检索结果-南通市图书馆

Noncovalent reversible binding-enabled facile fabrication of leak-free PDMS microfluidic devices without plasma treatment for convenient cell loading and retrieval

引用

Bioactive Materials 2022年第10期7卷 346-358页

作者： Bin Jiang Alisa White Wenquan Ou Sarah Van Belleghem Samantha Stewart James G.Shamul Shaik O.Rahaman John P.Fisher Xiaoming He fischell department of bioengineering University of MarylandCollege ParkMD20742USA department of Nutrition and Food Science University of MarylandCollege ParkMD20742USA Marlene and Stewart Greenebaum Comprehensive Cancer Center University of MarylandBaltimoreMD21201USA

The conventional approach for fabricating polydimethylsiloxane(PDMS)microfluidic devices is a lengthy and inconvenient procedure and may require a clean-room microfabrication facility often not readily ***,living cells can’t survive the oxygen-plasma and high-temperature-baking treatments required for covalent bonding to assemble multiple PDMS parts into a leak-free device,and it is difficult to disassemble the devices because of the irreversible covalent *** a result,seeding/loading cells into and retrieving cells from the devices are ***,we discovered that decreasing the curing agent for crosslinking the PDMS prepolymer increases the noncovalent binding energy of the resultant PDMS surfaces without plasma or any other *** enables convenient fabrication of leak-free microfluidic devices by noncovalent binding for various biomedical applications that require high pressure/flow rates and/or long-term cell culture,by simply hand-pressing the PDMS parts without plasma or any other treatment to bind/*** this method,multiple types of cells can be conveniently loaded into specific areas of the PDMS parts before assembly and due to the reversible nature of the noncovalent bonding,the assembled device can be easily disassembled by hand peeling for retrieving *** with 3D printers that are widely available for making masters to eliminate the need of photolithography,this facile yet rigorous fabrication approach is much faster and more convenient for making PDMS microfluidic devices than the conventional oxygen plasma-baking-based irreversible covalent bonding method.

关键词： Binding energy 3D printing Soft lithography Microfluidics Polydimethylsiloxane

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Trehalose:A sugar molecule involved in temperature stress management in plants

引用

The Crop Journal 2024年第1期12卷 1-16页

作者： Ali Raza Savita Bhardwaj md Atikur Rahman Pedro García-Caparrós Madiha Habib Faisal Saeed Sidra Charagh Christine H.Foyer Kadambot H.M.Siddique Rajeev K.Varshney college of Agriculture Fujian Agriculture and Forestry University(FAFU)Fuzhou 350002FujianChina department of Botany School of Bioengineering and BiosciencesLovely Professional UniversityPhagwara 144411India department of Botany MCM DAV CollegeKangraHimachal Pradesh 176001India Grassland and Forage Division National Institute of Animal ScienceRural Development AdministrationCheonan 31000Republic of Korea Agronomy department of Superior School Engineering University of AlmeríaAlmeríaSpain National Institute for Genomics and Advanced Biotechnology National Agricultural Research CentrePark Rd.Islamabad 45500Pakistan Agriculture Health and Environment DepartmentNatural Resources InstituteFaculty of Engineering&ScienceUniversity of GreenwichKent ME44TBUK State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhouZhejiangChina School of Biosciences College of Life and Environmental SciencesUniversity of BirminghamEdgbaston B152TTUK The UWA Institute of Agriculture The University of Western AustraliaCrawleyPerth 6009Australia WA State Agricultural Biotechnology Centre Centre for Crop and Food InnovationFood Futures InstituteMurdoch UniversityMurdochWA 6150Australia

Trehalose(Tre)is a non-reducing disaccharide found in many species,including bacteria,fungi,invertebrates,yeast,and even plants,where it acts as an osmoprotectant,energy source,or protein/membrane *** relatively small amounts in plants,Tre concentrations increase following exposure to abiotic ***-6-phosphate,a precursor of Tre,has regulatory functions in sugar metabolism,crop production,and stress *** the various abiotic stresses,temperature extremes(heat or cold stress)are anticipated to impact crop production worldwide due to ongoing climate *** small amounts of Tre can mitigate negative physiological,metabolic,and molecular responses triggered by temperature *** also interacts with other sugars,osmoprotectants,amino acids,and phytohormones to regulate metabolic reprogramming that underpins temperature stress *** plants expressing Tre-synthesis genes accumulate Tre and show improved stress ***-wide studies of Tre-encoding genes suggest roles in plant growth,development,and stress *** review discusses the functions of Tre in mitigating temperature stress—highlighting genetic engineering approaches to modify Tre metabolism,crosstalk,and interactions with other molecules—and in-silico approaches for identifying novel Tre-encoding genes in diverse plant *** consider how this knowledge can be used to develop temperature-resilient crops essential for sustainable agriculture.

