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Journal of Integrative Agriculture 2018年第5期17卷 1032-1039页

作者： YU Tong-ying LU Ming-xing CUI Ya-dong biology and Food Engineering School Fuyang Normal University School of Horticulture and Plant Protection & institute of applied Entomology Yangzhou University

Many proteins require assistance from molecular chaperones at various stages to attain correctly folded states and functional conformations during protein synthesis. In this study, the gene encoding T-complex polypeptide 1（TCP-1）, which belongs to the heat shock protein 60（HSP60） family, was isolated and characterized from the rice stem borer, Chilo suppressalis, by RACE and q PCR, respectively. The full-length c DNA of Tcp-1 was 2 144 bp and encoded a 1 635-bp ORF; the deduced translational product contained 545 amino acids with 5′-and 3′-UTRs and an isoelectric point of 5.29. Cluster analysis confirmed that the deduced amino acid sequence shared high identity（60–99%） with TCP-1 from other insects. To investigate Tcp-1 expression in response to abiotic stress, q PCR was used to analyze expression levels of Tcp-1 m RNA in C. suppressalis larvae exposed to temperatures ranging from –11 to 43°C. With respect to heat shock, Tcp-1 expression was higher than the control after a 2-h exposure to 30 and 36°C and declined at 39 and 43°C. Difference in Tcp-1 expression was observed at temperatures ranging from –11 to 27°C. q PCR analyses revealed that Tcp-1 expression was the highest in hindgut tissue as compared to heads, epidermis, fat body, foregut, midgut, and malpighian tubules. Our results indicated that Tcp-1 expression was differentially expressed in C. suppressalis tissues, and was impacted by temperature stress.

关键词： TCP-1 Chilo suppressalis expression analysis temperature stress insect tissues

Configuration interaction studies on the spectroscopic properties of PbO including spin-orbit coupling

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Chinese Physics B 2016年第7期25卷 185-191页

作者：罗旺李瑞盖志强艾瑞波张宏民张晓美闫冰 College of Science Qiqihar University institute of Atomic and molecular Physics Jilin University Department of Electronic Information Jiangsu University of Science and Technology Jilin Provincial Key Laboratory of applied Atomic and molecular Spectroscopy Jilin University

Lead oxide（Pb O）, which plays the key roles in a range of research fields, has received a great deal of attention. Owing to the large density of electronic states and heavy atom Pb including in Pb O, the excited states of the molecule have not been well studied. In this work, high level multireference configuration interaction calculations on the low-lying states of Pb O have been carried out by utilizing the relativistic effective core potential. The effects of the core-valence correlation correction, the Davidson modification, and the spin–orbital coupling on the electronic structure of the Pb O molecule are estimated. The potential energy curves of 18 Λ-S states correlated to the lowest dissociation limit（Pb（~3P_g）＋ O（~3P_g）） are reported. The calculated spectroscopic parameters of the electronic states below 30000 cm^（-1）, for instance, X^1Σ~＋, 1~3Σ~＋,and 1~3Σ^-, and their spin–orbit coupling interaction, are compared with the experimental results, and good agreements are derived. The dipole moments of the 18 Λ-S states are computed with the configuration interaction method, and the calculated dipole moments of X^1Σ~＋and 1~3Σ~＋are consistent with the previous experimental results. The transition dipole moments from 1~1Π, 2~1Π, and 2~Σ to X^1Σ~＋and other singlet excited states are estimated. The radiative lifetime of several low-lying vibrational levels of 1~1Π, 2~1Π, and 2~1Σ~＋ states are evaluated.

