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DNA barcoding in closely related species:A case study of Primula *** Pax(Primulaceae) in China

Journal of Systematics and Evolution 2011年第3期49卷 225-236页

作者： 1Hai-Fei YAN 2Gang HAO 1Chi-Ming HU 1Xue-Jun GE* 1(Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China) 2(College of Life Sciences,South China Agricultural University,Guangzhou 510642,China) (Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China) (College of Life Sciences South China Agricultural University Guangzhou 510642 China) *Correspondence: Xue-Jun GE *Correspondence: ?Author for correspondence. E-mail: xjge@*** Tel./fax: 86-20-37252551.

DNA barcoding is a method of identifying species by analyzing one or a few short standardized DNA *** are particular challenges in barcoding plants,especially for distinguishing closely related ***,there is an urgent need to evaluate the performance of candidate loci for distinguishing between species,especially closely related species,to complement the rbcL + matK combination suggested as the core barcode for land *** sampled 48 individuals representing 12 species in Primula *** Pax in China to evaluate the performance of eight leading candidate barcode loci(matK,rbcL,rpoB,rpoC1,trnH-psbA,psbK-psbI,atpFatpH,and internal transcribed spacer(ITS)).The core combination rbcL + matK gave only 50% species resolution in *** terms of intraspecies and interspecies divergence,degree of monophyly,and sequence similarity,ITS,trnH-psbA,and psbK-psbI showed good performance as single-locus *** transcribed spacer displayed the highest genetic divergence and best discriminatory power,both alone and in combination with rbcL + matK(83.3% species resolution).We recommend evaluating the use of ITS for barcoding in other *** or single copy nuclear regions would provide more sophisticated barcoding tools in the long term,even though further research is required to find suitable loci.

关键词： DNA barcoding ITS plastid candidates Primlula sect.Proliferae species discrimination.

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A new orchid Paphiopedilum guangdongense and its molecular evidence

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Journal of Systematics and Evolution 2010年第5期48卷 350-355页

作者： 1 Li-Jun CHEN 1,2,3Zhong-Jian LIU 4You-Yu LI 1Li-Qiang LI 1(Shenzhen Key Laboratory for Orchid Conservation and Utilization,The National Orchid Conservation Center of China/The Orchid Conservation and Research Center of Shenzhen,Shenzhen 518114,China) 2(The Center for Biotechnology and BioMedicine,Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China) 3(College of Forestry,South China Agricultural University,Guangzhou 510642,China) 4(The Nature Reserve of Dawuling of Guangdong Province,Xinyi 525000,Guangdong,China) (Shenzhen Key Laboratory for Orchid Conservation and Utilization The National Orchid Conservation Center of China/The Orchid Conservation and Research Center of Shenzhen Shenzhen 518114 China) (The Center for Biotechnology and BioMedicine Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China) (College of Forestry South China Agricultural University Guangzhou 510642 China) (The Nature Reserve of Dawuling of Guangdong Province Xinyi 525000 Guangdong China) *Correspondence: Zhong-Jian LIU *Correspondence: ?Author for correspondence. E-mail: liuzj@*** Tel.: 86-755-25712359 fax: 86-755-25711928.

A new Paphiopedilum entity found in southern Guangdong was studied taxonomically by means of internal transcribed spacer of nuclear ribosomal DNA analysis in addition to morphological and phytogeographical *** entity is similar to Paphiopedilum gratrixianum(Masters)Rolfe morphologically,from which it differs by having narrower leaves not wider than 2 cm and lacking purple spots toward abaxial base,pale green dorsal sepal veined with pale purplish brown and not heavily spotted with purple,and staminode subtruncate and mucronate at *** analysis of internal transcribed spacer sequences of this entity and its allies came to the same conclusion that the entity is a member of Paphiopedilum section Paphiopedilum and closely related to but distinct from *** a result,it is treated here as a new species,Paphiopedilum guangdongense ***&***.

关键词： new species nrITS Orchidaceae Paphiopedilum guangdongense.

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Molecular phylogeny of Osmanthus(Oleaceae) based on non-coding chloroplast and nuclear ribosomal internal transcribed spacer regions

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Journal of Systematics and Evolution 2010年第6期48卷 482-489页

作者： 1,2Wang-Jun YUAN 1Wei-Rui ZHANG 1Yuan-Ji HAN 1Mei-Fang DONG 1Fu-De SHANG 1(College of Life Sciences,Henan University,Kaifeng 475004,China) 2(Institute of Chinese Materia Medica,Henan University,Kaifeng 475004,China) (College of Life Sciences Henan University Kaifeng 475004 China) (Institute of Chinese Materia Medica Henan University Kaifeng 475004 China) *Correspondence: Wang-Jun YUAN Fu-De SHANG *Correspondence: Author for correspondence. F-D SHANG E-mail: fudeshang@*** Tel. & fax: 86-378-3886199.All authors contributed equally to this work.

