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Evaluation of IFIT3 and ORM1 as Biomarkers for Discriminating Active tuberculosis from Latent Infection

Current Medical Science 2022年第6期42卷 1201-1212页

作者： Bing-fen YANG Fei ZHAI Shan YU Hong-juan AN Zhi-hong CAO Yan-hua LIU Ruo WANG Xiao-xing CHENG tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and TreatmentInstitute of Senior Department of Tuberculosisthe Eighth Medical Center of Chinese PLA General HospitalBeijing100091China fourth Division Senior Department of Tuberculosisthe Eighth Medical Center of PLA General HospitalBeijing100091China department of tuberculosis Prevention and Control Senior Department of Tuberculosisthe Eighth Medical Center of PLA General HospitalBeijing100091China

Objective Current commercially available immunological tests cannot be used for discriminating active tuberculosis(TB)from latent TB *** evaluate the value of biomarker candidates in the diagnosis of active TB,this study aimed to identify differentially expressed genes in peripheral blood mononuclear cells(PBMCs)between patients with active TB and individuals with latent TB infection by transcriptome *** The differentially expressed genes in unstimulated PBMCs and in Mycobacterium tuberculosis(Mtb)antigen-stimulated PBMCs from patients with active TB and individuals with latent TB infection were identified by transcriptome *** candidate genes were evaluated in cohorts consisting of 110 patients with TB,30 individuals with latent TB infections,and 50 healthy controls by quantitative real-time *** operating characteristic(ROC)curve analysis was performed to calculate the diagnostic value of the biomarker *** Among the differentially expressed genes in PBMCs without Mtb antigen stimulation,interferon-induced protein with tetratricopeptide repeats 3(IFIT3)had the highest area under curve(AUC)value(0.918,95%CI:0.852-0.984,P<0.0001)in discriminating patients with active TB from individuals with latent TB infection,with a sensitivity of 91.86%and a specificity of 84.00%.In Mtb antigen-stimulated PBMCs,orosomucoid 1(ORM1)had a high AUC value(0.833,95%CI:0.752-0.915,P<0.0001),with a sensitivity of 81.94%and a specificity of 70.00%.Conclusion IFIT3 and ORM1 might be potential biomarkers for discriminating active TB from latent TB infection.

关键词： tuberculosis biomarker latent tuberculosis infection interferon-induced protein with tetratricopeptide repeats 3 orosomucoid 1

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Diagnostic performance and problem analysis of commercial tuberculosis antibody detection kits in China

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Military Medical Research 2018年第3期5卷 242-250页

作者： Xue-Juan Bai You-Rong Yang Jian-Qin Liang Hui-Ru An Jie Wang Yan-Bo Ling Zhong-Yuan Wang Xue-Qiong Wu Army tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment Institute of Tuberculosis Research 309 Hospital of Chinese PLA tuberculosis department No.2.Institute of tuberculosis Research 309 hospital of Chinese PLA tuberculosis department No.1.Institute of tuberculosis Research 309 hospital of Chinese PLA tuberculosis department No.3 Institute of Tuberculosis Research 309 Hospital of Chinese PLA

