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Treatment of Helicobactor pylori infection:analysis of Chinese clinical trials

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World Journal of Gastroenterology 2000年第3期6卷 324-325页

作者： Li YY Sha WH first Municipal peoples hosp Guangzhou Dept Gastroenterol Guangzhou 510180 Peoples R China

INTRODUCTION E radication of Helicobacter pylori(Hp)infection isgenerally not *** clinical regimens havebeen recommended in the *** theexperience from the other countries and the practicein China,Chinese doctors have tried *** this study,we collected and pooled thedata from Chinese literature to evaluate the effectof different regimens in Chinese patients infectedwith Hp.

关键词： Helicobacter infection/therapy clinical trials evaluating studies Helicobacter pylori

Distribution of gyrA mutations in fluoroquinolone-resistant Helicobacter pylori strains

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World Journal of Gastroenterology 2010年第18期16卷 2272-2277页

作者： Wang, Li-Hui Cheng, Hong Hu, Fu-Lian Li, Jiang Peking Univ first hosp Dept Gastroenterol Beijing 100034 Peoples R China

AIM:To investigate the resistance of Helicobacter pylori(***) to ciprofloxacin(CIP),levofloxacin(LVX) and moxifloxacin(MOX) in the Beijing area and to elucidate the resistance ***:Seventy-nine *** clinical strains,isolated from patients who had undergone upper gastrointestinal endoscopy in Peking University first hospital from 2007 to 2009,were tested for their susceptibility to CIP,LVX and MOX using the E-test *** strain 26695 was included in the susceptibility testing as a control *** to the minimal inhibitory concentration(MIC) values,a strain was classified as resistant to CIP,LVX or MOX when the MIC was > 1 μg/*** amplified by polymerase chain reaction(PCR) and sequenced the quinolone resistance-determining regions of the gyrA and gyrB genes from 29 quinolone-resistant and 16 quinolone-susceptible *** strains selected at ***:In this study,the resistance rates of *** to CIP,LVX or MOX were 55.7%(44/79),and the primary resistance rates were 26.6%(21/79).Patients with secondary resistance had received LVX in previous eradication treatments,but not MOX or ***-five strains,including 29 CIP,LVX or MOX-resistant strains(MIC:1.5-32 μg/mL) and 16 susceptible strains,were selected randomly from the 79 strains and used in PCR *** these 45 strains,27 resistant strains had mutations in the gyrA gene,including 11 strains with mutations corresponding to Asp-91(MIC:2-32 μg/mL),one of which also had a mutation corresponding to Val-150,and 16 strains had mutations at Asn-87(MIC:4-32 μg/mL),three of which also had mutations corresponding to Arg-140 or *** addition,Arg-140,Val-150 or Ala-97 mutations were separately detected in three susceptible *** of the gyrB gene showed that one strain of low resistance had a mutation corresponding to Ser-457 that coexisted with an Asp-91 *** was a significant difference in the occurrence of mutations in the gyrA gene between

关键词： Helicobacter pylori Antibiotic resistance Quinolones Mutation gyrA

Influential factors and effect evaluation of the intra-operative puncture biopsy for pancreatic masses

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Chinese Medical Journal 2012年第2期125卷 182-187页

作者： Zhuang Yan Yang Yin-mo Wang Wei-min Gao Hong-qiao Wan Yuan-lian Peking Univ first hosp Dept Gen Surg Beijing 100034 Peoples R China

Background It is a challenge for the surgeons to accurately diagnose the pancreatic masses preoperatively,which decides the choice of surgical managements and subsequently results in different survivor outcomes,operative complications,and mortality *** purposes of this study were to evaluate the diagnostic role that intra-operative puncture biopsy may play in pancreatic masses and to explore the relevant factors influencing the *** A retrospective study was performed on 94 in-patients admitted to Peking University first hospital for pancreatic masses during the period from June 1994 to December *** all underwent intra-operative puncture biopsy during exploratory *** sensitivity and specificity of intra-operative puncture biopsy were calculated and the relevant factors to the diagnosis of biopsy were selected for the statistical *** The overall sensitivity,specificity,positive predictive value,and negative predictive value of intra-operative puncture biopsy were 76.0%,94.7%,98.3% and 50.0%,*** analysis of bivariate correlations showed that the size of the pancreatic masses （P=-0.000）,the number of puncture biopsies （P=0.000）,and the presence of pancreatic fibrosis （P=-0.012） had statistic significance for the *** the multivariate analysis identified the size of the pancreatic masses （P=0.004） and the number of puncture biopsies （P=0.000） as independent predictive factors for intra-operative puncture *** addition,as the number of puncture biopsies increased,the sensitivity and specificity of diagnosis was improved （P=0.000）.The sensitivity and specificity of intra-operative puncture biopsy were found to be lower for the pancreatic masses less than 25 mm compared with the masses larger than 25 mm （P=0.000）.It was noted,however,that even if the masses were less than 25 mm,the sensitivity and specificity could be improved significantly as the number of puncture biopsies reached 3 to 6 （P=0.007

