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Effects of different doses of glucose and fructose on central metabolic pathways and intercellular wireless communication networks in humans

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food Science and Human Wellness 2024年第4期13卷 1906-1916页

作者： Dingqiang Lu Yujiao Liu Miao Zhao Shuai Yuan Danyang Liu Xinqian Wang Yixuan Liu Yifei Zhang Ming Li Yufeng Lü Guangchang Pang Ruijuan Ren College of biotechnology&food Science Tianjin University of CommerceTianjin Key Laboratory of Food BiotechnologyTianjin 300314China Tianjin Institute for food Safety Inspection Technology Tianjin 300134China

Fructose and glucose are often widely used in food processing and may contribute to many metabolic *** observe the effects of different doses of glucose and fructose on human metabolism and cellular communication,volunteers were given low,medium,and high doses of glucose and *** cytokines,glucose,lactate,nicotinamide adenine dinucleotide(NADH)and metabolic enzymes were assayed,and central carbon metabolic pathway networks and cytokine communication networks were *** results showed that the glucose and fructose groups basically maintained the trend of decreasing catabolism and increasing anabolism with increasing *** with glucose,low-dose fructose decreased catabolism and increased anabolism,significantly enhanced the expression of the inflammatory cytokine interferon-γ(IFN-γ),macrophage-derived chemokine(MDC),induced protein-10(IP-10),and eotaxin,and significantly reduced the activity of isocitrate dehydrogenase(ICDH)and pyruvate dehydrogenase complexes(PDHC).Both medium and high doses of fructose increase catabolism and anabolism,and there are more cytokines and enzymes with significant ***,multiple cytokines and enzymes show strong relevance to metabolic regulation by altering the transcription and expression of enzymes in central carbon metabolic ***,excessive intake of fructose should be reduced to avoid excessive inflammatory responses,allergic reactions and autoimmune diseases.

关键词： Fructose Glucose Central carbon metabolic pathway Metabolic enzymes Cytokine network

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The interaction mechanism between zein and folic acid in alkaline aqueous solutions:an experimental and molecular simulation study

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food Quality and Safety 2024年第1期8卷 166-178页

作者： Jun Zhu Yiling Yao Yuying Liao Lala Cai Wenqi Zhang Nuo Hong Wenlin Sun Chunhua Xiong Lanying Yao School of food Science and biotechnology Zhejiang Gongshang UniversityHangzhouChina

Objectives:Folic acid is a vitamin that is not highly soluble in water and is sensitive to the ***,it is important to find suitable *** study aimed to exemplify the interaction of folic acid with zein in alkaline aqueous solutions and shed light on how zein can be used as a carrier for folic *** and Methods:Zein and folic acid were separately dissolved in NaOH solutions with a PH of *** solution and folic acid solution were combined in specific *** methods including multi-spectroscopy,dynamic light scattering,and electron microscopy combining with molecular dynamics simulations were used to study the interaction mechanism between zein and folic acid in alkaline aqueous ***:Fluorescence spectroscopy showed that the quenching of zein by folic acid was mainly static,and the main driving force behind this interaction was van der Waals forces and hydrogen *** formation of zein–folic acid complexes was confirmed by ultraviolet–visible spectroscopy,Fourier transform infrared spectroscopy,and circular dichroism *** results also showed that the structure of zein changed when it interacted with folic *** light scattering analysis revealed that the addition of folic acid caused proteins to *** aggregates of the complexes had an irregular shape and were large,as observed by scanning electron microscopy and transmission electron *** simulation was used to further investigate the interaction *** to these findings,the folic acid molecule interacted with zein in a shallow recess near the protein *** dominant forces at play in the zein–folic acid interaction were van der Waals forces and electrostatic forces,including hydrogen ***:The zein alkali-soluble system is very suitable for folic acid delivery.

关键词： Zein folic acid alkaline aqueous solutions molecular simulation interaction mechanism.

