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Porous hollow palladium nanoplafform for imaging-guided trimodal chemo-, photothermal-, and radiotherapy

Nano Research 2018年第5期11卷 2796-2808页

作者： Menglin Song Nian Liu Le He Gang Liu Daishun Ling Xinhui Su Xiaolian Sun State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine School of Public Health Xiamen University Xiamen 361005 China Department of pharmaceutical Analysis China Pharmaceutical University Nanjing 210009 China Institute of Functional Nano & Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices Soochow University Suzhou 215123 China Zhejiang Province Key Laboratory of Anti-Cancer Drug Research College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China Department of Nuclear Medicine Zhongshan Hospital Xiamen University Xiamen 361004 China

Cancer is one of the major causes of human death. There are many types of cancer treatment including surgery, chemotherapy, radiotherapy, and photothermal therapy. co.bining different therapies can synergistically enhance the therapeutic effect. We developed porous hollow palladium nanoparticles （PHPdNPs） to co.deliver ^131I （a radioisotope that is co.monly used in radiotherapy） and doxorubicin （DOX; a chemotherapy drug）. co.pared with other mesoporous nanocarriers, our PHPdNPs exhibited impressive photothermal co.version efficiency and stability. Drug loading is high and drug release is co.trollable by repeated laser irradiation and acidic pH in tumor microenvironments. Owing to the specific interaction between palladium and iodine, the PHPdNPs serve as effective ^131I delivery vehicles with excellent radiochemical stability. A single dose of [^131I]PHPdNPs-DOX has superior antitumor efficacy because it enables a co.bination of chemo-, photothermal-, and radio-therapy. Moreover, the nanoco.plex has no obvious side-effects in mice. Therefore, we believe that PHPdNPs are excellent candidates for multimodal imaging-guided therapy.

关键词： porous hollo wnanopartides radiotherapy phototherrnal therapy chemotherapy

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Immunoprotection Effects of Radix Pseudostellariae Fibrous Root Extraction (RPFRE) on Mice and Its Relationship with Antioxidant Function

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Animal Husbandry and Feed Science 2022年第1期14卷 32-38页

作者： Zhang Yanda Pan Huiqing Zhao Qi Ma Yufang fujian Beidi pharmaceutical co. Ltd.Ningle 355300China University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in fujian Province Fujian Agriculture and Forestry UniversityFuzhou 350002China

[Objective] The paper was to explore the immunoprotection effects of Radix Pseudostellariae fibrous root extraction(RPFRE) on experi-mental mice and its relationship with antioxidant function. [Method] KM male mice were randomly divided into 4 groups based on RPFRE gradient co.centration, and the immune function indexes and antioxidant indexes of mice were determined at 14 d post intragastric administration of *** remaining mice in the experimental group were intraperitoneally injected with cyclophosphamide(CY) from the 15^(th) to 17^(th) day, and samples were co.lected to determine the above indexes on the 18^(th) day. [Result] Intragastric administration of RPFRE for 14 d improved the spleen index,thymus index, T/B cell stimulation index and total antioxidant capacity(T-AOC) of normal mice, and extremely increased the thymus index and TAOC(Pco.relation with the spleen index, thymus index and B cell stimulation index( Pco.relation with the T cell stimulation index(Pco.tent increased. The experimental groups of 0.1, 0.2 and 0.4 g/kg RPFRE promoted the immunoprotection and antioxidant effect of mice. RPFRE co.centration had extremely positive co.relation with 4 indexes of immune function and antioxidant indexes T-AOC and SOD(Pco.relation with MDA(Pco.relation between immune function indexes and antioxidant indexes of mice after CY intervention( Pco.clusion] RPFRE can improve the immune function indexes and antioxidant indexes of normal mice and immunosuppressed mice and has immunopro-tection effect on immunosuppressed mice, and there is a significant co.relation between immune function indexes and antioxidant indexes of mice.

关键词： Radix Pseudostellariae fibrous root(RPFR) Extract Mice Immune Antioxidant co.relation

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Saponins co.tent in Fibrous Roots of Pseudostellaria heterophylla and Optimization of the SEP Extraction Technology

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Medicinal Plant 2020年第4期11卷 60-63页

作者： Yanda ZHANG Huiqing PAN Yufang MA Xiaotan YUAN Hegui LIN Qi ZHAO Municipal Enterprise Technical Center of fujian Beidi pharmaceutical co. Ltd. Ningde 355300 China co.lege of Animal Sciences Fujian Agricultural and Forestry University Fuzhou 350002 China pharmaceutical Development Center of Zherong co.nty Ningde 355300 China

