeijing 100083China Center of Excellence in fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Key laboratory of Economic Plants and Biotechnology Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina

In this paper,we provide a phylogenetic overview of Basidiomycota and related phyla in relation to ten years of DNA based phylogenetic studies since the AFTOL publications in *** selected 529 species to address phylogenetic relationships of higher-level taxa using a maximumlikelihood framework and sequence data from six genes traditionally used in fungal molecular systematics(nrLSU,nrSSU,5.8S,tef1-a,rpb1 and rpb2).These species represent 18 classes,62 orders,183 families,and 392 genera from the phyla Basidiomycota(including the newly recognized subphylum Wallemiomycotina)and Entorrhizomycota,and 13 species representing 13 classes of Ascomycota as outgroup *** also conducted a molecular dating analysis based on these six genes for 116 species representing 17 classes and 54 orders of Basidiomycota and *** we performed a phyloproteomics analysis from 109 Basidiomycota species and 6 outgroup taxa using amino-acid sequences retrieved from 396 orthologous *** of higher taxa follows the criteria in Zhao et al(fungal Divers 78:239–292,2016):(i)taxa must be monophyletic and statistically well-supported in molecular dating analyses,(ii)their respective stem ages should be roughly equivalent,and(iii)stem ages of higher taxa must be older than those of lower level *** time-tree indicates that the mean of stem ages of Basidiomycota and Entorrhizomycota are ca.530 Ma;subphyla of Basidiomycota are 406–490 Ma;most classes are 358–393 Ma for those of Agaricomycotina and 245–356 Ma for those of Pucciniomycotina and Ustilaginomycotina;most orders of those subphyla split 120–290 *** of most higherlevel taxa of Basidiomycota are generally supported,especially those taxa introduced in the recent ten years:phylum Entorrhizomycota,classes Malasseziomycetes,Moniliellomycetes,Spiculogloeomycetes,Tritirachiomycetes and orders Amylocorticiales,Golubeviales,Holtermanniales,Jaapiales,Lepidostromatales,Robbauerales,Stereopsidales and Tricho

关键词： Fungi Systematics Taxonomy Wallemiomycotina

Asperochones A and B,two antimicrobial aromatic polyketides from the endophytic fungus Aspergillus ***-2

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Chinese Chemical Letters 2024年第9期35卷 206-209页

作者： Hong Zhang Cui-Ping Li Li-Li Wang Zhuo-Da Zhou Wen-Sen Li Ling-Yi Kong Ming-Hua Yang Jiangsu Key laboratory of Bioactive Natural Product Research and State Key laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China

Two novel fungal metabolites,asperochones A and B,were obtained from an Aspergillus *** structures were determined by 1D/2D nuclear magnetic resonance(NMR)spectroscopy,high resolution electrospray ionization mass spectroscopy(HRESIMS),and single-crystal X-ray diffraction *** A possesses an intriguing skeleton bearing 5/6/6/6/7/5/5/5 octacyclic ring system,and asperochone B also exhibits an unusual carbon skeleton with five stereochiral *** structures were proposed as heterotrimeric and heterodimeric products of aromatic *** addition,asperochone A exhibited a potential anti-tuberculosis effect since it showed a moderate potency against Mycobacterium smegmatis.

关键词： Aspergillus sp. Asperochones A and B Aromatic polyketides Heterotrimeric compound Anti-tuberculosis effect Antifibrotic activity

A novel pH adaptation regulator in Candida albicans

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A novel pH adaptation regulator in Candida albicans

Chemodiversity of soil dissolved organic matter affected by contrasting microplastics from different types of polymers

多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： Tong Jiang Changbin Chen Unit of Pathogenic fungal Infection ＆ Host Immunity Institute Pasteur of Shanghai Chinese Academy of Sciences

As an important human conditional pathogenic fungus, Candida albicans’ s ability to sense and adapt to environmental changes is crucial for their survival and reproduction in the host. However, the pH value of different organs varies greatly, and the pH value of the host environment is often in a dynamic process. Therefore,the ability to sense and adapt to the extracellular pH value is the key to the success of symbiosis and pathogenesis of Candida albicans in the host. As an opportunistic pathogen, Candida albicans can perceive different pH of the host environment, especially the alkaline pH, which is an important mechanism for symbiosis to pathogenic transformation. Through preliminary laboratory work, we found a new transcription factor Ppc1 that plays an important regulatory role in the adaptation to alkaline pH. Currently, researches on alkaline pH sensing of Candida albicans mainly focus on conservative Rim101/PacC pathway. By comparing with RIM101 knockout strain, we found that the PPC1 knockout strain is more sensitive to the alkaline pH value. RNA-seq suggested that RIM101 and PPC1 are both involved in the Candida albicans neutral alkaline pH adaptation process and play a key role,but the alkaline-dependent genes regulated by PPC1 and RIM101 are not identical. Quantitatively, PPC1 regulated more alkaline-dependent genes. GO analysis revealed PPC1 and RIM101 regulate similar biological pathways which indicated that Ppc1 is a novel neutral-alkaline pH adaptation regulator in Candida albicans. An in-depth exploration of the interaction mechanism between Ppc1 and Rim101 in the process of regulating the environmental pH adaptation of Candida albicans is of great significance to deepen the understanding of the symbiosis-pathogenic transformation mechanism of Candida albicans and to discover new methods of curing fungal infection.

关键词： Candida albicans pH adaptation RNA-seq

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Frontiers of Environmental Science & Engineering 2023年第12期17卷 149-162页

作者： Hong Yu Beidou Xi Lingling Shi Wenbing Tan State Key laboratory of Environmental Criteria and Risk Assessment and State Environmental Protection Key Laboratory of Simulation and Control of Groundwater PollutionChinese Research Academy of Environmental SciencesBeijing 100012China

Chemodiversity of dissolved organic matter(DOM)is a crucial factor controlling soil nutrient availability,greenhouse gas emissions,and pollutant ***(MPs)are widespread pollutants in terrestrial ecosystems in many ***,the effects of MPs on DOM chemodiversity are not sufficiently understood,particularly under different types of *** UV-Vis spectroscopy,3D fluorescence spectroscopy,and Fourier-transform ion cyclotron resonance mass spectrometry,the effects of three prevalent MPs[polyethylene,polystyrene,and polyvinyl chloride(PVC)]on the chemical properties and composition of soil DOM were investigated via a 310-d soil incubation *** results showed that MPs reduced the aromatic and hydrophobic soil DOM components by more than 20%,with PVC MPs having the greatest ***,as MP contents increase,the humification level of soil DOM significantly *** increased DOM molecules with no heteroatom by 8.3%-14.0%,but decreased DOM molecules with nitrogen content by 17.0%-47.8%.This may be because MPs cause positive“priming effect,”resulting in the breakdown of bioavailable components in soil *** is also related to MPs changing microbial richness and diversity and enriching microbial communities involved in lignin compositions *** the presence of MPs,soil DOM chemodiversity depended on soil pH,electrical conductivity,dissolved organic carbon,soil organic matter,bacterial Shannon,and fungal Chao ***,DOM in MP-contaminated soils featured more lipids and less condensed aromatics and proteins/amino sugars,thereby conferring a lower DOM aromaticity and higher lability.

关键词： Microplastics Dissolved organic matter FT-ICR MS Chemodiversity Molecular signature