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In situ direct reprogramming of astrocytes to neurons via polypyrimidine tract-binding protein 1 knockdown in a mouse model of ischemic stroke

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Neural Regeneration Research 2024年第10期19卷 2240-2248页

作者： Meng Yuan Yao Tang Tianwen Huang Lining Ke En Huang key Laboratory of Brain Aging and Neurodegenerative Diseases of fujian Province Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Human Anatomy School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Scientific Research Center School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology Fujian Medical University Union HospitalFuzhouFujian ProvinceChina fujian key Laboratory of Vascular Aging Fujian Medical UniversityFuzhouFujian ProvinceChina

In situ direct reprogramming technology can directly convert endogenous glial cells into functional neurons in vivo for central nervous system repair. Polypyrimidine tract-binding protein 1(PTB) knockdown has been shown to reprogram astrocytes to functional neurons in situ. In this study, we used AAV-PHP.e B-GFAP-sh PTB to knockdown PTB in a mouse model of ischemic stroke induced by endothelin-1, and investigated the effects of GFAP-sh PTB-mediated direct reprogramming to neurons. Our results showed that in the mouse model of ischemic stroke, PTB knockdown effectively reprogrammed GFAP-positive cells to neurons in ischemic foci, restored neural tissue structure, reduced inflammatory response, and improved behavioral function. These findings validate the effectiveness of in situ transdifferentiation of astrocytes, and suggest that the approach may be a promising strategy for stroke treatment.

关键词： astrocyte in situ direct reprogramming ischemic stroke miR-30 based shRNA neuron polypyrimidine tract-binding protein 1 transdifferentiation

Active flow control of S-duct by plasma synthetic jet

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2022年第8期23卷 652-668页

作者： Ru-bing LIU Xiao-yin MEI Sheng-hui XUE Yu-wen LU Zhe-zhe SU Qi LIN School of Aerospace Engineering Xiamen UniversityXiamen 361102China fujian key laboratoryof plasmaand magnetic resonanceresearch Xiamen 361102China

Flow separation and secondary flow in the S-duct of an aircraft engine cause severe pressure loss and airflow distortion at the outlet,lowering engine ***,a serial two-electrode plasma synthetic jet(PSJ)actuator array is used to actively control the flow field in the duct and improve its *** results show that the PSJ significantly increases the wall pressure recovery coefficient,suppresses flow separation,and improves the outlet pressure *** primary and secondary orders of the influencing factors are as follows:control position>jet momentum coefficient>excitation frequency>jet *** best jet control position is near the separation location,and the best jet configuration is the‘Λ’*** higher the jet momentum coefficient and excitation frequency,the better the flow *** wall pressure coefficient increases by up to 127.8%,and the outlet steady pressure distortion index decreases by 9.15%.The control mechanism is the direct energy injection into the flow boundary layer through a high-speed jet and the indirect control effect of the induced streamwise *** the one hand,the PSJ suppresses flow separation by improving the ability of the boundary layer to resist the inverse pressure *** the other hand,it reduces pressure distortion by decreasing the intensity of the secondary flow and weakening the *** study thus provides a new technology for the active control of the flow-field characteristics in an S-duct and has significance for guiding the application of synthetic jet technology in S-ducts.

关键词： S-duct Flow control Plasma synthetic jet(PSJ) Flow separation Pressure distortion

Clinical and genetic heterogeneity of adult polyglucosan body disease caused by GBE1 biallelic mutations in China

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Genes & Diseases 2024年第5期11卷 17-20页

作者： Yikun Chen Yan Shi Yuan Gao Yan Hu Linying Zhou Jingmei Hong Shirui Gan Xiang Lin Wanjin Chen Guorong Xu Jin He Department of Neurology and Institute of Neurology of First Affiliated Hospital Institute of Neuroscienceand Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouFujian 350005China fujian key Laboratory of Molecular Neurology Institute of NeuroscienceFujian Medical UniversityFuzhouFujian 350005China Electron Microscopy Lab of Public Technology Service Center Fujian Medical UniversityFuzhouFujian 350005China

Adult polyglucosan body disease(APBD)is a rare and highly heterogeneous glycogen storage disorder due to biallelic variants in GBE1.1 Typical APBD presentations include gait abnormalities with polyneuropathy,leukodystrophy,neurogenic bladder,and mild cognitive *** diagnosis of APBD encompasses a large spectrum of conditions including axonal and demyelinating sensorimotor polyneuropathy,progressive spastic paraparesis,and leukodystrophies.