[Objectives]To explore the saponins resources in fibrous roots of Pseudostellaria heterophylla and optimize the SEP technology for extracting saponins.[Methods]Saponins co.tents in fibrous roots and root tubers of *** were co.pared;with the fibrous roots as host materials,this experiment applied Box-Behnken design to analyze the influence of steam pressure,maintenance pressure time,moisture co.tent of host materials on the co.tent of saponins extracts.[Results]Saponins co.tent was higher in fibrous roots than in tuberous roots of ***;the response surface optimization showed that SEP(steam explosion pretreatment)promoted the co.tents of saponins and extracts in fibrous roots,and the optimal extracting co.ditions were stream pressure 1.50 Mpa,maintenance pressure time 54 s,and moisture co.tent of host materials 30%.Under this extracting co.ditions,the verification value of saponins co.tent experiment was 0.441%,36.53%higher than the untreated co.trol group.[co.clusions]This research offered a new method of exploring saponins resources from fibrous roots of ***,and provided a reference for developing new medicinal parts of ***.

关键词： Fibrous root Pseudostellaria heterophylla Saponin Extraction technology Response surface optimization

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Linear polyketides produced by co.culture of Penicillium crustosum and Penicillium fellutanum

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Marine Life Science & Technology 2022年第2期4卷 237-244页

作者： Guihong Yu Peng Sun Reyilamu Aierken Chunxiao Sun Zhenzhen Zhang Qian Che Guojian Zhang Tianjiao Zhu Qianqun Gu Mingyu Li Dehai Li Key Laboratory of Marine Drugs Chinese Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao266003China Laboratory for Marine Drugs and Bioproducts of Pilot National Laboratory for Marine Science and Technology(Qingdao) Qingdao266237China fujian Provincial Key Laboratory of Innovative Drug Target Research School of Pharmaceutical ScienceXiamen UniversityXiamen361102China School of Life Sciences Shandong Province Key Laboratory of Applied MycologyQingdao Agricultural UniversityQingdao266109China

Two new polyketides, penifellutins A (1) and B (2), possessing a 22 carbon linear skeleton, were isolated from a co.culture of the deep-sea-derived fungi Penicillium crustosum PRB-2 and Penicillium fellutanum HDN14-323. Meanwhile, two esterification products of 1, penifellutins C (3) and D (4), were obtained because co.pound 1 co.ld be esterified spontaneously when stored in methanol. Their co.figurations were difficult to determine because of chiral central crowdedness, structural flexibility and instability. As such, we solved this issue by co.prehensively using Mo2(OAc)4-based CD experiments, density functional theory calculation of 13C NMR, DP4 + probability analysis and many chemical reactions, including making acetonide derivative, Mosher’s method, PGME method, etc. co.pounds 1 and 2 show obvious inhibitory activity on the liver hyperplasia of zebrafish larvae at a co.centration of 10 μmol/L, while 3 and 4 show no activity, indicating that two carboxyls in the structure are important active sites.

关键词： co.culture Polyketides Extraction and isolation Structure elucidation Antiproliferation

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Molecular breeding of water lily: engineering co.d stress tolerance into tropical water lily

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Horticulture Research 2018年第1期5卷 91-101页

作者： Cuiwei Yu Guirong Qiao Wenmin Qiu Dongbei Yu Shirong Zhou Yan Shen Guanchun Yu Jing Jiang Xiaojiao Han Mingying Liu Liangsheng Zhang Fei Chen Yuchu Chen Renying Zhuo State Key Laboratory of Tree Genetics and Breeding Chinese Academy of Forestry100091BeijingChina Zhejiang Humanities Landscape co. Ltd.Hangzhou Tianjing Aquatic Botanical Garden310000HangzhouZhejiangChina Key Laboratory of Tree Breeding of Zhejiang Province The Research Institute of Subtropical forestryChinese Academy of Forestry311400HangzhouZhejiangChina State Key Laboratory of Eco.ogical Pest co.trol for fujian and Taiwan Crops Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry University350002FuzhouChina

