检索结果-南通市图书馆

Function identification of miR159a,a positive regulator during poplar resistance to drought stress

Horticulture Research 2023年第12期10卷 120-136页

作者： Tiantian Fu Chun Wang Yuzhang Yang Xiaoqian Yang Jing Wang Lichun Zhang Zeqi Wang Yanwei Wang State Key Laboratory of tree Genetics and breeding National Engineering Research Center of Tree Breeding and Ecological RestorationKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental PlantsMinistry of EducationThe Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland AdministrationCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China

Drought seriously affects the growth and development of plants.MiR159 is a highly conserved and abundant microRNA family that plays a crucial role in plant growth and stress responses.However,studies of its function in woody plants are still lacking.Here,the expression of miR159a was significantly upregulated after drought treatment in poplar,and the overexpression of miR159a(OX159a)significantly reduced the open area of the stomata and improved water-use efficiency in poplar.After drought treatment,OX159a lines had better scavenging ability of reactive oxygen species and damage of the membrane system was less than that in wild-type lines.MYB was the target gene of miR159a,as verified by psRNATarget prediction,RT-qPCR,degradome sequencing,and 5′rapid amplification of cDNA ends(5′RACE).Additionally,miR159a-short tandem target mimic suppression(STTM)poplar lines showed increased sensitivity to drought stress.Transcriptomic analysis comparing OX159a lines with wild-type lines revealed upregulation of a series of genes related to response to water deprivation and metabolite synthesis.Moreover,drought-responsive miR172d and miR398 were significantly upregulated and downregulated respectively in OX159a lines.This investigation demonstrated that miR159a played a key role in the tolerance of poplar to drought by reducing stomata open area,increasing the number and total area of xylem vessels,and enhancing water-use efficiency,and provided new insights into the role of plant miR159a and crucial candidate genes for the molecular breeding of trees with tolerance to drought stress.

关键词： poplar breeding drought

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transcriptomic and proteomic analyses of far-red light effects in inducing shoot elongation in the presence or absence of paclobutrazol in Chinese pine

引用

Journal of forestry Research 2022年第3期33卷 1033-1043页

作者： Yingtian Guo Shihui Niu Yousry A.El-Kassaby Wei Li National Engineering Laboratory for forest tree breeding Beijing Advanced Innovation Center for Tree Breeding by Molecular DesignCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing100083People’s Republic of China Department of forest and Conservation Sciences Faculty of ForestryUniversity of British Columbia2424 Main MallVancouverBCV6T 1Z4Canada

In angiosperms, low red (R)/far-red (FR) ratio light increases gibberellin (GA) levels. GA signaling in conifer seedlings requires FR to promote shoot elongation and reduce the inhibition of shoot elongation induced by paclobutrazol (PAC), yet the effects of far-red light in inducing shoot elongation in the presence or absence of PAC is poorly understood. In this study, transcriptomic and proteomic analyses was used to examine the molecular mechanism of FR regulation of shoot elongation in Chinese pine (Pinus tabuliformis Carr.) seedlings in the presence of PAC. Four treatments were compared: white light + water (WW), FR + water (RW), white light + PAC (WP), and FR + PAC (RP), and 1436 differentially expressed genes (DEGs) and 450 differentially expressed proteins (DEPs) were identified in RW_WW (RW contrast WW), and 1862 DEGs and 481 DEPs in RP_WP (RP contrast WP). Metabolic and signal transduction pathway analyses of DEGs and DEPs in RW_WW and RP_WP, indicated that the former required more energy than the latter. Moreover, gibberellic acid, auxin, and brassinolide are equally important in RW_WW and RP_WP for shoot elongation, except for the ethylene pathway. Amino acid metabolism and cell wall organization were significantly enriched in RW_WW and RP_WP, respectively. In summary, RW_WW and RP_WP had different effects in secondary metabolism, energy metabolism, amino acid metabolism, cell wall organization, and hormone response. These results provide an important theoretical and reference basis for studying the regulatory effect of low R/FR and PAC in conifer shoot elongation.