Background: The diagnosis of bacterium-negative pulmonary tuberculosis(TB) and extra-pulmonary TB is challenging clinically. The detection of the anti-TB antibody has an important, auxiliary, clinical diagnostic value. Therefore, TB antibody detection kits should be screened and evaluated, and the reagents with the highest sensitivity and specificity should be chosen and used ***: The diagnostic performance of 7 commercially available TB antibody detection kits(kits A, B, C, D, E, F and G) based on the gold immunoassay detection of immunoglobulin(Ig) G or IgM antibodies were simultaneously evaluated and compared in 62 TB cases and 56 non-TB cases in a laboratory. A retrospective analysis including 2549 cases was carried out to assess the clinical diagnosis values of bacteriological examinations and TB antibody tests(kits B and H used in the clinic).Results: The sensitivities of TB antibody kits A, B, C, D, E, F and G in the sera from 62 TB patients were 50.0%, 83.9%, 38.7%, 9.7%, 48.4%, 69.4% and 79.0%, respectively; the sensitivities in the sera from 24 smear-negative TB patients were 29.2%, 79.2%, 29.2%, 12.5%, 29.2%, 54.2% and 79.2%, respectively; the specificities in the sera from 56 nonTB patients were 73.2%, 25.0%, 85.7%, 96.4%, 78.6%, 78.6% and 50.0%, respectively. Of the 2549 clinically diagnosed cases, there were 1752 pulmonary TB cases, 505 extra-pulmonary TB cases, 87 old pulmonary TB cases and 205 non-TB cases. The positive results for smear, culture, TB antibody kit B and kit H in pulmonary TB cases were 39.8%(543/1365), 48.6%(372/765), 45.8%(802/1752) and 25.2%(442/1752), respectively; the results in extra-pulmonary TB cases were 3.4%(6/178), 5.8%(4/69), 35.4%(179/505), and 11.3%(57/505), respectively; the results in old pulmonary TB cases were 0%(0/64), 0%(0/30), 32.2%(28/87), and 9.2%(8/87), respectively; and the results in non-TB cases were 0%(0/121), 0%(0/56), 21.5%(44/205), and 2.4%(5/205), respectively. Of 624 smear-positive and/or

关键词： tuberculosis tuberculosis antibody test Smear Culture

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Luciferase reporter phage phAE85 for rapid detection of rifampicin resistance in clinical isolates of Mycobacterium tuberculosis

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Asian Pacific Journal of Tropical Medicine 2013年第9期6卷 728-731页

作者： Gomathi Sivaramakrishnan Balaji Subramanyam Ponnuraja C Vanaja Kumar department of Bacteriology National Institute for Research in Tuberculosis(Formerly Tuberculosis Research Centre)

Objective:To evaluate luciferase reporter phage(LRP)phAE85 in rapid detection of rifampicin resistance in a region where TB is ***:One hundred and ninety primary isolates on Lowenstein-Jensen medium were *** 7H9 complete medium with and without rifampicin at 2μg/mL was inoculated with standard inoculum from suspensions of the clinical *** incubation for 72 h,LRP was *** 4 h of further incubation,light output from both control and test was measured as relative light *** exhibiting a reduction of less than 50%relative light units in the drug containing vial compared to control were classified as *** were compared with the conventional minimum inhibitory concentration method(MIC)of drug susceptibility ***:The two methods showed high level of agreement of 97%(CI 0.94,0.99)and P value was 0.000 *** sensitivity and specificity of LRP assay for detection of rifampicin resistance were 91%h(CI 0.75,0.98)and 99ct(CI0.95,1.00)*** to detection of resistance by LRP assay was 3 d in comparison with 28 d by the minimum inhibitory concentration ***:LRP assay with phAE85 is 99%specific,91%sensitive and is highly *** the assay offers a simple procedure for drug sensitivity testing,within die scope of semi-automation.

关键词： Mycobacterium tuberculosis Rifampicin resistance Rapid detection

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Effects on type 2 diabetes complicated with pulmonary tuberculosis:regiment of insulin,isoniazid,rifampicin,pyrazinamide and ethambutol versus the regiment plus Qi-boosting and Yin-nourishing decoction of Traditional Chinese Medicine

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Journal of Traditional Chinese Medicine 2015年第3期35卷 260-265页

作者： Shi Guangcan Zhang Ligong department of tuberculosis the First Affiliated Hospital of Xinxiang Medical University

