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PbrARF4 contributes to calyx shedding of fruitlets in ‘Dangshan Suli’ pear by partly regulating the expression of abscission genes

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Horticultural Plant Journal 2024年第2期10卷 341-354页

作者： Guoling Guo Pengfei Wei Tao Yu Haiyan Zhang Wei Heng Lun Liu Liwu Zhu Bing Jia school of Horticulture Anhui Agricultural UniversityHefeiAnhui 230036China Huayuan foreign language school Zhejiang Normal UniversityJinhuaZhejiang 322100China Agricultural Experimental Center of Guiyang Guiyang Agriculture and Rural BureauGuiyangGuizhou 550018China

Fruitlet calyx shedding in pear plants is apparently regulated via numerous pathways that involve both environmental triggers and phytohormones cues such as auxin. In this study, we found at 10 days after full bloom (DAFB) higher levels of indoleacetic acid (IAA) and tryptophan (Trp) in calyx persistence fruitlet (CPF) than calyx shedding fruitlet (CSF) ofDanshan Suli’ pear (Pyrus bretschneideri Rhed.). Consisting with this, the activity of indolealdehyde oxidase (IAAIdO), which promotes IAA synthesis, was remarkably increased, and that of peroxidase(POD), which degrades IAA, dropped markedly in CPF but not in CSF. Further, qRT-PCR results revealed that most of 31 PbrARFs (encoding auxin response factors) in Pyrus bretschneideri were highly expressed in CPF, whereas PbrARF4, PbrARF24 and PbrARF26 were significantly downregulated in CPF vis-a-vis CSF. Phylogenetic analysis revealed that 6 PbrARFs clustered in the group III, where PbrARF4 showed the closest affinity with AtARF1 that promotes organ abscission, indicating a putative role of PbrARF4 in mediating the process of calyx shedding in pear. In fact, the ectopic overexpression of PbrARF4 in Solanum lycopersicum resulted in an earlier-formed and deeper abscission layer (AL) in the transgenic plants, whose calyxes were more prone to wilt at the mature red stage (MR) compared with the control plants (wild-type). More importantly, expression levels of the abscission genes SILS and Sl Cel2 in transgenic plants overexpressing PbrARF4 were significantly upregulated in comparation with the WT, whereas those of Sl BI and Sl TAPG2 were considerably inhibited. Further, PbrJOINTLESS and PbrIDA,the two genes related to calyx shedding in pear, were up-regulated more in CSF than CPF. The findings contribute to a better understanding of PbrARFs involved in fruitlet calyx shedding of pear, which could prove beneficial to improving the quality of pear fruit.

关键词： Pear Pyrus bretschneideri Rehd Calyx shedding IAA PbrARF4 Abscission genes

Artificial Immune Detection for Network Intrusion Data Based on Quantitative Matching Method

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Computers, Materials & Continua 2024年第2期78卷 2361-2389页

作者： CaiMing Liu Yan Zhang Zhihui Hu Chunming Xie school of Electronic Information and Artificial Intelligence Leshan Normal UniversityLeshan614000China Intelligent Network Security Detection and Evaluation Laboratory Leshan Normal UniversityLeshan614000China Internet Natural language Intelligent Processing Key Laboratory of Education Department of sichuan Province Leshan Normal UniversityLeshan614000China

Artificial immune detection can be used to detect network intrusions in an adaptive approach and proper matching methods can improve the accuracy of immune detection *** paper proposes an artificial immune detection model for network intrusion data based on a quantitative matching *** proposed model defines the detection process by using network data and decimal values to express features and artificial immune mechanisms are simulated to define immune ***,to improve the accuracy of similarity calculation,a quantitative matching method is *** model uses mathematical methods to train and evolve immune elements,increasing the diversity of immune recognition and allowing for the successful detection of unknown *** proposed model’s objective is to accurately identify known intrusions and expand the identification of unknown intrusions through signature detection and immune detection,overcoming the disadvantages of traditional *** experiment results show that the proposed model can detect intrusions *** has a detection rate of more than 99.6%on average and a false alarm rate of 0.0264%.It outperforms existing immune intrusion detection methods in terms of comprehensive detection performance.

