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Tissue microarrays in pathological examination of apoptotic acinar cells induced by dexamethasone in the pancreas of rats with severe acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2007年第5期6卷 527-536页

作者： Zhang, Xi-Ping Tian, Hua Lu, Bei Chen, Li Xu, Ru-Jun Wang, Ke-Yi Wang, Zhi-Wei Cheng, Qi-Hui Shen, Hai-Ping Hangzhou first peoples hosp Dept Gen Surg Hangzhou 310006 Peoples R China Hangzhou first peoples hosp Dept Pathol Hangzhou 310006 Peoples R China Hangzhou first peoples hosp Cent Lab Hangzhou 310006 Peoples R China Hangzhou first peoples hosp Dept Obstet & Gynaecol Hangzhou 310006 Peoples R China Zhejiang Univ Sch Med Affiliated Hosp 2 Dept Gen Surg Hangzhou 310003 Peoples R China

BACKGROUND: The good therapeutic effects of large dose of dexamethasone on severe acute pancreatitis (SAP) patients have been proved. This study was designed to investigate the influence of dexamethasone on apoptosis of acinar cells in the pancreas of rats with SAP and the protein expression of the apoptosis-regulating genes Bax and Bcl-2. METHODS: Ninety Sprague-Dawley rats with SAP were randomly divided into a model group and a dexamethasone treated group (45 rats in each group), and another 45 rats formed the sham operation group. Survival rates were calculated and gross pathological changes in the pancreas of each group were observed under a light microscope 3, 6 and 12 hours after operation. Tissue microarray technology was applied to prepare pancreatic tissue sections. The changes in Bax and Bcl-2 protein expression levels of pancreatic tissues from each group were assessed by immunohistochemical staining, and TUNEL staining was used to evaluate changes in apoptosis index. RESULTS: The model and treated groups did not differ in mortality at each time point. The pathological score for the pancreas in the treated group was significantly lower than that in the model group at 3 and 6 hours. The positive rates of Bax protein expression in the head and tail of the pancreas in the treated group at all time points were all markedly higher than those of the model group. The positive rate of Bcl-2 protein expression in the head of the pancreas in the treated group was significantly higher than that of the model group at 3 hours. TUNEL staining showed that the pancreas head and tail apoptosis indices of the treated group were markedly higher than those of the model group after 6 hours. CONCLUSIONS: Apoptosis may be a protective response. to pancreatic cell injury. The mechanism of action of dexamethasone in treating SAP may be related to the apoptosis of acinar cells in the pancreas induced by apoptosis-regulating genes such as Bax and Bcl-2. The advantages of tissue mic

关键词： tissue microarrays severe acute pancreatitis dexamethasone apoptosis pancreas

Current concept of Spleen Stomach theory and Spleen deficiency syndrome in TCM

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World Journal of Gastroenterology 1998年第1期4卷 7-11页

作者： WU Xie Ning Shanghai first peoples hosp Dept Gastroenterol Shanghai 200080 Peoples R China Shanghai first peoples hosp Cent Res Lab Shanghai 200080 Peoples R China

ＣｕｒｅｎｔｃｏｎｃｅｐｔｏｆＳｐｌｅｅｎＳｔｏｍａｃｈｔｈｅｏｒｙａｎｄＳｐｌｅｅｎｄｅｆｉｃｉｅｎｃｙｓｙｎｄｒｏｍｅｉｎＴＣＭＷＵＸｉｅＮｉｎｇＳｕｂｊｅｃｔｈｅａｄｉｎｇｓｓｐｌｅｅｎｄｅｆｉｃｉｅｎｃｙ... 详细信息

ＣｕｒｅｎｔｃｏｎｃｅｐｔｏｆＳｐｌｅｅｎＳｔｏｍａｃｈｔｈｅｏｒｙａｎｄＳｐｌｅｅｎｄｅｆｉｃｉｅｎｃｙｓｙｎｄｒｏｍｅｉｎＴＣＭＷＵＸｉｅＮｉｎｇＳｕｂｊｅｃｔｈｅａｄｉｎｇｓｓｐｌｅｅｎｄｅｆｉｃｉｅｎｃｙｓｙｎｄｒｏｍｅ；ｓｐｌｅｅｎｓ...