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Current status and challenges for cell-cultured milk technology: a systematic review

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Journal of Animal Science and biotechnology 2024年第5期15卷 1778-1792页

作者： Hyuk Cheol Kwon Hyun Su Jung Vahinika Kothuri Sung Gu Han Department of food Science and biotechnology of Animal Resources Konkuk UniversitySeoulRepublic of Korea

Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional *** most cellular agriculture is predominantly centered on the production of cultured meat,there is a growing demand for an understanding of the production techniques involved in dairy products within cellular *** review focuses on the current status of cellular agriculture in the dairy sector and technical challenges for cell-cultured milk *** agriculture technology in the dairy sector has been classified into fermentation-based and animal cell culture-based cellular ***,various companies synthesize milk components through precision fermentation ***,several startup companies are pursuing animal cell-based technology,driven by public concerns regarding genetically modified organisms in precision fermentation ***,this review offers an up-to-date exploration of animal cell-based cellular agriculture to produce milk components,specifically emphasizing the structural,functional,and productive aspects of mammary epithelial cells,providing new information for industry and academia.

关键词： Cell culture system Cell-cultured milk Mammary epithelial cells Precision fermentation

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Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses

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Synthetic and Systems biotechnology 2024年第3期9卷 470-480页

作者： Kexin Ren Qiang Wang Jianghua Chen Hengwei Zhang Zhoule Guo Meijuan Xu Zhiming Rao Xian Zhang Key Laboratory of Industrial biotechnology of the Ministry of Education School of BiotechnologyJiangnan UniversityWuxiJiangsu214122China Yixing Institute of food and biotechnology Co. LtdYixing214200China

Microbial cell factories utilize renewable raw materials for industrial chemical production,providing a promising path for sustainable *** subtilis is widely used in industry for its food safety properties,but challenges remain in the limitations of microbial *** study proposes a novel strategy based on lifespan engineering to design robust *** chassis cells to supplement traditional metabolic modification strategies that can alleviate cell autolysis,tolerate toxic substrates,and get a higher mass transfer *** modified chassis cells could produce high levels of L-glutaminase,and tolerate hydroquinone to produceα-arbutin *** a 5 L bioreactor,the L-glutaminase enzyme activity of the final strain CRE15TG was increased to 2817.4±21.7 U m L^(-1),about 1.98-fold compared with that of the wild ***α-arbutin yield of strain CRE15A was increased to 134.7 g L^(-1),about 1.34-fold compared with that of the *** our knowledge,both of the products in this study performed the highest yields reported so *** chassis modification strategy described in this study can Improve the utilization efficiency of chassis cells,mitigate the possible adverse effects caused by excessive metabolic modification of engineered strains,and provide a new idea for the future design of microbial cell factories.

关键词： Bacillus subtilis Chassis cell Lifespan engineering Robustness Industrial production

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Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch

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Synthetic and Systems biotechnology 2024年第3期9卷 577-585页

作者： Haoyu Guo Rongzhen Tian Yaokang Wu Xueqin Lv Jianghua Li Long Liu a b Guocheng Du Jian Chen Yanfeng Liu Key Laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China Science Center for Future foods Jiangnan UniversityWuxi214122China Engineering Research Center of Ministry of Education on food Synthetic biotechnology Jiangnan UniversityWuxi214122China Jiangsu Province Engineering Research Center of food Synthetic biotechnology Jiangnan UniversityWuxi214122China

Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microbial cell factories for efficient bioproduction in synthetic *** comparison to plasmid-based multi-copy expression,the utilization of chromosomal multi-copy genes offers increased stability of expression level,diminishes the metabolic burden on host cells,and enhances overall genetic *** this study,we developed the“BacAmp”,a stabilized gene integration expression and copy number amplification system for high-level expression in Bacillus subtilis,which was achieved by employing a combination of repressor and non-natural amino acids(ncAA)-dependent expression system to create a reversible switch to control the key gene recA for homologous *** the reversible switch is turned on,genome editing and gene amplification can be ***,the reversible switch was turned off therefore stabilizing the gene copy *** stabilized gene amplification system marked by green fluorescent protein,achieved a 3-fold increase in gene expression by gene amplification and maintained the average gene copy number at 10 after 110 *** we implemented the gene amplification system for the regulation of N-acetylneuraminic acid(NeuAc)synthesis,the copy number of the critical gene increased to an average of 7.7,which yielded a 1.3-fold NeuAc *** research provides a new avenue for gene expression in synthetic biology and can be applied in metabolic engineering in ***.

