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Cell Iines from diamondback moth exhibiting differential susceptibility to baculovirus infection and expressing midgut genes

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Insect Science 2019年第2期26卷 251-262页

作者： Xiao-Li Ma Wei-Yi He Ping Wang Min-Sheng You State Key Laboratory of ecological Pest control for fujian and taiwan crops Fujian Agriculture and Forestry University Fuzhou China fujian -taiwan joint centre for ecological control of crop pests Fujian Agriculture and Forestry University Fuzhou China Key Laboratory of Integrated Pest Management for fujian-taiwan crops Ministry of Agriculture Fujian Agriculture and Forestry University Fuzhou China Institute of Applied Ecology Fujian Agriculture and Forestry University Fuzhou China Department of Entomology Cornell University Geneva NY USA

Abstract Six new cell lines were established from embryonic tissues of the diamondback moth, Plutella xylostella (L.). The cell lines showed differential characteristics, including growth in attachment or in suspension, susceptibility to a baculovirus infection and expression of genes involved in the glucosinolate detoxification pathway in R xylostella larvae. Five of the cell lines grew attached to the culture flask and one cell line grew unattached as a suspension cell line. The cell lines had population doubling times ranging from IS to 23 h. Among five of the P. xylostella cell lines examined for infection of a nucleopolyhe. drovirus from Autographa californica, AcMNPV four cell lines were highly susceptible to AcMNPV infection, but one was only semi-permissive to AcMNPV infection. The production of two recombinant proteins, a β-galactosidase of bacterial origin and a secreted alkaline phosphatase of eukaryotic origin, in the R xylostella cell lines was examined in comparison with that in the cell line Sf9 which is commonly used for recombinant protein production. In the P. xylostella cell lines, expression of three important midgut genes involved in the glucosinolate detoxification pathway, including the glucosinolate sulfatase genes GSS1 and GSS2 and the sulfatase modifying factor gene SUMF1、was detected. The R xylostella cell lines developed in this study could be useful in in vitro research systems for studying insec-virus interactions and complex molecular mechanisms in glucosinolate detoxification and insect-plant interactions.

关键词： AcMNPV cell lines glucosinolate sulfatase (GSS) Plutella xylostella PxGV sulfatase modifying factor 1 (SUMF1)

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Mechanism of plant immune activation and signaling: Insight from the first solved plant resistosome structure

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Journal of Integrative Plant Biology 2019年第8期61卷 902-907页

作者： Baomin Feng Dingzhong Tang State Key Laboratory of ecological control of fujian-taiwan crop pests Key Laboratory of Ministry of Education for Genetics Breeding and Multiple Utilization of Crops Plant Immunity Center Fujian Agriculture and Forestry University

Life or death?It’s a question animal and plant cells ask themselves during development and throughout their lives–and when they choose the latter option,cells can execute a genetically programmed set of cellular functions that result in cell *** animals and plants,the execution of programmed cell death is critical for proper development and for immunity against invading microbes.

关键词： genetically invading microbes

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Population characteristics of Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae) reared on a mushroom fly Coboldia fuscipes (Diptera: Scatopsidae)

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Insect Science 2019年第2期26卷 322-332页

作者： Mei-Fang Wen Hsin Chi Ying-Xiao Lian Yu-Hao Zheng Qing-Hai Fan Min-Sheng You State Key Laboratory of ecological Pest control for fujian and taiwan crops Fujian Agriculture and Forestry University Fuzhou China Institute of Applied Ecology Fujian Agriculture and Forestry University Fuzhou China fujian-taiwan joint centre for ecological control of crop pests Fujian Agriculture and Forestry University Fuzhou China Key Laboratory of Integrated Pest Management for fujian-taiwan crops Ministry of Agriculture Fuzhou China Department of Plant Production and Technologies Faculty of Agricultural Sciences and Technologies Omer Halisdemir University Nigde Turkey Plant Health & Environment Laboratory Ministry for Primary Industries Auckland New Zealand

