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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第12期9卷 982-989页

作者： Li-juan XIE Xing-qian YE Dong-hong LIU Yi-bin YING College of Biosystems Engineering and food Science Zhejiang University Hangzhou 310029 China

Near-infrared (NIR) spectroscopy combined with chemometrics techniques was used to classify the pure bayberry juice and the one adulterated with 10% (w/w) and 20% (w/w) water. Principal component analysis (PCA) was applied to reduce the dimensions of spectral data, give information regarding a potential capability of separation of objects, and provide principal component (PC) scores for radial basis function neural networks (RBFNN). RBFNN was used to detect bayberry juice adulterant. Multiplicative scatter correction (MSC) and standard normal variate (SNV) transformation were used to preprocess spectra. The results demonstrate that PC-RBFNN with optimum parameters can separate pure bayberry juice samples from water-adulterated bayberry at a recognition rate of 97.62%, but cannot clearly detect water levels in the adulterated bayberry juice. We conclude that NIR technology can be successfully applied to detect water-adulterated bayberry juice.

关键词： Near-infrared (NIR) spectroscopy Principal component-radial basis function neural networks (PC-RBFNN) Bayberry juice Adulteration Chemometrics technique

Antimicrobial Property of 2-Hydroxypropane-1,2,3-Tricarboxylic Acid Isolated from Citrus microcarpa Extract

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Agricultural Sciences in China 2009年第7期8卷 880-886页

作者： Seong Wei Lee Musa Najiah Department of Fishery and Aquaculture Faculty of Agrotechnology and Food Science Universiti Malaysia Terengganu

This article described antimicrobial property and structure analysis of 2-hydroxypropane-1,2,3-tricarboxylic acid isolated from the crude extract of Citrus microcarpa. Presently, there was no report on compound from C. microcarpa that possessed antimicrobial property against fish pathogenic bacteria. Therefore, in this study, the bioactive principle in C. microcarpa extract was isolated using thin layer chromatography. It＇s structure was elucidated based on nuclear magnetic resonance （NMR） spectroscopic data, such as proton NMR （1HNMR）, correlation spectroscopy, carbon 13 NMR, and heteronuclear multiple bond correlation data. This study showed that the bioactive compound isolated from C. microcarpa was 2-hydroxypropane-1,2,3-tricarboxylic acid monohydrate. The minimum inhibitory concentration （MIC） values of crude C. microcarpa extract and its bioactive component, 2-hydroxypropane-1,2,3-tricarboxylic acid as well as commercially available synthetic 2-hydroxypropane-1,2,3-tricarboxylic acid, were determined against 18 isolates of Edwardsiella tarda and 7 bacterial reference strains, namely, Escherichia coli （ATCC 25922）, Citrobacter freundii （ATCC 8090）, Aeromonas hydrophila （ATCC 49140）, Pseudomonas aeruginosa （ATCC 35032）, Streptococcus agalatiae （ATCC 13813）, E. tarda （ATCC 15947）, and Yersinia enterocolitica （ATCC 23715）, using two-fold microdilution method. The MIC values for both the natural 2-hydroxypropane-1,2,3-tricarboxylic acid and the synthetic one were ranging from 15.6 to 62.5 mg mL-1, whereas that of the crude extract was ranging from 7.8 to 31.3 mg mL-1. These findings showed that both the crude extract and its bioactive component might have potential as antimicrobial agent for aquaculture use.

关键词： antimicrobial property 2-hydroxypropane-1,2,3-tricarboxylic acid Citrus microcarpa NMR MIC

Application of NIR spectroscopy for firmness evaluation of peaches

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第7期9卷 552-557页

作者： Xia-ping FU Yi-bin YING Ying ZHOU Li-juan XIE Hui-rong XU College of Biosystems Engineering and food Science Zhejiang University Hangzhou 310029 China