关键词： spleen deficiency syndrome spleen stomach theory

Treatment of Helicobactor pylori infection:analysis of Chinese clinical trials

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World Journal of Gastroenterology 2000年第3期6卷 324-325页

作者： Li YY Sha WH first Municipal peoples hosp Guangzhou Dept Gastroenterol Guangzhou 510180 Peoples R China

INTRODUCTION E radication of Helicobacter pylori(Hp)infection isgenerally not *** clinical regimens havebeen recommended in the *** theexperience from the other countries and the practicein China,Chinese doctors have tried *** this study,we collected and pooled thedata from Chinese literature to evaluate the effectof different regimens in Chinese patients infectedwith Hp.

关键词： Helicobacter infection/therapy clinical trials evaluating studies Helicobacter pylori

Distribution of gyrA mutations in fluoroquinolone-resistant Helicobacter pylori strains

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World Journal of Gastroenterology 2010年第18期16卷 2272-2277页

作者： Wang, Li-Hui Cheng, Hong Hu, Fu-Lian Li, Jiang Peking Univ first hosp Dept Gastroenterol Beijing 100034 Peoples R China

AIM:To investigate the resistance of Helicobacter pylori(***) to ciprofloxacin(CIP),levofloxacin(LVX) and moxifloxacin(MOX) in the Beijing area and to elucidate the resistance ***:Seventy-nine *** clinical strains,isolated from patients who had undergone upper gastrointestinal endoscopy in Peking University first hospital from 2007 to 2009,were tested for their susceptibility to CIP,LVX and MOX using the E-test *** strain 26695 was included in the susceptibility testing as a control *** to the minimal inhibitory concentration(MIC) values,a strain was classified as resistant to CIP,LVX or MOX when the MIC was > 1 μg/*** amplified by polymerase chain reaction(PCR) and sequenced the quinolone resistance-determining regions of the gyrA and gyrB genes from 29 quinolone-resistant and 16 quinolone-susceptible *** strains selected at ***:In this study,the resistance rates of *** to CIP,LVX or MOX were 55.7%(44/79),and the primary resistance rates were 26.6%(21/79).Patients with secondary resistance had received LVX in previous eradication treatments,but not MOX or ***-five strains,including 29 CIP,LVX or MOX-resistant strains(MIC:1.5-32 μg/mL) and 16 susceptible strains,were selected randomly from the 79 strains and used in PCR *** these 45 strains,27 resistant strains had mutations in the gyrA gene,including 11 strains with mutations corresponding to Asp-91(MIC:2-32 μg/mL),one of which also had a mutation corresponding to Val-150,and 16 strains had mutations at Asn-87(MIC:4-32 μg/mL),three of which also had mutations corresponding to Arg-140 or *** addition,Arg-140,Val-150 or Ala-97 mutations were separately detected in three susceptible *** of the gyrB gene showed that one strain of low resistance had a mutation corresponding to Ser-457 that coexisted with an Asp-91 *** was a significant difference in the occurrence of mutations in the gyrA gene between

关键词： Helicobacter pylori Antibiotic resistance Quinolones Mutation gyrA

Updated overall survival and circulating tumor DNA analysis of ensartinib for crizotinib-refractory ALK-positive NSCLC from a phase II study

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Cancer Communications 2024年第4期44卷 455-468页

