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Genome-wide association studies for inflorescence type and remontancy in Hydrangea macrophylla

Horticulture research 2020年第1期7卷 2477-2485页

作者： Xingbo Wu Lisa W.Alexander Oak Ridge Institute of Science and Technology Otis L.Floyd Nursery Research Center472 Cadillac LaneMcMinnvilleTNUSA U.S.Department of Agriculture Agricultural Research ServiceU.S.National ArboretumFloral and Nursery Plants Research UnitOtis L.Floyd Nursery Research Center472 Cadillac LaneMcMinnvilleTNUSA

Inflorescence type and remontancy are two valuable traits in bigleaf hydrangea(Hydrangea macrophylla L.)and both are recessively inherited.Molecular marker-assisted selection(MAS)can greatly reduce the time necessary to breed cultivars with desired traits.In this study,a genome-wide association study(GWAS)using 5803 single-nucleotide polymorphisms(SNPs)was performed using a panel of 82 bigleaf hydrangea cultivars.One SNP locus(Hy_CAPS_Inflo)associated with inflorescence type was identified with general linear model(GLM)and mixed linear model(MLM)methods that explained 65.5%and 36.1%of the phenotypic variations,respectively.Twenty-three SNPs associated with remontancy were detected in GLM whereas no SNP was detected in MLM.The SNP locus(Hy_CAPS_Inflo)was converted to a cleaved amplified polymorphic sequence(CAPS)marker that showed absolute identification accuracy(100%)of inflorescence type in a validation panel consisting of eighteen H.macrophylla cultivars.The SNP was investigated in 341 F1 progenies using genotyping by sequencing(GBS)and co-segregated with inflorescence type(χ^(2)=0.12;P=0.73).The SNP was subsequently used for breeding selection using kompetitive allele specific PCR(KASP)technology.Future directions for the use of genomics and MAS in hydrangea breeding improvement are discussed.The results presented in this study provide insights for further research on understanding genetic mechanisms behind inflorescence type and remontancy in H.macrophylla.The CAPS and KASP markers developed here will be immediately useful for applying MAS to accelerate breeding improvement in hydrangea.

关键词： breeding cultivar CAPS

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Two haplotype-resolved genomes reveal important flower traits in bigleaf hydrangea(Hydrangea macrophylla)and insights into Asterid evolution

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Horticulture research 2023年第12期10卷 45-57页

作者： Xingbo Wu Sheron A.Simpson Ramey C.Youngblood Xiaofen FLiu Brian E.Scheffler Timothy A.Rinehart Lisa W.Alexander Amanda M.Hulse-Kemp Department of Environmental Horticulture Tropical Research and Education CenterUniversity of FloridaHomesteadFL 33031United States Genomics and Bioinformatics research unit USDA-ARSRaleighNC 27695United States Genomics and Bioinformatics research unit USDA-ARSStonevilleMS 38776United States Institute for Genomics Biocomputing and BiotechnologyMississippi State UniversityStarkvilleMS 39762United States Crop Production and Protection USDA-ARSBeltsvilleMD 20705United States floral and nursery plants research unit U.S.National ArboretumUSDA-ARSMcMinnvilleTN 37110United States Department of Crop and Soil Sciences North Carolina State UniversityRaleighNC 27695United States

The Hydrangea genus belongs to the Hydrangeaceae family,in the Cornales order of flowering plants,which early diverged among the Asterids,and includes several species that are commonly used ornamental plants.Of them,Hydrangea macrophylla is one of the most valuable species in the nursery trade,yet few genomic resources are available for this crop or closely related Asterid species.Two high-quality haplotype-resolved reference genomes of hydrangea cultivars‘Veitchii’and‘Endless Summer’[highest quality at 2.22 gigabase pairs(Gb),396 contigs,N5022.8 megabase pairs(Mb)]were assembled and scaffolded into the expected 18 pseudochromosomes.Utilizing the newly developed high-quality reference genomes along with high-quality genomes of other related flowering plants,nuclear data were found to support a single divergence point in the Asterids clade where both the Cornales and Ericales diverged from the euasterids.Genetic mapping with an F1 hybrid population demonstrated the power of linkage mapping combined with the new genomic resources to identify the gene for inflorescence shape,CYP78A5 located on chromosome 4,and a novel gene,BAM3 located on chromosome 17,for causing double flower.Resources developed in this study will not only help to accelerate hydrangea genetic improvement but also contribute to understanding the largest group of flowering plants,the Asterids.