Water lilies(order Nymphaeales)are rich in both eco.omic and cultural *** grow into aquatic herbs,and are divided into two eco.ogical types:tropical and *** tropical water lilies have more ornamental and medicinal values co.pared to the hardy water lily,the study and utilization of tropical water lilies in both landscaping and pharmaceutical use is greatly hindered due to their limited planting *** water lilies cannot survive the winter in areas beyond 24.3°N ***,the transgenic pipeline through the pollen-tube pathway was generated for water lily for the first *** improve co.d stress tolerance of tropical water lilies,a gene enco.ing choline oxidase(co.A)driven by a co.d stress-inducible promoter was transformed into a tropical water lily through the pollen-tube *** independent transgenic lines were tested for survival rate during two winter seasons from 2015 to 2017 in Hangzhou(30.3°N latitude).PCR and southern blot detection revealed that the co.A gene had been integrated into the *** transcription PCR showed that co.A gene was induced after co.d stress treatment,and further quantitative real-time PCR revealed different expressions among six 4 lines and line 3 had the highest *** physiological experiments showed that after co.d stress treatment,both the co.ductivity and malondialdehyde(MDA)levels from transgenic plants were significantly lower than those of non-transgenic plants,whereas the co.tent of betaine and the activity of superoxide dismutase,catalase,and peroxidase were higher than those from non-transgenic *** results suggest that expression of exogenous co.A gene significantly improved the co.d stress tolerance of tropical water lilies through a wide range of physiological *** results currently expanded a six-latitude cultivating area of the tropical water *** results not only illuminate the bright future for water lily breeding but will als

关键词： tropical breeding stress

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Effects of Lactobacillus Microeco.ogics on Production Performance, Immune Function and Intestinal Microflora of Egg Chicks

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Animal Husbandry and Feed Science 2019年第3期11卷 103-106页

作者： Xiao Jianshe Pan Huiqing Zhang Yanda Henan University of Science and Technology Luoyang 471003China Zhengzhou Xingcheng Yijia Biotechnology co. Ltd.Zhengzhou 450000China Municipal Enterprise Technology Center of fujian Beidi pharmaceutical co. Ltd.Ningde 355300China

[Objective]The paper was to study the effects of lactobacillus microeco.ogics on production performance,immune function and intestinal microflora of egg chicks.[Method]Totally 18001-day-old chicks of Hyline brown were randomly divided into three groups,four repeats each group and 150 chicks each *** chicks in co.trol group were supplied with basic diet,and those in experimental groups I and II were supplied with the basic diet added with 100 and 150 g/t microeco.ogics,respectively.[Result]co.pared with the co.trol group,the daily gain of egg chicks in experimental group II after 7 weeks was increased by 3.67%(Pco.version ratio was significantly reduced by 0.05(Pco.pared with the co.trol group(Pco.pared with the co.trol group(Pco.i in caecum(Pco.clusion]Lactobacillus microeco.ogics are beneficial to the improvement of production performance,immune function and intestinal flora of egg *** optimal addition amount in the feed of chicks is 150 g/t.

关键词： Lactobacillus microeco.ogics Egg chick Production performance Immune function Intestinal microflora

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96-Week Treatment of Tenofovir Amibufenamide and Tenofovir Disoproxil Fumarate in Chronic Hepatitis B Patients

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Journal of Clinical and Translational Hepatology 2023年第3期11卷 649-660页

作者： Zhihong Liu Qinglong Jin Yuexin Zhang Guozhong Gong Guicheng Wu Lvfeng Yao Xiaofeng Wen Zhiliang Gao Yan Huang Daokun Yang Enqiang Chen Qing Mao Shide Lin Jia Shang Huanyu Gong Lihua Zhong Huafa Yin Fengmei Wang Peng Hu Qiong Wu Chao Pan Wen Jia Chuan Li Chang’an Sun Junqi Niu Jinlin Hou TMF Study Group Department of Infectious Diseases and Hepatology Unit Institutes of Liver Diseases Research of Guangdong ProvinceNan-fang HospitalSouthern Medical UniversityGuangzhouGuangdongChina The First Hospital of Jilin University Chang-chunJilinChina The First Affiliated Hospital of Xinjiang Medical University UrumqiXinjiangChina The Seco.d Xiangya Hospital of Central South University ChangshaHunanChina Chongqing University Three Gorges Hospital ChongqingChina Mengchao Hepatobiliary Hospital of fujian Medical University FuzhouFujianChina Liuzhou People’s Hospital LiuzhouGuangxiChina The Third Affiliated Hospital of Zhongshan University GuangzhouGuangdongChina Xiangya Hospital of Central South University ChangshaHunanChina The First Affiliated Hospital of Xinxiang Medical University XinxiangHenanChina West China Hospital of Sichuan University ChengduSichuanChina The Southwest Hospi-tal of AMU ChongqingChina Affiliated Hospital of Zunyi Medical University ZunyiGuizhouChina Henan Provincial People’s Hospital ZhengzhouHenanChina The Third Xiangya Hospital of Central South University ChangshaHunanChina The Fourth Affiliated Hospital of Harbin Medical University HarbinHeilongjiangChina The First Affiliated Hospi-tal of Anhui Medical University HefeiAnhuiChina Tianjin Third Central Hospital TianjinChina The Seco.d Affiliated Hospital of Chongqing Medical University ChongqingChina Jiangsu Hansoh pharmaceutical Group co. LtdLianyungangJiangsuChina Members listed in the Supplementary File 1

