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Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella

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Journal of Integrative Agriculture 2024年第9期 3089-3103页

作者： Shanyu Li Guifang Lin Haoqi Wen Haiyan Lu Anyuan Yin Chanqin Zheng Feifei Li Qingxuan Qiao Lu Jiao Ling Lin Yi Yan Xiujuan Xiang Huang Liao Huiting Feng Yussuf Mohamed Salum Minsheng You Wei Chen Weiyi He State key laboratory for Ecological Pest Control of fujian and Taiwan Crops/Ministerial and provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops/International Joint research laboratory of Ecological Pest Control Ministry of EducationInstitute of Applied EcologyFujian Agriculture and Forestry University Ganzhou key laboratory of Greenhouse Vegetable Gannan Normal University

Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing. In the current study, we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella, in addition to its known functions in the ovarian development in female adults and egg hatching. Subsequent CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP) gene into autosomal Pxfl(2)d for labelling mutants could be achieved. However, this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive capacity. We further screened for the W-chromosomelinked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/Cas9. The results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous sequences. We ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP) expression cassettes on a Z-linked region in P. xylostella, which can also be used for early sex detection. By injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain, the gene editing efficiency reached 29.73%, confirming the success of expressing a functional Cas9 gene. Taken together, we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in P. xylostella, while the establishment of a heritable strain required the positioning of appropriate sites. This study provides an important working basis and technical support for further developing genetic strategies for insect pest control.

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Cu-catalyzed regioselective diborylation of 1,3-enynes for the efficient synthesis of 1,4-diborylated allenes

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Chinese Chemical Letters 2024年第5期35卷 141-144页

作者： Wujun Jian Mong-Feng Chiou Yajun Li Hongli Bao Song Yang National key laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyang 550025China Strait Institute of Flexible Electronics(SIFE Future Technologies)Fujian Normal UniversityFuzhou 350117China key laboratory of Coal to Ethylene Glycol and Its Related Technology State Key Laboratory of Structural ChemistryFujian Institute of Research on the Structure of MatterUniversity of Chinese Academy of SciencesFuzhou 350002China

Borylation of 1,3-enynes with bis(boronate)compounds often ends up with the formation of hydroborylated products,leaving the diborylation of 1,3-enynes for the formation of 1,4-diborylated allenes to be ***,a copper-catalyzed chemo-,regio-,and stereo-selective diborylation of 1,3-enynes for the efficient construction of 1,4-diborylated allenes under base-free conditions was reported.A wide range of 1,3-enynes bearing various functional groups can participant in the reaction and afforded the corresponding 1,4-diborylated allenes in good to excellent yields,which was enabled by the protocol of Bpin to BF3K *** borylcopper species was supposed to selectively attack the C-C triple bond of the 1,3-enynes.

关键词： Copper catalysis Regioselective 1,3-Enynes Borylation Allenes

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Pien Tze Huang Inhibits Migration and Invasion of Hepatocellular Carcinoma Cells by Repressing PDGFRB/YAP/CCN2 Axis Activity

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Chinese Journal of Integrative Medicine 2024年第2期30卷 115-124页

作者： LUO Zhi-yi TIAN Qi CHENG Niang-mei LIU Wen-han YANG Ye CHEN Wei ZHANG Xiang-zhi ZHENG Xiao-yuan CHEN Ming-sheng ZHUANG Qiu-yu ZHAO Bi-xing LIU Cong-sheng LIU Xiao-long LI Qin WANG Ying-chao The United Innovation of Mengchao Hepatobiliary Technology key laboratory of fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou(350025)China fujian Pien Tze Huang Enterprise key laboratory of Natural Medicine research and Development Zhangzhou Pien Tze Huang Pharmaceutical Co.Ltd.ZhangzhouFujian Province(363099)China College of Biological Science and Engineering and Mengchao Med-X Center Fuzhou UniversityFuzhou(350116)China Department of Internal Medicine Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou(350025)China

