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Diversity and Dynamics of Soil Free-Living Nematode Populations in a Mediterranean Agroecosystem

Pedosphere 2005年第2期15卷 204-215页

作者： LIANGWen-Ju I.LAVIAN S.PEN-MOURATOV Y.STEINBERGER facultyoflifesciences Bar-IlanUniversityRamat-Gan52900（Israel）.／／InstituteofAppliedEcologyChineseAcademyofSciencesShenyang110016（China） facultyoflifesciences Bar-IlanUniversityRamat-Gan52900（Israel）

To determine the effect of agricultural management on the dynamics and functional diversity of soil nematode communities in a carrot field at Kibbutz Ramat Hakovesh, Israel, soil samples from 0-10 cm and 10-20 cm depths were collected during the growing season of carrot. Indices were used to compare and assess the response of soil free-living nematode communities to agricultural management. Eighteen nematode families and 20 genera were observed during the growing period, with Cephalobus, Rhabditidae, Aphelenchus, Tylenchus, and Dorylaimus being the dominant genera/*** the planting, mid-season and post-harvest periods the total number of nematodes at both depths was significantly lower (P ＜ 0.01) in the carrot treatment than in the control plots, while during the harvest period at both depths total nematodes and bacterivores were significantly higher in the treatment plots (P ＜ 0.01). The values of the maturity index (MI) at both depths were found to be significantly lower in the treatment plots than in the control plots during the pre-planting period (P ＜ 0.05). Overall, WI, MI and PPI were found to be more sensitive indicators than other ecological indices for assessing the response of nematode communities to agricultural management in a Mediterranean agroecosystem.

关键词：农业管理胡萝卜生长季节成熟指数线虫群落

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Ginkgolide K protects cardiomyocytes against ER stress through stimulating ER-associated degradation （ERAD） and autophagy

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中国药理学通报 2015年第B11期31卷 181-182页

作者： WANGShou-bao WANGZhen-zhong FANQi-ru DUGuan-hua WANGXin XIAOWei facultyoflifesciences TheUniversityofManchesterManchesterM139NTUK

Aim Endoplasmic reticulum （ER） stress is increasingly recognized as an important contributor to the pathophysiology of many diseases, and therapeutic interventions that target ER stress response emerge as new thera- peutic modalities to treat cardiovascular diseases driven by prolonged ER stress. Ginkgolides K （GK） is a diterpene lactone constituent isolated from the leaves of Ginkgo biloba and has been found to possess potent neuroprotective properties. This study is aimed to investigate the cytoprotective effect of GK in cultured cardiomyocytes subjected to ER stress injury. Neonatal rat cardiomyocytes （NRCMs） were treated with ER stress inducer tunicamycin to mimic the ER stress injury. We demonstrated that GK pre-treatment mitigated ER stress-induced apoptosis in tunicamycin treated NRCMs. We observed that the activation of ER-associated degradation （ERAD） and autophagy were in- volved in the ER stress inhibition exerted by GK. These beneficial effects of GK were nearly abolished by the addi- tion of specific short interfering RNA （siRNA） for IRElα and XBP-1. Therefore, we conclude that GK might be a promising therapeutic agent for ER stress-mediated cardiovascular diseases, and ER-associated degradation （ERAD） and autophagy play a vital role in GK mediated cytoprotection.

关键词： Ginkgolide K ER stress Neonatal rat cardiomyocytes (NRCMs) ER-associated degradation (ERAD) Autophagy

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A High-affinity Activator of G551D-CFTR Chloride Channel Identified By High Throughput Screening

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Chemical Research in Chinese Universities 2004年第6期20卷 738-742页

作者： ZHAOLu HECheng-yan LIUYan-li ZHOUHong-lan ZHOUJin-song SHANGDe-jing YANGHong MembraneChannelResearchLaboratory NortheastNormalUniversityChangchun130024P.R.China China-JapanUnionHospital JilinUniversityChangchun130033P.R.China facultyoflifesciences LiaoningNormalUniversityDalian116029P.R.China

A stably transfected CHO cell line coexpressing G551D-CFTR and iodide-sensitive yellow fluorescent protein mutant EYFP-H148Q-I152L was successfully established and used as assay model to identify small-molecule activators of G551D-CFTR chloride channel from 100000 diverse combinatorial compounds by high throughput screening on a customized Beckman robotic system. A bicyclooctane compound was identified to activate G551D-CFTR chloride channel with high-affinity(K d=1.8 μmol/L). The activity of the bicyclooctane compound is G551D-CFTR-specific, reversible and non-toxic. The G551D-CFTR activator may be useful as a tool to study the mutant G551D-CFTR chloride channel structure and transport properties and as a candidate drug to cure cystic fibrosis caused by G551D-CFTR mutation.

关键词： Cystic fibrosis Yellow fluorescent protein(YFP) High throughput screening(HTS) Small molecule

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A Class of High-affinity Bicyclooctane G551D-CFTR Activators Identified by High Throughput Screening

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Chemical Research in Chinese Universities 2004年第6期20卷 743-746页

作者： HECheng-yan ZHAOLu LIUYan-li XULi-na SHANGDe-jing YANGHong China-JapanUnionHospital JilinUniversityChangchun130033P.R.China CollegeofTraditionalChineseMedicineMaterial JilinAgriculturalUniversityChangchun130118P.R.China MembraneChannelResearchLaboratory NortheastNormalUniversityChangchun130024P.R.China facultyoflifesciences LiaoningNormalUniversityDalian116029P.R.China

The glycine-to-aspartic acid missense mutation at the codon 551(G551D) of the cystic fibrosis transmembrane conductance regulator(CFTR) is one of the five most frequent cystic fibrosis(CF) mutations associated with a severe CF phenotype. To explore the feasibility of pharmacological correction of disrupted activation of CFTR chloride channel caused by G551D mutation, we developed a halide-sensitive fluorescence miniassay for G551D-CFTR in Fisher rat thyroid(FRT) epithelial cells for the discovery of novel activators of G551D-CFTR. A class of bicyclooctane small molecule compounds that efficiently stimulate G551D-CFTR chloride channel activity was identified by high throughput screening via the FRT cell-based assay. This class of compounds selectively activates G551D-CFTR with a high affinity, whereas little effect of the compounds on wildtype CFTR can be seen. The discovery of a class of bicyclooctane G551D-CFTR activators will permit the analysis of structure-activity relationship of the compounds to identify ideal leads for in vivo therapeutic studies.

关键词： Cystic fibrosis G551D-CFTR Activators Cell-based assay Small molecule High throughput screening

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