关键词： pancreas puncture biopsy intra-operative neoplasms

A new experimental model to study healing process of metaphyseal fracture

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Chinese Medical Journal 2012年第4期125卷 676-679页

作者： Han Na Zhang Pei-xun Wang Wei-bin Han Da-cheng Chen Jian-hai Zhan Hong-bo Jiang Bao-guo Peking Univ Peoples Hosp Dept Trauma & Orthopaed Beijing 100044 Peoples R China first hosp Qinhuangdao Qinhuangdao 066000 Hebei Peoples R China

Background There are few researches for the healing of metaphyseal fractures; moreover,the animal models to study the metaphyseal fractures are usually made by the oscillating saw osteotomy without reliable fixation,which is not in accordance with our current clinical *** this study,we established a new model to observe the healing process of metaphyseal *** Eighteen New Zealand rabbits were used in the *** fracture model was created by splitting the medial tibial plateau in rabbits,then reset,and fixed with compression *** 1,2,3,4,6,and 8 weeks postoperatively,the tibial specimens were collected; firstly,a general observation and an X-ray examination of the specimens was done,and then they were embedded in methylmethacrylate and cut into sections with hard tissue *** sections were stained with Giemsa reagent and examined under light *** There was no fracture displacement in the tibial specimens of all time points,except for one showing a *** external callus formation could be observed by X-ray and general *** 1 week of the operation,the fracture gap was filled by mesenchymal tissue; 2 weeks postoperatively,a large number of woven bones were formed; from the third week onwards,the woven bone began to turn into lamellar bone,and new trabecular structure began to *** all of the slices,no obvious chondrocytes formed in fracture areas; thus,there was no endochondral *** This model was an ideal fracture animal model and suitable for the study of metaphyseal fracture *** X-ray and histological images demonstrated that metaphyseal fracture healing was a process of direct bone healing through intramembranous bone formation under the conditions of minor trauma,good reduction,and firm fixation.

关键词： metaphysis fracture healing osteotomy histology experimental model

Is surgical treatment of cancer of the gastric cardia or esophagus associated with a concurrent major pulmonary operation feasible？One center＇s experience

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Chinese Medical Journal 2012年第2期125卷 193-196页

作者： Wang Xiao-xin Liu Tong-lin Wang Pei Li Jian Peking Univ first hosp Dept Thorac Surg Beijing 100034 Peoples R China

Background Pulmonary complications are a major cause of mortality after operation for cancer of the gastric cardia or *** the risk involved in gastric cardiectomy or esophagectomy associated with a concurrent major pulmonary operation is expected to be much higher,it has seldom been evaluated on the basis of clinical *** aim of this study was to investigate the possibility and feasibility of the gastric cardiectomy or esophagectomy associated with a major pulmonary *** From August 2003 to January 2011,14 patients underwent concurrent gastric cardiectomy or esophagectomy and a major pulmonary operation in our *** included eight for pulmonary invasion of esophageal carcinoma,and six for synchronous lung *** patients underwent systematic lymph node dissection for cardiac or esophageal *** prevent postoperative complications,the operative approach and dissection procedures for cardiac or esophageal carcinoma were modified according to the associated pulmonary operation and the extent of cancer *** thoracotomies for cardiectomy or esophagectomy were performed on the same side as the major pulmonary *** All patients underwent a curative *** were no deaths or postoperative complications in the six synchronous lung tumor *** the eight pulmonary invasion patients,one patient died of respiratory failure 11 days after operation,and postoperative complications developed in four of them,but none was *** patients were still *** Curative gastric cardiectomy or esophagectomy associated with concurrent major pulmonary operation is not contraindicated in patients in good *** selected patients,when the operative procedures for cardiectomy or esophagectomy are appropriately modified to minimize the effect of the associated pulmonary operation,the treatment is associated with a low operative morbidity and mortality with an acceptable long-term survival.

