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The reference genome of seed hemp(Cannabis sativa)provides new insights into fatty acid and vitamin E synthesis

Plant Communications 2024年第1期5卷 12-15页

作者： Huawei Wei Zuqing Yang Sylvain Niyitanga Aifen Tao Jiantang Xu Pingping Fang Lihui Lin Liemei Zhang Jianmin Qi Ray Ming Liwu Zhang key laboratory of Ministry of Education for Genetic breeding and Multiple Utilization of crops/key laboratory of Ministry of Agriculture and Rural Affairs for Biological breeding of fujian and Taiwan crops Fujian Agriculture and Forestry UniversityFuzhou 350002China fujian public platform for germplasm resources of bast fiber crops/fujian provincial key laboratory of crop breeding by design Fujian Agriculture and Forestry UniversityFuzhou 350002China Center for Genomics and Biotechnology Fujian Agriculture and Forestry UniversityFuzhou 350002China

Dear Editor,Hemp(Cannabis sativa L.)(2n=20)is an annual herb of the Cannabaceae family,mostly dioecious and occasionally monoecious(Van et al.,2011;Long et al.,2017).A major source of food,textiles,and oilseed,it is believed to have originated in central Asia and spread rapidly throughout Asia and Europe(Andre et al.,2016;Braich et al.,2019;Gao et al.,2020).Depending on its usage and cannabinoid content,cannabis is classified as fiber type(hemp or industrial hemp)or drug type(medicinal cannabis or marijuana)(Struik et al.,2000;Hurgobin et al.,2021).As a type of industrial hemp,seed hemp is widely used to produce seed oil,but we still know little about the genetic basis of its fatty acid and vitamin E synthesis.

关键词： synthesis. fiber synthesis

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Development of an Agrobacterium-mediated CRISPR/Cas9 gene editing system in jute(Corchorus capsularis)

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The crop Journal 2024年第4期12卷 1266-1270页

作者： Shaolian Jiang Qin Li Xiangxue Meng Mengxin Huang Jiayu Yao Chuanyu Wang Pingping Fang Aifen Tao Jiantang Xu Jianmin Qi Shuangxia Jin Liwu Zhang key laboratory of Ministry of Education for Genetic breeding and Multiple Utilization of crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Experiment Station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/fujian public platform for germplasm resources of bast fiber crops/fujian provincial key laboratory of crop breeding by design/key laboratory of Ministry of Agriculture and Rural Affairs for Biological breeding of fujian and Taiwan crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National key Lab of crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after ***,developing an efficient gene editing system for jute remains a *** this study,the transgenic hairy root system mediated by Agrobacterium rhizogenes strain K599 was developed for Meifeng 4,an elite jute variety widely cultivated in *** transgenic hairy root system for jute was verified by subcellular localization and bimolecular fluorescence complementation(BiFC)*** CHLOROPLASTOS ALTERADOS 1(CcCLA1)gene,which is involved in the development of chloroplasts,was targeted for editing at two sites in Meifeng *** on this hairy root transformation,the gRNA scaffold was placed under the control of cotton ubiquitin GhU6.7 and-GhU6.9 promoters,respectively,to assess the efficiency of gene *** indicated the 50.0%(GhU6.7)and 38.5%(GhU6.9)editing events in the target 2 alleles(gRNA2),but no mutation was detected in the target 1 allele(gRNA1)in transgenic-positive hairy ***1 gene editing at gRNA2 under the control of GhU6.7 in Meifeng 4 was also carried out by Agrobacterium tumefaciens-mediated *** CcCLA1 mutants were albinic,with a gene editing efficiency of 5.3%.These findings confirm that the CRISPR/Cas9 system,incorporating promoter GhU6.7,can be used as a gene editing tool for jute.

