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Purification,chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第6期11卷 437-442页

作者： Xiu-ju DU Jing-song ZHANG Yan YANG Qing-jiu TANG Wei JIA Ying-jie PAN Institute of Edible Fungi Shanghai Academy of Agricultural Sciences Shanghai 201106 China College of Life Science Liaocheng University Liaocheng 252059 China College of food Science Shanghai Ocean University Shanghai 200090 China

Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from Tremella aurantialba fruit *** crude fractions(TAP50w,TAP10-50w,and TAP1-10w),five semi-purified fractions(TAPA-TAPE),and one purified fraction(TAPA1) were obtained.A sulfated derivative of TAPA1(TAPA1-s) was prepared by chemical *** immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation *** the three crude fractions tested,cell prolifera-tion rates were increased most by ***,TAPA1-s was markedly more stimulatory than TAPA1,indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.

关键词： Tremella aurantialba Purification Polysaccharide Sulfation Immunostimulating activity

Competitive effect, but not competitive response, varies along a climatic gradient depending on tree species identity

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Forest Ecosystems 2024年第2期11卷 142-151页

作者： Teresa Valor Lluís Coll David I.Forrester Hans Pretzsch Miren del Río Kamil Bielak Bogdan Brzeziecki Franz Binder Torben Hilmers Zuzana Sitková Roberto Tognetti Aitor Ameztegui Department of Agricultural and Forest Sciences and Engineering(DCEFA) University of LleidaAv.Alcalde Rovira Roure 191E25198 LleidaSpain Joint Research Unit CTFC-AGROTECNIO-CERCA Ctra.Sant LlorençKm.225280SolsonaSpain Swiss Federal Institute of Forest Snow and Landscape Research WSLZürcherstrasse 1118903BirmensdorfSwitzerland CSIRO Land and Water GPO Box 1700CanberraACT2601Australia Chair of Forest Growth and Yield Science School of Life Sciences WeihenstephanTechnical University of MunichHans-Carl-Von-Carlowitz-Platz 285354FreisingGermany Forest Research Center INIA-CSICCtra.A Coruña Km 7.528040MadridSpain IuFOR Sustainable Forest Management Research InstituteUniversity of Valladolid&INIASpain Department of Silviculture Institute of Forest SciencesWarsaw University of Life SciencesNowoursynowska 159/3402776WarsawPoland Bavarian State Institute of Forestry(LWF) Hans-Carl-von-Carlowitz-Platz 1D-85354FreisingGermany National Forest Centre Forest Research InstituteT.G.Masaryka 2296001ZvolenSlovakia Faculty of Agricultural Environmental and Food SciencesFree University of Bozen-Bolzano Piazza Università1 I-39100Bolzano

Background: Understanding the role of species identity in interactions among individuals is crucial for assessing the productivity and stability of mixed forests over time. However, there is limited knowledge concerning the variation in competitive effect and response of different species along climatic gradients. In this study, we investigated the importance of climate, tree size, and competition on the growth of three tree species: spruce(Picea abies), fir(Abies alba), and beech(Fagus sylvatica), and examined their competitive response and effect along a climatic ***: We selected 39 plots distributed across the European mountains with records of the position and growth of 5,759 individuals. For each target species, models relating tree growth to tree size, climate and competition were proposed. Competition was modelled using a neighbourhood competition index that considered the effects of inter-and intraspecific competition on target trees. Competitive responses and effects were related to *** methods and information theory were used to select the best ***: Our findings revealed that competition had a greater impact on target species growth than tree size or climate. Climate did influence the competitive effects of neighbouring species, but it did not affect the target species? response to competition. The strength of competitive effects varied along the gradient, contingent on the identity of the interacting species. When the target species exhibited an intermediate competitive effect relative to neighbouring species, both higher inter-than intraspecific competitive effects and competition reduction occurred along the gradient. Notably, species competitive effects were most pronounced when the target species' growth was at its peak and weakest when growing conditions were far from their ***: Climate modulates the effects of competition from neighbouring trees on the target tree and not the susceptibility of th

关键词： Competition coefficient Competition reduction Interspecific competition Intraspecific competition Mixing effects Mixed species forest Neighbourhood models Plant-plant interactions

Evaluation of Some Secondary Metabolites and Determination of the Antioxidant Potential of Different Extracts from the Plant of <i>Pteridium aquilinum</i>

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American Journal of Analytical Chemistry 2021年第12期12卷 506-519页

