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Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

Asian pacific Journal of Reproduction 2024年第3期13卷 126-132页

作者： Anisa-Annur S Wan-Hafizah WJ Nor-Ashikin MNK Muhammad-Zaki R faculty of pharmacy and health Sciences Royal College of Medicine PerakUniversiti Kuala Lumpur30450 IpohPerakMalaysia Maternofetal and Embryo Research Group Faculty of MedicineUniversiti Teknologi MARASelangor BranchSungai Buloh Campus47000 Sungai BulohSelangorMalaysia

Objective:To investigate the effects of coenzyme Q10(CoQ10)supplementation on post-vitrification embryo development and gross ***:Balb/c mouse embryos were cultured in potassium simplex optimised medium(KSOM)with varying CoQ10 concentrations[0(control),20,40,and 60μM].The most effective CoQ10 concentration(40μM)was selected for subsequent post-vitrification morphology *** were randomly divided into four groups:Group A(non-vitrified without CoQ10),Group B(non-vitrified with CoQ10),Group C(vitrified without CoQ10),and Group D(vitrified with CoQ10),followed by vitrification at the 8-cell *** rates and development until the blastocyst stage were evaluated through morphological examinations using ASEBIR's system,distinguishing normal and abnormal ***:Supplementation of 40μM CoQ10 significantly increased blastocyst formation(95%)compared to the control group(92%),20μM(62%),and 60μM(56%)(P<0.001).Following vitrification,Group D exhibited a significant increase in blastocyst formation(92%)compared to Group C(82%)(P<0.05).Morphological assessments indicated superior embryo quality in Group B over Group D during the cleavage stage,morula,and blastocyst(P<0.05).Conclusions:CoQ10 supplementation exhibits promising potential to enhance preimplantation embryo development,increase blastocyst formation rates,and improve embryo quality *** offers a promising approach to mitigate oxidative stress on embryos,potentially improving overall assisted reproductive technology outcomes.

关键词： Coenzyme Q10 Antioxidant Embryo development Vitrification Morphology Assisted reproductive technology

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Quran memorisation and heart rate variability:How do they correlate?

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World Journal of Clinical Cases 2024年第29期12卷 6275-6284页

作者： Ahmad Rohi Ghazali Nor Malia Abd Warif Noor Anisah A Yazit Norsham Juliana Ismarulyusda Ishak Farah Wahida Ibrahim Arimi Fitri Mat Ludin Dzalani Harun Santibuana Abd Rahman Wan Nor Atikah Che Wan Mohd Rozali Biomedical Science Programme Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia Center for Toxicology and health Risk Studies Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia faculty of medicine and health Sciences Universiti Sains Islam MalaysiaNilai 71800Malaysia Center for healthy Ageing and Wellness Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia Center for Rehabilitation and Special Needs Studies Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia Occupational Therapy Programme Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia faculty of pharmacy and health Sciences Universiti Kuala Lumpur Royal College of Medicine PerakIpoh Perak 30450Malaysia

BACKGROUND Heart rate variability(HRV)was shown to be affected by performing religious *** To examine the relationship between the level of Quran(the holy book of Muslims)memorisation and HRV among *** This experimental study included 16 Tahfiz students and 16 non-Tahfiz students(n=32).The HRV was measured in three tasks:Recalling familiar verses,memorising new verses,and recalling the newly memorised verses of the *** analysis was done using these parameters:Standard deviation of N-N(heartbeat peak)interval;low frequency(LF);high frequency(HF)and LF/HF *** There were significant differences between tasks for all parameters(P<0.05).However,between the groups,only the LF/HF ratio had significant differences,with F=5.04,P<*** correlation showed a moderate positive correlation between the number of pages memorised and the LF/HF ratio(r=0.61,P<0.05).CONCLUSION Quran memorisation increased the HRV and our results suggested that this activity could be developed as an effective sympathovagal modulation training activity.