Subterranean predatory mites are important biological control agents of pests in soil. In order to understand the population characteristics of two predatory mites, Macrocheles glaber Miiller and Stratiolaelaps scimitus Womersley, we studied their development, survival and fecundity data under laboratory conditions using Coboldia fuscipes Meigen as a food source and analyzed them with the age-stage, two-sex life table. Macrocheles glaber had a significantly shorter developmental time, oviposition period, longevity and lower fecundity than those of S. scimitus. The intrinsic rate of increase (λ), finite rate of increase (r), net reproductive rate (C0),net predation rate (C0), and finite predation rate (ω) of M. glaber were significantly lower than those of S. scimitus. Both population parameters and computer simulation implied that S. scimitus is a potential powerful biocontrol agent compared to M. glaber.

关键词： biocontrol life table predatory mites

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Structure and expression of sulfatase and sulfatase modifying factor genes in the diamondback moth,Plutella xylostella

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Insect Science 2018年第6期25卷 946-958页

作者： Xiao-Li Ma Wei-Yi He Wei Chen Xue-Jiao Xu Wei-Ping Qi Ming-Min Zou Yan-Chun You Simon W.Baxter Ping Wang Min-Sheng You State Key Laboratory of ecological Pest control for fujian and taiwan crops Fujian Agriculture and Forestry UniversityFuzhouChina. Institute of Applied Ecology Fujian Agriculture and Forestry UniversityFuzhouChina. fujian-taiwan joint centre for ecological control of crop pests Fujian Agriculture and Forestry UniversityFuzhouChina. Key Laboratory of Integrated Pest Management for fujian-taiwan crops Ministry of AgricultureFuzhouChina. Department of Entomology Comell UniversityNew York State Agricultural Experiment StationGenevaNYUSA . School of Biological Sciences University of AdelaideSouth AustraliaAustralia.

The diamondback moth,Plutella xylostella (L.),uses sulfatases (SULF)to counteract the glucosinolate-myrosinase defensive system that cruciferous plants have evolved to deter insect *** activity is regulated by post-translational modi- fication of a cysteine residue by sulfatase modifying factor 1(SUMF1).We identified 12 SULF genes (PxylSulfs)and two SUMF1 genes (PxylSumfls)in the *** genome. Phylogenetic analysis of SULFs and SUMFs from ***,Bombyx mori,Manduca sexta,Heliconius melpomene,Danaus plexippus,Drosophila melanogaster,Tetranychus urticae and Homo sapiens showed that the SULFs were clustered into five groups,and the SUMFs could be divided into two *** of the expression of PxylSulfs and Pxyl Sumfs by RNA-seq and by quantitative real-time polymerase chain reaction showed that two glucosinolate sulfatase genes (GSS),PxylSulf2and PxylSulf3,were primarily expressed in the midgut of 3rd-and 4th-instar ***,expression of sulfatases PxylSulf2, PxylSulf3 and PxylSulf4 were correlated with expression of the sulfatases modifying fac tor *** findings from this study provide new insights into the structure and expression of SUMF1and PxylSulf genes that are considered to be key factors for the evolutionary success *** as a specialist herbivore of cruciferous plants.

关键词： evolutionary arms race glucosinolate detoxification glucosinolate sulfatase phylogenetie analysis transcriptome analysis

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The OsBSK1-2-MAPK module regulates blast resistance in rice

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The crop Journal 2024年第1期12卷 110-120页

作者： Shengping Li Xinquan Xiang Zhijuan Diao Na Xia Ling Lu Jing Zhang Zhiwei Chen Dingzhong Tang State Key Laboratory of ecological control of fujian-taiwan crop pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina College of Life Science Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Ministerial and Provincial joint Innovation centre for Safety Production of Cross-Strait crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina fujian Provincial Key Laboratory of crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina

Receptor-like cytoplasmic kinase OsBSK1-2 was reported to play an important role in regulation of response to rice blast,but the signaling pathway remained *** this study,we identified OsMAPKKK18 and previously uncharacterized MAPKKKs OsMAPKKK16 and OsMAPKKK19 that interact with *** of all three MAPKKKs was induced by Magnaporthe oryzae infection,and all three induced cell death when transiently expressed in Nicotiana benthamiana *** of OsMAPKKK16 and OsMAPKKK18 compromised blast resistance and overexpression of OsMAPKKK19 increased blast resistance,indicating that all three MAPKKKs are involved in regulation of rice blast ***,both OsMAPKKK16 and OsMAPKKK19 interacted with and phosphorylated OsMKK4 and OsMKK5,and chitin-induced MAPK activation was suppressed in osmapkkk16 and osbsk1-2 ***18 was earlier reported to interact with and phosphorylate OsMKK4 and affect chitin-induced MAPK activation,suggesting that OsBSK1-2 is involved in regulation of immunity through multiple MAPK signaling *** BSK1 in Arabidopsis,OsBSK1-2 was not involved in response to avirulent *** *** together,our results revealed important roles of OsMAPKKK16/18/19 and a OsBSK1-2-OsMAPKKK16/18/19-OsMKK4/5 module in regulating response to rice blast.

关键词： Disease resistance Magnaporthe oryzae Oriza sativa Rice blast

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Rice sl-MH-1 mutant induces cell death and confers blast resistance via the synergistic roles of signaling systems

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The crop Journal 2022年第6期10卷 1755-1766页

作者： Dagang Tian Yan Lin Shengping Li Yiyang Cao Gang Li Xinrui Guo Ziqiang Chen Zaijie Chen Feng Wang Zonghua Wang fujian Key Laboratory of Genetic Engineering for Agriculture Biotechnology Research InstituteFujian Academy of Agricultural SciencesFuzhou 350003FujianChina State Key Laboratory of ecological control of fujian-taiwan crop pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou 350003FujianChina

Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic *** rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have identified an sl mutation(a null mutation of SL)in several rice varieties and confirmed its increase of resistance and cell ***,a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is *** performed a multi-omics analysis to clarify the fundamental mechanisms at the protein,gene transcript,and metabolite *** found that cell death and Magnaporthe oryzae(***)infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid(SA),jasmonic acid(JA),and abscisic acid(ABA)in multiple regulatory *** characterized the dynamic changes of several key hormone levels during disease progression and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease ***-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to *** studies shed light on cell death and facilitate further mechanistic dissection of programmed cell death in rice.

关键词： Sekiguchi lesion Cell death Phytohormone M.oryzae Rice

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CRISPR/Cas9-based functional analysis of yellow gene in the diamondback moth,Plutella xylostella

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Insect Science 2021年第5期28卷 1504-1508,I0002页

作者： Yajun Wang Yuping Huang Xuejiao Xu Zhaoxia Liu Jianyu Li Xue Zhan Guang Yang Minsheng You Shijun You State Key Laboratory of ecological Pest control for fujian-taiwan crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China joint International Research Laboratory of ecological Pest control Ministry of EducationFuzhou 350002China Key Laboratory of Integrated Pest Management for fujian-taiwan crops Ministry of AgricultureFuzhou 350002China ADepartment of Physiology&Neurobiology University of ConnecticutStorrsCT 06269USA fujian Key Laboratory for Monitoring and Integrated Management of crop pests Institute of Plant ProtectionFujian Academy of Agricultural SciencesFuzhou 350013China

The diamondback moth,Plutella xylostella(L.),is an economically important pest of cruciferous crops *** pest is notorious for rapid evolution of the resistance to diferent classes of insecticides,making it increasingly dificult to ***-based control approaches,through manipulation of target genes,have been reported as promising supplements or alternatives to traditional methods of pest *** we identified a gene of pigmentation(yellow)in ***,Pxyellow,which encodes 1674 bp complementary DNA sequence with four exons and three *** the clustered regularly interspersed palindromic repeats(CRISPR)CRISPR-associated protein 9 system,we knocked out Pxyellow,targeting two sites in Exon III,to generate 272 chimeric mutants(57%of the CRISPR-treated individuals)with color-changed phenotypes of the Ist to 3rd instar larvae,pupae,and adults,indicating that Pxyellow plays an essential role in the body pigmentation of P *** analysis revealed no significant difference in the oviposition of adults,the hatchability of eggs,and the weight of pupac between homozygous mutants and wildtypes,suggesting that Pxyellow is not directly involved in regulation of growth,development,or *** work advances our understanding of the genetic and insect science molecular basis for body pigmentation of P xylostella,and opens a wide avenue for development of the genctcally based pest control techniques using Pxyellow as a screening marker.

