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A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

The Crop Journal 2024年第2期12卷 569-582页

作者： Suting Wu Htin Kyaw Zhijun Tong Yirong Yang Zhiwei Wang Liying Zhang Lihua Deng Zhiguo Zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic *** available genome-editing tools have a limited number of targets,restricting their application in genetic *** this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software *** combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was *** plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

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T2-weighted imaging-based radiomic-clinical machine learning model for predicting the differentiation of colorectal adenocarcinoma

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 819-832页

作者： Hui-Da Zheng Qiao-Yi Huang Qi-Ming Huang Xiao-Ting Ke Kai Ye Shu Lin Jian-Hua Xu Department of Gastrointestinal Surgery The Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Department of Gynaecology and Obstetrics The Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Department of Computed Tomography/Magnetic Resonance Imaging The Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Centre of Neurological and Metabolic Research The Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Group of Neuroendocrinology Garvan Institute of Medical ResearchSydneyNSW 2010Australia

BACKGROUND The study on predicting the differentiation grade of colorectal cancer(CRC)based on magnetic resonance imaging(MRI)has not been reported *** a non-invasive model to predict the differentiation grade of CRC is of great *** To develop and validate machine learning-based models for predicting the differ-entiation grade of CRC based on T2-weighted images(T2WI).METHODS We retrospectively collected the preoperative imaging and clinical data of 315 patients with CRC who underwent surgery from March 2018 to July *** were randomly assigned to a training cohort(n=220)or a validation cohort(n=95)at a 7:3 *** were delineated layer by layer on high-resolution *** absolute shrinkage and selection operator regression was applied to screen for radiomic *** and clinical models were constructed using the multilayer perceptron(MLP)*** radiomic features and clinically relevant variables(selected based on a significance level of P<0.05 in the training set)were used to construct radiomics-clinical *** performance of the three models(clinical,radiomic,and radiomic-clinical model)were evaluated using the area under the curve(AUC),calibration curve and decision curve analysis(DCA).RESULTS After feature selection,eight radiomic features were retained from the initial 1781 features to construct the radiomic *** different classifiers,including logistic regression,support vector machine,k-nearest neighbours,random forest,extreme trees,extreme gradient boosting,light gradient boosting machine,and MLP,were used to construct the model,with MLP demonstrating the best diagnostic *** AUC of the radiomic-clinical model was 0.862(95%CI:0.796-0.927)in the training cohort and 0.761(95%CI:0.635-0.887)in the validation *** AUC for the radiomic model was 0.796(95%CI:0.723-0.869)in the training cohort and 0.735(95%CI:0.604-0.866)in the validation *** clinical model achieved an AUC of 0.7

关键词： Radiomics Colorectal cancer Differentiation grade Machine learning T2-weighted imaging

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Non-destructive electroluminescence inspection for LED epitaxial wafers based on soft single-contact operation

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Photonics Research 2024年第8期12卷 1776-1784页

作者： HAO SU JIAWEN QIU JUNLONG LI RONG CHEN JIANBI LE XIAOYANG LEI YONGAI ZHANG XIONGTU ZHOU TAILIANG GUO CHAOXING WU School of Physics and Information Engineering Fuzhou UniversityFuzhou 350000China fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China Fuzhou 350108China fujian Inspection and Research institute for Product Quality Fuzhou 350002China

Non-destructive and accurate inspection of gallium nitride light-emitting diode(Ga N-LED)epitaxial wafers is important to Ga N-LED ***,the conventional electroluminescence inspection,the photoluminescence inspection,and the automated optical inspection cannot fulfill the complex technical *** this work,an inspection method and an operation system based on soft single-contact operation,namely,single-contact electroluminescence(SC-EL)inspection,are *** key component of the SC-EL inspection system is a soft conductive probe with an optical fiber inside,and an AC voltage(70Vpp,100 k Hz)is applied between the probe and the ITO electrode under the LED epitaxial *** proposed SC-EL inspection can measure both the electrical and optical parameters of the LED epitaxial wafer at the same time,while not causing mechanical damage to the LED epitaxial ***,it is demonstrated that the SC-EL inspection has a higher electroluminescence wavelength accuracy than photoluminescence *** results show that the non-uniformity of SC-EL inspection is 444.64%,which is much lower than that of photoluminescence *** addition,the obtained electrical parameters from SC-EL can reflect the reverse leakage current(Is)level of the LED epitaxial *** proposed SC-EL inspection can ensure high inspection accuracy without causing damage to the LED epitaxial wafer,which holds promising application in LED technology.