关键词： Abiotic stress Gene expression Genetic engineering Osmolyte Trehalose-6-phosphate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rock inhibitor may compromise human induced pluripotent stem cells for cardiac differentiation in 3D

引用

Bioactive Materials 2022年第3期7卷 508-522页

作者： Bin Jiang Wenquan Ou James G.Shamul Hao Chen Sarah Van Belleghem Samantha Stewart Zhenguo Liu John P.Fisher Xiaoming He fischell department of bioengineering University of MarylandCollege ParkMD20742United States Division of Cardiovascular Medicine University of Missouri School of MedicineColumbiaMO65212United States Marlene and Stewart Greenebaum Comprehensive Cancer Center University of MarylandBaltimoreMD21201United States

Cardiomyocytes differentiated from human induced pluripotent stem cells(iPSCs)are valuable for the understanding/treatment of the deadly heart diseases and their drug ***,the very much needed homogeneous 3D cardiac differentiation of human iPSCs is still ***,it is discovered surprisingly that Rock inhibitor(RI),used ubiquitously to improve the survival/yield of human iPSCs,induces early gastrulation-like change to human iPSCs in 3D culture and may cause their heterogeneous differentiation into all the three germ layers(i.e.,ectoderm,mesoderm,and endoderm)at the commonly used concentration(10μM).This greatly compromises the capacity of human iPSCs for homogeneous 3D cardiac *** reducing the RI to 1μM for 3D culture,the human iPSCs retain high pluripotency/quality in inner cell mass-like solid 3D ***,the beating efficiency of 3D cardiac differentiation can be improved to more than 95%in~7 days(compared to less than~50%in 14 days for the 10μM RI condition).Furthermore,the outset beating time(OBT)of all resultant cardiac spheroids(CSs)is synchronized within only 1 day and they form a synchronously beating 3D construct after 5-day culture in gelatin methacrylol(GelMA)hydrogel,showing high homogeneity(in terms of the OBT)in functional maturity of the ***,the resultant cardiomyocytes are of high quality with key functional ultrastructures and highly responsive to cardiac *** discoveries may greatly facilitate the utilization of human iPSCs for understanding and treating heart diseases.

关键词： Episomal iPSC Cardiomyocyte Spheroid GelMA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Axial strain enhances osteotomy repair with a concomitant increase in connexin43 expression

引用

Bone Research 2015年第2期3卷 83-91页

作者： Rishi R Gupta Hyunchul Kim Yu-Kwan Chan Carla Hebert Leah Gitajn David J Yoo Robert V O’Toole Adam H Hsieh Joseph P Stains department of Orthopaedics University of Maryland fischell department of bioengineering University of MarylandCollege Park

The mechanical environment is known to influence fracture healing. We speculated that connexin43 （Cx43） gap junctions, which impact skeletal homeostasis, fracture healing and the osteogenic response to mechanical load, may play a role in mediating the response of the healing bone to mechanical strain. Here, we used an established rat fracture model, which uses a 2 mm osteotomy gap stabilized by an external fixator, to examine the impact of various cyclical axial loading protocols （2%, 10%, and 30% strain） on osteotomy healing. We examined the presence of Cx43 in the osteotomy-healing environment and assessed how mechanical strain modulates Cx43 expression patterns in the callus. We demonstrated that increased cyclical axial strain results in increased radiographic and histologic bone formation. In addition, we show by immunohistochemistry that Cx43 is abundantly expressed in the healing callus, with the expression most robust in samples exposed to increased cyclical axial strain. These data are consistent with the concept that an increase in Cx43 expression by mechanical load may be part of the mechanisms by which mechanical forces enhances fracture healing.