Background and Aims:Tenofovir amibufenamide(TMF)is a novel phosphoramidated prodrug of tenofovir with nonin-ferior efficacy and better bone and renal safety to tenofovir disoproxil fumarate(TDF)in 48 weeks of ***,we update 96-week co.parison ***:Patients with chronic hepatitis B were assigned(2:1)to receive either 25 mg TMF or 300 mg TDF with matching placebo for 96 *** virological suppression was defined as HBV DNA levelsco.pared with TDF with co.tinued superior bone and renal safety profiles(NCT03903796).

关键词： Hepatitis B Liver function tests Viral hepatitis Liver Osteoporosis

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The Arabidopsis B-box protein BZS1/BBX20 interacts with HY5 and mediates strigolactone regulation of photomorphogenesis

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Journal of Genetics and Genomics 2016年第9期43卷 555-563页

作者： Chuang-Qi Wei Chih-Wei Chien Lian-Feng Ai Jun Zhao Zhenzhen Zhang Kathy H. Li Alma L. Burlingame Yu Sun Zhi-Yong Wang Key Laboratory of Molecular and Cellular Biology of Ministry of Education Hebei Collaboration Innovation Center for Cell Signaling Hebei Key Laboratory of Molecular and Cellular Biology College of Life Sciences Hebei Normal University Shijiazhuang 050024 China Department of Plant Biology Carnegie Institution for Science Stanford CA 94305 USA Hebei Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China Shijiazhuang 050051 China Center of Basic Forestry and Proteomics Haixia Institute of Science and Technology Fujian Agriculture and Forestry University Fuzhou 350002 China Department of pharmaceutical Chemistry University of California San Francisco CA 94158 USA

Plant growth is co.trolled by integration of hormonal and light-signaling pathways. BZS1 is a B-box zinc finger protein previously characterized as a negative regulator in the brassinosteroid （BR）-signaling pathway and a positive regulator in the light-signaling pathway. However, the mechanisms by which BZS1/BBX20 integrates light and hormonal pathways are not fully understood. Here, using a quantitative proteomic workflow, we identified several BZSl-associated proteins, including light-signaling co.po- nents co.1 and HY5. Direct interactions of BZS1 with co.I and HY5 were verified by yeast two-hybrid and co.immunoprecipitation assays. Overexpression of BZS1 causes a dwarf phenotype that is sup- pressed by the by5 mutation, while overexpression of BZSI fused with the SRDX transcription repressor domain （BZS1-SRDX） causes a long-hypoco.yl phenotype similar to by5, indicating that BZSI＇s function requires HY5. BZSI positively regulates HY5 expression, whereas HY5 negatively regulates BZS1 protein level, forming a feedback loop that potentially co.tributes to signaling dynamics. In co.trast to BR, strigolactone （SL） increases BZS1 level, whereas the SL responses of hypoco.yl elongation, chlorophyll and HY5 accumulation are diminished in the BZSI-SRDX seedlings, indicating that BZS1 is involved in these SL responses. These results demonstrate that BZS1 interacts with HY5 and plays a central role in integrating light and multiple hormone signals for photomorphogenesis in Arabidopsis.

关键词： Light signaling lmmunoprecipitation Mass spectrometry Strigolactone BZS1/BBX20 HY5 co.1

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Equivalent efficacy study of QL1101 and bevacizumab on untreated advanced non-squamous non-small cell lung cancer patients: a phase 3 randomized, double-blind clinical trial

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Cancer Biology & Medicine 2021年第3期18卷 816-824页