Objective:To investigate the effects of Pien Tze Huang(PZH) on the migration and invasion of HCC cells and underlying molecular ***:Cell counting kit-8(CCK-8) was applied to evaluate the cell viabilities of SMMC-7721,SK-Hep-1,C3A and HL-7702(6 × 10^(3)cells/well) co-incubated with different concentrations of PZH(0,0.2,0.4,0.6,0.8 mg/mL) for 24 ***,wound healing assay,CCK-8and Annexin V-FITC/PI staining were conducted to investigate the effects of PZH on the migration,invasion,proliferation and apoptosis of SK-Hep-1 and SMMC-7721 cells(650 μg/mL for SK-Hep-1 cells and 330 μg/mL for SMMC-7721 cells),*** vivo,lung metastasis mouse model constructed by tail vein injection of HCC cells was used for evaluating the anti-metastasis function of ***-Hep-1 cells(10^(6)cells/200 μL per mice) were injected into B-NDG mice via tail *** 8 mice were randomly divided into PZH and control groups,4 mice in each *** 2-d inoculation,mice in the PZH group were administered with PZH(250 mg/kg,daily) and mice in the control group received only vehicle(PBS) from the 2nd day after xenograft to day *** analysis based on RNA-seq was subsequently used for deciphering anti-tumor mechanism of *** real-time polymerase chain reaction(qRT-PCR) and Western blot were applied to verify RNA-seq *** reporter assay was performed to examine the transcriptional activity of yes-associated protein(YAP).Results:PZH treatment significantly inhibited the migration,invasion,proliferation and promoted the apoptosis of HCC cells in vitro and in vivo(P<0.01).Transcriptome analysis indicated that Hippo signaling pathway was associated with anti-metastasis function of *** study showed PZH significantly inhibited the expressions of platelet derived growth factor receptor beta(PDGFRB),YAP,connective tissue growth factor(CCN2),N-cadherin,vimentin and matrix metallopeptidase 2(MMP2,P<0.01).Meanwhile,the phosphorylatio

关键词： PienTzeHuang hepatocellular carcinoma RNA-seq Hippo yes-associated protein

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CAOSA-extracted lignin improves enzymatic hydrolysis of cellulose

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Green Energy & Environment 2024年第7期9卷 1101-1111页

作者： Sen Ma Zheng Li Jonathan Sperry Xing Tang Yong Sun Lu Lin Jian Liu Xianhai Zeng College of Energy State Key Laboratory of Physical Chemistry of Solid Surfaces Xiamen University fujian Engineering and research Centre of Clean and High-valued Technologies for Biomass Xiamen Key Laboratory of Clean and High-valued Utilization of Biomass Centre for Green Chemical Science University of Auckland

The conversion of biomass into sugar platform compounds is very important for the biorefinery industry. Pretreatment is essential to the biomass of the sugar platform, however, the lignin obtained by pretreatment, as a key part of lignocellulose, generally has a passive effect on the enzymatic hydrolysis of cellulose into sugars. In this study, p-TsOH(p-toluenesulfonic acid), DES(Deep eutectic solvent) and CAOSA(cooking with active oxygen and solid alkali) pretreatment ways were used to fraction lignin from bamboo biomass. After CAOSA treatment, the hydrolysis efficiency of the pulp was 95.57%. Moreover, the effect of different treatment methods on lignin properties was studied and the promotion effect of lignin was investigated by adding it to the cellulose enzymatic hydrolysis system. In this work, the results showed that CAOSA-extracted lignin with lower ?(1.31–1.25) had a better adsorption effect on the enzyme protein. p-TsOH-extracted lignin with a larger S/G ratio enhanced the inhibition of enzymatic hydrolysis. In addition, the presence of-COOHs in lignin could reduce its inhibitory effect on cellulose saccharification.

关键词： Biomass pretreatment CAOSA Cellulose hydrolysis Lignin Enzyme

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Supported Atomically Dispersed Pd Catalyzed Direct Alkoxylation and Allylic Alkylation

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Chinese Journal of Chemistry 2024年第7期42卷 719-724页

作者： Ruixuan Qin Ziwen Chen Qingyuan Wu Nanfeng Zheng Pengxin Liu New Cornerstone Science laboratory State Key Laboratory for Physical Chemistry of Solid SurfacesCollaborative Innovation Center of Chemistry for Energy Materialsand National&Local Joint Engineering Research Center of Preparation Technology of NanomaterialsCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamenFujian 361005China Innovation Laboratry for Scences and Technoogies of Energy Materials of Fujan Province(IKKM) Xiamen Fujian 361102China School of Physical Science and Technology Shanghai Tech UniversityShanghai 201210China fujian key laboratory of Rare-earth Functional Materials Fujian Shanhai Collaborative Innovation Center of Rare-earth Functional MaterialsLongyanFujian 366300China

A new approach to allylic alkylation is realized using an atomically dispersed palladium catalyst(Pd1/TiO2-EG).Unlike conventional methods that require derivation of substrates and utilization of additives,this method allows for direct allylic alkylation from allylic alcohols,producing H2O as the sole *** catalyst's high efficiency is attributed to the local hydrogen bonding at the or-ganic-inorganic interface(Pd-EG interface),facilitating hydroxyl group activation forη3π-allyl complex *** system demonstrates successful direct C—O and C—C coupling reactions with high selectivity,requiring no *** study highlights the potential of supported atomically dispersed catalysts for greener and more efficient catalysis,meanwhile,offers unique insights into the distinct behavior of atomically dispersed catalysts in comparison to homogeneous or nanoparticle-based catalysts.

