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Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats

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World Journal of Gastroenterology 2025年第1期31卷 93-109页

作者： Ping Zhang Ying Zhu Pu Chen Tong Zhou Zhe-Yi Han Jun Xiao Jian-Feng Ma Wen Ma Peng Zang Ying Chen Graduate School Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina Key laboratory of Aerospace Nutrition and food Engineering China Astronaut Research and Training CenterBeijing 100094China Department of Gastroenterology The Air Force Medical CenterBeijing 100142China Department of Blood Transfusion Air Force Medical CenterBeijing 100142China

BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility *** pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal ***,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal *** cells are the pacemakers of gastrointestinal *** To investigate the effects of Bifidobacterium lactis(***)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit *** The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 *** tail group was given *** BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/***&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and *** fecal flora and mucosal flora of rats in each group were detected by 16S *** Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic *** microgravity reduced the expressions of SCF and c-kit,and increased the express

关键词： Simulated microgravity Rat Intestinal flora Gastrointestinal motility Stem cell factor/c-kit signaling pathway

Olfactory receptors in neural regeneration in the central nervous system

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Neural Regeneration Research 2025年第9期20卷 2480-2494页

作者： Rafael Franco Claudia Garrigós Toni Capó Joan Serrano-Marín Rafael Rivas-Santisteban Jaume Lillo Molecular Neurobiology laboratory Department of Biochemistry and Molecular BiomedicineFacultat de BiologiaUniversitat de BarcelonaBarcelonaSpain CiberNed Network Center for Biomedical Research in Neurodegenerative Diseases Spanish National Health Institute Carlos ⅢMadridSpain School of Chemistry Universitat de BarcelonaBarcelonaSpain Department of Biochemistry and Physiology School of Pharmacy and Food SciencesUniversitat de BarcelonaBarcelonaSpain laboratory of Computational Medicine Biostatistics UnitFaculty of MedicineAutonomous University of BarcelonaCampus BellaterraBarcelonaSpain

Olfactory receptors are crucial for detecting odors and play a vital role in our sense of smell,influencing behaviors from food choices to emotional *** receptors also contribute to our perception of flavor and have potential applications in medical diagnostics and environmental *** ability of the olfactory system to regenerate its sensory neurons provides a unique model to study neural regeneration,a phenomenon largely absent in the central nervous *** gained from how olfactory neurons continuously replace themselves and reestablish functional connections can provide strategies to promote similar regenerative processes in the central nervous system,where damage often results in permanent *** the molecular and cellular mechanisms underpinning olfactory neuron regeneration could pave the way for developing therapeutic approaches to treat spinal co rd injuries and neurodegenerative diseases like Alzheimer's *** ctory receptors are found in almost any cell of eve ry orga n/tissue of the mammalian *** ectopic expression provides insights into the chemical structures that can activate olfactory *** addition to odors,olfactory receptors in ectopic expression may respond to endogenous compounds and molecules produced by mucosal colonizing *** analysis of the function of olfactory receptors in ectopic expression provides valuable information on the signaling pathway engaged upon receptor activation and the receptor's role in proliferation and cell differentiation *** review explo res the ectopic expression of olfa ctory receptors and the role they may play in neural regeneration within the central nervous system,with particular attention to compounds that can activate these receptors to initiate regenerative *** suggests that olfactory receptors could serve as potential therapeutic targets for enhancing neural repair and recovery following central nervous syste

关键词： adenosine receptors adrenergic receptors ectopic expression G proteincoupled receptors glia neurons

Tomato SlPti5 plays a regulative role in the plant immune response against Botrytis cinerea through modulation of ROS system and hormone pathways

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Journal of Integrative Agriculture 2022年第3期21卷 697-709页

作者： TANG Qiong ZHENG Xiao-dong GUO Jun YU Ting College of Biosystems Engineering and food Science National Engineering Laboratory of Intelligent Food Technology and Equipment/Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture/Zhejiang Key Laboratory for AgroFood ProcessingZhejiang UniversityHangzhou 310058P.R.China State Key laboratory of food Nutrition and Safety/Key laboratory of food Nutrition and Safety Ministry of Education/Key Laboratory of Food Quality and Health of TianjinTianjin University of Science and TechnologyTianjin 300457P.R.China

While SlPti5 has been shown to play a crucial role in the regulation of antagonistic genes in Solanum lycopersicum and Arabidopsis against pathogen infection,there have been no comprehensive studies on the effects of SlPti5 on the regulatory response mechanism of reactive oxygen species(ROS) system and hormone pathways during growth and disease resistance of tomato ***,we investigated the function of SlPti5 in the defense response of tomato against Botrytis cinerea utilizing a virus-induced gene silencing(VIGS)-based *** profile analysis showed that SlPti5 was significantly induced upon *** infection,with high expression levels in the leaves and fruit of ***-based silencing of SlPti5 inhibited early vegetative growth,increased the plant’s susceptibility to infection,promoted the development of ROS,affected the expression of genes involved in the ROS scavenging system,and attenuated the expression of genes associated with pathogenesis and the ethylene/jasmonic acid signaling *** sum,our data demonstrated that SlPti5 stimulates the immune response of tomato plant to Botrytis cinerea infection by involving the ethylene(ET)-and jasmonic acid(JA)-mediated pathways and modulating the expression of some key pathogenesis-related(PR) genes.