关键词： Heart rate variability Autonomic nervous system Quran memorisation Tahfiz student Heartbeat

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Biochemical synthesis of silver nanoprticles using filamentous fungi Penicillium decumbens (MTCC-2494) and its efficacy against A-549 lung cancer cell line

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Chinese Journal of Natural medicines 2016年第8期14卷 615-620页

作者： Shahnaz Majeed Mohd Syafiq bin Abdullah Gouri Kumar Dash Mohammed Tahir Ansari Anima Nanda faculty of pharmacy and health universiti kuala lumpur royal college of medicine ipoh perak malaysia 30450 faculty of Bioscience and chemical Engineering Sathyabama University Rajiv Gandhi Salai Chennai 600119 India

Biosynthesis of silver and other metallic nanoparticles is one of the emerging research area in the field of science and technology due to their potentiality, especially in the field of nano-biotechnology and biomedical sciences in order to develop nanomedicine. In our present study, Penicillium decumbens(MTCC-2494) was brought from Institute of Microbial Technology(IMTECH) Chandigarh and employed for extracellular biological synthesis of silver nanoparticles. Ag-NPs formation was appeared with a dark brown color inside the conical flask. Characterization of Ag-NPs were done by UV-Spectrophotometric analysis which showed absorption peak at 430 nm determines the presence of nanoparticles, Fourier transform infrared(FT-IR) spectroscopic analysis, showed amines and amides are the possible proteins involved in the stabilization of nanoparticles as capping agent. Atomic force Microscopy(AFM) confirmed the particle are spherical, size was around 30 to 60 nm and also the roughness of nanoparticles. Field emission scanning electron microscopy(FE-SEM) showed the topology of the nanoparticles and were spherical in shape. The biosynthesis process was found fast, ecofriendly and cost effective. Nano-silver particle was found to have a broad antimicrobial activity and also it showed good enhancement of antimicrobial activity of Carbenicillin, Piperacillin, Cefixime, Amoxicillin, Ofloxacin and Sparfloxacin in a synergistic mode. These Ag-NPs showed good anti-cancer activity at 80 μg·m L^(-1) upon 24 hours of incubation and toxicity increases upon 48 hours of incubation against A-549 human lung cancer cell line and the synergistic formulation of the antibiotic with the synthesized nanoparticles was found more effective against the pathogenic bacteria studied.

关键词： Silver nanoparticles Penicillium decumbens(MTCC-2494) Antibacterial Anti-cancer

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Identification of α-glucosidase inhibitors from Clinacanthus nutans leaf extract using liquid chromatography-mass spectrometry-based metabolomics and protein-ligand interaction with molecular docking

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Journal of Pharmaceutical Analysis 2019年第2期9卷 91-99页

作者： Suganya Murugesu Zalikha Ibrahim Qamar Uddin Ahmed Bisha Fathamah Uzir Nik Idris Nik Yusoff Vikneswari Perumal Faridah Abas Khozirah Shaari Alfi Khatib Department of Pharmaceutical Chemistry Kulliyyah of PharmacyInternational Islamic University Malaysia Department of Pharmaceutical Technology Faculty Pharmacy & Health SciencesUniversiti Kuala LumpurRoyal College of Medicine Perak Laboratory of Natural Products Institute of BioscienceUniversiti Putra Malaysia

The present study used in vitro and in silico techniques, as well as the metabolomics approach to characterise α-glucosidase inhibitors from different fractions of Clinacanthus nutans. C. nutans is a medicinal plant belonging to the Acanthaceae family, and is traditionally used to treat diabetes in malaysia. nHexane, n-hexane: ethyl acetate(1:1, v/v), ethyl acetate, ethyl acetate: methanol(1:1, v/v), and methanol fractions were obtained via partitioning of the 80% methanolic crude extract. The in vitro α-glucosidase inhibitory activity was analyzed using all the fractions collected, followed by profiling of the metabolites using liquid chromatography combined with mass spectrometry. The partial least square(PLS) statistical model was developed using the SIMCA P^+14.0 software and the following four inhibitors were obtained:(1) 4,6,8-Megastigmatrien-3-one;(2) N-Isobutyl-2-nonen-6,8-diynamide;(3) 1′,2′-bis(acetyloxy)-3′,4′-didehydro-2′-hydro-β, ψ-carotene; and(4) 22-acetate-3-hydroxy-21-(6-methyl-2,4-octadienoate)-olean-12-en-28-oic acid. The in silico study performed via molecular docking with the crystal structure of yeast isomaltase(PDB code: 3 A4 A) involved a hydrogen bond and some hydrophobic interactions between the inhibitors and protein. The residues that interacted include ASN259, HID295, LYS156, ARG335,and GLY209 with a hydrogen bond, while TRP15, TYR158, VAL232, HIE280, ALA292, PRO312, LEU313,VAL313, PHE314, ARG315, TYR316, VAL319, and TRP343 with other forms of bonding.