关键词： Low R/FR ratio Pinus tabuliformis Paclobutrazol Proteome Shoot elongation Transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Differences in leaf cuticular wax induced by whole-genome duplication in autotetraploid sour jujube

引用

Horticultural Plant Journal 2024年第1期10卷 66-76页

作者： Meng Li Chenxing Zhang Lu Hou Xinru Liu Hanqing Zhao Xiaoming Pang Wenhao Bo Yingyue Li National Engineering Research center of tree breeding and Ecological Restoration College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China Key Laboratory of Genetics and breeding in forest trees and Ornamental Plants Ministry of EducationCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China The tree and Ornamental Plant breeding and Biotechnology Laboratory of National forestry and Grassland Administration Beijing Forestry UniversityBeijing 100083China

Drought-resistant plants exhibit strong water retention capability.In this regard,the autotetraploid sour jujube leaves exhibit better water retention than diploid leaves.Morphological comparisons and physiological comparisons of diploid and autotetraploid leaves showed that the autotetraploid leaves had thicker leaf cuticles and more leaf wax accumulation than the diploid leaves,which could reduce cuticle permeability and improve the drought tolerance of leaves.In this study,the cuticular wax crystalloids on the adaxial and abaxial sides of young and mature jujube leaves were observed in the two ploidy types,and unique cuticular wax crystalloids covering a large area of the cuticle on autotetraploid sour jujube leaves may provide an advantage in reducing leaf non-stomata transpiration and improving plant drought tolerance.Based on the transcriptome,115 differentially expressed genes between diploids and autotetraploids were further analyzed and found to be involved in the accumulation of cuticular wax components,including terpenoids,fatty acids,and lipids,as well as ABC transporter and wax biosynthetic process.Finally,14 genes differentially expressed between glossy autotetraploid leaves and nonglossy diploid leaves,such as LOC107414787,LOC107411574 and LOC107413721,were screened as candidate genes by qRT-PCR analysis.This findings provided insights into how polyploidization improved drought tolerance.

关键词： Autotetraploid Jujube Drought tolerance Cuticular wax RNA-seq

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genomic clines across the species boundary between a hybrid pine and its progenitor in the eastern Tibetan Plateau

引用

Plant Communications 2023年第4期4卷 240-254页

作者： Jing-Fang Guo Wei Zhao Bea Andersson Jian-Feng Mao Xiao-Ru Wang National Engineering Research center of tree breeding and Ecological Restoration State Key Laboratory of Tree Genetics and BreedingKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental PlantsMinistry of EducationCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China Department of Ecology and Environmental Science UmeåPlant Science CentreUmeåUniversity90187 UmeåSweden

Most species have clearly defined distribution ranges and ecological niches.The genetic and ecological causes of species differentiation and the mechanisms that maintain species boundaries between newly evolved taxa and their progenitors are,however,less clearly defined.This study investigated the genetic structure and clines in Pinus densata,a pine of hybrid origin on the southeastern Tibetan Plateau,to gain an understanding of the contemporary dynamics of species barriers.We analyzed genetic diversity in a range-wide collection of P.densata and representative populations of its progenitors,Pinus tabuliformis and Pinus yunnanensis,using exome capture sequencing.We detected four distinct genetic groups within P.densata that reflect its migration history and major gene-flow barriers across the landscape.The demographies of these genetic groups in the Pleistocene were associated with regional glaciation histories.Interestingly,population sizes rebounded rapidly during interglacial periods,suggesting persistence and resilience of the species during the Quaternary ice age.In the contact zone between P.densata and P.yunnanensis,3.36%of the analyzed loci(57849)showed exceptional patterns of introgression,suggesting their potential roles in either adaptive introgression or reproductive isolation.These outliers showed strong clines along critical climate gradients and enrichment in a number of biological processes relevant to high-altitude adaptation.This indicates that ecological selection played an important role in generating genomic heterogeneity and a genetic barrier across a zone of species transition.Our study highlights the forces that operate to maintain species boundaries and promote speciation in the Qinghai-Tibetan Plateau and other mountain systems.

