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Host-induced gene silencing of multiple pathogenic factors of Sclerotinia sclerotiorum confers resistance to Sclerotinia rot in Brassica napus

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The Crop Journal 2022年第3期10卷 661-671页

作者： Jian Wu Shengliang Yin Li Lin Dongxiao Liu Sichao Ren Wenjing Zhang Wencheng Meng Peipei Chen Qinfu Sun Yujie Fang Cunxu Wei Youping Wang key laboratory of plant functional genomics of the Ministry of Education Yangzhou UniversityYangzhouChina Jiangsu key laboratory of Crop genomics and Molecular Breeding Yangzhou UniversityYangzhouChina

Sclerotinia sclerotiorum is generally considered one of the most economically damaging pathogens in oilseed rape(Brassica napus).Breeding for Sclerotinia resistance is challenging,as no immune germplasm available in *** is desirable to develop new breeding *** the present study,hostinduced gene silencing(HIGS),developed based on RNA interference(RNAi),was applied to protect *** from *** *** pathogenicity genes,the endo-polygalacturonase gene(Ss PG1),cellobiohydrolase gene(Ss CBH),and oxaloacetate acetylhydrolase gene(Ss OAH1),were chosen as HIGS ***-incubation of synthesized double-stranded RNAs(ds RNAs)with *** in liquid medium significantly reduced the transcript levels of the target *** to plant surfaces of ds RNA targeting the three genes conferred effective protection against *** transgenic *** plants expressing small interfering RNAs with sequence identity to Ss PG1,Ss CBH,and Ss OAH1 were *** transgenic *** prevented the expression of *** target genes,slowed pathogenicity-factor accumulation,impeded fungal growth,and suppressed appressorium formation,thereby conferring resistance to *** silencing of Ss PG1,Ss CBH,and Ss OAH1 by stable expression of a chimeric hairpin RNAi construct in *** led to enhanced protection phenotypes(with disease lesion size reduced by 36.8%–43.7%).We conclude that HIGS of pathogenic-factor genes of *** is a promising strategy for controlling Sclerotinia rot in oilseed rape.

关键词： Sclerotinia sclerotiorum Brassica napus SIGS HIGS RNAi

plant immune signaling: Advancing on two frontiers

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Journal of Integrative plant Biology 2020年第1期62卷 2-24页

作者： Wei Wang Baomin Feng Jian-Min Zhou Dingzhong Tang State key laboratory of Ecological Control of fujian-Taiwan Crop Pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou 350002China The State key laboratory of plant genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

plants have evolved multiple defense strategies to cope with pathogens, among which plant immune signaling that relies on cell-surface localized and intracellular receptors takes fundamental roles. Exciting breakthroughs were made recently on the signaling mechanisms of pattern recognition receptors(PRRs) and intracellular nucleotide-binding site(NBS) and leucine-rich repeat(LRR)domain receptors(NLRs). This review summarizes the current view of PRRs activation, emphasizing the most recent discoveries about PRRs’ dynamic regulation and signaling mechanisms directly leading to downstream molecular events including mitogen-activated protein kinase(MAPK) activation and calcium(Ca2+) burst. plants also have evolved intracellular NLRs to perceive the presence of specific pathogen effectors and trigger more robust immune responses. We also discuss the current understanding of the mechanisms of NLR activation, which has been greatly advanced by recent breakthroughs including structures of the first full-length plant NLR complex, findings of NLR sensor-helper pairs and novel biochemical activity of Toll/interleukin-1 receptor(TIR) domain.

关键词： breakthrough activation frontier

Crop antiviral defense: Past and future perspective

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Science China Life Sciences 2024年第12期67卷 2617-2634页