关键词： Hydra traits resolved

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New opportunities for using WUS/BBM and GRF-GIF genes to enhance genetic transformation of ornamental plants

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Ornamental Plant research 2022年第1期2卷 32-38页

作者： Hui Duan Nathan A.Maren Thomas G.Ranney Wusheng Liu USDA-ARS U.S.National ArboretumFloral and Nursery Plants Research UnitBeltsville Agricultural Research Center(BARC)-WestBeltsvilleMD 20705USA Department of Horticultural Science North Carolina State UniversityRaleighNC 27607USA Mountain Crop Improvement Lab Department of Horticultural ScienceMountain Horticultural Crops Research and Extension CenterNorth Carolina State UniversityMills RiverNC 28759USA

Broad application of plant transformation remains challenging because the efficiency of plant regeneration and regeneration-based transformation in many plant species is extremely low.Many species and genotypes are not responsive to traditional hormone-based regeneration systems.This regeneration recalcitrance hampers the application of many technologies such as micropropagation,transgenic breeding,and gene editing in various plant species,including ornamental flowers,shrubs,and trees.Various developmental genes have long been studied for their ability to improve plant meristematic induction and regeneration.Lately,it was demonstrated that the combined and refined expression of morphogenic regulator genes WUSCHEL and BABY BOOM could alleviate their pleiotropic effects and permit transformation in recalcitrant monocots.Moreover,ectopic expression of plant growth-regulating factors(GRFs)alone or in combination with GRF-interacting factors(GIFs)improved the regeneration and transformation of dicot and monocot species.Fine-tuning the expression of these genes provides new opportunities to improve transformation efficiencies and facilitate the application of new breeding technologies in ornamental plants.

关键词： transformation GRF breeding

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Unraveling the regulatory network of flower coloration in soybean:Insights into roles of GmMYBA3 and GmMYBR1

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The Crop Journal 2024年第2期12卷 443-455页

作者： Ruifang Gao Yueqing Li Yanan Wang Xiaotong Shan Siqi Yang Ye Zhang Saiyu Ma Chunbao Zhang Jianchun Qin Li Wang Xiang Gao College of Plant Science Jilin UniversityChangchun 130062JilinChina Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics&Cytology Northeast Normal UniversityChangchun 130024JilinChina Soybean research Institute National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033JilinChina

Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in other processes remain largely unresolved in soybeans(Glycine max).In this study,we investigated the genetic components and mechanisms governing anthocyanin biosynthesis in soybean flowers.Molecular and genetic studies have characterized two antagonistic regulators,the positive activator GmMYBA3 and the negative repressor GmMYBR1,that modulate the gene expression of anthocyanin biosynthesis in soybean flowers.Further findings revealed a regulatory interplay between GmMYBA3 and GmMYBR1 bridged by GmTT8a,highlighting the complexity of anthocyanin regulation in different soybean organs.Exploration of additional soybean cultivars demonstrated the universality of GmMYBA3 and GmMYBR1 in regulating floral anthocyanin biosynthesis-related genes,with GmF3’5’H identified as a crucial determinant of white flower color.This study provides a molecular mechanism underlying soybean flower color determination,paving the way for the molecular modification of soybean flowers to probably enhance their resistance to abiotic stresses and attractiveness to pollinators.