关键词： lead oxide MRCI+Q method dipole moments transitional properties

Machine-learning assisted design principle search for singlet fission:an example study of cibalackrot

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npj Computational Materials 2022年第1期8卷 1703-1716页

作者： Fabian Weber Hirotoshi Mori Department of applied Chemistry Chuo University112-8551 Tokyo-toBunkyo-kuKasuga1-13-27Japan Department of Theoretical and Computational molecular Science Institute for Molecular ScienceOkazaki444-8585Japan

This work uses quantum chemistry calculations and machine learning to explore design rules for singlet fission in a chemical space of four million indigoid *** identify~400,000 derivatives of 2,2′-diethenyl cibalackrot,which theoretically fulfil the energy conditions for exoergic singlet fission above the silicon band gap *** this database with a random forest classifier,we observe that small substituents with positive mesomeric effects and weak negative inductive effects reinforce the desired energetic conditions when placed at specific ***,a subset of molecules that reflects the random forest classifier’s rules are investigated for their quantum chemical properties to translate the desirable structural motifs into wavefunction-based design ***,direct correlations between the energetic condition for singlet fission,the biradical character and the charge and triplet spin density in prominent molecular regions are identified,providing insights that may serve as a guide for singlet fission core structure development.

关键词： singlet quantum classifier

Improving the bioavailability of pharmacologically active substances in pharmaceutical and cosmetic formulations

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 33-34页

作者： Joachim Storsberg Marcel W.Laughton Martin Geyer Mont Kumpugdee-Vollrath Christian Schmidt fraunhofer institute applied Polymer Research(IAP) 14476 PotsdamGermany Beuth University applied Sciences 13353 BerlinGermany

One and the same active ingredient can be used in different pharmaceutical applications to treat and cure ***,the active ingredient can be used also in cosmetic formulations to care or aid the healing *** applied topically via creams or lotions,they are referred as *** general,one aims to improve the bioavailability through tailored formulations and careful selection of *** example is the use of latanoprost in ophthalmology for lowering the intraocular pressure in glaucoma patients with side effects including stimulated growth of *** this open up the possibility of using latanoprost for the local treatment for hair loss?

关键词： Drug delivery Bioavailability Nano carriers Cosmeceutical Galenicals

Phylogenetic, phylogeographic and divergence time analysis of Anopheles subpictus species complex using ITS2 and COI sequences

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Asian Pacific Journal of Tropical Medicine 2024年第5期17卷 214-225,I0004-I0038页

作者： Lihini Sandaleka Muthukumarana Methsala Madurangi Wedage Samanthika Rathnayake Nissanka Kolitha De Silva Department of Zoology Faculty of Applied SciencesUniversity of Sri JayewardenepuraGangodawilaNugegodaSri Lanka Genetics and molecular biology Unit Faculty of Applied SciencesUniversity of Sri JayewardenepuraGangodawilaNugegodaSri Lanka Sri Lanka institute of Biotechnology PitipanaHomagamaSri Lanka

Objective:To address the phylogenetic and phylogeographic relationship between different lineages of Anopheles(An.)subpictus species complex in most parts of the Asian continent by maximum utilization of Internal Transcriber Spacer 2(ITS2)and cytochrome C oxidase I(COI)sequences deposited at the ***:Seventy-five ITS2,210 COI and 26 concatenated sequences available in the NCBI database were *** analysis was performed using Bayesian likelihood trees,whereas median-joining haplotype networks and time-scale divergence trees were generated for phylogeographic *** diversity indices and genetic differentiation were also ***:Two genetically divergent molecular forms of *** species complex corresponding to sibling species A and B are *** A evolved around 37-82 million years ago in Sri Lanka,India,and the Netherlands,and species B evolved around 22-79 million years ago in Sri Lanka,India,and ***,Thailand,and Cambodia have two molecular forms:one is phylogenetically similar to species *** forms differ from species A and B and evolved recently in the above mentioned countries,Indonesia and the *** subdivision among Sri Lanka,India,and the Netherlands is almost absent.A substantial genetic differentiation was obtained for some populations due to isolation by large geographical *** diversity indices reveal the presence of a long-established stable mosquito population,at mutation-drift equilibrium,regardless of population ***:*** species complex consists of more than two genetically divergent molecular *** A is highly divergent from the *** Lanka and India contain only species A and B.