作者： Zhirui Yang Guangyao Li Yongliang Zhang Fangfang Li Tao Zhou Jian Ye Xianbing Wang Xiaoming Zhang Zongtao Sun Xiaorong Tao Ming Wu Jianguo Wu Yi Li The State key laboratory of Protein and plant Gene Research School of Life Sciences Peking University State key laboratory of plant Environmental Resilience Frontiers Science Center for Molecular Design Breeding College of Biological Sciences China Agricultural University State key laboratory for Ecological Pest Control of fujian and Taiwan Crops Vector-borne Virus Research Center College of Plant Protection Fujian Agriculture and Forestry University State key laboratory for Biology of plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences State key laboratory for Agro-Biotechnology and Department of plant Pathology China Agricultural University State key laboratory of plant genomics Institute of Microbiology Chinese Academy of Sciences State key laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences CAS Center for Excellence in Biotic Interactions University of Chinese Academy of Sciences State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province Institute of Plant Virology Ningbo University Department of plant Pathology The Key Laboratory of Plant Immunity Nanjing Agricultural University

Viral pathogens not only threaten the health and life of humans and animals but also cause enormous crop yield losses and contribute to global food insecurity. To defend against viral pathogens, plants have evolved an intricate immune system to perceive and cope with such attacks. Although most of the fundamental studies were carried out in model plants, more recent research in crops has provided new insights into the antiviral strategies employed by crop plants. We summarize recent advances in understanding the biological roles of cellular receptors, RNA silencing, RNA decay, hormone signaling, autophagy, and ubiquitination in manipulating crop host-mediated antiviral responses. The potential functions of circular RNAs, the rhizosphere microbiome, and the foliar microbiome of crops in plant-virus interactions will be fascinating research directions in the future. These findings will be beneficial for the development of modern crop improvement strategies.

关键词： crops antiviral defense RNA silencing autophagy ubiquitin proteasome system

New insights onthe Magnaportheoryzaegenome structure by Pac Bio sequencing

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New insights onthe Magnaportheoryzaegenome structure by Pac ...

Changes in the epigenome and transcriptome of rice in response to Magnaporthe oryzae infection

中国菌物学会2016年学术年会

作者： Jiandong Bao Zhenhui Zhong Meilian Chen Lianyu Lin Wei Tang Justice Norvienyeku Baohua Wang Guodong Lu Zonghua Wang fujian-Taiwan Joint Center for Ecological Control of Crop Pests Fujian Agriculture and Forestry University fujian Province key laboratory of Pathogenic Fungiand Mycotoxins Fujian Agriculture and Forestry University fujian university key laboratory for functional genomics of plant fungal pathogens Fujian Agricultureand Forestry University

＜正＞Magnaportheoryzae,is a kind of filamentous ascomycetefungi that causes the most serious infectious disease of cultivated ***-generation-sequencing（NGS）has emerged as an efficient,reliable and the most economical technology for rapid genome *** NGS technology has been successfully deployed to generate genome sequence data for 53 isolates of the fungus,and corresponding results could be freely obtained from NCBI *** provide good opportunity to

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The Crop Journal 2021年第4期9卷 843-853页

作者： Na Cui Xiaofeng Chen Yan Shi Meirong Chi Jiantuan Hu Kunlong Lai Zonghua Wang Haifeng Wang State key laboratory of Ecological Pest Control for fujian and Taiwan Crops College of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Marine and Agricultural Biotechnology laboratory Institute of OceanographyMinjiang UniversityFuzhou 350108FujianChina fujian Province key laboratory of Pathogenic Fungi and Mycotoxins Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina State key laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Key Lab of Sugarcane BiologyCollege of AgricultureGuangxi UniversityNanning 530004GuangxiChina

DNA methylation participates in regulating the expression of coding and non-coding regions in plants. To investigate the association between DNA methylation and pathogen infection, we used whole-genome bisulfite sequencing to survey temporal DNA methylation changes in rice after infection with the rice blast fungus Magnaporthe oryzae. In contrast to previous findings in Arabidopsis, global DNA methylation levels in rice increased slightly after rice blast infection. We identified over 38,000 differentially methylated regions(DMRs), and hypermethylated DMRs far outnumbered hypomethylated DMRs. Most DMRs were located in transposable element regions. Using transcriptome analysis, we identified 8830 differentially expressed genes(DEGs) after 1, 3, and 5 days of infection. Over one-third of DEGs, most of which were CHH-type DMRs, were associated with DMRs. functional analysis of the CHH DMR-DEGs indicated their involvement in many important biological processes, including cell communication and response to external stimulus. The transcription of many NBS-LRR family genes was affected by changes in DNA methylation, suggesting that DNA methylation plays essential roles in the response of rice to M. oryzae infection. More broadly, the DNA methylation analysis presented here sheds light on epigenomic involvement in plant defense against fungal pathogens.