关键词： Anthocyanin Flower color determination MBW complex MYB repressor Pleiotropic regulator

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Identification of Tsuga Germplasm by Morphological Characters and RAPD Markers

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应用与环境生物学报 2007年第2期13卷 145-151页

作者： ROH Mark S DE BENEDETTI Laura JOUNG Young Hee LEE Nam Sook US Department of Agriculture Agricultural Research ServiceNational ArboretumFloral and Nursery Plants Research UnitBeltsvilleMD 20705USA Istituto Sperimentale per la Floricoltura SanremoImperiaItaly Chonnam National University School of Biological Sciences and TechnologyGwangjuKorea Ewha Womans University Department of Life SciencesSeoulKorea

Germplasm collection is important to preserve and maximize genetic diversity for germplasm conservation.Tsuga dumosa(D.Don)Eichler in Engler & Prantl.and T.chinensis var.forrestii(Downie)Silba germplasm was collected from three localities in China:Mt.Yulong,Wenfeng Temple and Mt.Dishiergu,Yunnan Province.Accessions were identified based on morphological characters and RAPD markers.The shapes of the apices and margins of needles were examined,and the length and width of needles,cones and seeds from accessions of mature plants were used to compare the morphological differences and to identify the germplasm.Molecular markers generated by randomly amplified polymorphic DNA(RAPD)were also used to characterize the taxa.Although the clustering based on RAPD markers was inconsistent with the morphological characters of the needles,based on the overall morphological characters and on RAPD markers,the accessions from Mt.Yulong and Wenfeng Temple were identified as T.chinensis var.forrestii,and those from Mt.Dishiergu identified as T.dumosa.Taxonomic identification of the accessions was made based on morphology and by RAPD markers concurred.The results indicate that the shapes of the apices and margins of needles particularly from young plants could not be used as a possible key to identify T.dumosa and T.chinensis var.forrestii.Fig 6,Tab 3,Ref

关键词：形态特征 RAPD分子标记种质鉴定云南铁杉华铁杉

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Engineered Cleistogamy in Camelina sativa for bioconfinement

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Horticulture research 2023年第2期10卷 275-285页

作者： Debao Huang Liwei Gao Jeremy McAdams Fangzhou Zhao Hongyan Lu Yonghui Wu Jeremy Martin Sherif MSherif Jayasankar Subramanian Hui Duan Wusheng Liu Department of Horticultural Science North Carolina State UniversityRaleighNC 27607USA College of Life Sciences Ganzhou Normal UniversityGanzhouJiangxi 341000China National Center for Soybean Improvement State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingJiangsu 210095China College of Food Science and Engineering Wuhan Polytechnic UniversityWuhanHubei 430048China Sandhills research Station North Carolina State UniversityJackson SpringsNC 27281USA Vineland research Station Department of Plant AgricultureUniversity of GuelphVinland StationON LOR 2E0Canada Alson H.Smith Jr.Agricultural research and Extension Center School of Plant and Environmental SciencesVirginia TechWinchesterVA 22602USA USDA-ARS U.S.National ArboretumFloral and Nursery Plants Research UnitBeltsville Agricultural Research Center(BARC)-WestBeltsvilleMD 20705USA

Camelina sativa is a self-pollinating and facultative outcrossing oilseed crop.Genetic engineering has been used to improve camelina yield potential for altered fatty acid composition,modified protein profiles,improved seed and oil yield,and enhanced drought resistance.The deployment of transgenic camelina in the field posits high risks related to the introgression of transgenes into nontransgenic camelina and wild relatives.Thus,effective bioconfinement strategies need to be developed to prevent pollen-mediated gene f low(PMGF)from transgenic camelina.In the present study,we overexpressed the cleistogamy(i.e.f loral petal non-openness)-inducing PpJAZ1 gene from peach in transgenic camelina.Transgenic camelina overexpressing PpJAZ1 showed three levels of cleistogamy,affected pollen germination rates after anthesis but not during anthesis,and caused a minor silicle abortion only on the main branches.We also conducted field trials to examine the effects of the overexpressed PpJAZ1 on PMGF in the field,and found that the overexpressed PpJAZ1 dramatically inhibited PMGF from transgenic camelina to non-transgenic camelina under the field conditions.Thus,the engineered cleistogamy using the overexpressed PpJAZ1 is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina,and could be used for bioconfinement in other dicot species.