OBjECTIVE:To observe the clinical effect on type 2diabetes mellitus(T2DM) complicated with pulmonary tuberculosis(TB) of insulin,isoniazid,rifampicin,pyrazinamide and ethambutol(conventional medication) administered together with Qi-boosting and Yin-nourishing decoction derived from Traditional Chinese Medicine(TCM).METHODS:A total of 60 patients with T2 DM complicated with pulmonary TB were randomly and equally divided into positive control group and treatment *** control group was treated with Western conventional regiment(WCR):insulin,isoniazid,rifampicin,pyrazinamide,and ethambutol,whereas the treatment group was given both WCR and Qi-boosting and V/n-nourishing decoction prepared from ***:After the treatment,20(66.7%) and 11(36.7%) cases showed sputum bacteria negative conversion in the WCR plus TCM group and WCM group respectively(P < 0.05).A total of 25(83.3%)and 18(60%) cases showed improvement in lung lesion in the WCR plus TCM group and WCM group respectively(P < 0.05).Compared with WCR group,fasting plasma glucose and 2-hour postprandial blood glucose levels in the WCR plus TCM group significantly decreased(P < 0.05 and P < 0.01,respectively).CONCLUSION:Qi-boosting and Yin-nourishing decoction combined with the Western medication showed better curative effect in treating T2 DM complicated with pulmonary TB compared with the group using the conventional Western Medicine alone.

关键词： Diabetes mellitus type 2 tuberculosis pulmonary Qi and Yin deficiency Medicine Chinese traditional

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CLINICAL STUDIES ON ACUPUNCTURE ANESTHESIA IN LUNG RESECTION

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中华医学杂志(英文版) 1980年第5期93卷 287-292页

作者： [Anonymous] department of Acupuncture Anesthesia Beijing Tuberculosis Research Institute

Acupuncture anesthesia(AA)in 1,048 casesof lung resection with a 97.2% success rate isanalyzed for factors affecting efficiency of AA,such as acupuncture point selection and indi-vidual variations in mental activity type,atti-tude towards acupuncture,emotional state,sen-sitivity to acupuncture,tolerance of needling,physiologic parameters and ability to ***,age and occupation do notaffect efficacy.

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Immune Responses and Protective Efficacy Induced by 85B Antigen and Early Secreted Antigenic Target-6 kDa Antigen Fusion Protein Secreted by Recombinant Bacille Calmette-Guérin

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 290-296页

作者： Changhong SHI~1* Xiaowu WANG~1 Hai ZHANG~1 Zhikai XU~2 Yuan LI~2 Lintian YUAN~11 Laboratory Animal Researeh Center,fourth Military Medical University,Xi’an 710033,China2 department of Microbiology,fourth Military Medical University,Xi’an 710033,China Laboratory Animal Research Center Fourth Military Medical UniversityXi'an 710033 China department of Microbiology Fourth Military Medical UniversityXi'an 710033 China

In an attempt to improve immune responses and protective efficacy,we constructed tworecombinant bacille Calmette-Guérin(rBCG)strains expressing an 85B antigen(Ag85B)and early secretedantigenic target-6 kDa antigen(ESAT6)of M vcobacterium tuberculosis(MTB)fusion *** rBCGstrains have the same protein insertion but in a different order(Ag85B-ESAT6 and ESAT6-Ag85B).Thecultured supernatant of rBCG strains and the sera from the mice immunized with the fusion protein Ag85B-ESAT6 or ESAT6-Ag85B formed a band with a fraction size of 37 kDa,equalivalent to the sum of Ag85B *** weeks after BALB/c mice were immunized with BCG or rBCG,spleen lymphocytes showedsignificant proliferation in response to culture filtrate protein of *** with the BCG group,micevaccinated with rBCG elicited a high level increase of immunoglobulin G antibodies to culture filtrate proteinin the *** γ-interferon levels in the lymphocyte culture medium supernatants increased remarkably inthe rBCG1 group,significantly higher than that of the BCG immunized group(P0.05).