关键词： stomach neoplasms esophageal neoplasms lung neoplasms surgical treatment

Genome-wide Studies Reveal Genetic Risk Factors for Hepatic Fat Content

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GENOMICS PROTEOMICS & BIOINFORMATICS 2024年第2期22卷 qzae031页

作者： Li, Yanni van den Berg, Eline H. Kurilshikov, Alexander Zhernakova, Dasha, V Gacesa, Ranko Hu, Shixian Lopera-Maya, Esteban A. Zhernakova, Alexandra de Meijer, Vincent E. Sanna, Serena Dullaart, Robin P. F. Blokzijl, Hans Festen, Eleonora A. M. Fu, Jingyuan Weersma, Rinse K. Univ Groningen Univ Med Ctr Groningen Dept Gastroenterol & Hepatol NL-9713 GZ Groningen Netherlands Univ Groningen Univ Med Ctr Groningen Dept Genet NL-9713 GZ Groningen Netherlands Tianjin Med Univ Tianjin Med Univ Gen Hosp Dept Gastroenterol & Hepatol Tianjin 300052 Peoples R China Sun Yat Sen Univ Affiliated Hosp 1 Inst Precis Med Guangzhou 510080 Peoples R China ITMO Univ Ctr Comp Technol Lab Genom Divers St Petersburg 199034 Russia Univ Groningen Univ Med Ctr Groningen Dept Pediat NL-9713 GZ Groningen Netherlands Univ Groningen Univ Med Ctr Groningen Dept Surg Sect Hepatobiliary Surg & Liver Transplantat NL-9713 GZ Groningen Netherlands Univ Groningen Univ Med Ctr Groningen Dept Endocrinol NL-9713 GZ Groningen Netherlands

Genetic susceptibility to metabolic associated fatty liver disease (MAFLD) is complex and poorly characterized. Accurate characterization of the genetic background of hepatic fat content would provide insights into disease etiology and causality of risk factors. We performed genome-wide association study (GWAS) on two noninvasive definitions of hepatic fat content: magnetic resonance imaging proton density fat fraction (MRI-PDFF) in 16,050 participants and fatty liver index (FLI) in 388,701 participants from the United Kingdom (UK) Biobank (UKBB). Heritability, genetic overlap, and similarity between hepatic fat content phenotypes were analyzed, and replicated in 10,398 participants from the University Medical Center Groningen (UMCG) Genetics Lifelines Initiative (UGLI). Meta-analysis of GWASs of MRI-PDFF in UKBB revealed five statistically significant loci, including two novel genomic loci harboring CREB3L1 (rs72910057-T, P = 5.40E-09) and GCM1 (rs1491489378-T, P = 3.16E-09), respectively, as well as three previously reported loci: PNPLA3, TM6SF2, and APOE. GWAS of FLI in UKBB identified 196 genome-wide significant loci, of which 49 were replicated in UGLI, with top signals in ZPR1 (P = 3.35E-13) and FTO (P = 2.11E-09). Statistically significant genetic correlation (rg) between MRI-PDFF (UKBB) and FLI (UGLI) GWAS results was found (rg = 0.5276, P = 1.45E-03). Novel MRI-PDFF genetic signals (CREB3L1 and GCM1) were replicated in the FLI GWAS. We identified two novel genes for MRI-PDFF and 49 replicable loci for FLI. Despite a difference in hepatic fat content assessment between MRI-PDFF and FLI, a substantial similar genetic architecture was found. FLI is identified as an easy and reliable approach to study hepatic fat content at the population level.

关键词： Hepatic fat content MAFLD Genome-wide association study Fatty liver index Magnetic resonance imaging proton density fat fraction LIVER-DISEASE ASSOCIATION VARIANT LOCI SUSCEPTIBILITY PATHOGENESIS INSIGHTS TARGETS ENZYMES CONFERS

Role of BMP3 in progression of gastric carcinoma in Chinese people

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World Journal of Gastroenterology 2010年第11期16卷 1409-1413页

作者： Chen, Xue-Rong Wang, Jin-Wei Li, Xiang Zhang, Hong Ye, Zai-Yuan first peoples hosp Taizhou Dept Oncol Taizhou 318020 Zhejiang Peoples R China Zhejiang Prov hosp Dept Surg Oncol Hangzhou 310012 Zhejiang Peoples R China

