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Manila clam Venerupis philippinarum as a biomonitor to metal pollution

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Chinese Journal of Oceanology and Limnology 2013年第1期31卷 65-74页

作者：吴惠丰吉成龙王清刘小莉赵建民冯江华 key laboratory of Coastal Zone Environmental Processes Yantai Institute of Coastal Zone Research (YIC)Chinese Academy of Sciences (CAS) Shandong Provincial key laboratory of Coastal Zone Environmental Processes YICCAS Graduate University of Chinese Academy of Sciences Department of Electronic Science Fujian Key Laboratory of Plasma and Magnetic ResonanceState Key Laboratory of Physical Chemistry of Solid SurfacesXiamen University

The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate significant biological differences in gills of white and zebra clams based on metabolic profiles and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profiles, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

关键词： Manila clam Venerupisphilippinarum biomonitor biomarker metabolomics

Inhibitory Kinetics of p-Substituted Benzaldehydes on Polyphenol Oxidase from the Fifth Instar of Pieris Rapae L.

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Tsinghua Science and Technology 2007年第4期12卷 400-404页

作者：王勤柯莉娜薛超彬罗万春陈清西 key laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University key laboratory of Pesticide toxicology and Application Technique College of Plant Protection Shandong Agriculture University

Polyphenol oxidase （PPO） is the enzyme responsible for enzymatic browning during the growth of insects. It is also involved in defense reactions and is related with immunities in insects. PPO a metalloenzyme oxidase, catalyzes the oxidation of o-diphenol to o-quinone. The present paper describes the effects of benzaldehyde and its p-substituted derivatives on the activity of PPO from the fifth instar of Pieris rapae L. PPO from the fifth instar of Pieris rapae L. was purified using ammonium sulfate fractionation and chromatography on Sephadex G-100. The enzyme kinetics was characterized using L-3,4-dihydroxyphenylalanine （L-DOPA） as substrate. The results show that benzaldehyde, phydroxybenzaldehyde, p-chlorobenzaldehyde, and p-cyanobenzaldehyde can inhibit the PPO activity for the oxidation of L-DOPA. The inhibitor concentration leading to 50% activity lost, IC50, was estimated to be 5.90, 5.62, 2.83, and 2.91 mmol/L for the four tested inhibitors, respectively. Kinetic analyses show that the inhibitory effects of these compounds are reversible. Benzaldehyde, phydroxybenzaldehyde, and p-chlorobenzaldehyde are noncompetitive inhibitors while p-cyanobenzaldehyde is a mixed-type inhibitor. The inhibition constants were determined for all four inhibitors. p-chlorobenzaldehyde and p-cyanobenzaldehyde were more potent inhibitors than the other compounds. These results provide a basis for developing PPO inhibition-based pesticides.

关键词： Pieris rapae L. the fifth instar polyphenol oxidase benzaldehyde p-substituted derivatives inhibition inhibitory type kinetics mechanism

Facilitation of behavioral and cortical emergence from isoflurane anesthesia by GABAergic neurons in basal forebrain

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中国药理学与毒理学杂志 2023年第7期37卷 485-486页

作者： CAI Ping SU Weikun ZHANG Jinsheng LIU Peichang LIU Feng LIU Renfu LI Zhangshu ZHU Zhonghua XIAO Wenhao HU Yonghuai CAI Hongda WU Xiaodan ZHANG Liangcheng YU Changxi CHEN Li Department of Pharmacology School of PharmacyFujian Medical University fujian Province key laboratory of Environment and Health School of Public HealthFujian Medical University School of Basic Medical Sciences Fujian Medical University Department of Anesthesiology Fujian Medical University Union Hospital Department of Anesthesiology Shengli Clinical Medical College of Fujian Medical UniversityFujian Provincial Hospital Department of Anesthesiology First Affiliated Hospital of Fujian Medical UniversityFujian Medical University fujian key laboratory of Drug Target Discovery and Structural and Functional Research