The use of near infrared （NIR） spectroscopy was proved to be a useful tool for quality analysis of fruits. A bifurcated fiber type NIR spectrometer, with a detection range of 800-2500 nm by InGaAs detector, was used to evaluate the firmness of peaches. Anisotropy of NIR spectra and firmness of peaches in relation to detecting positions of different parts （including three latitudes and three longitudes） were investigated. Both spectra absorbency and firmness of peach were influenced by longitudes （i, ii, iii） and latitudes （A, B, C）. For modeling, two thirds of the samples were used as the calibration set and the remaining one third were used as the validation or prediction set. Partial least square regression （PLSR） models for different longitude and latitude spectra and for the whole fruit show that collecting several NIR spectra from different longitudes and latitudes of a fruit for NIR calibration modeling can improve the modeling performance. In addition, proper spectra pretreatments like scattering correction or derivative also can enhance the modeling performance. The best results obtained in this study were from the holistic model with multiplicative scattering correction （MSC） pretreatment, with correlation coefficient of cross-validation γcv=0.864, root mean square error of cross-validation RMSECV=6.71 N, correlation coefficient of calibration r=0.948, root mean square error of calibration RMSEC=4.21 N and root mean square error of prediction RMSEP=5.42 N. The results of this study are useful for further research and application that when applying NIR spectroscopy for objectives with anisotropic differences, spectra and quality indices are necessarily measured from several parts of each object to improve the modeling performance.

关键词： Near infrared (NIR) Anisotropy Peach Firmness Partial least square regression (PLSR)

On-site variety discrimination of tomato plant using visible-near infrared reflectance spectroscopy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 126-132页

作者： Hui-rong XU Peng YU Xia-ping FU Yi-bin YING College of Biosystems Engineering and food Science Zhejiang University Hangzhou 310029 China

The use of visible-near infrared (NIR) spectroscopy was explored as a tool to discriminate two new tomato plant varieties in China (Zheza205 and Zheza207). In this study, 82 top-canopy leaves of Zheza205 and 86 top-canopy leaves of Zheza207 were measured in visible-NIR reflectance mode. Discriminant models were developed using principal component analysis (PCA), discriminant analysis (DA), and discriminant partial least squares (DPLS) regression methods. After outliers detection, the samples were randomly split into two sets, one used as a calibration set (n=82) and the remaining samples as a validation set (n=82). When predicting the variety of the samples in validation set, the classification correctness of the DPLS model after optimizing spectral pretreatment was up to 93%. The DPLS model with raw spectra after multiplicative scatter cor- rection and Savitzky-Golay filter smoothing pretreatments had the best satisfactory calibration and prediction abilities (correlation coefficient of calibration (Rc)=0.920, root mean square errors of calibration=0.196, and root mean square errors of predic- tion=0.216). The results show that visible-NIR spectroscopy might be a suitable alternative tool to discriminate tomato plant varieties on-site.

关键词： Visible-NIR spectroscopy Tomato plant variety Discrimination Principal component analysis (PCA) Discriminant analysis (DA) Discriminant partial least squares (DPLS)

Emerging markers for antimicrobial resistance monitoring

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Chinese Chemical Letters 2023年第10期34卷 55-66页

作者： Zineng Yi Xiaona Xu Xiaohan Meng Congyu Liu Qianpeng Zhou Deyan Gong Zhengbao Zha School of food and Biological Engineering Hefei University of TechnologyHefei 230009China

The appearance and spread of antibiotic-resistant pathogens known as antimicrobial resistance(AMR)is one of the major worldwide health crises that humanity have to deal with over the next *** of the main methods for addressing AMR is the effective screening for antimicrobial insensitivity in clinical and environmental *** clinical laboratory procedures use traditional culturebased antibiotic susceptibility testing(AST)methods,which can take up to 24 h to identify which drug is suitable for the infection ***,it is vital to develop novel strategies that offer quick,simple,affordable,reliable,sensitive and accurate AMR *** for AMR markers detection could possess the essential qualities for quickly identifying resistant microorganisms and could give vital data for the selection of antibacterial drugs *** review offers a summary of the innovative application of these AMR markers detection strategies focusing on healthcare and environmental surveillance for the AMR genotypic or phenotypic assessment.