作者： Arnaud W. G. Tamba Sompila Aimé B. Madiélé Mabika Nadia P. G. Pambou-Tobi Tsiba Gouollaly Jacques E. Moussounga Gloria L. L. Bissombolo N’simba Roniche Nguie Louis Matos National Institute for Research in Engineering Sciences Innovation and Technology Scientific City Brazzaville Congo Laboratory of food and Medical Bioprocesses National Polytechnic School Marien Ngouabi University Brazzaville Congo National Polytechnic School Marien Ngouabi University Brazzaville Congo Plant and Life Chemistry Unit Faculty of Science and Technology Marien Ngouabi University Brazzaville Congo National Institute for Research in Health Sciences Scientific City Brazzaville Congo Pole of Excellence in food and Nutrition Faculty of Science and Technology Marien Ngouabi University Brazzaville Congo

The present study aims to make an evaluation of some secondary metabolites and determination of the antioxidant potential of P. aquilinum plant extracts obtained by means of a simple and rapid TLC method. The latter revealed the presence of terpenes, sterols, steroids, flavonoids, polyphenols, saponins, sugars and amino acids. The evaluation of the potential antioxidant was assessed on phenolic and flavonoid compounds. These compounds’ dosages revealed different levels, but the highest antioxidant activity was found in the hydro-ethanol extract followed by the aqueous extract. Among the two families of evaluated antioxidants, phenolic compounds were found to be higher in the hydro-ethanolic extract (75.18 mgEAG/gMS), followed by the aqueous extract (66.78 mgEAG/gMS) and lower in the ethanolic extract (12.39 mgEAG/gMS). Whereas flavonoids, less significantly elevated, showed values of 2.58 mgECa/gMS for the hydro-ethanolic extract, 2.24 mgECa/gMS for the aqueous extract and 1.58 mgECa/gMS for the ethanolic extract. However, the antiradical activity was also evaluated. Contrary to the antioxidant activity, the most important antiradical activity was observed on the hydroethanolic extract with a rate of 3.61 mg/mL, then a weak activity on the aqueous and ethanolic extracts respectively 6.18 mg/mL and 15.81 mg/mL, then less important on the aqueous and hydro-ethanolic extracts respectively at levels of 6.18 mg/mL and 3.61 mg/mL.

关键词： Evaluation Extracts Secondary Metabolites Potential Antioxidant Pteridium aquilinum

Effects of Edible Coatings on Quality and Antioxidant Activity of Zizyphus Jujuba Miller cv. Dongzao During Storage

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Transactions of Tianjin University 2017年第1期23卷 51-61页

作者： Xiaohong Kou Yunfei Li Jiyun Wu Qiong Chen Zhaohui Xue School of Chemical Engineering and technology Tianjin University Tianjin 300354 China

The development of edible coatings has been lauded with respect to their safety and effectiveness. In this study, we researched the effects of edible coatings (2% CaCl2, 1% chitosan and 1% pullulan) on the nutrient content and antioxidant abilities of jujube (Zizyphus jujuba Miller cv. Dongzao). Using the new analysis technique for order of preference by similarity to ideal solution (TOPSIS), we evaluated the effects of these coatings. Compared with the control fruit group, test results showed that coating treatment significantly delayed fruit senescence. Specifically, CaCl2treatment not only maintained fruit storage quality and antioxidant activity but also restrained the production and accumulation of malondialdehyde in jujube. Chitosan treatment delayed decreases in secondary metabolites and superoxide dismutase and catalase activity. Pullulan coatings performed better in terms of proanthocyanidin and cyclic adenosine monophosphate (cAMP). We also used TOPSIS to evaluate the preservation effect of different film coatings and found 2% CaCl2to be the best treatment for jujube, followed by 1% chitosan and 1% pullulan. Based on the appropriate materials and concentration of the film coatings, edible coatings have the potential to retain the quality and antioxidant capacity of the Chinese jujube cv. Dongzao. © 2016, Tianjin University and Springer-Verlag Berlin Heidelberg.

关键词： Agents Antioxidants Chitin Chitosan Enzymes Flavonoids food additives Fruits Metabolites Oxygen Plants (botany) Storage (materials)

Seed caching and cache pilferage by three rodent species in a temperate forest in the Xiaoxinganling Mountains

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Zoological Research 2013年第1期34卷 13-18页

作者： Ming-Ming ZHANG Zhen SHEN Guo-Qiang LIU Xian-Feng YI College of Agriculture Henan University of Science and TechnologyLuoyang 471003China