OBJECTIVE To reveal the role of the basal forebrain (BF) GABAergic neurons in the regulation of isoflurane anesthesia and to elucidate the underlying neural pathways. METHODS The activity of BF GABAergic neurons was monitored during isoflurane anesthesia using a genetically encoded calcium indicator in VgatCre mice of both sexes. The activity of BF GABAergic neurons was manipulated by chemogenetic and optogenetic approaches. Sensitivity, induction time and emergence time of isoflurane anesthesia were estimated by righting reflex. The electroencephalogram (EEG) power and burst-suppression were monitored by EEG *** effects of activation of GABAergic BF-thalamic reticular nucleus (TRN) pathway on isoflurane anesthesia were investigated with optogenetics. RESULTS The activity of BF GABAergic neurons was generally inhibited during isoflurane anesthesia, obviously decreased during the induction of anesthesia and gradually restored during the emergence from anesthesia. Activation of BF GABAergic neurons with chemogenetics and optogenetics promoted behavioral emergence from isoflurane anesthesia, with decreased sensitivity to isoflurane, delayed induction and accelerated emergence from isoflurane *** activation of BF GABAergic neurons promoted cortical activity during isoflurane anesthesia, with decreased EEG delta power and burst suppression ratio during 0.8%and 1.4%isoflurane anesthesia, *** to the effects of activating BF GABAergic cell bodies, photostimulation of BF GABAergic terminals in the TRN also strongly promoted cortical activation and behavioral emergence from isoflurane anesthesia. CONCLUSION The GABAergic neurons in the BF is a key neural substrate for general anesthesia regulation that facilitates behavioral and cortical emergence from general anesthesia via the BF-TRN pathway.

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Effectiveness of an ocular adhesive polyhedral oligomeric silsesquioxane hybrid thermo-responsive FK506 hydrogel in a murine model of dry eye

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Bioactive Materials 2022年第3期7卷 77-91页

作者： Yi Han Lu Jiang Huihui Shi Chenfang Xu Minting Liu Qingjian Li Lan Zheng Hong Chi Mingyue Wang Zuguo Liu Mingliang You Xian Jun Loh Yun-Long Wu Zibiao Li Cheng Li fujian Provincial key laboratory of Ophthalmology and Visual Science&Ocular Surface and Corneal Diseases Eye Institute&Affiliated Xiamen Eye CenterSchool of MedicineXiamen UniversityXiamen361102China Institute of Materials Research and Engineering A*STAR(Agency for ScienceTechnology and Research)Singapore138634Singapore School of Chemical Sciences University of Chinese Academy of ScienceBeijing100049China key laboratory of Marine Materials and Related Technologies Zhejiang Key Laboratory of Marine Materials and Protective TechnologiesNingbo Institute of Material Technology and EngineeringChinese Academy of ScienceNingbo315201China fujian Provincial key laboratory of Innovative Drug Target Research and State key laboratory of Cellular Stress Biology School of Pharmaceutical SciencesXiamen UniversityXiamen361102China Hangzhou Cancer Institute Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang ProvinceAffiliated Hangzhou Cancer HospitalZhejiang University School of MedicineHangzhou310002China Shandong Provincial key laboratory of Molecular Engineering School of Chemistry and Pharmaceutical EngineeringQilu University of Technology(Shandong Academy of Sciences)Jinan250353China Department of Materials Science and Engineering National University of SingaporeSingapore117575Singapore

Dry eye is a common ocular disease that results in discomfort and impaired vision,impacting an individual’s quality of life.A great number of drugs administered in eye drops to treat dry eye are poorly soluble in water and are rapidly eliminated from the ocular surface,which limits their therapeutic ***,it is imperative to design a novel drug delivery system that not only improves the water solubility of the drug but also prolongs its retention time on the ocular ***,we develop a copolymer from mono-functional POSS,PEG,and PPG(MPOSS-PEG-PPG,MPEP)that exhibits temperature-sensitive sol-gel transition *** thermo-responsive hydrogel improves the water solubility of FK506 and simultaneously provides a mucoadhesive,long-acting ocular delivery *** addition,the FK506-loaded POSS hydrogel possesses good biocompatibility and significantly improves adhesion to the ocular *** comparison with other FK506 formulations and the PEG-PPG-FK506(F127-FK506)hydrogel,this novel MPOSS-PEG-PPG-FK506(MPEP-FK506)hydrogel is a more effective treatment of dry eye in the murine dry eye ***,delivery of FK506 in this POSS hydrogel has the potential to prolong drug retention time on the ocular surface,which will improve its therapeutic efficacy in the management of dry eye.