关键词： Jute Agrobacterium-mediated transformation Genome editing Hairy root system

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De novo assembly of chloroplast genomes of Corchorus capsularis and *** yields species-specific InDel markers

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The crop Journal 2021年第1期9卷 216-226页

作者： Shusheng Fang Liemei Zhang Jianmin Qi Liwu Zhang key laboratory of Ministry of Education for Genetics Breeding and Multiple Utilization of Crops/Fujian Provincial Key Laboratory of Crop Breeding by DesignCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Experiment Station of Agriculture and Rural Affairs of Ministry for Jute and Kenaf in Southeast China/fujian public platform for germplasm resources of bast fiber crops/fujian International Science and Technology Cooperation Base for Genetics Breeding and Multiple Utilization Development of Southern Economic CropsFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Center for Genomics and Biotechnology Haixia Institute of Science and TechnologyFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina

Jute(Corchorus spp.)is a member of the Malvaceae family,which comprises more than 100 *** systematic positions of jute species have remained *** are maternally inherited and their genomes are widely used for plant phylogenetic *** the present study,the chloroplast genomes of Corchorus capsularis and *** were assembled,with sizes of respectively 161,088 and 161,766 *** genomes contained 112 unique genes(78 protein-coding,four rRNA,and 30 tRNA genes).Four regionswith high variation between the two species were located in single-copy rather than inverted-repeat regions.A total of 66 simple sequence repeats(SSRs)were identified in the *** chloroplast genome and 56 in that of *** of the two chloroplast genome sequences permitted the evaluation of nucleotide variation including 2417 single-nucleotide polymorphisms sites and 294 insertion or deletion sites,of which one marker(cpInDel 205)could discriminate the two jute *** of the *** and *** chloroplast genomeswith those of other species in theMalvaceae revealed breakpoints in the accD locus,which is involved in fatty acid synthesis,in *** and *** finding suggests that genes from the chloroplast genomemight have been transferred to the nuclear genome in some Corchorus *** hypothesis was supported by synteny analysis of the accD region among the nuclear,chloroplast,and mitochondrial *** our knowledge,this is the first report of the assembled chloroplast genome sequences of *** and *** and *** are closely related to Gossypium species and there are abundant microstructure variations between these two *** results will expand our understanding of the systematics of species in the Malvaceae.

关键词： Jute Corchorus Genome Chloroplast Insertion or deletion

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Genetic diversity and DNA fingerprinting in jute(Corchorus spp.) based on SSR markers

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The crop Journal 2015年第5期3卷 416-422页

作者： Liwu Zhang Rongrong Cai Minhang Yuan Aifen Tao Jiantang Xu Lihui Lin Pingping Fang Jianmin Qi College of crop Science Fujian Agriculture and Forestry University/Key Laboratory of Ministry of Education for Genetics Breeding and Multiple Utilization of Crops/Fujian Provincial Key Laboratory of Crop Breeding by Design

Genetic diversity analysis and DNA finger printing are very useful in breeding programs,seed conservation and management. Jute(Corchorus spp.) is the second most important natural fiber crop after cotton. DNA fingerprinting studies in jute using SSR markers are limited. In this study, 58 jute accessions, including two control varieties(Huangma 179 and Kuanyechangguo) from the official variety registry in China were evaluated with 28 pairs of SSR primers. A total of 184 polymorphic loci were identified. Each primer detected 3 to 15 polymorphic loci, with an average of 6.6. The 58 jute accessions were DNA-fingerprinted with 67 SSR markers from the 28 primer pairs. These markers differentiated the 58 jute accessions from one another, with Co SSR305-120 and Co SSR174-195 differentiating Huangma 179 and Kuanyechangguo, respectively. NTSYS-pc2.10 software was used to analyze the genetic diversity in the 58 jute accessions. Their genetic similarity coefficients ranged from 0.520 to 0.910 with an average of 0.749, indicating relatively great genetic diversity among them. The 58 jute accessions were divided into four groups with the coefficient 0.710 used as a value for classification, consistent with their species and pedigrees. All these results may be useful both for protection of intellectual property rights of jute accessions and for jute improvement.

关键词： Jute Corchorus Simple sequence repeats DNA fingerp

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