关键词： Antimicrobial resistance markers Genotypic determination Enzymes detection Antibody labeling Protein labeling

Comparative acetylome analysis reveals the potential mechanism of high fat diet function in allergic disease

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food Science and Human Wellness 2023年第3期12卷 889-894页

作者： Yanan Sun Ning Liu Huihui Wang Taiqi Qu Fazheng Ren Yixuan Li Key Laboratory of Precision Nutrition and food Quality Department of Nutrition and HealthChina Agricultural UniversityBeijing 100193China College of food Science and Engineering Gansu Agricultural UniversityLanzhou 730070China

Modern technological lifestyles promote allergic diseases,especially food *** underlying molecular mechanisms remain to be *** acetylation is one of the most important post-translational modifications,and it is involved in regulating multiple body metabolic *** study aimed to clarify the effects of a high-fat diet(HFD)on allergy risk and the underlying ***-week-old male C57 BL/6 J mice were randomly divided into two groups and fed a normal fat diet(NFD)or HFD for 24 ***,serum lipids were measured,and skeletal muscle was collected for acetylome *** with the findings in the NFD group,HFD-fed mice were obese and *** analysis also revealed 32 differentially expressed proteins between the HFD and NFD *** these,eight acetylated proteins were upregulated in the HFD *** addition,13 and 11 proteins were acetylated only in the HFD group and NFD group,*** proteins were mainly involved in regulating energy metabolism and mitochondrial *** study provides information regarding the underlying molecular mechanisms by which HFD promotes allergy.

关键词： Allergic disease High fat diet Skeletal muscle Acetylome

Antibacterial mechanism of kojic acid and tea polyphenols against Escherichia coli O157:H7 through transcriptomic analysis

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food Science and Human Wellness 2024年第2期13卷 736-747页

作者： Yilin Lin Ruifei Wang Xiaoqing Li Keren Agyekumwaa Addo Meimei Fang Yehui Zhang Yigang Yu School of food Sciences and Engineering South China University of TechnologyGuangzhou 510640China Sericulture&Agri-food Research Institute Guangdong Academy of Agricultural Sciences Key Laboratory of Functional FoodsMinistry of Agriculture and Rural AffairsGuangdong Key Laboratory of Agricultural Products ProcessingGuangzhou 510610China Research Center of food Safety and Detection College of Food Science and EngineeringSouth China University of TechnologyGuangzhou 510640China

Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in *** previous found that a combination of kojic acid and tea polyphenols exhibited better activity against *** O157:H7 than using either *** study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene *** functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against *** O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic ***,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid *** results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against *** O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.

关键词： Kojic acid Tea polyphenols Antibacterial mechanism Escherichia coli O157:H7 RNA-seq

Physical Performance of Migrant Schoolchildren with Marginal and Severe Iron Deficiency in the Suburbs of Beijing

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Biomedical and Environmental Sciences 2009年第4期22卷 333-339页

作者： JIE WANG JUN-SHENG HUO JING SUN ZHENG-XIANG NING College of Light Industry and food Sciences South China University of Technology Guangzhou 510641 Guangdong China Institute of Nutrition and food Safty Chinese Center for Disease Control and Prevention Beijing 100050 China

Objectives To investigate relationship between iron deficiency of different degrees and physical performance and habitual activity of migrant schoolchildren at the age of 11-14 years. Methods Ninety one randomly selected schoolchildren were divided into three groups according to their iron status. Iron status including hemoglobin （Hb）, serum ferritin （SF）, serum iron （SI） and sTfR was determined. Physical performance tests included maximum oxygen consumption （VO2max） and maximum work time. Energy expenditure （EE） and daily physical activity were estimated by recording 24-h heart rate （HR）. Dietary intake was assessed with frequency questionnaires, and physical activity level was estimated with frequency and physical activity questionnaires. Results Severe iron deficiency （IDA） impaired the aerobic capacity and habitual physical activity. When fat-free mass （FFM） was considered, VO2max （VO2max/FFM） was significantly lower in the iron-marginal group than in the iron-adequate groups among girls （P=0.02）, but such a deference was not found among boys （P=0.28）. Aerobic activity and EE at leisure were significantly lower in the severe iron deficient group than in the marginal iron deficient and iron adequate groups. Net HR at leisure time was correlated with lib, log SF, body weight, and FFM （P〈0.05）. Conclusion The functional effect of iron deficiency on physical performance and habitual physical activity rely on the degree of current iron deficiency. Severe iron deficiency significantly impairs both aerobic capacity and habitual physical activity. Iron-marginal deficiency impairs VO2max/FFM in girls, rather than in boys.