AIM:To investigate the relation between gastric cancer and microsatellite instability(MSI),loss of heterozygosity(LOH)and promoter region ***:Fifty primary gastric carcinoma specimens were collected from patients with no family history of *** addition,normal tissues were also collected from patients as *** was extracted by polymerase chain reaction for single-strand conformation polymorphism,bisulfite DNA sequencing,and methylation-specific band ***:The positive rate for MSI and LOH in gastric carcinoma was 16%and 20%,*** to the tumor,node and metastasis staging system,the LOH frequency was higher in gastric carcinoma at stages Ⅲ and Ⅳ than in gastric carcinoma at stagesⅠ and Ⅱ(P=0.01),which was also significantly correlated with lymph node metastasis and clinicopathological characteristics of gastric *** of bone morphogenetic protein 3(BMP3)gene promoter was detected in 64.44% of gastric carcinoma tissue ***,no statistical significance was observed between promoter region methylation and carcinoma ***,the BMP3 gene methylation rate was 71.05% and 28.58%,respectively,in MSI positive and negative cases(P=0.031),suggesting that BMP3 genetic instability and promoter methylation are initiated during gastric *** was detected mostly in the late stages of gastric carcinoma,indicating that gastric carcinoma at late stages has a higher infiltration and a poorer ***:Promotor region methylation of the BMP3 gene may cause gastric carcinoma in Chinese people.

关键词： Bone morphogenetic protein 3 gene Gastric carcinoma Microsatellite instability Loss of heterozygocity Methylation

Pathological changes at early stage of multiple organ injury in a rat model of severe acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2010年第1期9卷 83-87页

作者： Zhang, Xi-Ping Zhang, Jie Ma, Mei-Li Cai, Yang Xu, Ru-Jun Xie, Qi Jiang, Xin-Ge Ye, Qian Hangzhou first peoples hosp Dept Gen Surg Hangzhou 310006 Zhejiang Peoples R China Hangzhou first peoples hosp Dept Pathol Hangzhou 310006 Zhejiang Peoples R China Zhejiang Univ Tradit Chinese Med Hangzhou 310053 Zhejiang Peoples R China

BACKGROUND: Severe acute pancreatitis (SAP) is a commonly seen acute abdominal syndrome characterized by sudden onset, rapid progression and high mortality rate. The damage in peripheral organs may be more severe than that in the pancreas, and can even lead to multiple organ dysfunction. It is critical to recognize early pathological changes in multiple organs. This study aimed to assess the early pathological features of damaged organs in a rat model of SAP. METHODS: Thirty clean grade healthy male Sprague-Dawley rats weighing 250-300 g were randomly divided into a model control group (n=15) and a sham-operated group (n=15). The SAP rat model was induced by sodium taurocholate. Samples of blood and from multiple organs were collected 3 hours after operation. We assessed the levels of IL-6, TNF-alpha, PLA2, NO, ET-1, MDA, amylases and endotoxin in blood and observed the early pathological changes in multiple damaged organs. RESULTS: Levels of IL-6, TNF-alpha, PLA2, NO, ET-1 and MDA in serum and of amylase and endotoxin in plasma of the model control group rats were significantly higher than those of the sham-operated group (P<0.01). Different degrees of pathological change were observed in multiple damaged organs. CONCLUSION: Multiple organ injury may occur at the early stage of SAP in rats.

关键词： severe acute pancreatitis pathological changes multiple organs injury

Non-traumatic diaphragmatic hernia of the liver in an adult:a case report

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Hepatobiliary & Pancreatic Diseases International 2007年第2期6卷 219-221页

作者： Luo, Hai-Feng Lei, Ting Wang, Hong-Jiang Tan, Guang Wang, Zhong-Yu Dalian Med Univ Dept Gen Surg First Affiliated Hosp Dalian 116011 Peoples R China Dalian Med Univ Dept Thorac Surg First Affiliated Hosp Dalian 116011 Peoples R China

BACKGROUND: Diaphragmatic hernia of the liver is a rare clinical entity, usually found after trauma in adults. This study was undertaken to elucidate a misdiagnosis of non-traumatic diaphragmatic hernia of the liver in an adult. METHOD: The clinical data of one patient with non-traumatic diaphragmatic hernia of the liver was analyzed. RESULTS: A tumor in the right lower thorax was revealed by chest X-ray and computed tomography. Non-traumatic diaphragmatic hernia of the liver was not identified until the operation. Pathological analysis confirmed the finding. The patient recovered well. CONCLUSIONS: Non-traumatic diaphragmatic hernia of the liver in an adult is a rare right-sided diaphragmatic hernia, which can move up into the chest cavity. It should be distinguished from lung cancer. The diagnosis and evaluation of non-traumatic diaphragmatic hernia of the liver can help optimize surgical management.

关键词： diaphragmatic hernia thorax liver management