关键词： Mycobacterium tuberculosis vaccine Ag85B ESAT6 BCG

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The Impact of Directly Observed Therapy on Preventive Treatment for Latent tuberculosis Infection among Students in Dalian, China

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Biomedical and Environmental Sciences 2015年第8期28卷 611-615页

作者： CHEN Qi WANG Xue Mei QI Yi LIU Xiao Fang JIANG Li Ping HOU Wen ZHOU Ling LU Xi Wei department of Public Health Dalian Medical University department of tuberculosis Prevention and Control Tuberculosis Hospital of Dalian College of Mathematics Liaoning Normal University

Preventive treatment has an essential effect on latent tuberculosis infection(LTBI)[purified protein derivative(PPD)induration≥15 mm].Between2010 and 2013,there were 6 tuberculosis(TB)outbreaks in the universities in... 详细信息

关键词： TB The Impact of Directly Observed Therapy on Preventive Treatment for Latent tuberculosis Infection among Students in Dalian China

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Efficacy and Safety of Combined Bedaquiline and Delamanid Use among Patients with Multidrug-Resistant tuberculosis in Beijing,China

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Biomedical and Environmental Sciences 2024年第10期37卷 1195-1203页

作者： Can Guo Lihui Nie Yanhua Song Rongmei Liu Xiaoguang Wu Yuanyuan Shang Xuxia Zhang Yu Pang Mengqiu Gao department of tuberculosis Beijing Chest HospitalCapital Medical University/Beijing Tuberculosis and Thoracic Tumor Research InstituteBeijing 101149China department of Bacteriology and Immunology Beijing Chest HospitalCapital Medical University/Beijing Tuberculosis and Thoracic Tumor Research InstituteBeijing 101149China

Objectives The combined use of bedaquiline and delamanid(BDQ-DLM)is limited by an increased risk of prolonging the QTc *** retrospectively evaluated patients who received DLM/BDQcontaining regimens at a TB-specialized *** aimed to present clinical efficacy and safety data for Chinese *** This case-control study included patients with multidrug-resistant tuberculosis(MDR-TB)treated with BDQ alone or BDQ plus *** A total of 96 patients were included in this analysis:64 in the BDQ group and 32 in the BDQ+DLM *** the 96 patients with positive sputum culture at the initiation of BDQ alone or BDQ combined with DLM,46 patients(71.9%)in the BDQ group and 29(90.6%)in the BDQ-DLM group achieved sputum culture conversion during *** rate of sputum culture conversion did not differ between the two *** time to sputum culture conversion was significantly shorter in the BDQ-DLM group than in the BDQ *** most frequent adverse event was QTc interval prolongation;however,the frequency of adverse events did not differ between the *** In conclusion,our results demonstrate that the combined use of BDQ and DLM is efficacious and tolerable in Chinese patients infected with *** in the BDQ-DLM group achieved sputum culture conversion sooner than those in the BDQ group.

关键词： Multidrug resistant tuberculosis Bedaquiline Delamanid Efficacy Safety

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Mesenchymal-epithelial Transition Factor Regulates Monocyte Function during Mycobacterial Infection via Indoleamine 2,3-dioxygenase

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Current Medical Science 2022年第2期42卷 407-416页

作者： Bing-fen YANG Fei ZHAI Hong-juan AN Jing JIANG Zhi-hong CAO Yan-hua LIU Jin-wen SU Ruo WANG Xiao-xing CHENG tuberculosis Prevention and Control Key Laboratory Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and TreatmentSenior Department of Tuberculosisthe Eighth Medical Center of Chinese PLA General HospitalBeijing100091China