关键词： Iron deficiency anemia Iron deficiency Maximum oxygen consumption Heart rate Energy expenditure Physicalactivity Children of migrant workers

Modeling of moisture loss kinetics and color changes in the surface of lemon slice during the combined infrared-vacuum drying

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Information Processing in Agriculture 2018年第4期5卷 516-523页

作者： Fakhreddin Salehi Mahdi Kashaninejad Department of food Industry Machines Bu-Ali Sina UniversityHamedanIran Faculty of food Science Gorgan University of Agricultural Sciences and Natural ResourcesGorganIran

Color is one of the most important appearance attributes of food materials,since it influences consumer *** this study the effects of combined infrared-vacuum drying on the drying kinetics,moisture diffusivity,surface changes(shrinking)and color changes kinetics of lemon slices were *** the infrared lamp power and vacuum pressure influenced the drying time of lemon *** regression results showed that the quadratic model satisfactorily described the drying behavior of lemon slices with highest R value and lowest SE *** effective moisture diffusivity increased from 2.92×10^-10 and 1.58×10^-9m^2/s when the infrared lamp power was increased from 300 to *** colour parameters L^*(lightness),a^*(redness/greenness),b^*(yellowness/blueness),and DE(total colour difference)were used to estimate colour changes during drying.L^*,a^* and b^* values of dried lemon increased during *** rise in infrared power has a negative effect on the DE and with the increase of infrared radiation power it was *** kinetic models were used to fit the experimental data and the results revealed that the power model was the most suitable to describe the color change intensity(DE).

关键词： Colour parameters Image processing Infrared drying Kinetic Lemon

Strain-specific effects of Akkermansia muciniphila on the regulation of intestinal barrier

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food Science and Human Wellness 2023年第5期12卷 1526-1537页

作者： Yang Liu Qing Liu Chengcheng Zhang Jianxin Zhao Hao Zhang Wei Chen Qixiao Zhai State Key Laboratory of food Science and Technology School of Food Science and TechnologyJiangnan UniversityWuxi 214122China National Engineering Research Center for Functional food Jiangnan UniversityWuxi 214122China Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch Wuxi 214122China

Akkermansia muciniphila, one of the most promising next-generation probiotics, was reported to exhibit beneficial modulatory effects on the gut barrier. However, the strain-specific and underlying regulatory mechanisms of this species on gut barrier function were not well studied. Therefore, this study evaluated the protective effect of A. muciniphila strains on the intestinal barrier and investigated the mode of action and material basis of this modulatory effect. We first confirmed the strain-specific effects of A. muciniphila on intestinal barrier regulation and found that this phenomenon may be explained by the different abilities of strains to affect tight junction protein expression in enterocytes. Comparative genomic analysis proved that the ability of A. muciniphila to regulate the intestinal barrier was exerted in part by the functional genes(such as COG0438, COG0463, and COG2244)related to the synthesis of cellular surface proteins. The role of these surface proteins in intestinal barrier regulation was further verified by strain-comparative experiments in animal and cell models and surface protein removal trials. This study confirmed the different effects of A. muciniphila strains on gut barrier modulation and provided molecular and genetic targets for the screening of A. muciniphila strains with superior protection against gut barrier dysfunction.

关键词： Akkermansia muciniphila Intestinal barrier Strain specificity Comparative genomics Bacterial surface components

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