The phylogenetic relationships of Osmanthus *** investigated using the nuclear ribosomal internal transcribed spacer(ITS) regions and non-coding chloroplast regions(psbA-trnH,trnL-F).The two datasets support the conclusion that Osmanthus is polyphyletic,with some species of the subtribe Oleinae nested within *** didymopetalus *** is nested within the clade formed by species of section Osmanthus in two *** attenuatus ***,*** ***,and *** *** of traditional section Osmanthus are clearly divergent from other accessions,and do not form a monophyletic group with other Osmanthus *** marginatus *** embedded in the clade formed by species of section Osmanthus in the ITS *** cpDNA trees all species of section Osmanthus are placed in the large clade and all species of section Leiolea formed a *** taxonomic incongruence among trees for ITS and cpDNA indicate hybridization,as introgression may have occurred among some species of sections Osmanthus and *** of Osmanthus is discussed in light of molecular and morphological data,and a revised infrageneric classification with three sections(Leiolea,Siphosmanthu,and Osmanthus) is *** section Linocieroides is abandoned and united with section Osmanthus.

关键词： ITS molecular phylogeny Osmanthus psbA-trnH trnL-F.

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Features and distribution patterns of Chinese endemic seed plant species

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Journal of Systematics and Evolution 2011年第2期49卷 81-94页

We compiled and identified a list of Chinese endemic seed plant species based on a large number of published references and expert reviews. The characters of these seed plant species and their distribution patterns were described at length. China is rich in endemic seed plants, with a total of 14 939 species (accounting for 52.1% of its total seed plant species) belonging to 1584 genera and 191 families. Temperate families and genera have a significantly higher proportion of endemism than cosmopolitan and tropical ones. The most primitive and derived groups have significantly higher endemism than the other groups. The endemism of tree, shrub, and liana or vine is higher than that of total species; in contrast, the endemism of herb is lower than that of total species. Geographically, these Chinese endemic plants are mainly distributed in Yunnan and Sichuan provinces, southwest China. Species richness and proportion of these endemic plants decrease with increased latitude and have a unimodal response to altitude. The peak value of proportion of endemism is at higher altitudes than that of total species and endemic species richness. The proportions of endemic shrub, liana or vine, and herb increase with altitude and have a clear unimodal curve. In contrast, the proportion of tree increases with altitude, with a sudden increase at ～4000 m and has a completely different model. To date, our study provides the most comprehensive list of Chinese endemic seed plant species and their basic composition and distribution features.

关键词： biodiversity conservation distribution pattern endemism flora of China seed plants.

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Integrative Stability Analysis for A Class of Intelligent Control Systems

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Journal of Systems Engineering and Electronics 1996年第4期7卷 13-18页

作者： Qin Shiyin(Institute of Systems Engineering, Xi’an Jiaotong University,Xi’an 710049, P.R. China, Tel. (029)3235011-3677, fax (029)3237910) Institute of Systems Engineering Xi'an Jiaotong University Xi'an 710049 P.R. China Tel. (029)3235011-3677 Fax (029)3237910

In this paper the integrative stability is studied for a class of intelligent control systems which are described by an octette structural model. Based on the definitions Of state reachability and stabilizability of i... 详细信息

关键词： Integrative stability Intelligent control systems Systems Engineering.

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The αMβ2 integrin and its role in neutrophil function

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Cell Research 1999年第3期9卷 171-178页

作者： ZHANG LI (Department of Vascular Biology, American Red Cross,Holland La6oratory, 15601 Crabbs Branch Way, Rockville,MD 20855 USA)Tel: 301-738-0657 fax: 301-738-0465e-mail: zhangl@u8a.redcross. org 1Department of Vascular Biology American Red Cross Holland Laboratory 15601 Crabbs Branch Way Rockville MD 20855 USA Tel: 301-738-0657 Fax: 301-738-0465 e-mail: zhangl@***

Neutrophils are the first cell type to arrive at the in-jury sites and play a critical role in host defense, by virtueof its ability to adhere and transmigrate through endothe-lium, to phagocytose foreign pathogens, and to producefree oxygen radicals and proteolytic enzymes. Yet, inap-propriate neutrophil activation causes tissue damage andvarious inflammatory diseases. These physiological andpathological functions of neutrophils depend on the en-gagement of certain surface receptors, especially αMβ2,the ma jor β2 integrin receptor present on neutrophil sur-face. Understanding of the molecular mechanisms under-lying ligand binding by αMβ2, as well as the roles of αMβ2-ligand interactions in neutrophil functions will enable usto regulate more precisely neutrophil activities: that is,to promote their host defense functions, and at the sametime to minimize their deleterious effects on normal cells.