关键词： Clinacanthus nutans LC-MS-QTOF Metabolomics α-Glucosidase inhibitors Diabetes Molecular docking

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New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus

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Asian Journal of Pharmaceutical Sciences 2022年第3期17卷 435-446页

作者： Abdin Shakirin Mohamad Norpi Muhammad Luqman Nordin Nuraziemah Ahmad Haliza Katas Abdullah Al-Hadi Ahmad Fuaad Asif Sukri Nirmal Marasini Fazren Azmi Centre for Drug Delivery Technology Faculty of PharmacyUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia faculty pharmacy and health Sciences Universiti Kuala LumpurRoyal College of Medicine PerakPerak 30450Malaysia faculty of Veterinary medicine Universiti Malaysia KelantanKelantan 16100Malaysia Department of Chemistry Faculty of ScienceUniversiti MalayaKuala Lumpur 50603Malaysia universiti Teknologi Mara(UiTM) Bandar Puncak AlamIntegrative Pharmacogenomics Institute(iPROMISE)Selangor 43200Malaysia School of Biomedical Sciences The University of QueenslandSt.Lucia QLD 4072Australia

An effective vaccine against group A streptococcus(GAS)is highly desirable for definitive control of GAS *** the present study,two variants of amphiphilic chitosan nanoparticles-based GAS vaccines were *** vaccines were primarily composed of encapsulated KLH protein(a source of T helper cell epitopes)and lipidated M-protein derived B cell peptide epitope(lipoJ14)within the amphiphilic structure of *** only difference between themwas one of the nanoparticles vaccines received additional surface coating with poly(I:C).The formulated vaccines exhibited nanosized particles within the range of 220–240 *** uptake study showed that nanoparticles vaccine without additional poly(I:C)coating has greater uptake by dendritic cells and macrophages compared to nanoparticles vaccine that was functionalized with poly(I:C).Both vaccines were found to be safe in mice and showed negligible cytotoxicity against HEK293 *** immunization in mice,both nanoparticle vaccines produced high antigen-specific antibodies titres that were regulated by a balanced Th1 and Th2 response compared to physical *** antibodies elicited high opsonic activity against the tested GAS ***,our data demonstrated that amphiphilic chitosan nanoparticles platform induced a potent immune response even without additional inclusion of poly(I:C).

关键词： Amphiphilic chitosan nanoparticles Peptide vaccine Lipidation Multi-adjuvanting delivery system Immunogenicity Group A streptococcus

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Surface-engineered liposomes for dual-drug delivery targeting strategy against methicillin-resistant Staphylococcus aureus(MRSA)

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Asian Journal of Pharmaceutical Sciences 2022年第1期17卷 102-119页

作者： Nur Najihah Izzati Mat Rani Xiang Yi Chen Zahraa M.Al-Zubaidi Hanisah Azhari Tzar Mohd Nizam Khaitir Pei Yuen Ng Fhataheya Buang Geok Chin Tan Yin Ping Wong Mazlina Mohd Said Adeel Masood Butt Azmy A.Hamid Mohd Cairul Iqbal Mohd Amin Centre for Drug Delivery Technology Faculty of PharmacyUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia faculty of pharmacy and health Sciences University Kuala Lumpur Royal College of Medicine Perak No.3Perak 30450Malaysia Department of Medical Microbiology&Immunology Faculty of MedicineUniversiti Kebangsaan MalaysiaKuala Lumpur 56000Malaysia Drug and Herbal Research Center Faculty of PharmacyUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia Reading School of pharmacy University of ReadingReading RG66ADUnited Kingdom Department of Pathology Faculty of MedicineUniversiti Kebangsaan MalaysiaKuala Lumpur 56000Malaysia Institute of Pharmaceutical Sciences University of Veterinary and Animal SciencesLahore 54000Pakistan XORIX Sdn Bhd Dungun Terengganu 23000Malaysia