关键词： GBE bladder diagnosis

The OsBSK1-2-MAPK module regulates blast resistance in rice

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The Crop Journal 2024年第1期12卷 110-120页

作者： Shengping Li Xinquan Xiang Zhijuan Diao Na Xia Ling Lu Jing Zhang Zhiwei Chen Dingzhong Tang State key Laboratory of Ecological Control of fujian-Taiwan Crop Pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina College of Life Science Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina fujian Provincial key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina

Receptor-like cytoplasmic kinase OsBSK1-2 was reported to play an important role in regulation of response to rice blast,but the signaling pathway remained *** this study,we identified OsMAPKKK18 and previously uncharacterized MAPKKKs OsMAPKKK16 and OsMAPKKK19 that interact with *** of all three MAPKKKs was induced by Magnaporthe oryzae infection,and all three induced cell death when transiently expressed in Nicotiana benthamiana *** of OsMAPKKK16 and OsMAPKKK18 compromised blast resistance and overexpression of OsMAPKKK19 increased blast resistance,indicating that all three MAPKKKs are involved in regulation of rice blast ***,both OsMAPKKK16 and OsMAPKKK19 interacted with and phosphorylated OsMKK4 and OsMKK5,and chitin-induced MAPK activation was suppressed in osmapkkk16 and osbsk1-2 ***18 was earlier reported to interact with and phosphorylate OsMKK4 and affect chitin-induced MAPK activation,suggesting that OsBSK1-2 is involved in regulation of immunity through multiple MAPK signaling *** BSK1 in Arabidopsis,OsBSK1-2 was not involved in response to avirulent *** *** together,our results revealed important roles of OsMAPKKK16/18/19 and a OsBSK1-2-OsMAPKKK16/18/19-OsMKK4/5 module in regulating response to rice blast.

关键词： Disease resistance Magnaporthe oryzae Oriza sativa Rice blast

Quantum nonlocality evolution for two entangled mesoscopic fields under decoherence

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Communications in Theoretical Physics 2024年第1期76卷 24-28页

作者： Zhao-Song Tan Shou-Bang Yang Zhen-Biao Yang Shi-Biao Zheng fujian key Laboratory of Quantum Information and Quantum Optics Fuzhou UniversityFuzhouFujian 350116China

Investigation of the nonlocality evolution of entangled mesoscopic fields under decoherence not only is important for understanding the quantum–classical transition,but also has relevance to quantum communication and quantum computation protocols based on continuous *** to previous formulations of Bell inequalities,the system loses nonlocal features far before the disappearance of *** here construct a new version of Bell signal based on rotated and displaced on–off correlations,with which the Bell inequality is violated as long as there remains entanglement and the field state components keep ***,the nonlocal character revealed by our formulation decays much slower compared with those based on previous *** importantly,there exists a wide regime where the Bell inequality is restored with previous formulations but remains to be violated based on our correlation operators.

关键词： entangled mesoscopic fields rotated and displaced on-off correlations Bell inequality nonlocality evolution

Transcription factor OsSPL10 interacts with OsJAmyb to regulate blast resistance in rice

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The Crop Journal 2024年第1期12卷 301-307页

作者： Zaofa Zhong Lijing Zhong Xiang Zhu Yimin Jiang Yihong Zheng Tao Lan Haitao Cui College of Life Science Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina fujian Provincial key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina College of Plant Protection Shandong Agricultural UniversityTai’an 271018ShandongChina

Transcription factors(TFs)play essential roles in transcriptional reprogramming during activation of plant immune responses to ***10(SQUAMOSA promoter binding protein-like10)is an important TF regulating trichome development and salt tolerance in *** we report that knockout of OsSPL10 reduces whereas its overexpression enhances rice resistance to blast ***10 positively regulates chitin-induced immune responses including reactive oxygen species(ROS)burst and callose *** show that OsSPL10 physically associates with OsJAmyb,an important TF involved in jasmonic acid(JA)signaling,and positively regulates its protein *** then prove that OsJAmyb positively regulates resistance to *** results reveal a molecular module consisting of OsSPL10 and OsJAmyb that positively regulates blast resistance.

