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全人源抗狂犬病病毒糖蛋白抗体的制备及鉴定

南京医科大学学报（自然科学版） 2017年第2期37卷 160-164页

作者：王晓蕾朱进哈卓张晓萍杨岚杨晓明刘云成 Mason Lu 冯振卿南京医科大学病理学系江苏南京210029 南京医科大学卫生部抗体技术重点实验室江苏南京210029 南京医科大学附属南京医院病理科江苏南京210006 南京军区军事医学研究所江苏南京210002 东竺明生物技术有限公司黑龙江大庆163316 福瑞邦生物科技有限公司黑龙江大庆163316 University of Texas Md Anderson Cancer center HoustonTexas 77030

目的:利用重组狂犬病病毒糖蛋白(rVG)免疫人源Ig M转基因小鼠,制备全人源抗重组rVG蛋白单克隆抗体,并对其免疫学特性进行初步鉴定。方法:以重组rVG蛋白作为抗原免疫人源Ig M转基因小鼠,采用杂交瘤技术制备筛选全人源抗重组rVG蛋白杂交... 详细信息

目的:利用重组狂犬病病毒糖蛋白(rVG)免疫人源Ig M转基因小鼠,制备全人源抗重组rVG蛋白单克隆抗体,并对其免疫学特性进行初步鉴定。方法:以重组rVG蛋白作为抗原免疫人源Ig M转基因小鼠,采用杂交瘤技术制备筛选全人源抗重组rVG蛋白杂交瘤细胞株,双抗体夹心ELISA实验鉴定单抗的人源性及抗体类型,并对其特异性及与灭活狂犬病病毒CVS-11株的结合能力进行鉴定。结果:建立了5株稳定分泌抗重组rVG的全人源单克隆抗体杂交瘤细胞株,分别命名为5d1、6H11、9A3、15d6、19E6,均为人源Ig M免疫球蛋白,5株单抗均能特异性识别重组rVG蛋白,其中3株能与灭活狂犬病病毒CVS-11株特异性结合。结论:筛选制备了特异性全人源抗重组rVG蛋白的单克隆抗体,能与灭活狂犬病病毒CVS-11株特异性结合,为进一步研制用于狂犬病防治的抗体药物奠定了基础。

关键词：人源Ig M转基因小鼠狂犬病病毒糖蛋白全人源单克隆抗体

缝隙连接偶联赋予星形胶质细胞合胞体以等电位

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神经损伤与功能重建 2016年第2期11卷 148-148页

作者： Ma B Buckalew r du Y Kiyoshi CM Alford CC Wang W McTigue dM Enyeart JJ Terman d Zhou M 王晶 department of Neuroscience the Ohio State University Wexner Medical Center Columbus Ohio 43210. Mathematical Biosciences Institute the Ohio State University Columbus Ohio 43210. department of Mathematics the Ohio State University Columbus Ohio 43210.

星形胶质细胞(星胶)通过缝隙连接广泛地偶联为合胞体。然而这一重要的脑内网络系统的基本作用尚不明确。本研究结合电生理和计算机模型方法对该问题进行了探讨.当部分或完全替换记录电极液中钾离子致钾平衡电位偏离生理电位时,单个新鲜... 详细信息

星形胶质细胞(星胶)通过缝隙连接广泛地偶联为合胞体。然而这一重要的脑内网络系统的基本作用尚不明确。本研究结合电生理和计算机模型方法对该问题进行了探讨.当部分或完全替换记录电极液中钾离子致钾平衡电位偏离生理电位时,单个新鲜分离海马星胶膜电位出现预期的去极化,而记录到的海马合胞体中个体星胶的膜电位依然呈现在准生理水平。进一步研究表明,这种表观异常的膜电位反应是因为合胞体中星胶之间存在的强大的电偶联和离子偶联,偶联使任何个体星胶的膜电位趋向与周围细胞均衡相等。在功能上,这种合胞体等电位效应能最大程度减小因局部细胞外钾水平升高引起的膜电位的去极化,从而为高效的钾摄取保持持续的驱动力。因此,星胶之间缝隙连接偶联的功能目的是使合胞体达到等电位,由此为神经环路的关键功能提供稳定的细胞外环境。

关键词： K+清除率偶联系数电偶联膜电位

diterpenoids from Scutellaria barbata induce tumour-selective cytotoxicity by taking the brakes off apoptosis

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Medicinal Plant Biology 2022年第1期1卷 8-23页