关键词： molecular systematics ITS2 COI DNA sequences Phylogeny Phylogeography

Atomic Structures and Radiative Properties of He-like Ions in Debye Plasmas

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Plasma Science and Technology 2010年第3期12卷 372-377页

作者：李博文董晨钟蒋军王建国 College of Physics and Electronic Engineering Northwest Normal University Joint Laboratory of Atomic and molecular Physics Northwest Normal University & Institute of Modern PhysicsCAS institute of applied Physics and Computational Mathematic

A detailed investigation of plasma screening effects on atomic structure and transition properties are presented for He-like ions embedded in dense plasma environment. Multiconfiguration Dirac-Fock calculations were carried out for these ions by considering a Debye-H/ickel potential. A large-scale relativistic configuration sition energies and transition probabilities and interaction method is adopted to calculate tranto allow for a systematic improvement of the calculations. Comparison of the presently calculated results with others, when available, is made

关键词： Debye plasmas MCDF electron correlation

TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants

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molecular Plant 2020年第5期13卷 717-731页

作者： Jeanette Moulinier-Anzola Maximilian Schwihla Lucinda De-Araujo Christina Artner Lisa Jorg Nataliia Konstantinova Christian Luschnig Barbara Korbei institute of molecular Plant biology Department of Applied Genetics and Cell BiologyUniversity of Natural Resources and Life SciencesVienna(BOKU)Muthgasse 181190 ViennaAustria Department of molecular Toxicology and Environment Biotechnology Center-Eduardo Mondlane UniversityAv.de MocambiqueKm 1.5.MaputoMozambique institute of Science and Technology Austria(1ST Austria) Am Campus 13400 KlosterneuburgAustria

Protein abundance and localization at the plasma membrane(PM)shapes plant development and mediates adaptation to changing environmental *** is regulated by ubiquitination,a post-translational modification crucial for the proper sorting of endocytosed PM proteins to the vacuole for subsequent *** understand the significance and the variety of roles played by this reversible modification,the function of ubiquitin receptors,which translate the ubiquitin signature into a cellular response,needs to be *** this study,we show that TOL(TOM1-like)proteins function in plants as multivalent ubiquitin receptors,governing ubiquitinated cargo delivery to the vacuole via the conserved Endosomal Sorting Complex Required for Transport(ESCRT)***2 and TOL6 interact with components of the ESCRT machinery and bind to K63-linked ubiquitin via two tandemly arranged conserved ubiquitin-binding *** of these domains results not only in a loss of ubiquitin binding but also altered localization,abolishing TOL6 ubiquitin receptor *** and localization of TOL6 is itself regulated by ubiquitination,whereby TOL6 ubiquitination potentially modulates degradation of PM-localized cargoes,assisting in the fine-tuning of the delicate interplay between protein recycling and *** together,our findings demonstrate the function and regulation of a ubiquitin receptor that mediates vacuolar degradation of PM proteins in higher plants.

关键词： ubiquitination ESCRT pathway ubiquitin receptor plasma membrane protein degradation

Parasitoid wasps as effective biological control agents

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Journal of Integrative Agriculture 2019年第4期18卷 705-715页

作者： WANG Zhi-zhi LIU Yin-quan SHI Min HUANG Jian-hua CHEN Xue-xin Ministry of Agriculture Key Lab of molecular biology of Crop Pathogens and Insects Zhejiang University State Key Lab of Rice biology Zhejiang University institute of Insect Sciences Zhejiang University