关键词： tomato(Solanum lycopersicum) Botrytis cinerea Sl Pti5 immune response ET-and JA-mediated signaling pathways pathogenesis-related proteins

A novel mitigator of enzymatic browning--hawthorn leaf extract and its application in the preservation of fresh-cut potatoes

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food Quality and Safety 2021年第3期5卷 247-255页

作者： Liping Qiao Hailin Wang Jinsheng Shao Laifeng Lu Jinhu Tian Xia Liu State Key laboratory of food Nutrition and Safety Ministry of EducationTianjin Key Laboratory of Food Nutrition and SafetyCollege of Food Science and EngineeringTianjin University of Science&TechnologyTianjinChina College of Biosystems Engineering and food Science National-Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agro-Food ProcessingZhejiang Engineering Laboratory of Food Tech no logy and EquipmentFuli Institute of Food ScienceZhejiang Un iversityHangzhouChina Tianjin Gasin-DH Preservation Technology Co.Ltd. TianjinChina

Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible *** and Methods:Fresh-cut potatoes were treated with different concentrations(0.01%,0.05%,and 0.1%)of hawthorn leaf extract and preserved at 4 C for 8 *** appears nee and colour of potato slices were evaluated,along with the content of the phenol,malondialdehyde(MDA),and hydrogen peroxide(H2O2)during cold ***,the activities of polyphenol oxidase(PPO),peroxidase(POD),phenylalanine ammonia-lyase(PAL),lipoxygenase(LOX),catalase(CAT),superoxide dismutase(SOD),and the antioxidant capacity were determ in ***,the composition of hawthorn leaf extract was analyzed by high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Results:The addition of hawthorn leaf extract effectively delayed the browning *** not only enhanced the CAT activity and antioxidant capacity but also reduced the LOX activity and accumulation of MDA and H_(2)O_(2).Meanwhile,the activities of PPO,POD,and PAL as well as the content of phe nol were con ***,25 phe nols,34 flavonoids,and 5 proa nthocya nidins were identified through high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),ideluding caffeic acid,quercetin and ***:Hawthorn leaf extract significantly alleviated the browning of fresh-cut *** could serve as a n atural an tibrowning alter native by stabilizing the membra ne and modulating reactive oxygen species and redox reactions.

关键词： Hawthorn leaf extract enzymatic browning fresh-cut potatoes.

Nutritional and microbiological evaluations of chocolate-coated Chinese chestnut (Castanea mollissima) fruit for commercial use

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 675-683页

作者： Mahamadou E. GOUNGA State Key laboratory of food Science & Technology School of Food Science & Technology Jiangnan University

In recent years, China has become an increasingly important and the largest chestnut producer in the world. This study aimed to evaluate the nutritional value and microbiological quality of the roasted freeze-dried Chinese chestnut (Castanea mollissima) (RFDC) coated with dark chocolate (DCC) and milk chocolate (MCC) for industrial use and commercial consumption. Chocolate coating significantly improved the nutritional value of chestnut. RFDC had high levels of starch (66.23%) and fibers (3.85%) while DCC and MCC contained significantly high amounts of sucrose, protein, fat and minerals. Furthermore, the protein content doubled in MCC rather than in DCC. This could be attributed to the different formulations in the two products. Milk powder and whey protein constituted the source of protein in MCC while cocoa powder added to MCC formulation constituted an additional source of minerals. The amino acid profile showed differences in amino acid composition related to the sample’s protein content, indicating their good nutritional quality. The moisture contents in all RFDC, DCC and MCC were suitable for industrial processing. These results provide information about the additional nutrients of chocolate-coated chestnut and confirm that the product is an interesting nutritional food. The combination of freeze-drying and chocolate-coating generally results in greater reductions on microbiological loads, extending shelf life of harvested chestnut for commercial application. This is an alternative strategy to add value to chestnut, minimizing the significant losses in harvested fruits and providing a wider range of choices of new products to the consumer disposal.

关键词： Chestnut Coating Dark chocolate Milk chocolate Nutritional qualities Microbiological analysis

The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

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Chinese Journal of Oceanology and Limnology 2009年第4期27卷 703-707页

作者：郭永超李振兴林洪 food Safety laboratory Ocean University of China

Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp （Penaeus vannamei） was subjected to digestion in SGF （simulated gastric fluid） and SIF （simulated intestinal fluid） for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen a 1 exhibited strong resistance to digestive fluids, and all yielded fragments （33 kD, 23 kD, and 14 kD） showed allergic activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy.