关键词： DNA methylation Epigenome Magnaporthe oryzae Rice Transcriptome

A Bunyavirus-Inducible Ubiquitin Ligase Targets RNA Polymerase IV for Degradation during Viral Pathogenesis in Rice

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Molecular plant 2020年第6期13卷 836-850页

作者： Chao Zhang Ying Wei Le Xu Kang-Cheng Wu Liang Yang Chao-Nan Shi Guo-Yi Yang Dong Chen Fei-Fei Yu Qi Xie Shou-Wei Ding Jian-Guo Wu Vector-borne Virus Research Center Key Laboratory of Plant Virology of Fujian ProvinceInstitute of Plant VirologyCollege of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002China Center for plant Biology Tsinghua-Peking Center for Life SciencesCollege of Life SciencesTsinghua UniversityBeijing 100084China State key laboratory of plant genomics National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Department of Microbiology and plant Pathology and Center for plant Cell Biology Institute for Integrative Genome BiologyUniversity of CaliforniaRiversideCA92521USA

The ubiquitin-proteasome system(UPS)is an important post-translational regulatory mechanism that controls many cellular functions in ***,we show that stable expression of P3 protein encoded by Rice grassy stunt virus(RGSV),a negative-strand RNA virus in the Bunyavirales,causes developmental abnormities similar to the disease symptoms caused by RGSV,such as dwarfing and excess tillering,in transgenic rice *** found that both transgenic expression of P3 and RGSV infection induce ubiquitination and UPS-dependent degradation of rice NUCLEAR RNA POLYMERASE D1a(OsNRPD1a),one of two orthologs of the largest subunit of plant-specific RNA polymerase IV(Pol IV),which is required for RNA-directed DNA methylation(RdDM).Furthermore,we identified a P3-inducible U-box type E3 ubiquitin ligase,designated as P3-inducible protein 1(P3IP1),which interacts with OsNRPD1a and mediates its ubiquitination and UPS-dependent degradation in vitro and in ***,both knockdown of OsNRPD1 and overexpression of P3IP1 in rice plants induced developmental phenotypes similar to RGSV disease *** together,our findings reveal a novel virulence mechanism whereby plant pathogens target host RNA Pol IV for UPS-dependent degradation to induce disease *** study also identified an E3 ubiquitin ligase,which targets the RdDM compotent NRPD1 for UPS-mediated degradation in rice.

关键词： ubiquitin-proteasome system NRPD1 Rice grassy stunt virus E3 ligase

RECEPTOR-LIKE KINASE 902 Associates with and Phosphorylates BRASSINOSTEROID-SIGNALING KINASE1 to Regulate plant Immunity

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Molecular plant 2019年第1期12卷 59-70页

作者： Yaofei Zhao Guangheng Wu Hua Shi Dingzhong Tang State key laboratory of Ecological Control of fujian-Taiwan Crop Pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhouFujian 350002China university of Chinese Academy of Sciences Beijing 100039China Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China Provincial key laboratory of Eco-lndustrial Green Technology College of Ecology and Resources EngineeringWuyi UniversityWuyishanFujian 354300China fujian key laboratory of Crop by Design Fujian Agriculture and Forestry UniversityFuzhou 350002China

plants employ receptor-like kinases (RLKs)and receptor-like proteins for rapid recognition of invading pathogens,and RLKs then transmit signals to receptor-like cytoplasmic kinases (RLCKs)to activate immune *** are under fine regulation mediated by subcellular trafficking,which contributes to proper activation of plant *** this study,we show that Arabidopsisthaliana RECEPTOR-LIKE KINASE 902 (RLK902)plays important roles in resistance to the bacterial pathogen Pseudomonas syringae, but not to the fungal powdery mildew pathogen Golovinomyces ***902 localizes at the plasma membrane and associates with ENHANCED DISEASE RESISTANCE 4 (EDR4),a protein involved in clathrin-mediated trafficking ***4 and CLATHRIN HEAVY CHAIN 2 (CHC2)regulate the subcellular trafficking and accumulation of RLK902 ***,we found that RLKg02 directly associates with the RLCK BRASSINOSTEROID-SIGNALING KINASE1 (BSK1),a key component of plant immunity,but not with other members of the FLAGELLIN SENSING 2 immune ***902 phosphorylates BSK1,and its Ser-230 is a key phosphorylation site critical for RLK902-mediated defense signaling. Taken together,our data indicate that EDR4 regulates plant immunity by modulating the subcellular trafficking and protein accumulation of RLK902,and that RLK902 transmits immune signals by phosphorylating BSK1.