作者： Jing Zheng Tao Wang Yunpeng Yang Jie Huang Jifeng Feng Wu Zhuang Jianhua Chen Jun Zhao Wei Zhong Yanqiu Zhao Yiping Zhang Yong Song Yi Hu Zhuang Yu Youling Gong Yuan Chen Feng Ye Shucai Zhang Lejie Cao Yun Fan Gang Wu Yubiao Guo Chengzhi Zhou Kewei Ma Jian Fang Weineng Feng Yunpeng Liu Zhendong Zheng Gaofeng Li Huijie Wang Shundong Cang Ning Wu Wei Song Xiaoqing Liu Shijun Zhao Lieming Ding Giovanni Selvaggi Yang Wang Shanshan Xiao Qian Wang Zhilin Shen Jianya Zhou Jianying Zhou Li Zhang Department of Respiratory Disease Thoracic Disease CenterThe First Affiliated HospitalCollege of MedicineZhejiang UniversityZhejiang Provincial Clinical Research Center for Respiratory DiseaseHangzhouZhejiangP.R.China Hangzhou Repugene Technology Co. LtdHangzhouZhejiangP.R.China Department of Medical Oncology Sun Yat-sen University Cancer Cen-terState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Medical Oncology Jiangsu Cancer HospitalJiangsu Institute of Cancer ResearchThe Affiliated Cancer Hospital of Nanjing Medical UniversityNanjingJiangsuP.R.China Department of Thoracic Oncology Fujian Provincial Cancer HospitalFujian Medical University Cancer HospitalFuzhouFujianP.R.China Department of Medical Oncology-Chest Hunan Cancer HospitalChangshaHunanP.R.China Department of Thoracic Oncology Beijing Cancer HospitalBeijingP.R.China Department of Pulmonary Medicine Peking Union Medical College HospitalChinese Academy of Medical SciencesPeking Union Medical CollegeBeijingP.R.China Respiratory Department of Internal Medicine Henan Provincial Can-cer HospitalAffiliated Cancer Hospital of Zhengzhou UniversityZhengzhouHenanP.R.China Thoracic Medical Oncology Zhejiang Cancer HospitalHangzhouZhejiangP.R.China Division of Respiratory Medicine Jinling HospitalNanjing University School of MedicineNanjingJiangsuP.R.China Department of Oncology Chinese People’s Liberation Army(PLA)General HospitalBeijingP.R.China Department of Oncology The Affiliated Hospital of Qingdao UniversityQingdaoShandongP.R.China Department of Thoracic Oncology Cancer CenterWest China HospitalSichuan UniversityChengduSichuanP.R.China Department of Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China Department of Medical Oncology Cancer HospitalThe First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityTeaching Hospital of

Background:The initial phase II stuty(NCT03215693)demonstrated that ensartinib has shown clinical activity in patients with advanced crizotinib-refractory,anaplastic lymphoma kinase(ALK)-positive non-small cell lung cancer(NSCLC).Herein,we reported the updated data on overall survival(OS)and molecular profiling from the initial phase Ⅱ ***:In this study,180 patients received 225 mg of ensartinib orally once daily until disease progression,death or *** was estimated by Kaplan‒Meier methods with two-sided 95%confidence intervals(CIs).Next-generation sequencing was employed to explore prognostic biomarkers based on plasma samples collected at baseline and after initiating *** tumor DNA(ctDNA)was detected to dynamically monitor the genomic alterna-tions during treatment and indicate the existence of molecular residual disease,facilitating improvement of clinical ***:At the data cut-off date(August 31,2022),with a median follow-up time of 53.2 months,97 of 180(53.9%)patients had *** median OS was 42.8 months(95%CI:29.3-53.2 months).A total of 333 plasma samples from 168 patients were included for ctDNA *** inferior OS correlated sig-nificantly with baseline ALK or tumor protein 53(TP53)*** addition,patients with concurrent TP53 mutations had shorter OS than those without con-current TP53 *** ctDNA levels evaluated by variant allele frequency(VAF)and haploid genome equivalents per milliliter of plasma(hGE/mL)at baseline were associated with poor ***,patients with ctDNA clear-ance at 6 weeks and slow ascent growth had dramatically longer OS than those with ctDNA residual and fast ascent growth,***,patients who had a lower tumor burden,as evaluated by the diameter of target lesions,had a longer *** Cox regression analysis further uncovered the independent prognostic values of bone metastases,higher hGE,and elevated ALK mutation abundance at 6 we

关键词： anaplastic lymphoma kinase ctDNA ensartinib non-small cell lung cancer overall survival

Clinical value of contrast-enhanced ultrasonography in the characterization of focal liver lesions:a prospective multicenter trial

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Hepatobiliary & Pancreatic Diseases International 2009年第4期8卷 370-376页