关键词： EXPERIMENTAL TIBIAL FRACTURES TISSUE DIFFERENTIATION MECHANICAL-STRESS COMPRESSION OSTEOBLAST MICE

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Freestanding region-responsive bilayer for functional packaging of ingestible devices

引用

微系统与纳米工程(英文) 2023年第3期9卷 285-295页

作者： Michael A.Straker Joshua A.Levy Justin M.Stine Vivian Borbash Luke A.Beardslee Reza Ghodssi fischell department of bioengineering University of MarylandCollege ParkMD 20742USA Institute for Systems Research University of MarylandCollege ParkMD 20740USA Robert E.fischell Institute for Biomedical Devices University of MarylandCollege ParkMD 20850USA department of Material Science and Engineering University of MarylandCollege ParkMD 20740USA department of Electrical and Computer Engineering University of MarylandCollege ParkMD 20742USA

Ingestible capsules have the potential to become an attractive alternative to traditional means of treating and detecting gastrointestinal(GI)*** device complexity increases,so too does the demand for more effective capsule packaging technologies to elegantly target specific GI *** pH-responsive coatings have been traditionally used for the passive targeting of specific GI regions,their application is limited due to the geometric restrictions imposed by standard coating ***,pan,and spray coating methods only enable the protection of microscale unsupported openings against the harsh GI ***,some emerging technologies have millimeter-scale components for performing functions such as sensing and drug *** this end,we present the freestanding region-responsive bilayer(FRRB),a packaging technology for ingestible capsules that can be readily applied for various functional ingestible capsule *** bilayer is composed of rigid polyethylene glycol(PEG)under a flexible pH-responsive Eudragit®FL 30 D 55,which protects the contents of the capsule until it arrives in the targeted intestinal *** FRRB can be fabricated in a multitude of shapes that facilitate various functional packaging mechanisms,some of which are demonstrated *** this paper,we characterize and validate the use of this technology in a simulated intestinal environment,confirming that the FRRB can be tuned for small intestinal *** also show a case example where the FRRB is used to protect and expose a thermomechanical actuator for targeted drug delivery.

关键词： coating packaging attractive

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

3D-Printed electrochemical sensor-integrated transwell systems

引用

Microsystems & Nanoengineering 2020年第1期6卷 357-368页

作者： Pradeep Ramiah Rajasekaran Ashley Augustiny Chapin David N.Quan Jens Herberholz William E.Bentley Reza Ghodssi Institute for Systems Research University of MarylandCollege ParkMDUSA fischell department of bioengineering University of MarylandCollege ParkMDUSA department of Psychology and Neuroscience and Cognitive Science Program University of MarylandCollege ParkMDUSA Institute for Bioscience and Biotechnology Research University of MarylandCollege ParkMDUSA Robert E.fischell Institute for Biomedical Devices University of MarylandCollege ParkMDUSA department of Electrical and Computer Engineering University of MarylandCollege ParkMDUSA

This work presents a 3D-printed,modular,electrochemical sensor-integrated transwell system for monitoring cellular and molecular events in situ without sample extraction or microfluidics-assisted downstream *** additive manufacturing techniques such as 3D printing,shadow masking,and molding are used to fabricate this modular system,which is autoclavable,biocompatible,and designed to operate following standard operating protocols(SOPs)of cellular *** to the platform is a flexible porous membrane,which is used as a cell culture substrate similarly to a commercial transwell *** electrochemical sensors fabricated on the membrane allow direct access to cells and their products.A pair of gold electrodes on the top side of the membrane measures impedance over the course of cell attachment and growth,characterized by an exponential decrease(~160%at 10Hz)due to an increase in the double layer capacitance from secreted extracellular matrix(ECM)*** voltammetry(CV)sensor electrodes,fabricated on the bottom side of the membrane,enable sensing of molecular release at the site of cell culture without the need for downstream ***-time detection of ferrocene dimethanol injection across the membrane showed a three order-of-magnitude higher signal at the membrane than in the bulk media after reaching *** modular sensor-integrated transwell system allows unprecedented direct,real-time,and noninvasive access to physical and biochemical information,which cannot be obtained in a conventional transwell system.

关键词： transwell electrochemical culture

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Technology developments and biomedical applications of polarization-sensitive optical coherence tomography

引用

Frontiers of Optoelectronics 2015年第2期8卷 128-140页

作者： Zhenyang DING Chia-Pin LIANG Yu CHEN fischell department of bioengineering University of Maryland College Park MD 20742 USA

Polarization-sensitive optical coherence tomo- graphy （PS-OCT） enables depth-resolved mapping of sample polarization information, such as phase-retardation and optical axis orientation, which is particularly useful when the nano-scale organization of tissue that are difficult to be observed in the intensity images of a regular optical coherence tomography （OCT）. In this review, we survey two types of methods and systems of PS-OCT. The first type is PS-OCT with single input polarization state, which contain bulk optics or polarization maintaining fiber （PMF） based systems and single-mode fiber （SMF） based systems. The second type is PS-OCT with two different input polarization states, which contain SMF based systems and PMF based systems, through either time, frequency, or depth multiplexing. In addition, representa- tive biomedical applications using PS-OCT, such as retinal imaging, skin cancer detection, and brain mapping, are demonstrated.