Biological control(biocontrol) is a safe, sustainable approach that takes advantage of natural enemies such as predators, parasitic insects or pathogens to manage pests in agroecosystems. Parasitoid wasps, a very large evolutionary group of hymenopteran insects, are well-known biological control agents for arthropod pests in agricultural and forest ecosystems. Here, we summarize the recent progress on the application of parasitoid wasps in biocontrol in China for the last five years. These include species diversity of parasitoid wasps, identification of dominant parasitoid wasps associated with insect pests and biocontrol practices(three types of biological control, i.e., classical, augmentative and conservation biological control) in several Chinese agroecosystems. We then treat different mass-rearing and release technologies and the commercialization of several parasitoid wasp species. We also summarize other work that may have a potential use in biocontrol, including the effect of plant volatiles on parasitoids and recent advance in the molecular mechanisms underlying the host regulation by parasitoid wasps. Future research area and applied perspectives are also discussed, noting that advances in biocontrol technologies in Chinese agriculture informs research at the global level.

关键词： parasitoid wasp predator biological control/biocontrol biological control/biocontrol agent cereal crop vegetable crop mass-rearing field release plant volatile parasitoid-host interaction

Influence of target temperature on femtosecond laser-ablated brass plasma spectroscopy

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Plasma Science and Technology 2020年第7期22卷 4-10页

作者： Junfeng SHAO Jin GUO Qiuyun WANG Anmin CHEN Mingxing JIN institute of Atomic and molecular Physics Jilin UniversityChangchun 130012People's Republic of China Jilin Provincial Key Laboratory of applied Atomic and molecular Spectroscopy(Jilin University) Changchun 130012People’s Republic of China

Spectral intensity,electron temperature and density of laser-induced plasma(LIP) are important parameters for affecting sensitivity of laser-induced breakdown spectroscopy(LIBS).Increasing target temperature is an easy and feasible method to improve the *** this paper,a brass target in a temperature range from 25℃ to 200℃ was ablated to generate the LIP using femtosecond ***-resolved spectral emission of the femtosecond LIBS was measured under different target *** results showed that,compared with the experimental condition of 25℃,the spectral intensity of the femtosecond LIP was enhanced with more temperature *** addition,the electron temperature and density were calculated by Boltzmann equation and Stark broadening,indicating that the changes in the electron temperature and density of femtosecond LIP with the increase of the target temperature were different from each *** increasing the target temperature,the electron temperature increased while the electron density ***,in femtosecond LIBS,a hightemperature and low-density plasma with high emission can be generated by increasing the target *** increase in the target temperature can improve the resolution and sensitivity of femtosecond LIBS.

关键词： laser-induced breakdown spectroscopy time-resolved spectroscopy emission enhancement femtosecond laser target temperature

Time-resolved spectroscopy of femtosecond laser-induced Cu plasma with spark discharge

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Plasma Science and Technology 2019年第6期21卷 140-146页

作者： Qiuyun WANG Anmin CHEN Wanpeng XU Dan ZHANG Ying WANG Suyu LI Yuanfei JIANG Mingxing JIN institute of Atomic and molecular Physics Jilin University Changchun 130012 People's Republic of China Jilin Provincial Key Laboratory of applied Atomic and molecular Spectroscopy(Jilin University) Changchun 130012 People's Republic of China

The combination of spark discharge and laser-induced breakdown spectroscopy (LIBS) is called spark discharge assisted *** works under laser-plasma triggered spark discharge mode,and shows its ability to enhance spectral emission *** work uses a femtosecond laser as the light souuce,since femtosecond laser has many advantages in laser-induced plasma compared with nanosecond laser,meanwhile,the study on femtosecond LIBS with spark discharge is ***-resolved spectroscopy of spark discharge assisted femtosecond LIBS was investigated under different discharge voltages and laser *** results showed that the spectral intensity was significantly enhanced by using spark discharge compared with LIBS ***,the spectral emission intensity using spark discharge assisted LIBS increased with the increase in the laser *** addition,at low laser energy,there was an obvious delay on the discharge time compared with high laser energy,and the discharge time with positive voltage was different from that with negative voltage.

关键词： laser-induced breakdown spectroscopy femtosecond laser spark discharge emission enhancement