关键词： RLK902 BSK1 EDR4 subcellular trafficking phosphorylation plant immunity

plant virology in the 21st century in China:Recent advances and future directions

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Journal of Integrative plant Biology 2024年第3期66卷 579-622页

作者： Jianguo Wu Yongliang Zhang Fangfang Li Xiaoming Zhang Jian Ye Taiyun Wei Zhenghe Li Xiaorong Tao Feng Cui Xianbing Wang Lili Zhang Fei Yan Shifang Li Yule Liu Dawei Li Xueping Zhou Yi Li State key laboratory for Ecological Pest Control of fujian and Taiwan Crops Vector-borne Virus Research CenterCollege of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002China State key laboratory of plant Environmental Resilience and Ministry of Agriculture key laboratory of Soil Microbiology College of Biological SciencesChina Agricultural UniversityBeijing 100193China State key laboratory for Biology of plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State key laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China State key laboratory of plant genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China State key laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Department of plant Pathology The Key Laboratory of Plant ImmunityNanjing Agricultural UniversityNanjing 210095China State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant VirologyNingbo UniversityNingbo 315211China MOE key laboratory of Bioinformatics Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China State key laboratory of Protein and plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China

plant viruses are a group of intracellular pathogens that persistently threaten global food *** advances in plant virology have been achieved by Chinese scientists over the last 20 years,including basic research and technologies for preventing and controlling plant viral ***,we review these milestones and advances,including the identification of new crop-infecting viruses,dissection of pathogenic mechanisms of multiple viruses,examination of multilayered interactions among viruses,their host plants,and virus-transmitting arthropod vectors,and in-depth interrogation of plantencoded resistance and susceptibility ***,various plant virus-based vectors have also been successfully developed for gene function studies and target gene expression in *** also recommend future plant virology studies in China.

关键词： China emerging plant viruses plant virology plant resistance/susceptibility to viral infection plant-virus transmission vector interactions plant virus-based vectors viral pathogenesis

Finns-Kingdom RNA Silencing in plant-fungal Pathogen Interactions

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Molecular plant 2018年第2期11卷 235-244页

作者： Chenlei Hua Jian-Hua Zhao Hui-Shan Guo State key laboratory of plant genomics Institute of Microbiology Chinese Academy of Sciences 100101 Beijing China College of Life Science University of the Chinese Academy of Sciences 100049 Beijing China

fungal pathogens represent a major group of plant invaders that are the causative agents of many notorious plant diseases. Large quantities of RNAs, especially small RNAs involved in gene silencing, have been found to transmit bidirectionally between fungal pathogens and their hosts. Although host-induced gene silencing （HIGS） technology has been developed and applied to protect crops from fungal infections, the mechanisms of RNA transmission, especially small RNAs regulating trans- kingdom RNA silencing in plant immunity, are largely unknown. In this review, we summarize and discuss recent important findings regarding trans-kingdom sRNAs and RNA silencing in plant-fungal pathogen interactions compared with the well-known RNAi mechanisms in plants and fungi. We focus on the interactions between plant and fungal pathogens with broad hosts, represented by the vascular pathogen Verticillium dahliae and non-vascular pathogen Botrytis cinerea, and discuss the known instances of natural RNAi transmission between fungal pathogens and host plants. Given that HIGS has been developed and recently applied in controlling Verticillium wilt diseases, we propose an ideal research system exploiting plant vasculature-Verticillium interaction to further study trans-kingdom RNA silencing.

关键词： fungal pathogens HIGS microRNA trans-kingdom RNAi small RNA Verticillium dahliae