关键词： drought expressing limit

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Volatile metabolome and transcriptome reveal fragrance release rhythm and molecular mechanisms of Rosa yangii

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Journal of Integrative Agriculture 2023年第7期22卷 2111-2125页

作者： ZHOU Li-jun HUANG Run-huan LIU Ting-han LIU Wei-chao CHEN Yun-yi LÜPei-feng LUO Le PAN Hui-tang YU Chao ZHANG Qi-xiang Beijing Key Laboratory of Ornamental plants Germplasm Innovation&Molecular Breeding/National Engineering research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental plants of Ministry of Education/School of Landscape Architecture Beijing Forestry UniversityBeijing 100083P.R.China

Rose is a highly significant ornamental plant with substantial edible and medicinal value,cultivated worldwide primarily for perfume production.Recently,Rosa yangii,a new species found in northwestern Yunnan,China,has drawn attention due to its strong sweet scented flowers.In this study,the floral components of R.yangii were extracted at different flowering stages using solid phase micro extraction(SPME)and analyzed through gas chromatography-mass spectrometry(GC-MS).A total of 131 volatile organic compounds(VOCs)were detected from R.yangii,including 69 odor compounds.The production and release of floral VOCs were the highest during the initial-open stage,making it the most suitable time for harvesting as a significant number of floral components were synthesized and preserved.The analysis of the odor activity values(OAV)highlighted several key aromatic ingredients of R.yangii,such as eugenol,methyleugenol,benzeneacetaldehyde and phenylethylalcohol,heptanal,decanal,(E)-2-hexen-1-yl acetate,caryophyllene,and others.Metabolome and time-order gene co-expression networks(TO-GCN)revealed that VOCs and benzenoids/phenylpropanoids,along with associated genes,played a pivotal role in the overall floral regulatory network of R.yangii.MYB and bHLH were identified as the essential regulatory factors governing the regulation of eugenol synthase(EGS)and isoeugenol synthase(IGS),consequently influencing the sweet scent of R.yangii.The findings of this study provide a scientific foundation for enhancing fragrance through molecular breeding of ornamental plants.Furthermore,the study facilitated the development and utilization of this new plant’s essential oil material in various industries,including food storage,aromatherapy,cosmetic,and perfumery.

关键词： rose sweet fragrance SPME-GC-MS aroma VOCs TO-GCN

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Positive regulatory role of R2R3 MYBs in terpene biosynthesis in Lilium‘Siberia’

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Horticultural Plant Journal 2023年第5期9卷 1024-1038页

作者： Yanhong Guo Ziyu Guo Jian Zhong Yilin Liang Yuan Feng Peng Zhang Qixiang Zhang Ming Sun State Key Laboratory of Efficient Production of Forest Resources Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of EducationSchool of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China

Terpenoids are the main components contributing to the fragrance of Lilium‘Siberia’,and LiTPS2 plays a critical role in the biosynthesis of monoterpenoids.Although the major terpene synthases in Lilium‘Siberia’have been identified,how these TPS genes are transcriptionally regulated remains elusive in this distinguished flower.This study aimed to identify transcription factors that regulate the terpene synthesis in Lilium,and disclose the related underlying transcriptional regulation mechanism.In this study,we identified three R2R3-MYB TFs—LiMYB1,LiMYB305 and LiMYB330,which were involved in regulating the biosynthesis of terpenes in Lilium‘Siberia’.Quantitative real-time PCR showed spatial and temporal expression patterns consistent with the emission patterns of terpene compounds.When LiMYB1,LiMYB305 and LiMYB330were overexpressed in flowers,the release of some main monoterpenes,such as linalool and ocimene,as well as the expression of TPS genes,especially for LiTPS2,were enhanced.A virus-induced gene silencing(VIGS)assay showed that silencing these three LiMYBs decreased the level of monoterpenes by down-regulating the expression of the TPS genes.The yeast one-hybrid and transient expression assays indicated that all three LiMYBs could bind to and activate the promoter of LiTPS2.Moreover,the yeast two-hybrid assay verified that LiMYB1 could interact with LiMYB308 and LiMYB330,indicating their synergistic roles in the regulation of floral terpene biosynthesis.In general,these results indicated that LiMYB1,LiMYB305,and LiMYB330 might play essential roles in terpene biosynthesis in Lilium and would provide a new perspective for the transcriptional regulation of volatile terpenes in flowers.