关键词： CRISPR/Cas9 diamondback moth genetically based control novel marker yellow gene

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CRISPR/Cas9-mediated knockout of period reveals its function in the circadian rhythms of the diamondback moth Plutella xylostella

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Insect Science 2023年第3期30卷 637-649页

作者： Danfeng Wang Jing Chen Yao Yuan Lingqi Yu Guang Yang Wenfeng Chen State Key Laboratory of ecological Pest control for fujian and taiwan crops Institute of Applied EcologyFujan Agriculture and Forestry UniversityFuzhouChina joint International Research Laboratory of ecological Pest control Ministry of EducationFuzhouChina Key Laboratory of Integrated Pest Management for fujian-taiwan crops Ministry of AgricultureFuzhouChina Key Laboratory of Green control of Insect pests(fujian Agriculture and Forestry University) Fujian Province UniversityFuzhouChina Ministerial and Provincial joint Innovation centre for Safety Production of Cross-Strait crops Fujian Agriculture and Forestry UniversityFuzhouChina Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouChina

Circadian clocks control the rhythmicity of many behaviors and physiological features of *** study the circadian clock of the moth Plutella xylostella,we employed CRISPR/Cas9-mediated genome editing to investigate the effect of loss of the clock gene period on the circadian *** harbors a single copy of *** analysis showed that *** PERIOD is more homologous to mouse PERIOD than the PERIOD proteins from bees,flies,mosquitos,and many other Lepidoptera,such as Danaus plexippus and Bombyx *** circadian rhythms in adult locomotor activity were altered in the period knockout strain of *** under light–dark(LD)and continuous dark(DD)*** the LD cycle,the wild-type moths displayed nocturnal activity with activity peaking very early after lights off and quickly declining after lights *** contrast,the period knockout strain had no peak in activity when the lights were turned off and exhibited steady activity throughout the hours of ***,under DD conditions,our results showed that the locomotor rhythm can be maintained without period gene,but at a lower rhythmicity ratio than *** addition,knockout of period in *** changed circadian rhythms patterns related to pupal eclosion,mating,egg-laying,and egg ***,loss of PERIOD disrupted the molecular rhythm of period and changed the clock transcription rhythm in the heads of the moths under LD and DD ***,our study indicates that the PERIOD is required for normal expression of many behavioral rhythms in ***.

关键词： CRISPR/Cas9 circadian rhythm period Plutella xylostella

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The receptor-like cytoplasmic kinase OsBSK1-2 regulates immunity via an HLH/bHLH complex

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Journal of Integrative Plant Biology 2024年第12期66卷 2754-2771页

作者： Xun Wang Zhijuan Diao Chang Cao Yan Liu Na Xia Youlian Zhang Ling Lu Fanyu Kong Houli Zhou Lizhe Chen Jing Zhang Bangsheng Wang Ronghua Huang Dingzhong Tang Shengping Li State Key Laboratory of ecological control of fujian-taiwan crop pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterCollege of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002China College of Agriculture Fujian Agriculture and Forestry UniversityFuzhou 350002China fujian Provincial Key Laboratory of crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002China