作者： Matthew L.Tomlinson Man Zhao Elaine J.Barclay Jie Li Haixiu Li Juri Felix Lionel Hill Gerhard Saalbach Martin rejzek dongfeng Yang Qing Zhao Paul Kroon Wei Wang Yongping Bao Melanie-Jayne r.Howes Evangelos C.Tatsis Cathie Martin The John Innes Centre NorwichNR47UHUnited Kingdom College of Bioengineering and Biotechnology Zhejiang University of TechnologyHangzhou 310058China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China royal Botanic Gardens Kew RichmondSurreyLondon TW93ABUnited Kingdom College of Life Sciences Zhejiang Sci-Tech UniversityXiasha Higher Education ZoneHangzhou 310018China Shanghai Key Laboratory of Plant Functional Genomics and resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai 201602China Quadram Institute Bioscience Norwich Research ParkNorwichNR47UAUnited Kingdom Norwich Medical School University of East AngliaNorwichNorfolkNR47TJUnited Kingdom CEPAMS:CAS-JIC Centre of Excellence in Plant and Microbial Sciences

Medicinal plants are an excellent source of structurally diverse,bio-active compounds with potential in the fight against *** of the most promising is Scutellaria barbata,prescribed traditionally for the treatment of *** A is the major diterpenoid,produced in specialized large,peltate trichomes on leaves of *** induces dose-dependent apoptosis,specifically in cancer *** major class of proteins down-regulated are pro-survival proteins,the Inhibitors of Apoptosis(IAPs),and IAP regulating *** propose that scutebarbatine A works by releasing the molecular brakes(the IAPs)on apoptosis in cell death-evading cancer *** between the cytotoxicity of methanolic extracts of *** leaves and decoctions(Ban Zhi Lian)prepared traditionally,showed substantially different chemical compositions and differential induction of *** suggest polyvalency between the constituents in both extracts,and ways to produce enhanced chemopreventive preparations for the treatment of cancer.

关键词： prescribed methanol structurally

经医疗保健代理人调查耶和华见证人会孕妇对应用血制品的反应

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世界核心医学期刊文摘（妇产科学分册） 2005年第9期1卷 48-48页

作者： Gyamfi C. Berkowitz r.L. 杨晓梅 division of Maternal-Fetal Medicine Dept. Obstet. Gynecol./Repro. Sci. Mount Sinai Medical Center 5 East 98th St reet New York NY 10029 United States Dr.

OBJECTIVE: To review the treatment options presented on die New York State H ea lth Care Prosy for Jehovah’s Witnesses, which is signed by pregnant women when they present for care. METHOdS: Chart reviews were performed for all women who p resented to labor and delivery at our institution from 1997 to 2002 and identifi ed themselves as Jehovah’s Witnesses. A patient was included in the study if a completed health care proxy was available in her chart data were derived from th e health care prosy and from the Mount Sinai School of Medicine’s Blood Product Checklist for Jehovah’s Witness Patients. Variables of interest included age, race, parity, and antenatal and perinatal complications. rESULTS: A total of 61 patients were identified. Of these, 39.3%agreed to accept a variety of donated blood products, 9.8%would accept donated packed red blood cells, and 50.1%woul d accept neither from a homologous donor. With respect to nonstored autologous b lood, 55%of respondents would accept either intraoperative normovolemic hemodil ution or transfusion of their own blood obtained by a cell salvage system. No si gnificant differences in responses were noted for any of the above-mentioned va riables. CONCLUSION: This review refutes the commonly held belief that all Jehov ah’s Witnesses refuse to accept blood or any of its products. In this populatio n of pregnant women, the majority were willing to accept some form of blood or b lood products. This information can be used to help health care providers counse l a patient when she is initially faced with considering these issues and may he lp to remove the stigma of accepting one of the options.

关键词：医疗保健血制品自体血浓缩红细胞图表分析围生期出生前蒙特纽约州无显著性差异

LFA-1 antagonist (BIrT377) similarly reverses peripheral neuropathic pain in male and female mice with underlying sex divergent peripheral immune proinflammatory phenotypes

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Neuroimmunology and Neuroinflammation 2019年第7期6卷 12-43页