关键词： Atomically dispersed catalyst Alkylation C—C coupling Organic-inorganic Interface Hydrogen bond Tsuji-Trost reaction Green catalysis

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Restraining growth of Zn dendrites by poly dimethyl diallyl ammonium cations in aqueous electrolytes

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Rare Metals 2024年第8期43卷 3735-3747页

作者： Xiang-Xin Zhang Yuan-Qiang Chen Chang-Xin Lin Yuan-Sheng Lin Guo-Lin Hu Yong-Chuan Liu Xi-Lai Xue Su-Jing Chen Zhan-Lin Yang Bai-Sheng Sa Yi-Ning Zhang key laboratory of Optoelectronic Materials Chemistry and Physics Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Helmholtz Institute Ulm (HIU) Karlsruhe Institute of Technology (KIT) College of Materials Science and Engineering Fuzhou University

Metallic zinc is an excellent anode material for Zn-ion batteries,but the growth of Zn dendrite severely hinders its practical ***,an efficient and economical cationic additive,poly dimethyl diallyl ammonium（PDDA） was reported,used in aqueous Zn-ion batteries electrolyte for stabilizing Zn *** growth of zinc dendrites can be significantly restrained by benefiting from the pronounced electrostatic shielding effect from PDDA on the Zn metal ***,the PDDA is preferentially absorbed on Zn（002） plane,thus preventing unwanted side reactions on Zn *** to the introduction of a certain amount of PDDA additive into the common ZnSO4-based electrolyte,the cycle life of assembled Zn‖Zn cells(1 mA·cm-2and 1 mAh·cm-2) is prolonged to more than 1100 *** response to the perforation issue of Zn electrodes caused by PDDA additives,the problem can be solved by combining foamy copper with zinc *** real application,Zn-ion hybrid supercapacitors and MnO2‖Zn cells were assembled,which exhibited excellent cycling stability with PDDA *** work provides a new solution and perspective to cope with the dendrite growth problem of Zn anode.

关键词： PDDA Electrostatic shielding effect Zn anode Zn dendrites

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Exploring the Cation Regulation Mechanism for Interfacial Water Involved in the Hydrogen Evolution Reaction by In Situ Raman Spectroscopy

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Nano-Micro Letters 2024年第3期16卷 303-312页

作者： Xueqiu You Dongao Zhang Xia‑Guang Zhang Xiangyu Li Jing‑Hua Tian Yao‑Hui Wang Jian‑Feng Li School of Ocean Information Engineering Fujian Provincial Key Laboratory of Oceanic Information Perception and Intelligent ProcessingJimei UniversityXiamen 361021People’s Republic of China State key laboratory of Physical Chemistry of Solid Surfaces MOE Key Laboratory of Spectrochemical Analysis and InstrumentationiChEMCollege of Chemistry and Chemical EngineeringCollege of MaterialsCollege of EnergyXiamen UniversityXiamen 361005People’s Republic of China key laboratory of Green Chemical Media and Reactions Ministry of EducationCollaborative Innovation Center of Henan Province for Green Manufacturing of Fine ChemicalsCollege of Chemistry and Chemical EngineeringHenan Normal UniversityXinxiang 453007People’s Republic of China Innovation laboratory for Sciences and Technologies of Energy Materials of fujian Province(IKKEM) Xiamen 361005People’s Republic of China

Interfacial water molecules are the most important participants in the hydrogen evolution reaction(HER).Hence,understanding the behavior and role that interfacial water plays will ultimately reveal the HER ***,investigating interfacial water is extremely challenging owing to the interference caused by bulk water molecules and complexity of the interfacial ***,the behaviors of interfacial water in different cationic electrolytes on Pd surfaces were investigated by the electrochemistry,in situ core-shell nanostructure enhanced Raman spectroscopy and theoretical simulation *** spectral evidence reveals a red shift in the frequency and a decrease in the intensity of interfacial water as the potential is shifted in the positively *** comparing the different cation electrolyte systems at a given potential,the frequency of the interfacial water peak increases in the specified order:Li+

关键词： In situ Raman Interfacial water Hydrogen evolution reaction Cations

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用于水和二氧化碳电解的高熵纳米结构电催化剂

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Science China Materials 2024年第6期67卷 1791-1803页

作者：王根香陈俊翔温珍海李景虹 CAS key laboratory of Design and Assembly of Functional Nanostructures and Fujian Provincial Key Laboratory of Materials and Techniques toward Hydrogen EnergyFujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China key laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology Tsinghua UniversityBeijing 100084China School of Energy and Power Engineering Jiangsu UniversityZhenjiang 212013China