关键词： electroluminescence operation epitaxial

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Online soft measurement for wastewater treatment system based on hybrid deep learning

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Frontiers of Environmental Science & Engineering 2024年第2期18卷 77-89页

作者： Wenjie Mai Zhenguo Chen Xiaoyong Li Xiaohui Yi Yingzhong Zhao Xinzhong He Xiang Xu Mingzhi Huang SCNU Environmental Research institute Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety&MOE Key Laboratory of Theoretical Chemistry of EnvironmentSchool of EnvironmentSouth China Normal UniversityGuangzhou 510006China fujian Environmental Protection design institute Co.Ltd. Fuzhou 350000China Huashi(fujian)Environment Technology Co.Ltd. Quanzhou362001China SCNU Qingyuan institute of Science and Technology Innovation Co.Ltd. Qingyuan 511517China Econ Technology Co.Ltd. Yantai 265503China

The existing automated wastewater treatment control systems encounter challenges such as the utilization of specialized testing instruments, equipment repair complications, high operational costs, substantial operational errors, and low detection accuracy. An effective soft measure model offers a viable approach for real-time monitoring and the development of automated control in the wastewater treatment process. Consequently, a novel hybrid deep learning CNN-BNLSTM-Attention (CBNLSMA) model, which incorporates convolutional neural networks (CNN), bidirectional nested long and short-term memory neural networks (BNLSTM), attention mechanisms (AM), and Tree-structure Parzen Estimators (TPE), has been developed for monitoring effluent water quality during the wastewater treatment process. The CBNLSMA model is divided into four stages: the CNN module for feature extraction and data filtering to expedite operations;the BNLSTM module for temporal data’s temporal information extraction;the AM module for model weight reassignment;and the TPE optimization algorithm for the CBNLSMA model’s hyperparameter search optimization. In comparison with other models (TPE-CNN-BNLSTM, TPE-BNLSTM-AM, TPE-CNN-AM, PSO-CBNLSTMA), the CBNLSMA model reduced the RMSE for effluent COD prediction by 25.4%, decreased the MAPE by 32.9%, and enhanced the R2 by 14.9%. For the effluent SS prediction, the CBNLSMA model reduced the RMSE by 26.4%, the MAPE by 21.0%, and improved the R2 by 35.7% compared to other models. The simulation results demonstrate that the proposed CBNLSMA model holds significant potential for real-time effluent quality monitoring, indicating its high potential for automated control in wastewater treatment processes.

关键词： Prediction model Soft measurement CNN-BNLSTM-AM model TPE optimization algorithm

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Introducing hydroxyl groups to tailor the d-band center of Ir atom through side anchoring for boosted ORR and HER

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Journal of Energy Chemistry 2024年第3期90卷 144-151,I0005页

作者： Qing Lv Meiping Li Xiaodong Li Xingru Yan Zhufeng Hou Changshui Huang Science Center for Material Creation and Energy Conversion School of Chemistry and Chemical EngineeringInstitute of Frontier and Interdisciplinary ScienceShandong UniversityJinan 250100ShandongChina institute of Chemistry Chinese Academy of ScienceBeijing 100191China State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002FujianChina

Tuning the coordination atoms of central metal is an effective means to improve the electrocatalytic activity of atomic ***,iridium(Ir) is proposed to be asymmetrically anchored by sp-N and pyridinic N of hydrogen-substituted graphdiyne(HsGDY),and coordinated with OH as an Ir atomic catalyst(Ir_(1)-N-HsGDY).The electron structures,especially the d-band center of Ir atom,are optimized by these specific coordination ***,the as-synthesized Ir_(1)-N-HsGDY exhibits excellent electrocatalytic performances for oxygen reduction and hydrogen evolution reactions in both acidic and alkaline *** from the unique structure of HsGDY,IrN_(2)(OH)_(3) has been developed and demonstrated to act as the active site in these electrochemical *** those indicate the fresh role of the sp-N in graphdiyne in producing a new anchor way and contributing to promote the electrocatalytic activity,showing a new strategy to design novel electrochemical catalysts.