关键词： Immunity Jasmonate Oryza sativa OsSPL10 Transcription factor

Programmable and reconfigurable humidity-driven actuators made with MXene(Ti_(3)C_(2)T_(x))-cellulose nanofiber composites for biomimetic applications

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Nano Research 2024年第7期17卷 6619-6629页

作者： Shaofeng Zeng Yuanji Ye Peidi Zhou Shimin Yi Qiaohang Guo Huamin Chen Guozhen Shen Mingcen Weng School of Mechanical and Automotive Engineering Fujian University of TechnologyFuzhou 350118China School of Materials Science and Engineering Fujian Provincial Key Laboratory of Advanced Materials Processing and ApplicationKey Laboratory of Polymer Materials and Products of Universities in FujianFujian University of TechnologyFuzhou 350118China Institute of Smart Marine and Engineering Fujian University of TechnologyFuzhou 350118China fujian key Laboratory of Functional Marine Sensing Materials College of Materials and Chemical EngineeringMinjiang UniversityFuzhou 350108China School of Integrated Circuits and Electronics Beijing Institute of TechnologyBeijing 100081China

Smart actuators have a wide range of applications in bionics and energy *** ability to reconfigure shape is essential for soft actuators to achieve various shapes and deformations,which is a crucial feature for next-generation ***,it is still an enormous challenge to establish a straightforward approach to creating programmable and reconfigurable ***-cellulose nanofiber composite film(MCCF)with a brick-and-mortar hierarchical structure was produced through a vacuum filtration *** demonstrates impressive mechanical properties such as a tensile stress of 68 MPa and a Young’s modulus of 4.65 ***,the MCCF highlights its potential for water-assisted shaping/welding due to the abundance of hydrogen bonds between MXene and cellulose *** also showcases capabilities as a humiditydriven actuator with a rapid response rate of 550°·s^(−1).Using the methods of water-assisted shaping/welding,several bionic actuators(such as flower,butterfly,and muscle)based on MCCF were designed,highlighting their versatility in applications of smart *** research showcases the impressive capabilities of MXene-based actuators and offers beneficial insights for the advancement of future intelligent materials.

关键词： MXene cellulose nanofibers programmable actuators humidity-driven

Activation of the wnt/β-catenin/CYP1B1 pathway alleviates oxidative stress and protects the blood-brain barrier under cerebral ischemia/reperfusion conditions

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Neural Regeneration Research 2024年第7期19卷 1541-1547页

作者： Xingyong Chen Nannan Yao Yanguang Mao Dongyun Xiao Yiyi Huang Xu Zhang Yinzhou Wang Department of Neurology Fujian Provincial HospitalShengli Clinical Medical College of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology Cangzhou Central HospitalCangzhouHebei ProvinceChina fujian Academy of Medical Science FuzhouFujian ProvinceChina key Testing Laboratory of fujian Province FuzhouFujian ProvinceChina

Accumulating evidence suggests that oxidative stress and the Wnt/β-catenin pathway participate in stroke-induced disruption of the blood-brain ***,the potential links between them following ischemic stroke remain largely *** present study found that cerebral ischemia leads to oxidative stress and repression of the Wnt/β-catenin ***,Wnt/β-catenin pathway activation by the pharmacological inhibito r,TWS119,relieved oxidative stress,increased the levels of cytochrome P4501B1(CYP1B1)and tight junction-associated proteins(zonula occludens-1[ZO-1],occludin and claudin-5),as well as brain microvascular density in cerebral ischemia *** r,rat brain microvascular endothelial cells that underwent oxygen glucose deprivation/reoxygenation displayed intense oxidative stress,suppression of the Wnt/β-catenin pathway,aggravated cell apoptosis,downregulated CYP1B1and tight junction protein levels,and inhibited cell prolife ration and *** ofβ-catenin or knockdown ofβ-catenin and CYP1B1 genes in rat brain mic rovascular endothelial cells at least partly ameliorated or exacerbated these effects,*** addition,small interfering RNA-mediatedβ-catenin silencing decreased CYP1B1 expression,whereas CYP1B1 knoc kdown did not change the levels of glycogen synthase kinase 3β,Wnt-3a,andβ-catenin proteins in rat brain microvascular endothelial cells after oxygen glucose deprivatio n/***,the data suggest that CYP1B1 can be regulated by Wnt/β-catenin signaling,and activation of the Wnt/β-catenin/CYP1B1 pathway contributes to alleviation of oxidative stress,increased tight junction levels,and protection of the blood-brain barrier against ischemia/hypoxia-induced injury.

关键词： blood-brain barrier CYP1B1 oxidative stress oxygen glucose deprivation/reoxygenation tight junction vascular endothelial cells Wnt/β-catenin pathway β-catenin

Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期12卷 549-557页

作者： Yan Zheng Ei Ei Khine Khin Mar Thi Ei Ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina fujian Provincial key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation