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Free fatty acids receptors 2 and 3 control cell proliferation by regulating cellular glucose uptake

World Journal of Gastrointestinal Oncology 2020年第5期12卷 514-525页

作者： Saeed Al Mahri Amal Al Ghamdi Maaged Akiel Monira Al Aujan Sameer Mohammad Mohammad Azhar Aziz King Abdullah international Medical Research Center Riyadh 11426Saudi Arabia King Saud bin Abdulaziz University for Health Sciences Riyadh 11426Saudi Arabia Ministry of the National Guard-Health Affairs Riyadh 11426Saudi Arabia Department of Experimental Medicine King Abdullah International Medical Research CenterRiyadh 11426Saudi Arabia Colorectal Cancer Research Program King Abdullah International Medical Research CenterRiyadh 11426Saudi Arabia Department of Clinical Laboratory Sciences College of Applied Medical SciencesKing Saud bin Abdulaziz University for Health SciencesRiyadh 11426Saudi Arabia Department of Medical Genomics King Abdullah International Medical Research CenterRiyadh 11426Saudi Arabia Department of Human and Molecular genetics Virginia Commonwealth UniversityRichmondVA 23284United States

BACKGROUND Colorectal cancer(CRC)is a worldwide problem,which has been associated with changes in diet and lifestyle *** a result of colonic fermentation of dietary fibres,short chain free fatty acids are generated which activate free fatty acid receptors(FFAR)2 and ***2 and FFAR3 genes are abundantly expressed in colonic epithelium and play an important role in the metabolic homeostasis of colonic epithelial *** studies point to the involvement of FFAR2 in colorectal *** To understand the role of short chain FFARs in *** Transcriptome analysis console software was used to analyse microarray data from CRC patients and cell *** employed short-hairpin RNA mediated down regulation of FFAR2 and FFAR3 genes,which was validated using quantitative real time polymerase chain *** for glucose uptake and cyclic adenosine monophosphate(cAMP)generation was done along with immunofluorescence studies to study the effects of FFAR2/FFAR3 *** measuring cell proliferation,we employed real time electrical impedancebased assay available from *** Microarray data analysis of CRC patient samples showed a significant down regulation of FFAR2 gene *** prompted us to study the FFAR2 in ***,FFAR3 shares significant structural and functional homology with FFAR2,we knocked down both these receptors in CRC cell line HCT *** modified cell lines exhibited higher proliferation rate and were found to have increased glucose uptake as well as increased level of glucose transporter ***,FFAR2 and FFAR3 signal through G protein subunit(Gαi),knockdown of these receptors was associated with increased *** of protein kinase A(PKA)did not alter the growth and proliferation of these cells indicating a mechanism independent of cAMP/PKA *** Our results suggest role of FFAR2/FFAR3 genes in increased proliferation of colon cancer cells via enhanced glucose uptake and exclude t

关键词： Cell proliferation Glucose transporter 1 Colorectal cancer Free fatty acids receptor 2 Free fatty acids receptor 3 Glucose uptake

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Exploration and Validation of the Potential Downstream Genes Underlying ipa1-2D Locus for Rice Panicle Branching

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Phyton-international Journal of Experimental Botany 2021年第3期90卷 773-787页

作者： Lin Zhang Dong Xie Zhong Bian Yiting Zou Han Zhou Wenlu Cai Hadi Yeilaghi Xiaolei Fan Changquan Zhang Qiaoquan Liu Joint international Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingYangzhou UniversityYangzhou225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Jiangsu Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhou225009China

In recent years,some super hybrid rice varieties were bred with strong culms and large panicles,which are mainly contributed by the ipa1-2D locus.A gain-of-function allele of OsSPL14 is the ipa1-2D and it can greatly increase the panicle primary branch ***,the key downstream genes mediating this trait variation are not fully *** this study,we developed high-quality near-isogenic lines(NILs)with a difference of only 30 kb chromosomal segment covering the ipa1-2D *** the NILs,we explored the impact of ipa1-2D on five sequential stages of early inflorescence development,and found that the locus can greatly enhance the initiation of primary branch meristems.A transcriptomic analysis was performed to unveil the downstream molecular network of ipa1-2D,and 87 genes were found differentially expressed,many of which are involved in metabolism and catalysis *** addition,transgenic lines of overexpression and RNA interference were generated to shape different levels of *** were also used to validate the expression variation explored by *** on the gene annotation,twelve potential downstream targets of ipa1-2D were selected,and their expression variation was confirmed by qRT-PCR analysis both in NILs and transgenic *** research expands the molecular network underlying ipa1-2D and provides novel gene information which might be involved in the control of panicle *** discussed the potential function of identified genes and highlighted their values for future function exploration and breeding application.

关键词： Rice ipa1-2D panicle branching inflorescence meristems gene expression

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Fine Mapping and Candidate Gene Prediction of the Quantitative Trait Locus qPL8 for Panicle Length in Rice

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Phyton-international Journal of Experimental Botany 2021年第3期90卷 789-802页

作者： Lin Zhang Yiting Zou Zhong Bian Dong Xie Hadi Yeilaghi Xiaolei Fan Dongsheng Zhao Qiaoquan Liu Joint international Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education Jiangsu Key Laboratory of Crop Genomics and Molecular BreedingYangzhou UniversityYangzhou225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Jiangsu Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhou225009China

Rice panicle is the sink organ where assimilation product accumulates,and its morphology determines the rice *** length has been suggested as a yield-related trait,but the genetic factor for its control is still *** this study,we carried out fine-mapping of qPL8,a QTL identified for panicle length in our previous *** isogenic line(NIL)with qPL8 exhibited elongated panicle without obvious effect on other panicle *** five key recombinants from NIL population,the locus was finally narrowed down to a 278-kb region,where 44 genes are *** comparing the genomic sequence of two parents,17 genes were identified with SNPs or InDels variations in the coding *** analysis showed that eight genes were up-regulated in the NIL with *** both the coding variation and expression status,several candidate genes for the locus were identified,and OsMADS37 was raised as the most possible ***,an expression QTL(eQTL)also resides in the locus,leading to a cluster of gene expression variation in the *** study will facilitate the application of qPL8 locus in rice breeding for yield potential.

关键词： Rice QTL panicle length fine mapping qPL8

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Long-term effects of mowing on plasticity and allometry of Leymus chinensis in a temperate semi-arid grassland,China

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Journal of Arid Land 2016年第6期8卷 899-909页

作者： LI Xiliang HOU Xiangyang REN Weibo Taogetao BAOYIN LIU Zhiying Warwick BADGERY LI Yaqiong WU Xinhong XU Huimin National forage Improvement Center Key Laboratory of Grassland Resources and Utilization of Ministry of AgricultureInstitute of Grassland ResearchChinese Academy of Agricultural Sciences Ecology College of Life SciencesInner Mongolia University New South Wales Department of Primary Industries Orange Agricultural Institute

Mowing is an important land management practice for natural semi-arid regions. A growing body of empirical evidence shows that different mowing regimes affect the functioning of grassland ecosystems. However, the responses of plant functional traits to long-term mowing and their allometric scaling under long-term mowing are poorly understood. For a better understanding of the effects of mowing on grassland ecosystems, we analyzed the allometric traits of leaves and stems of Leymus chinensis （Trin.） Tzvel., a dominant grass species in eastern Eurasian temperate grassland, at different mowing intensities （no clipping, clipping once every two years, once a year and twice a year）. Experiments were conducted on plots established over a decade ago in a typical steppe of Xilinhot, Inner Mongolia, China. Results showed that most of the functional traits of L. chinensis decreased with the increased mowing intensity. The responses of leaves and stems to long-term mowing were asymmetric, in which leaf traits were more stable than stem traits. Also significant allometric relationships were found among most of the plant functional traits under the four mowing treatments. Sensitive traits of L. chinensis （e.g. leaf length and stem length） were primary indicators associated with aboveground biomass decline under high mowing intensity. In conclusion, the allometric growth of different functional traits of L. chinensis varies with different long-term mowing practices, which is likely to be a strategy used by the plant to adapt to the mowing disturbances.

关键词： Leymus chinensis allometry plant functional traits mowing semi-arid grassland Inner Mongolia

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QTL analysis of rice low temperature germinability

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Chinese Science Bulletin 2001年第21期46卷 1800-1804页

作者： TENG Sheng ZENG Dali QIAN Qian Kunihifo Yasufumi HUANG Danian Zhu Lihuang China National Rice Research Institute Key Lab for Rice BiologyMinistry of AgricultureHangzhou 310006China College of Life Science Zhejiang UniversityHangzhou 310029China Japan international Research Center for Agriculture Science Tsukuba 305-8686Japan Institute of genetics China Academy of SciencesBeijing 100101China

A double haploid population, derived from anther culture of F, hybrid between a typical indica and a japonica (ZYQ8/JX17), has been used to investigate the low temperature germinability (LTG) at 15℃. The low temperature germinability of two parents was significantly different. In 6-11 d, the germination percentage of ZYQ8 was higher than that of JX17. In 12-16 d, the germination percentage of JX17 was higher than that of ZYQ8. The quantitative trait loci (QTLs) of every day for low temperature germinabilityhave been mapped based on a molecular linkage map constructed from this population. In 8-11 d, qLTG-9 was identified in C397B-RZ617B on chromosome 9, the additive effect was positive, showing that, the allele from JX17 could increase low temperature germinability. In 12-16 d, qLTG-4 was mapped between RG908 and CT563 on chromosome 4, the additive effect was negative, showing that the allele from ZYQ8 could increase low temperature germinability. These two QTLs were detected at different stages,

关键词： Indica and japonica subspecies DH population low temperature germinability QTL detection dynamical analysis.

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Design of tandem genes cluster for isoflavone engineering

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Frontiers of Medicine 2009年第3期3卷 292-296页

作者： Xunli XIA Guangxiao YANG Guangyuan HE China-UK HUST-RRes genetics Engineering and Genomics Joint Laboratory Genetic Engineering International Cooperation Base of the Ministry of Science and Technology of ChinaKey Laboratory of Molecular Biophysics of Chinese Ministry of EducationCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China

In this paper,a universal effective novel method of constructing tandem genes cluster for isoﬂavone engineering was reported.A tandem genes cluster CHS-CHI-IFS(rIFS)of secondary metabolites of plant isoﬂavones was constructed by using the chalcone synthase(CHS),chalcone isomerase(CHI),and isoﬂavone synthase(IFS)(GenBank accession numbers EU526827,EU526829,EU526830)in only one recombination with the pET22b *** resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions,and the genistein-like com-ponent was detected.

关键词： isoﬂavones engineering secondary metabo-lism tandem genes cluster

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Two ABCI family transporters,OsABCI15 and OsABCI16,are involved in grain-filling in rice

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Journal of genetics and Genomics 2024年第5期51卷 492-506页

作者： Bin Ma Xiubiao Cao Xiaoyuan Li Zhong Bian Qi-Qi Zhang Zijun Fang Jiyun Liu Qun Li Qiaoquan Liu Lin Zhang Zuhua He Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop genetics and Physiology Agricultural College of Yangzhou UniversityYangzhouJiangsu 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Jiangsu Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhouJiangsu 225009China National Key Laboratory of Plant Molecular genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology&EcologyChinese Academy of SciencesShanghai 200032China Institute of Biotechnology Hangzhou Academy of Agricultural SciencesHangzhouZhejiang 310024China Joint international Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education Yangzhou UniversityYangzhouJiangsu 225009China

Seed development is critical for plant reproduction and crop yield,with panicle seed-setting rate,grain-filling,and grain weight being key seed characteristics for yield ***,few genes are known to regulate grain ***,we identify two adenosine triphosphate(ATP)-binding cassette(ABC)I-type transporter genes,OsABCI15 and OsABCI16,involved in rice *** genes are highly expressed in developing seeds,and their proteins are localized to the plasma membrane and ***,knockout of OsABCI15 and OsABCI16 results in a significant reduction in seed-setting rate,caused predominantly by the severe empty pericarp phenotype,which differs from the previously reported low seed-setting phenotype resulting from failed *** analysis indicates that OsABCI15 and OsABCI16 participate in ion homeostasis and likely export ions between filial tissues and maternal tissues during grain ***,overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants and decreases ion accumulation in brown ***,the OsABCI15/16 orthologues in maize exhibit a similar role in kernel development,as demonstrated by their disruption in transgenic ***,ourﬁndings reveal the important roles of two ABC transporters in cereal grain filling,highlighting their value in crop yield improvement.

关键词： ABC transporters Rice Grain filling Ion homeostasis Grain yield

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Generation of double-virus-resistant marker-free transgenic potato plants

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Progress in Natural Science:Materials international 2009年第5期19卷 543-548页

作者： Yunfeng Bai, Zhihua Guo, Xiaoqi Wang, Dongmei Bai, Weifeng Zhang Institute of Crop genetics, Shanxi Academy of Agricultural Sciences, Nongke Beilu No. 16, Taiyuan 030031, China [a]Institute of Crop genetics Shanxi Academy of Agricultural Sciences Nongke Beilu No. 16 Taiyuan 030031 China

Viruses are very widespread in nature, and they cause severe diseases and yield losses in potato production. The transfer of the dsRNA-producing gene could confer a high level virus resistance by specific targeting of cognate viral RNA. In this study, we constructed a marker-free expression vector of a chimeric gene derived from the coat protein sequence of Potato virus X (PVX) and the nuclear inclusion protein sequence of Potato virus Y (PVY) in the form of an intramolecular dsRNA. Then this chimeric gene was introduced into potato cv. Zihuabai, a popular variety in China, via Agrobacterium tumefaciens-mediated transformation. Marker gene-free transgenic plants resistant to both PVX and PVY were obtained and confirmed by RT-PCR and DAS-ELISA detection. Northern blot analysis showed that transgene-derived mRNA was cleaved into short interfering RNAs (siRNAs), and that the virus resistance was mediated by RNA silencing. One important aspect of the study is that the transgenic viral sequence is not translated and the actual RNA transcript is cleaved, which possibly limit the environmental risks, such as transcapsidation and recombination of the transgene with an incoming virus. In addition, the biosafety risk resulting from marker genes can be avoided because of the absence of marker genes in transgenic plants.