This study focused on the encapsulation of vancomycin(VAN) into liposomes coated with a red blood cell membrane with a targeting ligand, daptomycin–polyethylene glycol–1,2-distearoyl-sn-glycero-3-phosphoethanolamine, formed by conjugation of DAPT and Nhydroxysuccinimidyl-polyethylene glycol-1,*** formulation is capable of providing controlled and targeted drug delivery to the bacterial cytoplasm. We performed MALDI-TOF, NMR and FTIR analyses to confirm the conjugation of the targeting ligand via the formation of amide bonds. Approximately 45% of VAN could be loaded into the aqueous cores, whereas 90% DAPT was detected using UV–vis spectrophotometry. In comparison to free drugs, the formulations controlled the release of drugs for > 72 h. Additionally, as demonstrated using CLSM and flow cytometry, the resulting formulation was capable of evading detection by macrophage cells. In comparison to free drugs, red blood cell membrane–DAPT–VAN liposomes, DAPT liposomes, and VAN liposomes reduced the MIC and significantly increased bacterial permeability, resulting in > 80% bacterial death within 4 h. Cytotoxicity tests were performed in vitro and in vivo on mammalian cells,in addition to hemolytic activity tests in human erythrocytes, wherein drugs loaded into the liposomes and RBCDVL exhibited low toxicity. Thus, the findings of this study provide insight about a dual antibiotic targeting strategy that utilizes liposomes and red blood cell membranes to deliver targeted drugs against MRSA.

关键词： Erythrocyte Dual drug delivery Liposome Methicillin-resistant staphylococcus aureus Vancomycin Daptomycin

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Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats

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Asian Pacific Journal of Tropical Biomedicine 2023年第10期13卷 421-430页

作者： Rajeswari Ravindran Malathi Manuel Thangarajeswari Mohan Ravindran Jaganathan Kalaiselvi Periandavan Preclinical Department Faculty of MedicineUniversiti Kuala LumpurRoyal College of Medicine Perak30450IpohMalaysia Department of Medical Biochemistry Dr A.L Mudaliar Institute of Basic Medical SciencesUniversity of MadrasTaramaniChennai 600113India

Objective:To explore the combinatorial effect of epigallocatechin-3-gallate(EGCG)and calorie restriction on activation of nuclear factor erythroid 2-related factor 2(Nrf2),a transcription factor involved in the antioxidant defense system of aged ***:Aged male Wistar rats were calorie-restricted and treated with EGCG orally for 45 *** initial body weight of aged rats was recorded,and the final body weight was measured at the end of the experimental *** lipid and lipoprotein status,oxidative stress markers such as free radicals and malondialdehyde levels,and reduced glutathione were *** addition,RT-PCR and Western blotting analyses were ***:Calorie restriction potentiated the effect of EGCG on enhancing antioxidant status,improving the levels of serum lipid and lipoproteins,upregulating Nrf2 and Bcl2,and downregulating Keap1,cullin3,Bax and cytochrome c in aged ***:Calorie restriction can promote EGCG-mediated Nrf2 activation in aged *** preliminary finding paves the way for a combinatory approach to replenishing the antioxidant status during aging,thereby reducing the risk for age-associated degenerative diseases.

关键词： Aging Reactive oxygen species Calorie restriction EGCG Nrf2 Antioxidant

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COVID-19-associated mucormycosis and treatments

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Asian Pacific Journal of Tropical medicine 2021年第9期14卷 401-409页

作者： Vetriselvan Subramaniyan Shivkanya Fuloria Hari Kumar Darnal Dhanalekshmi Unnikrishnan Meenakshi Mahendran Sekar Rusli Bin Nordin Srikumar Chakravarthi Kathiresan V.Sathasivam Shah Alam Khan Yuan Seng Wu Usha Kumari Kalvatala Sudhakar Rishabha Malviya Vipin Kumar Sharma Neeraj Kumar Fuloria faculty of medicine Bioscience and NursingMAHSA UniversityJalan SP 2Bandar Saujana Putra42610 Jenjarom SelangorMalaysia faculty of pharmacy&Centre of Excellence for Biomaterials Engineering AIMST UniversityBedong 08100KedahMalaysia Jeffrey Cheah School of medicine&health Sciences Monash UniversitySelangor 47500Malaysia college of pharmacy National University of Science and Technology130 MuscatSultanate of Oman Department of Pharmaceutical Chemistry Faculty of Pharmacy and Health SciencesUniversiti Kuala Lumpur Royal College of Medicine PerakIpoh 30450Malaysia faculty of Applied Science&Centre of Excellence for Biomaterials Engineering AIMST UniversityKedahBedong 08100Malaysia Centre for Virus and Vaccine Research&Department of Biological Sciences School of Medical and Life SciencesSunway UniversitySelangor 47500Malaysia faculty of medicine AIMST UniversityKedah 08100Malaysia School of Pharmaceutical Sciences(LIT-pharmacy) Lovely Professional UniversityJalandhar 144411India Department of pharmacy SMASGalgotias UniversityGreater Noida 203201India Department of Pharmaceutical Sciences Gurukul KangriHaridwarUttarakhand 249404India

In the current pandemic,COVID-19 patients with predisposing factors are at an increased risk of mucormycosis,an uncommon angioinvasive infection that is caused by fungi with Mucor genus which is mainly found in plants and *** development in COVID-19 patient is related to various factors,such as diabetes,immunocompromise and *** use of glucocorticoids for the treatment of critically ill COVID-19 patients also leads to opportunistic infections,such as pulmonary ***-19 patients with mucormycosis have a very high mortality *** review describes the pathogenesis and various treatment approaches for mucormycosis in COVID-19 patients,including medicinal plants,conventional therapies,adjunct and combination therapies.