关键词： Leukocyte integrn receptor adhesion

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Experimental evaluation of design methods for in-site detention ponds

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International Journal of Sediment Research 2010年第1期25卷 52-63页

作者： Yao-Ming HONG Department of Design for Sustainable Environment,MingDao University,369 Wen-Hwa Rd.,Peetow,Changhua 52345,Taiwan,China [a] Assis. Prof. Department of Design for Sustainable Environment MingDao University 369 Wen-Hwa Rd. Peetow Changhua 52345 Taiwan China Telephone: 886.4.8876660 ext 8613 Fax: 886.4.8886927

In this study,the detention rate of detention pond was defined to include both reduction and delay of peak *** routing experiments,with various combinations of inflow hydrographs and outlet devices in an in-site detention pond,were performed to examine the effectiveness of two analysis methods,namely the simplified method and the hydrological routing method;and to compare the detention rate of two types of outlet,namely the rectangular sharp-crested weir and the rectangular *** between predicted results and experimental outcomes show that the estimation errors of maximum storage volume obtained by the hydrological routing method are less than 5%,while those achieved by the simplified method are less than 10%.Hence,the simplified method with easier calculation but poorer accuracy may be employed to estimate the maximum storage volume in the preliminary design stage,while the hydrological routing method,which involves more complicated calculation but gives more precise estimation,is better suited for application in the detailed design ***,the maximum storage volume of the rectangular orifice is smaller than that of rectangular sharp-crested weir under the same peak flow reduction,making the rectangular orifice a better choice of outlet *** addition,inflow hydrograph with a long recession time or a long rainfall duration needs a long delay time to reach the required reduction in peak discharge.

关键词： Detention pond Hydrological routing method Simplified method Detention rate Experiments

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A MODIFIED FULL-ARRAY ARCHITECTURE FOR 64 x 64 BIT MULTIPLIER

A MODIFIED FULL-ARRAY ARCHITECTURE FOR 64 x 64 BIT MULTIPLIE...

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The Third International Conference on Solid State and Integrated Circuit Technology

作者： Minkyu Song Hocheol Park Changwoo Jee Jongsoo Lee G4fax Lab. KAITECH SEOUL KOREA G4fax Lab. KAITECH SEOUL KOREA G4fax Lab. KAITECH SEOUL KOREA G4fax Lab. KAITECH SEOUL KOREA

＜正＞ In this paper,a 64 x 64 bit modified full-array multiplier using a new compression technique is *** multiplier is based on a modular array architecture with the block of an encoder to implement the modified Booth’s algorithm [1],the block implementing the Wallace’s tree [2],and the block of a 128-bit Carry Look-Ahead（CLA） *** input of an encoder is 16-bit long and the block implementing the Wallace’s tree consists of both 4-2 compressors and 6-2 compressors proposed in this *** those compressors,the propagation delay time of the proposed multiplier is about 5% reduced,compared to the multiplier which has a parallel structured architecture [4].Also the multiplier has easier design methodology due to the regular characteristics of the modular array architecture.

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趋化素样因子(CKLF1)对骨髓细胞增殖活性的研究

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中国医学科学院学报 2001年第2期23卷 119-122页

作者：韩文玲芮珉张颖妹陈英玉钟英成狄春辉宋泉声马大龙北京大学基础医学院免疫系 fax: 010-62091149

目的研究趋化素样因子 1(CKLF1)对造血功能的调节作用。方法利用 MTT法，检测 CKLF1真核细胞表达载体转染上清在液体培养基中，对人低密度骨髓细胞和小鼠骨髓细胞的增殖调控作用；并在半固体培养基中，观察其对人低密度骨髓细胞集落... 详细信息

目的研究趋化素样因子 1(CKLF1)对造血功能的调节作用。方法利用 MTT法，检测 CKLF1真核细胞表达载体转染上清在液体培养基中，对人低密度骨髓细胞和小鼠骨髓细胞的增殖调控作用；并在半固体培养基中，观察其对人低密度骨髓细胞集落形成的刺激作用。结果 COS-7细胞表达的 CKLF1能明显促进人和小鼠骨髓细胞的增殖，并能促进人骨髓细胞的集落形成，与 GM-CSF有协同刺激作用。结论 CKLF1能促进人和小鼠骨髓造血干

关键词：趋化素样因子1 造血干细胞集落形成骨髓细胞增殖

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A new synonym of Pedicularis cyathophylla(Orobanchaceae)

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Journal of Systematics and Evolution 2011年第5期49卷 507-507页

作者： 1,2~Wen-Bin YU 1,2~Pan-Hui HUANG 1~Hong WANG 1(Key Laboratory of Biodiversity and Biogeography,and Plant Germplasm and Genomics Center,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China) 2(Graduate University of Chinese Academy of Sciences,Beijing 100049,China) (Key Laboratory of Biodiversity and Biogeography and Plant Germplasm and Genomics Center Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Graduate University of Chinese Academy of Sciences Beijing 100049 China) *Correspondence: Hong WANG *Correspondence: E-mail: wanghong@*** Tel.: 86-871-5223534 fax: 86-871-5217791.

Pedicularis cyathophylla Franchet in Bull. Soc. Bot. France 47: ***: China. Sichuan: Kangding, Zheduoshan Mountains(Ta-tsien-kou & Tche-to-chan), J. A. Soulie′ 218 (holotype,P00160827, P!).Pedicularis xiangchengensis... 详细信息

关键词： PE A new synonym of Pedicularis cyathophylla Orobanchaceae

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