关键词： ecological selection demographic history genomic cline introgression outliers reproductive isolation species boundary

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Designing Microarray and RNA-Seq Experiments for Greater Systems Biology Discovery in Modern Plant Genomics

引用

Molecular Plant 2015年第2期8卷 196-206页

作者： Chuanping Yang Hairong Wei State Key Laboratory of forest tree Genetics and breeding Northeast Forestry University Harbin Heilongjiang 150040 China Biotechnology Research center School of Forest Resources and Environmental Science Michigan Technological University Houghton MI 49931 USA

Microarray and RNA-seq experiments have become an important part of modern genomics and systems biology. Obtaining meaningful biological data from these experiments is an arduous task that demands close attention to many details. Negligence at any step can lead to gene expression data containing inadequate or composite information that is recalcitrant for pattern extraction. Therefore, it is imperative to carefully consider experimental design before launching a time-consuming and costly experiment. Contemporarily, most genomics experiments have two objectives：（1） to generate two or more groups of comparable data for identifying differentially expressed genes, gene families, biological processes, or metabolic pathways under experimental conditions; （2） to build local gene regulatory networks and identify hierarchically important regulators governing biological processes and pathways of interest. Since the first objective aims to identify the active molecular identities and the second provides a basis for understanding the underlying molecular mechanisms through inferring causality relationships mediated by treatment, an optimal experiment is to produce biologically relevant and extractable data to meet both objectives without substantially increasing the cost. This review discusses the major issues that researchers commonly face when embarking on microarray or RNA-seq experiments and summarizes important aspects of experimental design, which aim to help researchers deliberate how to generate gene expression profiles with low background noise but with more interaction to facilitate novel biological discoveries in modern plant genomics.

关键词： experimental design microarray RNA-seq treatment time course gene regulatory network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Site index for Chinese fir plantations varies with climatic and soil factors in southern China

引用

Journal of forestry Research 2022年第6期33卷 1765-1780页

作者： Xiaoyan Li Aiguo Duan Jianguo Zhang State Key Laboratory of tree Genetics and breeding Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Collaborative Innovation center of Sustainable forestry in Southern China Nanjing Forestry UniversityNanjing 210037People’s Republic of China

Chinese fir[Cunninghamia lanceolata(Lamb.)Hook.]has a large native distribution range in southern China.Here,we tested differences in productivity of Chinese fir plantations in different climatic regions and screened the main environmental factors affecting site productivity in each region.Relationships of a Chinese fir site index with climatic factors and the soil physiochemical properties of five soil layers were examined in a long-term positioning observation trial comprising a total of 45 permanent plots in Fujian(eastern region in the middle subtropics),Guangxi(south subtropics)and Sichuan(central region in the middle subtropics)in southern China.Linear mixed effects models were developed to predict the site index for Chinese fir,which was found to vary significantly among different climatic regions.Available P,total N,bulk density and total K were dominant predictors of site index in three climatic regions.The regional linear mixed models built using these predictors in the three climatic regions fit well(R~2=0.86–0.97).For the whole study area,the available P in the 0–20-cm soil layer and total N in the 80–100-cm soil layer were the most indicative soil factors.MAP was the most important climatic variable influencing the site index.The model evaluation results showed that the fitting performance and prediction accuracy of the global site index model using the climatic region as the dummy variable and random parameters and the most important soil factors of the three climatic regions as predictors was higher than that of global site index model using the climatic variable and the most indicative soil variables of the whole study area.Our results will help with further evaluation of site quality of Chinese fir plantations and the selection of its appropriate sites in southern China as the climatic changes.