关键词： Immune detection network intrusion network data signature detection quantitative matching method

Deep Broad Learning for Emotion Classification in Textual Conversations

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Tsinghua Science and Technology 2024年第2期29卷 481-491页

作者： Sancheng Peng Rong Zeng Hongzhan Liu Lihong Cao Guojun Wang Jianguo Xie Center for Linguistics and Applied Linguistics and Laboratory of Language Engineering and ComputingGuangdong University of Foreign StudiesGuangzhou 510006China Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices South China Normal UniversityGuangzhou 510006China school of Computer Science and Cyber Engineering Guangzhou UniversityGuangzhou 510006China Modern Education Technology Center Guangdong University of Foreign StudiesGuangzhou 510006China

Emotion classification in textual conversations focuses on classifying the emotion of each utterance from textual *** is becoming one of the most important tasks for natural language processing in recent ***,it is a challenging task for machines to conduct emotion classification in textual conversations because emotions rely heavily on textual *** address the challenge,we propose a method to classify emotion in textual conversations,by integrating the advantages of deep learning and broad learning,namely *** aims to provide a more effective solution to capture local contextual information(i.e.,utterance-level)in an utterance,as well as global contextual information(i.e.,speaker-level)in a conversation,based on Convolutional Neural Network(CNN),Bidirectional Long Short-Term Memory(Bi-LSTM),and broad *** experiments have been conducted on three public textual conversation datasets,which show that the context in both utterance-level and speaker-level is consistently beneficial to the performance of emotion *** addition,the results show that our proposed method outperforms the baseline methods on most of the testing datasets in weighted-average F1.

关键词： emotion classification textual conversation Convolutional Neural Network(CNN) Bidirectional Long Short-Term Memory(Bi-LSTM) broad learning

Research progress on the identification and intervention strategies of situational crisis of college students

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Journal of Social Science Development Research

Journal of Social Science Development Research 2024年第2期2卷 18-24页

作者： Yong Wang school of International language and Culture Chengdu University of Foreign LanguagesYibinSichuanChina

In order to identify and improve the level of situational crisis of college students,this paper studies and analyzes the identification and intervention strategies of situational crisis of college students,in order to provide references for the realization of early detection and prevention of situational crisis,improvement of intervention ability and formulation of college students'mental health service system.

关键词： Educational Ecology College English Teaching Teaching Team Development

青藏高原典型牧区生态系统服务与人类福祉的时空协调发展研究

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Journal of Geographical Sciences 2024年第2期34卷 252-288页

作者：任思雨景海超钱雪雪刘颖慧 State Key Laboratory of Earth Surface Processes and Resource Ecology Faculty of Geographical ScienceBeijing Normal UniversityBeijing 100875China Shenzhen Nanshan foreign language school Shenzhen 518063GuangdongChina

In this study,the interplay between ecosystem services and human well-being in Seni district,which is a pastoral region of Nagqu city on the Qinghai-Tibet Plateau,is *** the improved InVEST model,CASA model,coupling coordination model,and hierarchical clustering method,we analyze the spatiotemporal patterns of ecosystem services,the levels of resident well-being levels,and the interrelationships between these factors over the period from 2000 to *** findings reveal significant changes in six ecosystem services,with water production decreasing by 7.1%and carbon sequestration and soil conservation services increasing by approximately 6.3%and 14.6%,*** the habitat quality and landscape recreation services remained ***,the towns in the eastern and southern areas exhibited higher water production and soil conservation services,while those in the central area exhibited greater carbon sequestration *** coupling and coordination relationship between ecosystem services and human well-being improved significantly over the study period,evolving from low-level coupling to coordinated *** clustering was used to classify the 12 town-level units into five *** subjective well-being townships had lower livestock breeding services,while high subjective well-being townships had higher supply,regulation,and support ecosystem *** transportation conditions were associated with higher subjective well-being in townships with low supply *** recommend addressing the identified transportation disparities and enhancing key regulatory and livestock breeding services to promote regional sustainability and improve the quality of life for Seni district residents,thus catering to the diverse needs of both herdsmen and citizens.