关键词： Oxygen reduction reaction D-band center Graphdiyne Hydroxyl group Electrocatalysis

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Vibration response and safety control for blasting vibration of the existing tunnel with defects

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Underground Space 2024年第2期15卷 76-89页

作者： Junying Xia Bo Wang Xinxin Guo Zhuoxiong Xie Key Laboratory of Transportation Tunnel Engineering Ministry of EducationSouthwest Jiaotong UniversityChengdu 610031China College of Environment and Civil Engineering Chengdu University of TechnologyChengdu 610059China Guangdong Communication planning&design institute Group Co. LtdGuangzhou 510507China

Current studies on blasting construction of small clear-distance tunnels have not considered the impact of existing tunnel lining defects when establishing safety *** paper offers a series of study results based on the blasting project of a new tunnel adjacent to the existing defect Xinling tunnel to thoroughly examine the dynamic response,safety control standards,and measures of the existing defect ***,structural models were developed to investigate the influence of the presence or absence of specific defects(like lining cracks and cavities behind the lining)on the dynamic response of the current tunnel lining to identify the most unfavorable defect ***,establish safety control standards for intact linings and those with the most unfavorable ***,two types of control measures,single safe charge and reasonable delay time,were studied based on the established safety control *** particular,the most adverse position of cracks was the wall facing the explosion,the rise in depth was more unfavorable for vibration response,and the impact of the longitudinal crack was restricted to the vicinity of the *** the vault was the most adverse cavity position,the rise in cavity area was more damaging,and the influence range varied with longitudinal cavity ***,the impact of cracks was mainly evident in the amplification effect of stress at the crack *** contrast,cavities had varied degrees of amplification effects on the vibration velocity and stress response and a relatively extensive influence *** control research was conducted,when the tunnel was intact,with a right wall crack,a vault cavity,and both vault cavity and crack for this project,the peak particle velocity(PPV)of the safety control standard for vibration velocity was 13,10,13,and 8 cm/s,respectively,and the respective single safe charge could be adjusted at 64,53,37,and 25 ***,the presence of different defects had a relati

关键词： Existing tunnel Defect Blasting construction Vibration response Control standards and measures

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Equivalent Impedance Parameter Calculation of Three-phase Symmetrical Loads for Harmonic Source Location

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Journal of Modern Power Systems and Clean Energy 2024年第1期12卷 251-260页

作者： Yi Zhang Bijie Liu Caihua Lin Zhenguo Shao Yuncong Xu the Department of Electrical Engineering and Automation Fuzhou UniversityFuzhouChina State Grid fujian Electric Power Research institute FuzhouChina

The equivalent impedance parameters of loads have been widely used to identify and locate the harmonic ***,the existing calculation methods suffer from outliers caused by the zero-crossing of the *** outliers can result in inaccuracy and unreliability of harmonic source *** address this issue,this paper proposes an innovative method of equivalent impedance parameter calculation of three-phase symmetrical loads that avoid *** correctness and effectiveness of the proposed method are verified by simulations on Simulink using actual monitoring *** results show that the proposed method is not only simple and easy to implement but also highly accurate.

关键词： abc-αβtransformation equivalent impedance parameters of load harmonic source location nonlinear load power quality

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Agricultural intensity for sustainable regional development:A case study in peri-urban areas of Karawang Regency,Indonesia

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Regional Sustainability 2024年第1期5卷 119-136页

作者： Setyardi Pratika MULYA Delik HUDALAH urban and Regional planning Study Program School of ArchitecturePlanningand Policy DevelopmentBandung Institute of TechnologyBandung40132Indonesia Department of Soil Science and Land Resources Faculty of AgricultureBogor Agricultural UniversityBogor16680Indonesia Research Center for Infrastructure and Regional Development Bandung Institute of TechnologyBandung40132Indonesia

Peri-urban areas are playing an increasingly crucial role in the agricultural development and intensification in ***-urban agriculture is highly vulnerable to change compared with urban and rural agriculture,due to its location in transitional *** of peri-urban agricultural intensity can help guide regional *** this study,we assessed the sustainability of peri-urban areas based on agricultural intensity in Karawang Regency,*** developed a village-based index to assess the region’s agricultural intensity by rescaling the village agriculture index(VAI)and combining the local sustainability index(LSI)with factor *** the unit of analysis is the village,we modified the LSI to the village sustainability index(VSI).In addition,we also developed a logical matrix analysis to determine the level of agricultural sustainability(LoAS)of each *** combined results of the three indices(VAI,VSI,and LoAS)generated information about agricultural *** results indicated that peri-urban villages with high agricultural intensity tended to exhibit low levels of social welfare,economic development,and disaster ***,high agricultural intensity did not necessarily ensure the prosperity of the ***,there was the economic disparity among the villages in the study *** diversity of agricultural intensity seems to be more critical than promoting agricultural intensity ***,this study highlights the distinctive characteristics and dynamic of peri-urban *** approaches,variables,and information regarding the combination of agricultural intensity and sustainability need to be developed as valuable tools for regional planning.