关键词： Mucormycosis COVID-19 Immunosuppression Pathogenesis Treatment

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Leveraging immunoliposomes as nanocarriers against SARS-CoV-2 and its emerging variants

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Asian Journal of Pharmaceutical Sciences 2023年第6期18卷 32-45页

作者： Nur Dini Fatini Mohammad Faizal Nurul Afina Ramli Nur Najihah Izzati Mat Rani Nur Adania Shaibie Aartid Pattaporn Poonsawas Sunil K.Sharma Mohd Cairul Iqbal Mohd Amin Centre for Drug Delivery Technology and Vaccine(CENTRIC) Faculty of PharmacyUniversiti Kebangsaan MalaysiaKuala Lumpur 50300Malaysia faculty of pharmacy University Royal College of Medicine Perak(UniKL RCMP)No.3Jalan GreentownIpoh 30450PerakMalaysia faculty of pharmacy Thammasat UniversityBangkok 10200Thailand Department of Chemistry University of DelhiDelhi 110007India

The global COVID-19 pandemic arising from SARS-CoV-2 has impacted many lives,gaining interest worldwide ever since it was first identified in December *** 2023,752 million cumulative cases and 6.8 million deaths were documented ***-19 has been rapidly evolving,affecting virus transmissibility and properties and contributing to increased disease *** Omicron is themost circulating variant of *** success in its treatment has indicated progress in tackling the virus,limitations in delivering the current antiviral agents in battling emerging variants remain *** the latest advancements in nanotechnology for controlling infectious diseases,liposomes have the potential to counteract SARS-CoV-2 because of their ability to employ different targeting strategies,incorporating monoclonal antibodies for the active and passive targeting of infected *** review will present a concise summary of the possible strategies for utilizing immunoliposomes to improve current treatment against the occurrence of SARSCoV-2 and its variants.

关键词： Coronavirus COVID-19 SARS-CoV-2 Liposomes Immunoliposomes

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Perceptions of malaysian medical students from different academic years on primary care:a qualitative research

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Family medicine and Community health 2020年第1期8卷 1-7页

作者： Sook Ching Chan Jaya Vinoshairine Ganeson Jee Tat Ong Sandheep Sugathan Community Based Department Faculty of MedicineUniversiti Kuala Lumpur Royal College of Medicine PerakIpohPerakMalaysia Hospital Sultanah Bahiyah Alor SetarKedah Darul AmanMalaysia Hospital kuala Pilah Kuala PilahNegeri SembilanMalaysia Department of Community medicine Quest International University PerakIpohPerakMalaysia

Objective To explore the perception of medical students from a private medical college in perak,malaysia,on primary care practice and induce the factors influencing their perception and willingness to consider primary care as a career *** Qualitative study using focus group ***’responses were audio recorded,transcribed,grouped under various domains and listed out and *** A private medical college in perak state,*** Forty-six medical students from years 2 to 5 were *** focus groups were formed with two focus groups from each academic year(six students each in seven groups and four students in one group).Students were informed through their respective student leader of each year and received a participant information sheet and an informed consent form which were completed and returned if they decided to participate in the focus group *** The participants had different levels of understanding of primary care depending on their level of exposure to primary *** students with more exposure had a better understanding about primary care and its *** factors towards choosing primary care as a career included short working hours with a more balanced family and social life,being able to treat patients as a whole with continuity of care and closer relationship with *** factors included routine,unchallenging and boring practice,poor salary,work overload and administrative work in government clinics,being less recognised by other specialties;and the poor perception by other doctors that those pursuing primary care were not‘brilliant enough’for more‘sophisticated disciplines like surgery or paediatrics’.Conclusion This study showed that the medical students’level of exposure to primary care played a crucial role in determining their understanding of primary care practice and their choice of career in primary *** to be addressed include remunera

关键词： discussions qualitative enough

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