关键词： Site productivity Site index Climate Soil Chinese fir

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genotype Screening of Recipient Resources with High Regeneration and Transformation Efficiency in Chrysanthemum

引用

Phyton-International Journal of Experimental Botany 2022年第4期91卷 869-888页

作者： Yajun Li Yumeng Cui Bingjie Wang Yue Li Mengmeng Zhang Silan Dai He Huang Beijing Advanced Innovation center for tree breeding by Molecular Design Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of EducationMinistrySchool of Landscape ArchitectureBeijing Forestry UniversityBeijing100083China Beijing Botanical Garden Beijing100093China

Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum,which relies on an optimal regeneration and transformation system.However,the regeneration system of different chrysanthemum cultivars varies,and the regeneration time of most cultivars is long.To screen cultivars with highly efficient regeneration,leaves and shoot tip thin cell layers(tTCL)from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media(MS)supplemented with 1.0-5.0 mg L^(−1)6-benzylaminopurine(6-BA)and 0.1-1.0 mg L^(−1)α-naphthaleneacetic(NAA).The results showed that the most efficient regeneration media were MS+6-BA 1.0 mg L^(−1)+NAA 0.5 mg L^(−1)for leaf explants and MS+6-BA 5.0 mg L^(−1)+NAA 0.1 mg L^(−1)for tTCL explants.Subsequently,another 13 chrysanthemum cultivars were screened by using the media,and finally,three cultivars with high regeneration efficiency were obtained from 21 cultivars.Among these,C1 had the highest regeneration efficiency:the regeneration rate of leaf explants reached 80.0%after 42 days of culture,and the regeneration rate of tTCL explants reached 100%after 31 days of culture.Furthermore,we also established the transformation system for C1 as follows:preculturing for one day,infecting with Agrobacterium suspension(OD_(600)=0.6)for 10 min,and cultivating in the regeneration medium with 350 mg L^(−1)carbenicillin and 10 mg L^(−1)kanamycin,thus ultimately achieving a transformation rate of 4.0%.In this study,a new chrysanthemum cultivar with an efficient regeneration and transformation system was screened,which is beneficial to enrich the flower color of chrysanthemum transgenic plant recipients and to the functional research of flower color or type-related genes.

关键词： Chrysanthemum tissue culture regeneration system genetic transformation cultivars screening

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Growth response of Pterocarpus santalinus seedlings to native microbial symbionts(arbuscular mycorrhizal fungi and Rhizobium aegyptiacum)under nursery conditions

引用

Journal of forestry Research 2021年第1期32卷 225-231页

作者： Arumugam Karthikeyan Thangavel Arunprasad Institute of forest Genetics and tree breeding Coimbatore 641002India

The objective of this research was to improve the growth and biomass of Pterocarpus santalinus L.f.(an endangered leguminous tree)using native microbial symbionts such as arbuscular mycorrhizal fungi and Rhizobium associated with native populations of P.santalinus.The native arbuscular mycorrhizal fungi isolated from P.santalinus soils were identifi ed as(1)Glomus fasciculatum;(2)Glomus geosporum;and Glomus aggregatum.A nitrogenfi xing microbial symbiont was isolated from the root nodules of P.santalinus and identifi ed as Rhizobium aegyptiacum by 16s rRNA gene sequencing.These microbial symbionts were inoculated individually and in combination into P.santalinus seedling roots.After 90 days,growth and biomass had improved compared with uninoculated controls.Shoot and root lengths,number of leaves,stem circumference,number of root nodules,biomass,nutrient uptake and seedling quality index were signifi cantly increased by a combined inoculation of arbuscular mycorrhizal fungi+Rhizobium aegyptiacum.It was concluded that native microbial symbionts positively infl uenced P.santalinus seedling growth which will be helpful for successful fi eld establishment.

关键词： Arbuscular mycorrhizal fungi Microbial symbionts Pterocarpus santalinus Red sanders Rhizobium aegyptiacum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Early evaluation of growth traits of Larix kaempferi clones

引用

Journal of forestry Research 2018年第4期29卷 1024-1032页

作者： Yanyan Pan Shuchun Li Chenglu Wang Wenjun Ma Guiyou Xu Lili Shao Kailong Li Xiyang Zhao Tingbo Jiang State Key Laboratory of tree Genetics and breeding School of Forestry Northeast Forestry University Harbin 150040 People's Republic of China forestry Department of Jilin Province Tree Seedling Management Station Changchun 130000 People's Republic of China Wudaogou forestry center of Liuhe County Tonghua 135300 People's Republic of China State Key Laboratory of forest Genetics and tree breeding Key Laboratory of tree breeding and Cultivation of State forestry Administration Research Institute of forestry Chinese Academy of Forestry Beijing Beijing 100091 People's Republic of China