关键词： Agricultural intensity Village agriculture index(VAI) Village sustainability index(VSI) Level of agricultural sustainability(LoAS) Peri-urban areas Indonesia

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Impact and prediction of pollutant on mangrove and carbon stocks:A machine learning study based on urban remote sensing data

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Geoscience Frontiers 2024年第3期15卷 401-418页

作者： Mengjie Xu Chuanwang Sun Yanhong Zhan Ye Liu institute of Artificial Intelligence Xiamen UniversityXiamen 361005FujianChina China Center for Energy Economics Research School of EconomicsXiamen UniversityXiamen 361005FujianChina Laboratory of Smart Energy and Green Economy Institute of Artificial IntelligenceXiamen UniversityXiamen 361005FujianChina Department of Public Finance School of EconomicsXiamen UniversityXiamen 361005China

Mangrove ecosystems have important ecological and economic values,especially their ability to store ***,in recent years,human disturbance has accelerated mangrove *** them,the emission of pollutants cannot be *** is of great significance for carbon emission reduction and ecological protection to study the impacts of different pollutants on mangroves and their carbon *** on the remote sensing data of coastal areas south of the Yangtze River in China's Mainland,this paper builds the ensemble learning model Random Forest(RF)and Gradient Boosting Regression(GBR)to empirically analyse the relationship between industrial wastewater,industrial sulfur dioxide(SO2),PM2.5 and mangrove *** results show that the pollutant concentration of meteorological normalisation is more *** importance of pollutants presents regional *** area of mangroves in different cities and the corresponding total carbon stocks show different trends with the increase or decrease of pollutants,and there is a dynamic balance between urban pollutant discharge and mangrove growth in some *** research in this paper provides an analysis and explanation from the perspective of machine learning to explore the relationship between mangroves and pollutants and at the same time,provides scientific suggestions for the formulation of future pollutant emission policies in different cities.

关键词： Mangrove forests Pollutants Machine learning model Carbon stocks Regional heterogeneity

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LATS1 Promotes B-ALL Tumorigenesis by Regulating YAP1 Phosphorylation and Subcellular Localization

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Current Medical Science 2024年第1期44卷 81-92页

作者： Feng ZHANG Mohammed Awal Issah Hai-ying FU Hua-rong ZHOU Ting-bo LIU Jian-zhen SHEN fujian Provincial Key Laboratory on Hematology Fujian Medical Center of HematologyFujian Institute of HematologyClinical Research Center for Hematological Malignancies of Fujian ProvinceFujian Medical University Union HospitalFuzhou 350001China Tamale Technical University Faculty of Allied Health and Pharmaceutical SciencesDepartment of Medical Laboratory TechnologyTamale NS-011-2000Ghana Department of Hematology The Third Affiliated People's Hospital of Fujian University of Traditional Chinese MedicineThe Third People's Hospital of Fujian ProvinceFuzhou 350122China

Objective YAP1 plays a dual role as an oncogene and tumor suppressor gene in several tumors;differentiating between these roles may depend on the YAP1 phosphorylation *** specific function of YAP1 in B cell acute lymphoblastic leukemia(B-ALL),however,is currently ***,in the present study,the role of YAP1 in B-ALL was investigated using relevant cell lines and patient *** The effects of shRNA-mediated knockdown on YAP1 and LATS1 levels in the NALM6 and MOLT-4 cell lines were examined using Western blotting,quantitative real-time polymerase chain reaction,flow cytometry,immunostaining,and nude mouse subcutaneous tumorigenesis *** expression levels of Hippo pathway-related molecules before and after verteporfin(VP)treatment were compared using RNA-Seq to identify significant Hippo pathway-related genes in NALM6 *** Patients with ALL showing high YAP1 expression and low YAP1-Ser127 phosphorylation levels had worse prognoses than those with low YAP1 protein expression and high YAP1-Ser127 phosphorylation ***1-Ser127 phosphorylation levels were lower in NALM6 cells than in MOLT-4 and control cells;YAP1 was distributed in the nuclei in NALM6 *** of YAP1 inhibited MOLT-4 and NALM6 cell proliferation and arrested the NALM6 cell cycle in the G0/G1 *** and after VP treatment,the expression of the upstream gene LATS1 was upregulated;its overexpression promoted YAP1-Ser127 ***,YAP1 was distributed in the *** LATS1 may downregulate YAP1-Ser127 phosphorylation and maintain B-ALL cell function;thus,VP,which targets this axis,may serve as a new therapeutic method for improving the outcomes for B-ALL patients.

关键词： acute lymphoblastic leukemia large tumor suppressor kinase 1 phosphorylation RNA-Seq Yesl-associated protein

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