Plants need to fine-tune defense responses to maintain a robust but flexible host barrier to various ***-loop-helix/basic helix-loop-helix(HLH/bHLH)complexes play important roles in fine-tuning plant ***,the function of these genes in plant immunity and how they are regulated remain ***,we identified an atypical bHLH transcription factor,Oryza sativa(Os)HLH46,that interacts with rice receptor-like cytoplasmic kinase(RLCK)Os BRASSINOSTEROID-SIGNALING KINASE1-2(OsBSK1-2),which plays a key role in rice blast ***1-2 stabilized OsHLH46 both in vivo and in *** addition,OsHLH46 positively regulates rice blast resistance,which depends on ***46 has no transcriptional activation activity and interacts with a typical bHLH protein,OsbHLH6,which negatively regulates rice blast ***6 binds to the promoter of OsWRKY45 and inhibits its expression,while OsHLH46 suppresses the function of OsbHLH6 by blocking its DNA binding and transcriptional inhibition of *** with these findings,OsWRKY45 was up-regulated in OsHLH46-overexpressing *** addition,the oshlh46 mutant overexpressing OsbHLH6 is more susceptible to Magnaporthe oryzae than is the wild type,suggesting that OsHLH46 suppresses OsbHLH6-mediated rice blast *** results not only demonstrated that OsBSK1-2 regulates rice blast resistance via the OsHLH46/OsbHLH6 complex,but also uncovered a new mechanism for plants to fine-tune plant immunity by regulating the HLH/bHLH complex via RLCKs.

关键词： Magnaporthe oryzae OsbHLH6 OsBSK1-2 OsHLH46 rice rice blast resistance RLCK

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Electroantennogram and behavioral responses of Cotesia plutellae to plant volatiles

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Insect Science 2016年第2期23卷 245-252页

作者： Guang Yang You-Nan Zhang Geoff M. Gurr Liette Vasseur Min-Sheng You Institute of Applied Ecology Fujian Agriculture and Forestry University Fuzhou China fujian-taiwan joint centre for ecological control of crop pests Fujian Agriculture and Forestry University Fuzhou China Key Laboratory of Integrated Pest Management of fujian and taiwan China Ministry Agriculture Fuzhou Chin provincial Key Laboratory of Insect Ecology Fuzhou China Putian Institute of Agricultural Sciences Putian China Graham centre Charles Sturt University & NSW Department of Primary Industries Orange New South Wales Australia Department of Biological Sciences Brock University 500 Glenridge Avenue St. Catharines Ontario Canada

Plant volatiles have been demonstrated to play an important role in regulat- ing the behavior of Cotesia plutellae, a major larval parasitoid of the diamondback moth （DBM）, Plutella xylostella, but little is currently known about the function of each volatile and their mixtures. We selected 13 volatiles of the DBM host plant, a cruciferous veg- etable, to study the electroantennogram （EAG） and behavioral responses of C. plutellae. EAG responses to each of the compounds generally increased with concentration. Strong EAG responses were to 100 μL/mL of trans-2-hexenal, benzaldehyde, nonanal and cis-3- hexenol, and 10/zL/mL of trans-2-hexenal and benzaldehyde with the strongest response provoked by trans-2-hexenal at 100μL/mL. In the Y-tube olfactometer, C. plutellae, was significantly attracted by 1μL/mL of trans-2-hexenal and benzaldehyde.β-caryophyllene, cis-3-hexenol or trans-2-hexenal significantly attracted C. plutellae at 10μL/mL, while nonanal, benzyl alcohol, cis-3-hexenol or benzyl cyanide at 100μL/mL significantly at- tracted C. plutellae. Trans-2-hexenal significantly repelled C. plutellae at 100 μL/mL. EAG of C. plutellae showed strong responses to all mixtures made of five various com- pounds with mixtures 3 （trans-2-hexenal, benzaldehyde, nonanal, cis-3-hexenol, benzyl cyanide, farnesene, eucalyptol） and 4 （trans-2-hexenal, benzaldehyde, benzyl alcohol, （R）- （＋）-limonene,β-ionone, farnesene, eucalyptol） significantly attracting C. plutellae. These findings demonstrate that the behavior of C. plutellae can be affected either by individual compounds or mixtures of plant volatiles, suggesting a potential of using plant volatiles to improve the efficiency of this parasitoid for biocontrol ofP. xylostella.

关键词： behavioral response Cotesia plutellae electroantennogram response herbivore-induced plant volatile

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