Although differences in food-hoarding tactics both reflect a behavioral response to cache pilferage among rodent species and may help explain their coexistence,differentiation in cache pilfering abilities among sympatric rodents with different hoarding strategies is seldom *** carried out semi-natural enclosure experiments to investigate seed hoarding tactics among three sympatric rodent species(Tamias sibiricus,Apodemus peninsulae and Clethrionomys rufocanus)and the relationship of their pilfering abilities at the inter-and intraspecific *** results showed that *** exhibited a relatively stronger pilfering ability than *** and ***,as indicated by its higher recovery rate of artificial *** *** showed a medium pilfering ability and *** displayed the lowest *** also noted that both cache size and cache depth significantly affected cache recovery in all three *** scatter-hoarded more seeds than it larder-hoarded,*** larder-hoarded more than scatter-hoarded,and *** acted as a pure *** ***,individuals with lower pilfering abilities tended to scatter hoard seeds,indicating an intraspecific variation in hoarding ***,these results indicated that sympatric rodent species seem to deploy different food hoarding tactics that allow their coexistence in the temperate forests,suggesting a strong connection between hoarding strategy and pilfering ability.

关键词： Caching pilfering Hoarding strategy Sympatric rodents Coexistence

Minimizing Risks of Antimicrobial Resistance Development in the Environment from a Public One Health Perspective

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China CDC weekly 2022年第49期4卷 1105-1109页

作者： Yu Zhang Timothy R.Walsh Yang Wang Jianzhong Shen Min Yang State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina Ineos Oxford Institute of Antimicrobial Research Department of BiologyUniversity of OxfordOxfordUK Beijing Key Laboratory of Detection technology for Animal-Derived food Safety College of Veterinary MedicineChina Agricultural UniversityBeijingChina University of Chinese Academy of Sciences BeijingChina.

Antimicrobial resistance(AMR)is a globally recognized crisis with meaningful engagement across humans,animals,and the environment as in the One Health *** environment is the potential source,reservoir,and transmission route of AMR,and it plays a key role in AMR development from the One Health *** farming,hospitals,and the pharmaceutical industry are identified as the main emission sources in the *** emissions and determining antimicrobial emission limits are priorities in the containment of environmental AMR *** the perspectives of environmental management and environmental engineering,some important actions to minimize risks of AMR development are summarized,including the recent progress in enhanced hydrolysis pre-treatment technology to control the development of antibiotic resistance genes(ARGs)during biological wastewater *** is desirable to establish a holistic framework to coordinate international actions on the containment of environmental AMR *** establish a community with a shared future for humanity,China should and could play an important role in international cooperation to cope with AMR challenges.

关键词： reservoir summarized globally

供氧策略对光合细菌污水资源化系统微生物群落及碳氮代谢影响

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环境科学学报 2024年第1期44卷 193-203页

作者：何仕超卢海凤张光明李保明高风正司哺春蒋伟忠中国农业大学三亚研究院三亚572025 中国农业大学水利与土木工程学院北京100083 农业农村部设施农业工程重点实验室北京100083 河北工业大学能源与环境学院天津300401 ETH Zürich Sustainable Food Processing LaboratoryZürich 8092 ETH Zürich Laboratory of Nutrition and Metabolic EpigeneticsSchwerzenbach 8603 海南热带海洋学院食品科学与工程学院三亚572022

为应对连续供氧会削弱光合细菌(Photosynthetic Bacteria,PSB)污水资源化系统中PSB菌群优势的问题,基于PSB特殊的光合磷酸化与氧化磷酸化代谢互作机制,提出了12h-厌氧/12h-供氧的间歇供氧(A/M-O)策略,并与连续厌氧(A-O)、连续供氧(M-O)... 详细信息

为应对连续供氧会削弱光合细菌(Photosynthetic Bacteria,PSB)污水资源化系统中PSB菌群优势的问题,基于PSB特殊的光合磷酸化与氧化磷酸化代谢互作机制,提出了12h-厌氧/12h-供氧的间歇供氧(A/M-O)策略,并与连续厌氧(A-O)、连续供氧(M-O)策略下PSB污水资源化效果、微生物群落结构和功能特点进行了对比,解析了供氧策略影响PSB碳氮代谢的机制.结果表明,A/M-O策略可显著提高PSB生物量(2419.3 mg·L^(-1))和蛋白质浓度(1411.5 mg·L^(-1)),较A-O和M-O组的生物量浓度分别提高了76.4%和26.4%,蛋白质浓度分别提高了67.4%和40.5%.M-O和A/M-O策略显著提高了化学需氧量(Chemical oxygen demand,COD)和氨氮(Ammonia,NH_(4)^(+)-N)去除率及蛋白质含量.上述3种供氧策略下系统菌群结构差异显著,系统中唯一的PSB菌种Ectothiorhodospira在A/M-O组中相对丰度提高至78.0%,分别比A-O和M-O组高1.5和4.4倍.M-O和A/M-O组的Ace和Chao指数、微生物网络的“节点”和“连接”数及正相关比例均高于A-O组,表明M-O和A/M-O策略有利于维持微生物群落结构和功能的稳定.Tax4Fun功能预测表明,A/M-O策略通过促进EMP途径和TCA循环的关键酶编码基因的相对丰度来提高生物量和蛋白质浓度;M-O和A/M-O策略通过提高碳代谢、氮代谢关键酶编码基因的相对丰度并改变系统的氧化还原电位(Oxidative redox potential,ORP)来提高系统的COD和NH_(4)^(+)-N去除率及蛋白质含量,强化了系统中氮代谢功能.研究结果创新了PSB污水资源化系统中保持PSB菌群占比优势、提升微生物群落结构和功能稳定性的方法.