Objective Mycobacterium tuberculosis(Mtb),the causative agent of tuberculosis(TB),causes an estimated 1.6 million human deaths annually,but the pathogenesis of TB remains *** plays a critical role in the onset and outcome of *** study aimed to uncover the roles of innate and adaptive immunity in *** The gene expression profiles generated by RNA sequencing from human peripheral blood mononuclear cells(PBMCs)stimulated with or without Mtb strain H37Rv antigens were analyzed.A total of 973 differentially expressed mRNAs were *** The differentially expressed genes were enriched in innate immunity signaling *** mesenchymal-epithelial transition factor(MET)gene was significantly upregulated in CD14^(+)monocytes.A MET inhibitor improved the uptake of the BCG strain by monocytes and macrophages as well as inhibited the expression of indoleamine 2,3-dioxygenase(IDO).The expression of IDO was increased in PBMCs stimulated with Mtb antigens,and the IDO inhibitor promoted the expression of CD40,CD83,and *** Our results might provide clues regarding the immunomodulatory mechanisms used by Mtb to evade the host defense system.

关键词： monocytes mycobacteria mesenchymal-epithelial transition factor indoleamine 2,3-dioxygenase

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Efficacy and Safety of Bufei Jiedu Granules in Treating Multidrug-Resistant Pulmonary tuberculosis:A Multi-center,Double-Blinded and Randomized Controlled Trial

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Chinese Journal of Integrative Medicine 2024年第7期30卷 579-587页

作者： ZHANG Shao-yan QIU Lei ZHANG Shun-xian XIAO He-ping CHU Nai-hui ZHANG Xia ZHANG Hui-qiang ZHENG Pei-yong ZHANG Hui-yong LU Zhen-hui Institute of Respiratory Diseases Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai200032China Clinical Research Center Shanghai University of Traditional Chinese MedicineShanghai200032China department of tuberculosis Shanghai Pulmonary HospitalTongji UniversityShanghai200433China department of tuberculosis Beijing Chest HospitalCapital Medical UniversityBeijing101100China department of tuberculosis the Second Hospital of NanjingNanjing210003China department of tuberculosis the First Hospital Affiliated to Xinxiang Medical CollegeXinxiangHenan Province453100China

Objective: To assess the efficacy and safety of Bufei Jiedu(BFJD) Granules as adjuvant therapy for patients with multidrug-resistant pulmonary tuberculosis(MDR-PTB). Methods: A large-scale, multi-center, double-blinded, and randomized controlled trial was conducted in 18 sentinel hospitals in China from December 2012 to December 2016. A total of 312 MDR-PTB patients were randomly assigned to BFJD Granules or placebo groups(1:1) using a stratified randomization method, which both received the long-course chemotherapy regimen for 18 months(6 Am-Lfx-P-Z-Pto, 12 Lfx-P-Z-Pto). Meanwhile, patients in both groups also received BFJD Granules or placebo twice a day for a total of 18 months, respectively. The primary outcome was cure rate. The secondary outcomes included time to sputum-culture conversion, changes in lung cavities and quality of life(QoL) of patients. Adverse reactions were monitored during and after the trial. Results: A total of 216 cases completed the trial, 111 in the BFJD Granules group and 105 in the placebo group. BFJD Granules, as adjuvant treatment, increased the cure rate by 13.6% at the end of treatment, compared with the placebo(58.4% vs. 44.8%, P=0.02), and accelerated the median time to sputum-culture conversion(5 months vs. 11 months). The cavity closure rate of the BFJD Granules group(50.6%, 43/85) was higher than that of the placebo group(32.1%, 26/81;P=0.02) in patients who completed the treatment. At the end of the intensive treatment, according to the 36-item Short Form, the BFJD Granules significantly improved physical functioning, general health, and vitality of patients relative to the placebo group(all P<0.01). Overall, the death rates in the two groups were not significantly different;5.1%(8/156) in the BFJD Granules group and 2.6%(4/156) in the placebo group. Conclusions: Supplementing BFJD Granules with the long-course chemotherapy regimen significantly increased the cure rate and cavity closure rate, and rapidly improved QoL of pati

关键词： alternative medicine Chinese herbal formula Bufei Jiedu Granules multidrug-resistant tuberculosis long-course chemotherapy regimen randomized controlled trial

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