Early selection is an important method to shorten the breeding cycle for tree species, which may differ in the time for early selection. To evaluate the early selected time for Larix kaempferi, tree height and diameter at breast height of 57 L. kaempferi clones were measured over many different growth years. The results indicated that, except for age × clone interaction for diameter at breast height （P = 0.741）, there were significant differences among all variation sources （P 〈 0.01）. The coefficient of phenotypic variation ranged from 14.89 to 35.65% for height and from 19.17 to 23.86% for diameter at breast height in different growth years. The repeatability of height and of diameter at breast height among clones was high, ranging from 0.6181 to 0.8531 （height） and from 0.8443 to 0.8497 （diameter at breast height）, in different growth years. There were significant positive correlations between all pairs of growth traits except between height in the 2nd growth year and height in the 30th growth year; and between height in the 2nd growth year × diameter at breast height in the 30th growth year. With a comprehen- sive evaluation method and a selection ratio of 10%, L65, L1, L62, L9, L15, and L78 were selected as excellent clones in the 30th growth year. Their average values of height and diameter at breast height were 9.81 and 16.57% higher than the overall average, representing genetic gains of 6.46 and 13.99%, respectively. This study provides a theoretical foundation for the genetic improvement of L. kaempferi.

关键词： Larix kaempferi Genetic Variation.Comprehensive evaluation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development and application of microsatellite markers within transcription factors in flare tree peony(Paeonia rockii)based on next-generation and single-molecule long-read RNA-seq

引用

Journal of Integrative Agriculture 2021年第7期20卷 1832-1848页

作者： LIU Na CHENG Fang-yun GUO Xin ZHONG Yuan Beijing Advanced Innovation center for tree breeding by Molecular Design/Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular breeding/National Engineering Research center for Floriculture/Key Laboratory of Genetics and breeding in forest trees and Ornamental Plants of Ministry of Education/Peony International Institute School of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083P.R.China

tree peonies native to China are a precious crop with ornamental,medicinal and edible oil properties,of which flare tree peony(Paeonia rockii)is one of the most significant germplasms in Paeonia.The development and application of expressed sequence tag-simple sequence repeat(EST-SSR)markers are very valuable for genetic and breeding applications,but EST-SSR resources for the genus Paeonia are still limited.In this study,we first reported the development of SSRs within transcription factors(TFs)in P.rockii based on next-generation sequencing(NGS)and single-molecule long-read sequencing(SMLRS).A total of 166 EST-SSRs containing six nucleotide repeat types were identified from 959 candidate TFs associated with yield,with an average of one SSR per 5.83 unigenes.In total,102(61.45%)pairs of primers produced amplification products in the two RNA-seq cultivars.Among them,58(56.86%)pairs of primers from 18 gene families(AP2,b HLH,HSF,etc.)were identified to be polymorphic both in the parents of a linkage mapping population and in eight randomly selected accessions of P.rockii.Further,the 58 EST-SSRs indicated a high level of informativeness with PIC values ranging from 0.32 to 0.91(mean 0.70)after assessment in 37 tree peony accessions.Transferability studies indicated that the amplification ratio of the 58 pairs of primers ranged from 89.66 to 100%across seven species of Paeonia.In addition,a genetic relationship study was performed in 62 accessions.Cluster analysis using the neighbour-joining(NJ)tree demonstrated that major clusters corresponded to the known pedigree trees.Taken together,these newly developed EST-SSRs have a potential use in the conservation of tree peony germplasm and marker-assisted selection(MAS)breeding.

关键词： flare tree peony(Paeonia rockii) EST-SSR markers polymorphism genetic diversity transferability genetic relationships

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：