关键词： Solanum tuberosum Marker-free RNA silencing Double-virus resistance Transgene

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Impact of Natural Selection on Lignin and Cellulose Candidate Genes in a Natural Population of <i>Eucalyptus urophylla</i>

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Open Journal of Ecology 2014年第17期4卷 1128-1150页

作者： Létizia Camus-Kulandaivelu Bénédicte Favreau Saneyoshi Ueno Jonathan Przybyla Jean-Marc Bouvet Cirad-Bios Department AGAP Research Unit 108 “Adaptation and Breeding of Tropical and Mediterranean Plants” International Campus of Baillarguet Montpellier France Tree genetics Laboratory Department of Forest Genetics Forestry and Forest Products Research Institute Tsukuba Japan

Wood plays a major role in land ecosystems and in human activity. Better understanding the genetic basis and evolutionary implication of wood variability are thus key issues with both ecological and economical implications. The present paper addresses the question of the extending and the nature of natural selection on wood related genes in Eucalyptus urophylla, a tropical tree species with key economical importance. We conducted a genetic study on an E. urophylla population from Timor Island using a set of 17 SSR characterized on a main sample of 43 individuals and six candidate genes sequenced on a subset of 18 individuals. The candidate genes include three cellulose synthase genes (EuCesA1, EuCesA2 and EuCesA3), and three genes involved in lignin synthesis (EuCAD2, EuC4H1 and EuC4H2). Based on SSR data, the investigated population appeared to have no structure and have undergone past population expansion. Accounting for this demographic history, we were able to draw neutral expectation for polymorphism distribution on candidate genes and to determine their potential selective status. We hence identified two gene portions exhibiting unexpected polymorphism pattern, consistent with natural selection imprint.

关键词： Eucalyptus urophylla Adaptation Demography Approximate Bayesian Computation Candidate Genes

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Transcriptome-based analysis of key signaling pathways affecting the formation of primordial germ cell in chickens

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Journal of Integrative Agriculture 2024年第5期23卷 1644-1657页

作者： Ying Ding Qiong Zhi Qisheng Zuo Kai Jin Wei Han Bichun Li Key Laboratory of Animal genetics Breeding and Molecular Design of Jiangsu ProvinceCollege of Animal Science and TechnologyYangzhou UniversityYangzhou 225009China Joint international Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China Yangzhou UniversityYangzhou225009China Poultry Research Institute Chinese Academy of Agricultural Sciences/Jiangsu Institute of Poultry ScienceYangzhou 225009China

Bone morphogenetic protein 4(BMP4)can induce the formation of chicken primordial germ cells(PGCs)in vitro;however,its regulatory mechanism in poultry remains *** study aimed to use RNA-seq to analyze PGCs in chicken embryos and iPGCs induced by BMP4 in vitro,clarify the internal regulatory factors of PGCs,analyze the mechanism of the formation of PGCs,and lay a theoretical foundation for the further optimization of PGCs induction *** stem cells(ESCs),PGCs and iPGCs induced by BMP4 in vitro were *** transcriptional maps of the three cell types were studied using *** results showed 6,142 genes differentially expressed between PGCs and iPGCs,of which 2,728 were upregulated in iPGCs and 3,414 were downregulated in *** to that in ESCs,BMP4 was significantly upregulated in PGCs and *** results showed that both the TGF-βand Wnt signaling pathways were activated during the formation of PGCs in vitro and in vivo,and the activation was more significant during iPGCs induced by *** expression of Nodal,an inhibitory factor of TGF-βsignaling,was significantly decreased in PGCs and iPGCs,but was not expressed in iPGCs,which further supports our ***,the Lysosome and PI3K-AKT signaling pathways were significantly enriched in PGCs and iPGCs,***,transmission electron microscopy(TEM)results showed that the number of autolysosomes was significantly higher after the addition of BMP4,which is consistent with the KEGG ***,the number of PGCs was significantly reduced after ATG14 was interfered in vivo and in *** conclusion,this study screened out the key signaling pathways during the formation of PGCs,aiming to provide help for enriching the mechanism network regulating PGCs formation in chicken and laying a theoretical foundation for further improving the efficiency of inducing PGCs in vitro.

关键词： RNA-seq BMP4 PGCs ATG14 chicken

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