作者： Shahani Noor Melody S.Sun Arden G.Vanderwall Mara A.Havard Jacob E.Sanchez Nathan W.Harris Monique V.Nysus Jeffrey P.Norenberg Harrison T.West Carsten r.Wagner Lauren L.Jantzie Nikolaos Mellios Erin d.Milligan department of Neurosciences School of MedicineUniversity of New MexicoAlbuquerqueNM 87131USA department of Anesthesiology and Critical Care University of New MexicoAlbuquerqueNM 87131USA department of Pediatrics and Neurology Johns Hopkins University School of MedicineBaltimoreMD 21205-2196USA department of radiopharmaceutical Sciences College of PharmacyNew Mexico Center for Isotopes in MedicineUniversity of New MexicoAlbuquerqueNM 87131USA department of Medicinal Chemistry University of MinnesotaCollege of PharmacyMinneapolisMN 55455USA

Aim:The majority of preclinical studies investigating aberrant glial-neuroimmune actions underlying neuropathic pain have focused on male rodent ***,studies have shown peripheral immune cells play a more prominent role than glial cells in mediating pathological pain in ***,we compared the onset and duration of allodynia in males and females,and the anti-allodynic action of a potentially novel therapeutic drug(BIrT377)that not only antagonizes the action of lymphocyte function-associated antigen-1(LFA-1)to reduce cell migration in the periphery,but may also directly alter the cellular inflammatory ***:Male and female mice were subjected to peripheral nerve injury chronic constriction injury(CCI)applying two methods,using either 4-0 or 5-0 chromic gut suture material,to examine potential sex differences in the onset,magnitude and duration of *** sensitivity before and after CCI and application of intravenous BIrT377 was *** and spinal tissues were analyzed for protein(multiplex electrochemiluminescence technology)and mrNA expression(quantitative real-time PCr).The phenotype of peripheral T cells was determined using flow ***:Sex differences in proinflammatory CCL2 and IL-1βand the anti-inflammatory IL-10 were observed from a set of cytokines analyzed.A profound proinflammatory T cell(Th17)response in the periphery and spinal cord was also observed in neuropathic ***377 reversed pain,reduced IL-1βand TNF,and increased IL-10 and transforming growth factor(TGF)-β1,also an anti-inflammatory cytokine,in both ***,female-derived T cell cytokines are transcriptionally regulated by BIrT377,as demonstrated by reducing proinflammatory IL-17A production with concurrent increases in IL-10,TGF-β1 and the anti-inflammatory regulatory T cell-related factor,***:This study supports that divergent peripheral immune and neuroimmune responses during neuropathy exists between males and

关键词： Neuropathic pain glia neuroimmune peripheral immune T cells

A paratlei mechanism underlying frizzle in domestic chickens

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Journal of Molecular cell Biology 2018年第6期10卷 589-591页

作者： Xing Guo Yan-Qing Li Ming-Shan Wang Zhi-Bin Wang Quan Zhang Yong Shao run-Shen Jiang Sheng Wang Chen-dong Ma robert W.Murphy Guang-Qin Wang Jing dong Li Zhang dong-dong Wu Bing-Wang du Min-Sheng Peng Ya-Ping Zhang State Key Laboratory of Genetic resources and Evolution and Yunnan Laboratory of Molecular Biology of domestic Animals Kunmin8 Institute of ZoologyChinese Academy of SciencesKunming 650223 China College of Agricultural Guangdong Ocean UniversityZhanjiang 524088China College of Animal Science and Technology Anhui Agricultural UniversityHefei 230036 China Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture of ChinaNational Engineering Laboratory for Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193 China Centre for Biodiversity and Conservation Biology Royal Ontario MuseumTorontoM5S2C6Canada 6Jinsheng Animal Husbandry Technology Co. Ltd Zhanjiang 524025China Kunming College of Life Science University of Chinese Academy of SciencesKunming 650204China center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences Kunming 650223China State Key Laboratory for Conservation and Utilization of Bio-resources Yunnan University Kunming 650091China

dear Editor,The feather,a highly keratinized tissue with variations in the shape,distribution,pigmentation,and structure,is an attractive topic in developmental and evolutionary biology (Boer et al.,2017).

关键词： evolutionary biology domestic chickens

Etoposide,dexamethasone,and pegaspargase with sandwiched radiotherapy in early-stage natural killer/T-cell lymphoma:A randomized phase III study

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The Innovation 2023年第3期4卷 70-78页