关键词： Pen a 1 allergenicity SGF SIF digestion

Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B_(6) in energy drinks and dietary supplements

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Nano Research 2022年第3期15卷 2479-2488页

作者： Lu Zeng Xinxin Xu Shanshan Song Liguang Xu Liqiang Liu Jing Xiao Chuanlai Xu Hua Kuang State Key laboratory of food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China NHC Key laboratory of food Safety Risk Assessment China National Center for Food Safety Risk AssessmentBeijing 100022China

We designed and synthesized novel haptens to produce monoclonal antibodies(mAb)against vitamin B_(6)(VB_(6)).A group-specific mAb(2F9)that recognized pyridoxine(PN),pyridoxamine(PM),and pyridoxal(PL)was prepared using a homologous strategy with 50%maximal inhibitory concentration(IC_(50))values of 106.60,250.57,and 400.11 ng/mL,*** on this,a gold nanoparticles(AuNPs)-based immunochromatographic strip(ICS)test was established for the detection of VB_(6) in energy drinks and B-vitamin complex *** developed ICS test results could be semi-quantitatively evaluated by the naked eye within 10 min,and displayed the visual limit of detection(vLOD)values of 250,500,and 1,000 ng/mL for PN,PM,and PL,*** quantitative analysis,the results obtained by strip reader,with calculated LOD values for PN,PM,and PL were 14.10,55.58,and 56.25 ng/mL,*** energy drinks and B-vitamin complex tablet samples were detected by the strips and the results were confirmed with high-performance liquid ***,the developed AuNPs-based immunochromatographic sensor was suitable and promising for the group-specific recognition and rapid detection of VB6 in fortified foods and dietary supplements.

关键词： vitamin B_(6) monoclonal antibody immunochromatographic strip test

Inhibitory effect of reuterin-producing Limosilactobacillus reuteri and edible alginate-konjac gum film against foodborne pathogens and spoilage microorganisms

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food Bioscience 2023年第2期52卷 912-920页

作者： F.J.Rodrigues M.F.Cedran J.L.Bicas H.H.Sato food Biochemistry laboratory Department of Food Science and NutritionSchool of Food EngineeringUniversity of CampinasCampinasSPBrazil food Biotechnology laboratory Department of Food Science and NutritionSchool of Food EngineeringUniversity of CampinasCampinasSPBrazil

The possible inhibitory effects against foodborne pathogens and spoilage microorganisms of two strains(DSM 20016 and DSM 17938)of reuterin-producing Limosilactobacillus reuteri,and an edible alginate-konjac gum film containing *** cells and in situ produced reuterin was *** in vitro antimicrobial screening study indicated that both *** strains and the edible film showed inhibitory effects against ***,*** and ***,and towards the fungi ***,***,***,and *** addition,*** DSM 20016 was able to produce reuterin in situ(46.67 mmol/L)in alginate-konjac gum film-forming solution through anaerobic glycerol ***,after the edible film production by extrusion technique,both *** and reuterin were successfully trapped(98 and 67.6%,respectively).Further studies must be conducted to detect the exact metabolites related to the inhibition effects against foodborne pathogens and spoilage microorganisms.

关键词： 3-Hydroxypropionaldehyde Lactobacillus reuteri Antimicrobial activity Edible film Konjac gum

A heteropolysaccharide from Rhodiola rosea L.:preparation,purification and anti-tumor activities in H22-bearing mice

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food Science and Human Wellness 2023年第2期12卷 536-545页

作者： Yaru Wu Qing Wang Huiping Liu Lulu Niu Mengyu Li Qi Jia State Key laboratory of food Nutrition and Safety Key Laboratory of Food Nutrition and SafetyMinistry of Education of ChinaCollege of Food Science and EngineeringTianjin University of Science and TechnologyTianjin 300457China

Numerous polysaccharides isolated from plants have been used to augment traditional drugs in the treatment of *** order to explore the influence to hepatocellular carcinoma,a novel cold water-soluble polysaccharide was separated from Rhodiola rosea ***(RLP)and then its structure and anti-cancer activities were *** chemical compositions and high performance gel permeation chromatography(HPGPC)results indicated that RLP was an acid heteropolysaccharide with the molecular weight of about1.15×10~6 ***,ion chromatography(IC),Fourier transform infrared(FT-IR)and nuclear magnetic resoance(NMR)further indicated that RLP was main composed of→2,4)-α-Rha(1→,→5)-α-L-Araf-(1→,α-D-Glu,→6)-β-D-Galp-(1→,β-D-Man and→4)-α-GalpA-(1→.In vivo antitumor activities of RLP were carried out by using H22 tumor-bearing mice *** results shown that RLP(100 and 300 mg/kg)could inhibit tumor growth of H22 cells from 23.59%to 45.52%and protect thymuses and spleen without *** addition,according to cell cycle,AV-FITC/PI and JC-1,RLP could induce dose-dependent apopto sis of H22 cells via S phase arrested which was through a mitochondrial related *** data indicated that RLP has a broader application prospect in anti-tumor preparations.

关键词： Polysaccharide Antitumor activity Rhodiola rosea L. Structure analysis