关键词： ecosystem services human well-being coupling coordination regional differences spatiotemporal analysis

Effect of foot reflexology on an infant with sensorineural hearing loss:A case report

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World Journal of Clinical Cases 2024年第8期12卷 1437-1441页

作者： Yu-Jun Lee Mao-Qing Chen Jie Dong Graduate school Xi'an International Studies UniversityNorth Sichuan Medical UniversityXi'an 710061Shaanxi ProvinceChina Department of Neural Disease North Sichuan Medical UniversityNanchong 635200Sichuan ProvinceChina Department of Nursing North Sichuan Medical UniversityNachong 635200Sichuan ProvinceChina Key Laboratory for Artificial Intelligence and Cognitive Neuroscience of language Xi’an International Studies UniversityXi’an 710128Shaanxi ProvinceChina

BACKGROUND Our study contributes to the further understanding of the mechanism of foot *** reflexology has been reported to affect hearing recovery,but no physiological evidence has been *** lack of evidence hampers the acceptance of the technique in clinical *** SUMMARY A girl was taken to North sichuan Medical university affiliated Hospital for a hearing screen by her *** parents reported that her hearing level was the same as when she was *** girl was diagnosed with sensorineural hearing loss(SNHL)by a doctor in the otolaryngology *** we introduced the foot reflexology project,the parents agreed to participate in the *** 6 months of foot reflexology treatment,the hearing threshold of the girl recovered to a normal level,below 30 *** Foot reflexology should be encouraged in clinical practice and for families of infants with SNHL.

关键词： Infant with sensorineural hearing loss Foot reflexology Functional connectivity language development Case report

Cultivation of Translators for Multimodal Translation in the New Media Age

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Journal of Contemporary Educational Research 2024年第5期8卷 37-45页

作者： Minghui Long school of foreign languages and Literatures Chongqing Normal UniversityChongqing 401331China

In the 21st century,the development of digital and new media technologies has ushered in an age of pervasive multimodal communication,which has significantly amplified the role of multimodal translation in facilitating crosscultural *** the profound impact of these developments,the prevailing translation pedagogy remains predominantly focused on the enhancement of linguistic translation skills,with noticeable neglect of the imperative to cultivate students’competencies in multimodal *** on the distinctive characteristics and challenges that multimodal translation presents in the context of new media,this study delves into the formulation of educational objectives and curriculum design for the training of multimodal *** intent is to propose a framework that can guide the preparation of translators who are adept and equipped to navigate the complexities and demands of the contemporary age.

关键词： Multimodal communication Multimodal translation Multimodal translation competencies Visual literacy Ability to integrate verbal and non-verbal modes Curriculum design

The Application of Analytic Hierarchy Process Method in the Evaluation of Educational Quality in Colleges and Universities

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Journal of Contemporary Educational Research 2024年第7期8卷 335-342页

作者： Junbo Lei Yanxia Xu Junpeng Wang school of foreign languages Heilongjiang University of Business and TechnologyHarbin 150025Heilongjiang ProvinceChina school of foreign language Harbin Cambridge UniversityHarbin 150086Heilongjiang ProvinceChina English Department Heilongjiang International UniversityHarbin 150500Heilongjiang ProvinceChina

Talent quality is the lifeline of colleges and *** on employment guidance,this paper establishes a student quality evaluation and control system including knowledge,moral character,and ability,and uses the Analytic Hierarchy Process(AHP)method to analyze the factors affecting the quality of education in colleges and *** addition,some suggestions are provided to improve the quality of students.

关键词： Student quality Knowledge Ability Literacy

Reform and Development of English Teaching in Colleges and Universities with the Integration of ChatGPT Technology

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Journal of Contemporary Educational Research 2024年第6期8卷 71-76页

作者： Jie Cao Jing Cao Yuan Tao College of foreign Studies Hubei Normal UniversityHuangshi435002Hubei ProvinceChina Boda College of Jilin normal university Siping 136000Jilin ProvinceChina school of Mathematics and Computer Science Jilin Normal UniversitySiping 136000Jilin ProvinceChina

As an artificial intelligence-powered language generation model based on natural language processing,ChatGPT,like other artificial intelligence technologies,possesses super real-time dialogue capabilities and is considered an innovative and intelligent auxiliary teaching tool in the field of language *** article first analyzes the current state of development of artificial intelligence technologies such as ***,it examines the challenges encountered in the reform and development of English teaching in colleges and universities in ***,it summarizes the applications and recommendations for using ChatGPT technology in college-level English teaching.

关键词： ChatGPT Artificial intelligence technologies College English Reform and development