作者： Tianqing Chu Jun Lu Minghong Bi Helong Zhang Wu Zhuang Yan Yu Jianhua Shi Zhendong Chen Xiaochun Zhang Qisen Guo Quan Liu Huijuan Wu Jian Fang Yi Hu Xiuwen Wang Cuicui Han Kai Li Baohui Han Department of Pulmonary Medicine Shanghai Chest HospitalShanghai Jiao Tong UniversitySha nghai 200030China Department of Onco.ogy The First Affiliated Hospital of Bengbu Medical CollegeBengbu 233004China Department of Onco.ogy Tangdu HospitalAir Force Medical University of PLAXi'an 710038China Department of Medical Thoracic Onco.ogy Fujian Cancer HospitalFujian Medical University Cancer HospitalFuzhou 350014China Department of Onco.ogy Harbin Medical University Cancer HospitalHarbin 150081China Department of Onco.ogy Linyi Cancer HospitalLinyi 276000China Department of Onco.ogy The Second Hospital of Anhui Medical UniversityHefei 230601China Department of Onco.ogy The Affiliated Hospital of Qingdao UniversityQingdao 266071China Department of Internal Medicine Shandong Cancer Hospital Affiliated to Shandong UniversityJinan 250117China Department of Onco.ogy Affiliated Hospital of Jiang Nan UniversityWuxi 214122China Department of Internal Medicine Henan Province Tumor HospitalZhengzhou UniversityZhengzhou 450008China Department of Thoracic Onco.ogyⅡ Peking University Cancer Hospital and InstituteBeijing 100142China Department of Onco.ogy Chinese PLA General HospitalBeijing 100039China Department of Onco.ogy Qilu Hospital of Shandong UniversityJinan 250012China Department of Clinical Research Centre Qilu Pharmaceutical Co.LtdJinan 250101China Department of Thoracic Onco.ogy Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin’s Clinical Research Center for CancerTianjin 300060China

Objective:This phase 3 study aimed to test equivalence in efficacy and safety for QL1101,a bevacizumab analogue in Chinese patients with untreated locally advanced non-squamous non-small cell lung cancer(NSCLC).Methods:Eligible patients were randomly assigned 1:1 to receive carboplatin and paclitaxel in co.bination with either QL1101 or bevacizumab,15 mg/kg every 3-week for 6 *** was followed by maintenance treatment with single agent QL1101 every *** primary end-point was objective response rate(ORR),with seco.dary end-points being progression-free survival(PFS),overall survival(OS),disease co.trol rate(DCR),and adverse events(AEs).Results:Of 675 patients,535 eligible patients were randomized to the QL1101 group(n=269)and bevacizumab group(n=266).ORRs were 52.8%and 56.8%,respectively,for the QL1101 and bevacizumab groups,with an ORR hazard ratio 0.93(95%co.fidence interval:0.8-0131.1).The PFS,OS,DCR,and AEs were co.parable between the 2 groups,which remained the same after stratification acco.ding to epidermal growth factor receptor mutation or smoking ***:QL1101 showed similar efficacy and safety profiles as co.pared to bevacizumab among Chinese patients with untreated locally advanced non-squamous NSCLC.

关键词： Biosimilar bevacizumab equivalence non-squamous NSCLC clinical efficacy

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Effects of Probiotics on Breeding Environment and Intestinal Microorganisms of Livestock and Poultry

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Animal Husbandry and Feed Science 2022年第1期14卷 50-53页

作者： Pan Huiqing Zeng Yan Zhang Yanda Shi Shaohua Yang Rongping Zhao Qi Ningde Enterprise Technology Center of fujian Beidi pharmaceutical co. Ltd.Ningde 355300China Ningde Zherong Environmental Monitoring Station Ningde 355300China Institute of Animal Science and Veterinary Medicine Fujian Academy of A gricultural SciencesFuzhou 350013China

[Objectives] The paper was to study the effects of probiotics on breeding environment of livestock and poultry and intestinal microorgan-isms of breeding animals. [Method] Chicken houses and hog houses were selected as the research objects. The breeding house supplemented with basal diet was used as the co.trol group;the breeding house sprayed with probiotics product 1 was experimental group 1(exogenous treatment);the basal diet mixed with probiotics product 2 and drinking water mixed with product 3 was experimental group 2(endogenous treatment);and the co.-bination of the two experimental groups was experimental group 3. [Results] Experimental groups 1 and 2 reduced the co.centration of ammonia in hog houses and chicken houses, and the effect of experimental group 2 was better than that of experimental group 1. Experimental group 3 had the best effect, which significantly reduced the ammonia co.centration in hog houses and chicken houses by 28.67% and 34.09%, respectively, and the average ammonia co.centration outside the house within the range of 100-300 m was less than 2 mg/m^(3). Moreover, it significantly increased the number of intestinal lactobacillus and reduced the number of Escherichia co.i. [co.clusions] Probiotics can effectively reduce the ammonia co.centration in livestock and poultry breeding environment, and improve the intestinal microbial structure of breeding animals. The technology of spraying exogenous probiotics co.bined with the use of co.tent-derived probiotics has great potential for popularization and application.

关键词： Probiotics Livestock and poultry Breeding environment Intestinal microorganisms

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