关键词：供氧策略光合细菌污水资源化菌群优势碳氮代谢

Coagulant compounds in Rubia cordifolia L.

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Journal of Future foods 2023年第3期3卷 220-224页

作者： Peiqing Wang Jiajia Wang Fatma MEl-Demerdash Jinfeng Wei Huaihe Hospital Henan UniversityKaifeng 475004China National R&D Center for Edible Fungus Processing technology Henan UniversityKaifeng 475004China Department of Environmental Studies Institute of Graduate Studies and ResearchAlexandria UniversityAlexandria 21526Egypt Joint International Research Laboratory of food&Medicine Resource Function Henan ProvinceKaifeng 475004China Henan Kaifeng College of Science technology and Communication Kaifeng 475004China

Rubia cordifolia *** to Rubiaceae *** medicinal parts are mainly root and rhizome,which is included in the Chinese Pharmacopoeia(2020),has the effect of cooling blood and stopping bleeding,removing stasis and channeling ***,studies on the hemostatic effect of *** mainly focus on extracts,and the hemostatic components are less ***,chemical constituents and coagulant activity of *** were investigated in this *** compounds were isolated and identified from ***,including 1-hydroxy-2-methyl-9,10-anthraquinone(1),1,4-dihydroxy-2-methylanthraquinone(2),palmitic acid(3),tricosanoic acid(4),ω-hydroxypachybasin(5),ursolic acid(6),oleanolic acid(7),rubilactone(8),β-sitosterol(9),cordifoliol(10),1,3-dihydroxy-2-ethoxymethyl-9,10-anthraquinone(11).ω-Hydroxypachybasin was isolated from Rubia genus for the first time.1,4-Dihydroxy-2-methylanthraquinone,rubilactone and 1,3-dihydroxy-2-ethoxymethyl-9,10-anthraquinone could remarkably shorten the plasma calcium coating time in vitro,thus indicating that they had procoagulant activity.

关键词： Rubia cordifolia L. Chemical constituents Coagulant activity

Estimation of heritabilities of disease resistance to Edwardsiella tarda and genetic correlations between resistance and growth traits in Chinese tongue sole (Cynoglossus semilaevis)

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Aquaculture and Fisheries 2020年第6期5卷 289-293页

作者： Ming Li Yingming Yang Weiwei Zheng Zhongming Li Jiayu Cheng Yangzhen Li College of Fisheries and Life Science Shanghai Ocean UniversityShanghai201306China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences(CAFS)Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdao266071China Weifang Sanxin Aquatic technology Development Co. Ltd.Weifang261311China Tangshan Weizhuo Aquaculture Co. Ltd.Tangshan063200China

Chinese tongue sole(Cynoglossus semilaevis)is an important marine flatfish which is widely cultured in coastal areas in *** bacterial diseases such as Edwardsiella tarda imposed serious threats to this species.A potential strategy to prevent this disease is to select resistance *** aim of the present work was to estimate the genetic variation of disease resistance to *** and to evaluate the genetic correlations between resistance traits and growth *** types of models were fitted by using different trait definitions(binary,continuous and categorical).After a 9-day challenge test,the overall survival was 75.4%(ranging from 6.4%to 100%in families),and at test day 6,the overall survival was 50.7%.We set test day 6 and 9 as cut-off point times *** heritabilities of survival traits were ranging from 0.10 to *** higher heritability values were obtained at day 6 than at day 9,regardless of which model or trait definition used(except Trait 2 in LIN).The genetic correlations between disease resistance traits and growth traits(*** weight and total length)were low and not significant from zero(−0.12–0.24).There is a substantial re-ranking of families when defined resistance as categorical and continuous traits compared to binary *** results confirm the existence of genetic variation for resistance against *** and weak genetic correlations indicate that joint genetic improvement of *** resistance and growth is scarcely available.

关键词： Heritability Genetic parameter Disease resistance Genetic correlation Edwardsiella tarda Cynoglossus semilaevis