作者： Huijuan Zhong Shu Cheng Xi Zhang Bing Xu Jiayi Chen Xufeng Jiang Jie Xiong Yu Hu Guohui Cui Juying Wei Wenbin Qian Xiaobing Huang Ming Hou Feng Yan Xin Wang Yongping Song Jianda Hu Yuanhua Liu Xuejun Ma Fei Li Chongyang Wu Junmin Chen Li Yu Ou Bai Jingyan Xu Zunmin Zhu Li Liu Xin Zhou Li Huang Yin Tong Ting Niu depei Wu Hao Zhang Chaofu Wang Binshen Ouyang Hongmei Yi Qi Song Gang Cai Biao Li Jia Liu Zhifeng Li rong Xiao Luqun Wang Yujie Jiang Yanyan Liu Xiaoyun Zheng Pengpeng Xu Hengye Huang Li Wang Saijuan Chen Weili Zhao Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China department of Hematology Xinqiao HospitalChongqing 400037China department of Hematology First Affiliated Hospital of Xiamen UniversityXiamenFujian 361009China department of radiation Oncology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China department of Nuclear Medicine Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China department of Hematology First Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310003China department of Hematology Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310009China Institute of Hematology Department of HematologySichuan Provincial People’s HospitalChengduSichuan 610072China department of Hematology Shandong Provincial Key Laboratory of ImmunohematologyQilu HospitalShandong UniversityJinanShandong 250012China department of Hematology Third Affiliated Hospital of Suzhou UniversityFirst People’s Hospital of ChangzhouChangzhouJiangsu 213004China department of Hematology Shandong Provincial Hospital Affiliated to Shandong UniversityJinanShandong 250021China department of Hematology Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenan 450003China Fujian Institute of Hematology Fujian Medical University Union HospitalFuzhouFujian 350401China department of Medical Oncology Jiangsu Cancer HospitalNanjingJiangsu 210009China department of Medical Oncology Shanghai Cancer CenterFudan UniversityShanghai 200032China department of Hematology First Affiliated Hospital of Nanchang UniversityNanchan

Methotrexate,etoposide,dexamethasone,and pegaspargase(MESA)with sandwiched radiotherapy is known to be effective for early-stage extranodal natural killer/T-cell lymphoma,nasal type(NKTCL).We explored the efficacy and safety of reduced-intensity,non-intravenous etoposide,dexamethasone,and pegaspargase(ESA)with sandwiched *** multicenter,randomized,phase III trial enrolled patients aged between 14 and 70 years with newly diagnosed early-stage nasal NKTCL from 27 centers in *** were randomly assigned(1:1)to receive ESA(pegaspargase 2,500 IU/m^(2)intramuscularly on day 1,etoposide 200 mg orally,and dexamethasone 40 mg orally on days 2–4)or MESA(methotrexate 1 g/m^(2)intravenously on day 1,etoposide 200 mg orally,and dexamethasone 40 mg orally on days 2–4,and pegaspargase 2,500 IU/m^(2)intramuscularly on day 5)regimen(four cycles),combined with sandwiched radiotherapy.

关键词： radiotherapy killer lymphoma

Nat Genet:单基因突变导致过敏性皮炎的发生

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现代生物医学进展 2017年第27期17卷 I0004-I0004页

作者： Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas diMaggio, Kelly d Stone, Joshua d Milner Jeffrey r Stinson, Elisa ruffo, Batsukh dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul r reynolds, Erwin W Gelfand Elisa ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio d rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea r Bernasconi, Geronimo dubra, Silvia danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil romberg Laboratory of Allergic diseases National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda Maryland USA department of Pharmacology and Molecular Therapeutics Uniformed Services University of the Health Sciences Bethesda Maryland USA Immunodeficiency diagnosis and Treatment Program Department of Pediatrics National Jewish Health Denver Colorado USA department of Translational Medicine University of Piemonte Orientale Novara Italy department of Immunobiology Yale University School of Medicine New Haven Connecticut USA Immunology Service Department of Laboratory Medicine NIH Clinical Center National Institutes of Health Bethesda Maryland USA Human Immunological disease Section National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda Maryland USA Merck research Laboratories Merck and Co. Inc. Boston Massachusetts USA Clinical research directorate/Clinical Monitoring research Program Leidos Biomedical Research Inc. NCI Campus at Frederick Frederick Maryland USA Servicio de Immunología y reumatología Hospital Nacional de Pediatría Prof. Dr. Juan P. Garrahan Buenos Aires Argentina division of dermatology Department of Medicine Washington University School of Medicine St. Louis Missouri USA department of Pediatrics Division of Rheumatology and Department of Pathology and Immunology Washington University School of Medicine St. Louis Missouri USA department of Pediatrics Children’s Hospital of Philadelphia Philadelphia Pennsylvania USA.

最近，研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变：CArdll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析，发现了这一导致疾病产生的基因、

关键词：过敏性皮炎基因突变 Nat 神经性皮炎血缘关系研究所传染病 NIH