关键词： Bacillus subtilis Stabilized gene amplification system Homologous recombination recA

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Enhanced extracellular production of alpha-lactalbumin from Bacillus subtilis through signal peptide and promoter screening

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food Science and Human Wellness 2024年第4期13卷 2310-2316页

作者： Yuqi Zhu Pengdong Sun Chunjian Li Yu Zhang Yu Wang Jingyuan Li Yanfeng Liu Jian Chen Yang Deng College of food Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Special food Processing(Co-construction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Qingdao Special food Research Institute Qingdao 266109China Science Center for Future foods Engineering Research Center of Ministry of Education on Food Synthetic BiotechnologyJiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Alpha-lactalbumin(α-LA)is a major whey protein found in breast milk and plays a crucial role in the growth and development of *** this study,Bacillus subtilis RIK1285 harboring AprE signal peptide(SP)was selected as the original strain for the production ofα-*** was found thatα-LA was identified in the pellet after ultrasonic disruption and centrifugation instead of in the fermentation *** original strain most likely only producedα-LA intracellular,but not *** improve the expression and secretion ofα-LA in RIK1285,a library of 173 homologous SPs from the *** 168 genome was fused with target LALBA gene in the pBE-S vector and expressed extracellularly in *** YjcN was determined to be the best signal *** in supernatant were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and purified by nickel column to calculate the highest yield signal *** addition,different promoters(P_(aprE),P_(43),and P_(glv))were compared and *** results indicated that the strain RIK1285-pBE-P_(glv)-YjcN-LALBA had the highestα-LA yield,reaching 122.04μg/*** study demonstrates successful expression and secretion of humanα-LA in *** and establishes a foundation for simulating breast milk for infant formulas and developing bioengineered milk.

关键词： Bacillus subtilis Alpha-lactalbumin Bioengineering milk Signal peptide Promoter screening

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Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum

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Synthetic and Systems biotechnology 2024年第1期9卷 99-107页

作者： Chunjian Li Pengdong Sun Guoqing Wei Yuqi Zhu Jingyuan Li Yanfeng Liu Jian Chen Yang Deng College of food Science and Engineering Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Special food Processing(Co-construction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Shandong Technology Innovation Center of Special food Qingdao 266109China Qingdao Special food Research Institute Qingdao 266109China Qingdao Nuoan Baite biotechnology Co. Ltd.Qingdao 266109China Science Center for Future foods Engineering Research Center of Ministry of Education on Food Synthetic Biotechnologyand Jiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Creatine is a naturally occurring derivative of an amino acid commonly utilized in functional foods and ***,the current industrial synthesis of creatine relies on chemical processes,which may hinder its utilization in certain ***,a biological approach was devised that employs whole-cell biocatalysis in the bacterium Corynebacterium glutamicum,which is considered safe for use in food production,to produce safe-for-consumption *** objective of this study was to identify a guanidinoacetate N-methyltransferase(GAMT)with superior catalytic activity for creatine *** employing whole-cell biocatalysis,a gamt gene from Mus caroli(Mcgamt)was cloned and expressed in *** ATCC 13032,resulting in a creatine titer of 3.37 g/***,the study employed a promoter screening strategy that utilized nine native strong promoters in *** to enhance the expression level of *** highest titer was achieved using the P1676 promoter,reaching 4.14 g/*** conditions of whole-cell biocatalysis were further optimized,resulting in a creatine titer of 5.42 g/*** is the first report of successful secretory creatine expression in ***,which provides a safer and eco-friendly approach for the industrial production of creatine.

关键词： Creatine Corynebacterium glutamicum Whole-cell biocatalysis Guanidinoacetate N-Methyltransferase food additive

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Fatty acids derived from oviposition systems guide female black soldier flies(Hermetia illucens)toward egg deposition sites

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Insect Science 2024年第4期31卷 1231-1248页

作者： Patrick Klüber Emna Arous Jeanny Jerschow Marco Fraatz Daniel Bakonyi Martin Rühl Holger Zorn Department Bioresources Fraunhofer Institute for Molecular Biology and Applied EcologyGiessenGermany Institute of food Chemistry and food biotechnology Justus Liebig University GiessenGiessenGermany

The black soldier fly,Hermetia illucens,comes with big promises for industrial purposes since its larvae feed polyphagously on a broad spectrum of organic ***,research focusing on adult flies is scarce,which is inconsistent with their reproductive relevance within the rearing *** particular,directed oviposition is a challenge in artificial ***,decomposing organic matter is commonly used as oviposition substrate,which has extensive potential for improvement in view of the lack of standardization and the risk of microbial ***,we identified three fatty acids and one fatty acid methyl ester derived from the surface of old oviposition sites and targeted to elucidate their effect on preference behavior and oviposition site selection using Y-olfactometry and prepared oviposition sites,*** to tetradecanoic acid attracted gravid females and stimulated oviposition most strongly,while decanoic acid demonstrated a repulsive *** kept in mixed-sex populations were attracted by tetradecanoic acid,resulting in a higher egg mass found in the compound box(3.0–11.4 fold),a≥2.3 fold reduction of nonspecifically deposited eggs,and the highest total egg ***,decanoic and dodecanoic acid caused females to lay a greater proportion of eggs nonspecifically outside both *** data suggest that fatty acids,especially tetradecanoic acid,are important cues for oviposition site selection in black soldier *** order to achieve a directed oviposition behavior,the role of further short-and long-chain fatty acids as attractants should be examined.