作者： Wang, Wen-Ping Wu, Ying Luo, Yan Li, Rui Zhou, Xiao-Dong Zhang, Jun Qian, Chao-Wen Tan, Xu-Yan Xu, Qing-Hua Wang, Yan Yuan, Jian-Jun Fudan Univ Zhongshan Hosp Dept Diagnost Ultrasound Shanghai 200032 Peoples R China Shenzhen peoples hosp Dept Ultrasound Shenzhen 518020 Peoples R China Sichuan Univ Dept Ultrasound W China Hosp Chengdu 610041 Peoples R China Chongqing SW hosp Dept Ultrasound Chongqing 400038 Peoples R China Fourth Mil Med Univ Dept Diagnost Ultrasound Xijing Hosp Xian 710032 Peoples R China Jilin Prov Canc hosp Dept Electrodiag Changchun 130012 Peoples R China Zhejiang Prov Canc hosp Dept Ultrasound Hangzhou 310022 Zhejiang Peoples R China Jiangsu Prov Canc hosp Dept Ultrasound Nanjing 210009 Peoples R China first peoples hosp foshan Dept Diagnost Ultrasound Foshan 528000 Peoples R China Henan Prov Canc hosp Dept Ultrasound Zhengzhou 450003 Peoples R China peoples hosp Henan Prov Dept Diagnost Ultrasound Zhengzhou 450003 Peoples R China

BACKGROUND: Contrast-enhanced ultrasonography (CEUS) is increasingly accepted in clinical settings for diagnostic imaging of focal liver lesions (FLLs). This study aimed to assess the efficacy of CEUS in the characterization of FLLs in comparison with final diagnosis based on gold standard assessment. METHODS: The study was approved by the local ethics committee and participating patients provided written informed consent. A total of 148 patients with 164 FLLs were studied. Unenhanced ultrasonography (US) and CEUS were performed using fundamental and harmonic imaging, respectively. Contrast enhancement was assessed during the arterial, portal and late vascular phases after intravenous administration of contrast (SonoVue (R), Bracco, Italy). Sensitivity, specificity and diagnostic accuracy of US and CEUS were compared in identifying the lesion as benign, malignant or indeterminate and its actual tumor type. Final diagnosis was established by biopsy (129/164), MR imaging (11/164) or medical history (24/164). RESULTS: When compared to the gold standard, the number of indeterminate diagnoses was reduced from 56.7% (93/164) as assessed by fundamental imaging to 6.1% (10/164) after SonoVue (R) enhanced US examination. Sensitivity and specificity improved from 49% and 25% at baseline US to 93% and 75% with CEUS, respectively (P<0.01). Diagnostic accuracy of CEUS was 88% in contrast to 41% of baseline US. CONCLUSION: SonoVue (R) enhanced US improves the characterization of FLLs and may limit the need for further investigations.

关键词： contrast-enhanced ultrasonography characterization focal liver lesions

Role of BMP3 in progression of gastric carcinoma in Chinese people

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World Journal of Gastroenterology 2010年第11期16卷 1409-1413页

作者： Chen, Xue-Rong Wang, Jin-Wei Li, Xiang Zhang, Hong Ye, Zai-Yuan first peoples hosp Taizhou Dept Oncol Taizhou 318020 Zhejiang Peoples R China Zhejiang Prov hosp Dept Surg Oncol Hangzhou 310012 Zhejiang Peoples R China

AIM:To investigate the relation between gastric cancer and microsatellite instability(MSI),loss of heterozygosity(LOH)and promoter region ***:Fifty primary gastric carcinoma specimens were collected from patients with no family history of *** addition,normal tissues were also collected from patients as *** was extracted by polymerase chain reaction for single-strand conformation polymorphism,bisulfite DNA sequencing,and methylation-specific band ***:The positive rate for MSI and LOH in gastric carcinoma was 16%and 20%,*** to the tumor,node and metastasis staging system,the LOH frequency was higher in gastric carcinoma at stages Ⅲ and Ⅳ than in gastric carcinoma at stagesⅠ and Ⅱ(P=0.01),which was also significantly correlated with lymph node metastasis and clinicopathological characteristics of gastric *** of bone morphogenetic protein 3(BMP3)gene promoter was detected in 64.44% of gastric carcinoma tissue ***,no statistical significance was observed between promoter region methylation and carcinoma ***,the BMP3 gene methylation rate was 71.05% and 28.58%,respectively,in MSI positive and negative cases(P=0.031),suggesting that BMP3 genetic instability and promoter methylation are initiated during gastric *** was detected mostly in the late stages of gastric carcinoma,indicating that gastric carcinoma at late stages has a higher infiltration and a poorer ***:Promotor region methylation of the BMP3 gene may cause gastric carcinoma in Chinese people.