关键词： Ocular adhesive Polyhedral oligomeric silsesquioxane FK506 Dry eye

ADMETNet：The knowledge base of pharmacokinetics and toxicology network

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Journal of Genetics and Genomics 2017年第5期44卷 273-276页

作者： Quan Xu Ke Liu Xingming Lin Yangmei Qin Linshan Chen Jiao Cheng Mindong Zhong Qiushun He Yinbo Li Tingwu Wang Jianbo Pan Menglu Peng Lixia Yao Zhiliang Ji State key laboratory of Stress Cell Biology School of Life SciencesXiamen UniversityXiamen 361102China Department of Health Sciences Research Mayo ClinicRochesterMN 55905USA The key laboratory 0r Chemical Biology of Fujian ProvinceXiamen UniversityXiamen 361005China

A good drug or drug candidate should not only interact with its target molecule effectively and specifically,but also be absorbed into the body,distributed to the right location,metabolized into right compounds,and eliminated out of the body in proper *** processes of drug getting into and out of the body involve Absorption,Distribution,Metabolism,and Excretion（ADME）,

关键词： interact eliminated getting Absorption absorbed manner candidate toxicity Browse Pathway

A novel nanoparticle drug delivery system:tumor-exosome encapsulated DOX for breast cancer treatment and cancer metastasis chemoprevention

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中国药理学与毒理学杂志 2019年第10期 895-896页

作者： ZHOU Yu XIE Xiao-dong NIE Hui-fang JIA Lee Cancer Metastasis Alert and Prevention Center and Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment College of ChemistryFujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy Fuzhou University Institute of Oceanography Minjiang University

OBJECTIVE To demonstrate the advantages of tumor exosomes as carriers for "natural nanoparticles" for drug delivery. METHODS The differences in various aspects of exosomes(such as particle size,potential, surface protein, etc.) before and after drug loading(DOX) were compared. Flow cytometry analysis and distribution of exosomes in mice verified the specificity of tumor exosomes for distal tissues, and additionally analyzed the natural targeting of tumor exosomes by confocal experiments and flow cytometry. Tumor exosomes surface protein knockout experiments demonstrate that exosomes indicate that proteins can increase drug delivery efficiency. Animal experiments verify that tumor exosomes loaded with drugs(DOX) inhibit tumor growth and tumor metastasis. RESULTS There were no significant differences in various aspects of exosomes(such as particle size, potential, surface protein, etc.) before and after drug loading(DOX). Confocal and flow analysis showed that the tumor exosomes had good targeting. CD47 knockout experiments demonstrate that tumor exosomes act as drug carriers to reduce the body′ s immune response and enhance drug targeting and delivery efficiency. CONCLUSION Tumor exosomes have great potential and advantages as a targeted drug carrier in the treatment of tumors and prevention of tumor metastasis.

关键词： exosome tumor metastasis drug deliver

Revealing unconventional host-guest complexation at nanostructured interface by surface-enhanced Raman spectroscopy

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Light(Science & Applications) 2021年第5期10卷 898-905页

作者： Gan-Yu Chen Yi-Bin Sun Pei-Chen Shi Tao Liu Zhi-Hao Li Si-Heng Luo Xin-Chang Wang Xiao-Yu Cao Bin Ren Guo-Kun Liu Liu-Lin Yang Zhong-Qun Tian State key laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials(iChEM)College of Chemistry and Chemical EngineeringXiamen UniversityXiamen361005China State key laboratory of Marine Environmental Science Fujian Provincial Key Laboratory for Coastal Ecology and Environmental StudiesCenter for Marine Environmental Chemistry&ToxicologyCollege of the Environment and EcologyXiamen UniversityXiamen361102China School of Electronic Science and Engineering(National Model Microelectronics College) Xiamen UniversityXiamen361005China key laboratory of Chemical Biology of fujian Province Xiamen UniversityXiamen361005China