关键词： black soldier fly Hermetia illucens fatty acids insects as food and feed oviposition oviposition site selection tetradecanoic acid

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Lactobacillus plantarum CCFM1180 attenuates obesity induced by estrogen deficiency by activating estrogen receptor alpha in abdominal adipose tissue and regulating gut microbiota-derived metabolites

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food Science and Human Wellness 2024年第2期13卷 765-777页

作者： Qian Chen Chunxia Mei Min Guo Botao Wang Haiqin Chen Jianxin Zhao Gang Wang Wei Chen State Key Laboratory of food Science and Resources Jiangnan UniversityWuxi 214122China School of food Science and Technology Jiangnan UniversityWuxi 214122China National Engineering Research Center for Functional food Jiangnan UniversityWuxi 214122China (Yangzhou)Institute of food biotechnology Jiangnan UniversityYangzhou 225004China Bloomage biotechnology Co. Ltd.Jinan 250000China

Lipid metabolism disorders commonly occur during *** deficiency has been shown to lead to excessive energy intake and abnormal lipid metabolism in ovariectomized rats,resulting in *** exhibit anti-obesity properties,and their underlying mechanism has been widely *** this study,we demonstrated the metabolic benefits of Lactobacillus plantarum CCFM1180 in suppressing appetite,controlling body weight,correcting obesity-induced abnormalities,enhancing liver lipid metabolism,and protecting liver function in estrogen-deficient *** mechanisms associated with the anti-obesity and anti-dyslipidemia effects of CCFM1180 on estrogen-deficient rats were *** results showed that CCFM1180 dramatically reduced food intake by activating the expression of estrogen receptor alpha(ERα)and increasing the level of leptin in abdominal adipose *** changes,combined with the increased butyrate concentration and recovered bile acid structure,helped enhance lipid ***,CCFM1180 treatment was found to be safer than exogenous estrogen ***,*** CCFM1180 could be considered a new therapeutic strategy for preventing and alleviating menopausal lipid abnormalities.

关键词： Bile acid Gut microbiota Menopause Ovariectomy Short chain fatty acid

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Combined Insights from Leachate Structure and Microstructure Characteristics for Eating Quality of Convenience Rice Processed by Super-Heated and Pressurized Steam Technologies

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Rice science 2024年第4期31卷 475-488,I0035,I0036页

作者： Mingyo HA Hyo-Young JEONG Ju Hun LEE Hyun-Jung CHUNG Division of food and Nutrition Chonnam National UniversityGwangju 61186South Korea Department of biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoul 02841South Korea Division of food Science&Culinary Arts Shinhan UniversityGyeonggi 11644South Korea

Convenience rice has become widely popular due to its easy availability for cooking. This study investigated the starch structure and composition of leachate and the microstructure of reheated convenience rice using novel processing technologies: super-heated steaming(SHS), auto-electric cooking(AEC), and pressurized-steam cooking(PSC). Additionally, the effect of two different target water contents(58% and 63%) was also evaluated. The PSC_63% sample had the highest total solids and amylopectin amount in the leachate. The amylopectin amount in the leachate differed significantly based on the targeted water content. Morphological characterization revealed that the swelling of starch and the coated layer on the surface of rice grains were most pronounced in the PSC_63% sample due to the pressure processing. The textural hardness of the AEC_58% sample was much higher than that of the other samples. The PSC_63% sample had the highest textural adhesiveness value, which can be attributed to the highest amylopectin amount in the leachate. Sensory characterization showed that the PSC_63% sample had the highest glossiness, whiteness, moistness, and overall acceptability. The principal component analysis score plots presented substantial differences in the leachate and textural and sensory characteristics of reheated convenience rice among the different processing technologies.

关键词： reheated convenience rice starch structure leachate multi-scale structure eating quality processing technology

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