电催化剂在加速电化学转换中扮演着关键角色,这种转换可以利用日益增长的可再生能源将广泛存在的分子转化为高价值化学品.高熵材料(HEMs),通常为含有至少五种主要元素的固溶体,因其独特的物理化学特性,在电催化领域展现出广阔的应用潜力... 详细信息

电催化剂在加速电化学转换中扮演着关键角色,这种转换可以利用日益增长的可再生能源将广泛存在的分子转化为高价值化学品.高熵材料(HEMs),通常为含有至少五种主要元素的固溶体,因其独特的物理化学特性,在电催化领域展现出广阔的应用潜力.尽管对HEMs的研究兴趣持续增长,但开发适合实际应用的高效HEMs电催化剂及理解其催化机理仍是挑战所在.本论文聚焦分析HEM纳米催化剂在几种关键电化学反应中的前沿应用,首先回顾了常见的HEMs电催化剂制备技术,继而探讨了关于高熵合金在电催化中的理论计算研究,以深化对其电催化应用的理解.此外,文章还概述了HEMs电催化剂在水和二氧化碳电解的关键反应中的应用前景,包括析氢反应、二氧化碳还原反应、析氧反应及其它小分子的氧化反应.通过分析近期的重要成果,本文重点讨论了HEMs电催化剂的结构与电催化性能之间的关系.最后,文章展望了高熵纳米材料在电催化实际应用中面临的关键挑战与未来的机遇.我们期望本论文能够激发更多关于开发和完善HEMs纳米结构电催化剂的研究,探索其可行且可扩展的制备方法,促进其在电催化领域的广泛应用.

关键词：电催化析氢反应二氧化碳还原催化机理电化学反应可再生能源纳米结构析氧反应

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Recent Advances in Mechanistic Understanding of Metal-Free Carbon Thermocatalysis and Electrocatalysis with Model Molecules

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Nano-Micro Letters 2024年第7期16卷 74-97页

作者： Wei Guo Linhui Yu Ling Tang Yan Wan Yangming Lin CAS key laboratory of Design and Assembly of Functional Nanostructures Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002People’s Republic of China Xiamen key laboratory of Rare Earth Photoelectric Functional Materials Xiamen Institute of Rare Earth MaterialsHaixi InstituteChinese Academy of SciencesXiamen 361021People’s Republic of China State key laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002People’s Republic of China Institute of Urban Environment Chinese Academy of SciencesXiamen 361021People’s Republic of China

Metal-free carbon,as the most representative heterogeneous metal-free catalysts,have received considerable interests in electro-and thermo-catalytic reac-tions due to their impressive performance and *** the past decade,well-designed carbon catalysts with tunable structures and heteroatom groups coupled with various characterization techniques have proposed numerous reaction ***,active sites,key intermediate species,precise structure-activity relationships and dynamic evolution processes of carbon catalysts are still rife with controversies due to the monotony and limitation of used experimental *** this Review,we sum-marize the extensive efforts on model catalysts since the 2000s,particularly in the past decade,to overcome the influences of material and structure limitations in metal-free carbon *** both nanomolecule model and bulk model,the real contribution of each alien species,defect and edge configuration to a series of fundamentally important reactions,such as thermocatalytic reactions,electrocatalytic reactions,were systematically *** with in situ techniques,isotope labeling and size control,the detailed reaction mechanisms,the precise 2D structure-activity relationships and the rate-determining steps were revealed at a molecular ***,the outlook of model carbon catalysis has also been proposed in this work.

关键词： Metal-free carbon catalysts Model catalyst Electrocatalysis Active site Reaction mechanisms

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Editorial:special topic on biomaterials and bioinspired materials

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Science China Materials 2024年第8期67卷 2373-2375页

作者： Mingjie Liu Xu Hou Dan Wang key laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191China International research Institute for Multidisciplinary Science Beihang UniversityBeijing 100191China State key laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China research Institute for Biomimetics and Soft Matter Fujian Provincial Key Laboratory for Soft Functional Materials ResearchCollege of Physical Science and TechnologyXiamen UniversityXiamen 361005China State key laboratory of Biochemical Engineering Key Laboratory of Biopharmaceutical Preparation and DeliveryInstitute of Process EngineeringChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Biomaterials have been persistently focused owing to their intricate design and efficient *** order to satisfy the demands of life and medical sciences,large amounts of biomaterials capable of interacting with living systems have been created,thereby achieving the diagnosis,treatment or replacement of cells,tissues,and *** continuous evolution for billions of years,living organisms have eventually developed sophisticated multi-scale structures with diverse biological functions to adapt to the external environment.

关键词： organs diagnosis biomaterials

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