关键词： Bone morphogenetic protein 3 gene Gastric carcinoma Microsatellite instability Loss of heterozygocity Methylation

Pathological changes at early stage of multiple organ injury in a rat model of severe acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2010年第1期9卷 83-87页

作者： Zhang, Xi-Ping Zhang, Jie Ma, Mei-Li Cai, Yang Xu, Ru-Jun Xie, Qi Jiang, Xin-Ge Ye, Qian Hangzhou first peoples hosp Dept Gen Surg Hangzhou 310006 Zhejiang Peoples R China Hangzhou first peoples hosp Dept Pathol Hangzhou 310006 Zhejiang Peoples R China Zhejiang Univ Tradit Chinese Med Hangzhou 310053 Zhejiang Peoples R China

BACKGROUND: Severe acute pancreatitis (SAP) is a commonly seen acute abdominal syndrome characterized by sudden onset, rapid progression and high mortality rate. The damage in peripheral organs may be more severe than that in the pancreas, and can even lead to multiple organ dysfunction. It is critical to recognize early pathological changes in multiple organs. This study aimed to assess the early pathological features of damaged organs in a rat model of SAP. METHODS: Thirty clean grade healthy male Sprague-Dawley rats weighing 250-300 g were randomly divided into a model control group (n=15) and a sham-operated group (n=15). The SAP rat model was induced by sodium taurocholate. Samples of blood and from multiple organs were collected 3 hours after operation. We assessed the levels of IL-6, TNF-alpha, PLA2, NO, ET-1, MDA, amylases and endotoxin in blood and observed the early pathological changes in multiple damaged organs. RESULTS: Levels of IL-6, TNF-alpha, PLA2, NO, ET-1 and MDA in serum and of amylase and endotoxin in plasma of the model control group rats were significantly higher than those of the sham-operated group (P<0.01). Different degrees of pathological change were observed in multiple damaged organs. CONCLUSION: Multiple organ injury may occur at the early stage of SAP in rats.

关键词： severe acute pancreatitis pathological changes multiple organs injury

Non-traumatic diaphragmatic hernia of the liver in an adult:a case report

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Hepatobiliary & Pancreatic Diseases International 2007年第2期6卷 219-221页

作者： Luo, Hai-Feng Lei, Ting Wang, Hong-Jiang Tan, Guang Wang, Zhong-Yu Dalian Med Univ Dept Gen Surg First Affiliated Hosp Dalian 116011 Peoples R China Dalian Med Univ Dept Thorac Surg First Affiliated Hosp Dalian 116011 Peoples R China

BACKGROUND: Diaphragmatic hernia of the liver is a rare clinical entity, usually found after trauma in adults. This study was undertaken to elucidate a misdiagnosis of non-traumatic diaphragmatic hernia of the liver in an adult. METHOD: The clinical data of one patient with non-traumatic diaphragmatic hernia of the liver was analyzed. RESULTS: A tumor in the right lower thorax was revealed by chest X-ray and computed tomography. Non-traumatic diaphragmatic hernia of the liver was not identified until the operation. Pathological analysis confirmed the finding. The patient recovered well. CONCLUSIONS: Non-traumatic diaphragmatic hernia of the liver in an adult is a rare right-sided diaphragmatic hernia, which can move up into the chest cavity. It should be distinguished from lung cancer. The diagnosis and evaluation of non-traumatic diaphragmatic hernia of the liver can help optimize surgical management.

关键词： diaphragmatic hernia thorax liver management