关键词： Lilium R2R3-MYB Expression analysis Terpenes Regulation

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A hybrid swarm population of Pinus densiflora × P. sylvestris inferred from sequence analysis of chloroplast DNA and morphological characters

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Journal of Forestry research 2013年第1期24卷 53-60页

作者： Young Hee Joung Jerry L. Hill Jung Oh Hyun Ding Mu Juchun Luo Do Hyung Lee Takayuki Kawahara Jeung Keun Suh Mark S. Roh Chonnam National University School of Biological Sciences & TechnologyGwangjuKorea US Department of Agriculture Forest ServiceDorena Genetic Resource CenterWashington State UniversityPullmanWA99164USA Seoul National University College of Agriculture and Life SciencesSeoulKorea Chinese Academy of Agricultural Science Beijing Forestry University College of Resources and Environment Yeungnam University College of Natural ResourcesDepartment of Forest ResourcesGyeongsanKorea Hokkaido research Center Forestry and Forest Products Research InstituteSapporoJapan. Dankook University School of Bio-Resource ScienceDepartment of Environment HorticultureCheonanKorea. US Department of Agriculture Agricultural Research ServiceUS National ArboretumFloral and Nursery Plants Research UnitBeltsvilleMD 20705USA.

To confirm a hybrid swarm population of Pinus densiflora × P. sylvestris in Jilin, China, we used needles and seeds from P. densiflora, P. sylvestris, and P. densiflora × P. sylvestris collected from natural stands or experimental stations to study whether shoot apex morphology of 4-year old seedlings can be correlated with the sequence of a chloroplast DNA simple sequence repeat marker （cpDNA SSRs）. Total genomic DNA was extracted and subjected to sequence analysis of the pine cpDNA SSR marker Pt15169. Results show that morphological characters from 4-year old seedlings did not correlate with sequence variants of this marker. Marker haplotypes from all P. sylvestris trees had a CTAT element that was absent from all sampled P. densiflora trees. However, both haplotype classes involving this insertion/deletion element were found in a P. densiflora × P. sylvestris population and its seedling progeny. It was concluded that the P. densiflora × P. sylvestris accessions sampled from Jilin, China resulted from bi-directional crosses, as evidenced by both species’ cpDNA haplotypes within the hybrid swarm population.

关键词： P. sylvestris var. sylvestriformis chloroplast DNA simplesequence repeat sequencing hybrid swarm population

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Substrate,Hormone,Winnowing,and Stratification Influence the Seed Germination of Ilex asprella(Hook.et Arn.)Champ.ex Benth

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2105-2116页

作者： Yuquan Huang Fan Wei QingMa Yang Lin Jinpeng Huang Yanxia Zhu Danfeng Tang Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement Guangxi Botanical Garden of Medicinal PlantsNanning530000China research and Development Center China Resources Sanjiu Medical&Pharmaceutical Co.Ltd.Shenzhen518000China

Ilex asprella(Hook.et Arn.)Champ.ex Benth is one of the most important traditional Chinese medicines in southern China.The seeds of Ilex asprella usually have extremely low germination due to their dormancy characteristics,which severely impacts the efficiency of seedling raising and increases labor costs.In this study,to improve the seed germination of I.asprella,the effects of germination substrate,hormone,winnowing,and stratification treatments on the seed germination of I.asprella were investigated.The results of the germination matrix showed that the highest germination percentage of 45.2%was achieved under the 20℃/10℃day/night temperature and vermiculite germination medium conditions.The results of hormone treatments revealed that 100–400 mg/L of gibberellin(GA)and 50–100 mg/L of salicylic acid(SA)were found to be effective in releasing the dormancy of I.asprella seeds.Moreover,winnowing could effectively eliminate unsaturated seeds and impurities,thus improving the seed germination of I.asprella.Furthermore,warm temperature(15℃)stratification could expand the temperature range of I.asprella’s seed germination,which was beneficial for seed germination of I.asprella and for seed nursery at room temperature in production practice.The present study obtained a method to break dormancy and increase seed germination in I.asprella,thereby forming a groundwork for improving the efficiency of large-scale planting of I.asprella.

关键词： Ilex asprella seed germination substrate winnowing hormone stratification

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