Interfacial host–guest complexation offers a versatile way to functionalize ***,the complicated interfacial environment and trace amounts of components present at the interface make the study of interfacial complexation very ***,taking the advantages of near-single-molecule level sensitivity and molecular fingerprint of surface-enhanced Raman spectroscopy(SERS),we reveal that a cooperative effect between cucurbit[7]uril(CB[7])and methyl viologen(MV^(2+)2^(I−))in aggregating Au NPs originates from the cooperative adsorption of halide counter anions I^(−),MV^(2+),and CB[7]on Au NPs ***,similar SERS peak shifts in the control experiments using CB[n]s but with smaller cavity sizes suggested the occurrence of the same guest complexations among CB[5],CB[6],and CB[7]with MV2+.Hence,an unconventional exclusive complexation model is proposed between CB[7]and MV^(2+)on the surface of Au NPs,distinct from the well-known 1:1 inclusion complexation model in aqueous *** summary,new insights into the fundamental understanding of host–guest interactions at nanostructured interfaces were obtained by SERS,which might be useful for applications related to host–guest chemistry in engineered nanomaterials.

关键词： materials. surface. complexation

Ameliorating Effect and Potential Mechanism of Camellia Oil on Constipated Mice

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Journal of Beijing Institute of Technology 2018年第2期27卷 312-318页

作者： Liangshun Cao Tianyu Liu Chunhui Cai Yan Li Chongming Wu College of Life Science Beijing Institute of Technology fujian Chunhui Bioengineering Co . Ltd. Pharmacology and toxicology Research Center Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences & Peking Union Medical College

Constipation is a global epidemic. To evaluate the ameliorating effect of camellia oil on constipation,two slowtransit costive mice models were established by water deprivation or sucralfate gavage. Administration of camellia oil（ 4. 0 and 8. 0 mL/kg/day） significantly shortened the defecation time,increased defecation mass and fecal water content,with efficacies comparable to that of hemp seed oil. Intestinal propulsion test showed that camellia oil significantly enhanced the propelling rates of the charcoal powder in the intestine. Meanwhile,camellia oil also significantly decreased the serum levels of nitric oxide（ NO）,nitric oxide synthase（ NOS） and vasoactive intestinal peptide（ VIP） and increased serum substance P in costive animals. These results suggested that camellia oil can largely relieve slowtransit constipation and enhance the gastrointestinal motility through modulation of serum gastrointestinal motility key factors such as NO,NOS and VIP and substance P.

关键词： camellia oil constipation nitric oxide(NO) nitric oxide synthase(NOS) vasoactive intestinal peptide(VIP) substance P

Bioinformatics Analysis and Identification of key Candidate Genes Associated with Nephrotoxicity Induced by Cobalt

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Biomedical and Environmental Sciences 2023年第2期36卷 201-205页

作者： WANG Rui ZHANG Ding ZHUGE Rui Jian XUE Qian HE Chang Hao MA Wen Xuan SHEN Zhu Bin GUO Li Department of toxicology School of Public HealthJilin UniversityChangchun 130021JilinChina Department of Spine Surgery China-Japan Union HospitalJilin UniversityChangchun 130033JilinChina Department of Security Xiamen Rehabilitation HospitalXiamen 36100FujianChina

Cobalt(Co)is a silver-gray,high-intensity,widely distributed metal element that exists in cobalt compounds,and its common valences are bivalence(Co2+)and trivalence(Co3+)[1].The main routes of Co-exposure are occupational and environmental *** human body can be exposed to high concentrations of Co2+through inhalation of contaminated air,consumption of contaminated food and water,or ingestion of Co-containing supplements[2].

关键词： valence silver cobalt