关键词： optical coherence tomography (OCT) polar-ization-sensitive optical coherence tomography (PS-OCT),polarization imaging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A PERSONAL REFLECTION ON MY PH.D. ADVISOR DR. BRITTON CHANCE

引用

Journal of Innovative Optical Health Sciences 2011年第4期4卷 487-488页

作者： YU CHEN fischell department of bioengineering university of maryland 2218 Jeong H.Kim Building College ParkMD 20742USA

This article briefly reflects some of my interactions with *** Chance(BC),from a perspective of his graduate student.

关键词： Britton Chance reflection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

OPTIMIZATION OF DESIGN PARAMETERS FOR FLUORESCENCE LAMINAR OPTICAL TOMOGRAPHY

引用

Journal of Innovative Optical Health Sciences 2011年第3期4卷 309-323页

作者： CHAO-WEI CHEN YU CHEN fischell department of bioengineering and Electrical and Computer Engineering 2218 Jeong H.Kim Building University of Maryland College ParkMD 20742USA

Laminar optical tomography(LOT)is a mesoscopic tomographic imaging technique ranging between confocal microscopy and diffuse optical tomography(DOT).Fluorescence LOT(FLOT)provides depth-resolved molecular information with 100-200μm resolution over 2-3mm *** this study,we use Monte Carlo simulation and singular-value analysis(SVA)to optimize the source-detector configurations for potential enhancement of FLOT imaging *** effects of different design parameters,including source incidence and detector collection angles,detector number,and sampling density,are *** results indicate that angled incidence/detection configuration might improve the imaging resolution and depth sensitivity,especially for low-scattering *** the number of detectors and the number of scanning steps will also result in enhanced imaging *** also demonstrate that the optimal imaging performance depends upon the background scattering coe±*** result might provide an optimization strategy for FLOT or LOT experimental setup.

关键词： Laminar optical tomography(LOT) fluorescence laminar optical tomography(FLOT) singular value analysis(SVA) image reconstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Compound muscle action potential(CMAP)scan examination of paretic and contralateral muscles reveals motor unit alterations after stroke

引用

Science China(Life Sciences) 2023年第11期66卷 2604-2613页

作者： Maoqi Chen Zhiyuan Lu Xiaoyan Li Ya Zong Qing Xie Sheng Li Ping Zhou School of Rehabilitation Science and Engineering University of Health and Rehabilitation SciencesQingdao 266071China department of Neurology Medical College of WisconsinMilwaukee WI 53226USA fischell department of bioengineering University of Maryland at College ParkCollege Park MD 20742USA department of Rehabilitation Medicine Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China department of Physical Medicine and Rehabilitation University of Texas Health Science CenterHouston TX 77030USA TIRR Memorial Hermann Research Center TIRR Memorial Hermann HospitalHouston TX 77030USA

This study presents a novel compound muscle action potential(CMAP)examination of motor unit changes in paretic muscle post *** scan of the first dorsal interosseous(FDI)muscle was performed bilaterally in 16 chronic stroke *** parameters were derived from the CMAP scan to examine paretic muscle changes,including CMAP amplitude,D50,step index(STEPIX)and amplitude index(AMPIX).A significant decrease in CMAP amplitude and STEPIX was observed in paretic muscles compared with contralateral muscles(CMAP amplitude:paretic(9.0±0.5)mV,contralateral(11.3±0.9)mV,P=0.024;STEPIX:paretic 101.2±7.6,contralateral 121.9±6.5,P=0.020).No significant difference in D50 and AMPIX was observed between the paretic and contralateral sides(P>0.05).The findings revealed complex paretic muscle changes including motor unit degeneration,muscle fiber denervation,reinnervation and atrophy,providing useful insights to help understand neuromuscular mechanisms associated with weakness and other functional deterioration post *** CMAP scan experimental protocols and the applied processing methods are noninvasive,convenient,and automated,offering practical benefits for clinical application.

关键词： compound muscle action potential(CMAP)scan stroke motor unit muscle weakness step index(STEPIX) first dorsal interosseous(FDI)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：