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Inter-chromosomal insertions into wild-type chromosomes induced by SCRaMbLE

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Frontiers of Chemical Science and Engineering 2024年第9期18卷 125-135页

作者： Sijie Zhou Junyanrui Li Xichen Cui Ying Wang Ying-Jin Yuan Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Frontiers Research Institute for Synthetic Biology Tianjin UniversityTianjin 300072China

Genomic rearrangements play a crucial role in shaping biological phenotypic diversity and driving species *** chromosome rearrangement and modification by LoxP-mediated evolution(SCRaMbLE)has been applied to explore large-scale genomic rearrangements,yet it has been observed that these rearrangements occur exclusively in genomic regions containing loxPsym ***,we found that SCRaMbLE of synthetic yeast harboring synthetic chromosome V and X can generate a variety of synthetic segment insertions into wild-type chromosomes,ranging from 1 to 300 ***,it was revealed that the novel insertions impacted the transcriptional level of neighboring regions and affected the production of exemplar pathway of ***,our results improve the understanding of the ability of SCRaMbLE to generate complex structural variations in nonsynthetic regions and provide a potential model to explore genomic transposable events.

关键词： genomic rearrangements synthetic yeast genome SCRaMbLE inter-chromosomal insertions synthetic biology

Editable semiconductor photo-electrodes for sustainable ammonia synthesis

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Nano Research 2024年第4期17卷 3107-3112页

作者： Ning Lan Kun Du Erling Zhao Wei Zhao Tao Ling Key Laboratory for Advanced Ceramics and Machining Technology of Ministry of Education Institute of New-EnergySchool of Materials Science and EngineeringTianjin UniversityTianjin 300072China School of Materials Science and Engineering Tianjin Chengjian UniversityTianjin 300384China

Powered by an inexhaustible supply of solar energy,photoelectrochemical(PEC)nitrogen reduction reaction(NRR)provides an ideal solution for the synthesis of green ammonia(NH_(3)).Although great efforts have been made in the past decades,there are still significant challenges in increasing the NH_(3) yields of the PEC-NRR *** addition to the issues of low activity and selectivity similar to electrochemical NRR,the progress of PEC-NRR is also impeded by the limited increase in NH_(3) yields as the electrode is ***,we propose an editable electrode design strategy that parallels unit photo-electrodes to achieve a linear increase in NH_(3) yields with electrode active *** demonstrate that the editable electrode design strategy minimizes the electrode charge transfer resistance,allowing more photo-generated carriers to reach the electrode surface and promote the catalytic *** believe that this editable electrode design strategy provides an avenue to achieve sustainable PEC NH_(3) production.

关键词： editable electrode photoelectrochemical nitrogen reduction reaction parallel electrode ZnSe

Hydrogel-supported poly(L-lactic acid) and polystyrene microsphere-based three-dimensional culture systems for in vitro cell expansion

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Frontiers of Materials Science 2024年第2期18卷 129-137页

作者： Huaying Hao Lihong Sun Jiaxuan Chen Jun Liang State Key Laboratory of Food Nutrition and Safety Tianjin University of Science&TechnologyTianjin 300457China College of Light Industry Science and Engineering Tianjin University of Science&TechnologyTianjin 300457China College of Food Science and Engineering Tianjin University of Science&TechnologyTianjin 300457China

The in vitro expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ *** objective of this paper was to achieve static cell culture in vitro through peptide hydrogel-supported microspheres(MSs).The peptides,with their gel-forming properties,microstructures,and mechanical strengths characterized,were found to have good support for the MSs and to be *** internal structures of poly(L-lactic acid)microspheres(PLLA-MSs)and polystyrene microspheres(PS-MSs)made in thelaboratory were observed and statistically analyzed in terms of particle size and pore size,following which the co-cultured MSs with cells were found to have good cell *** addition,three-dimensional(3D)culturing of cells was performed on the peptide and microcarrier composite scaffolds to measure cell viability and cell *** results showed that the peptides could be stimulated by the culture medium to self-assembly form a 3D fiber network *** the peptide-Ms composite scaffold-based cell culture system,further enhancement of the cell culture effect was *** peptide-Ms composite scaffolds have great potential for the application in 3D cell culture and in vitro cellexpansion.

关键词： microcarrier microsphere peptide hydrogel cell scaffold three-dimensional culture cell expansion

Clinical outcome and prognostic factors of T4N0M0 colon cancer after R0 resection:A retrospective study

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 1869-1877页

作者： Bang Liu Zhao-Xiong Zhang Xin-Yang Nie Wei-Lin Sun Yong-Jia Yan Wei-Hua Fu Department of General Surgery Tianjin Medical University General HospitalTianjin Medical UniversityTianjin 300052China

BACKGROUND Paradoxically,patients with T4N0M0(stage II,no lymph node metastasis)colon cancer have a worse prognosis than those with T2N1-2M0(stage III).However,no previous report has addressed this *** To screen prognostic risk factors for T4N0M0 colon cancer and construct a prognostic nomogram model for these *** Two hundred patients with T4N0M0 colon cancer were treated at tianjin Medical university General Hospital between January 2017 and December 2021,of which 112 patients were assigned to the training cohort,and the remaining 88 patients were assigned to the validation *** between the training and validation groups were *** training cohort was subjected to multi-variate analysis to select prognostic risk factors for T4N0M0 colon cancer,followed by the construction of a nomogram *** The 3-year overall survival(OS)rates were 86.2%and 74.4%for the training and validation cohorts,***(P=0.000),T stage(P=0.001),right hemicolon(P=0.025),irregular review(P=0.040),and carbohydrate antigen 199(CA199)(P=0.011)were independent risk factors of OS in patients with T4N0M0 colon cancer.A nomogram model with good concordance and accuracy was *** Enterostomy,T stage,right hemicolon,irregular review,and CA199 were independent risk factors for OS in patients with T4N0M0 colon *** nomogram model exhibited good agreement and accuracy.

关键词： T4N0M0 colon cancer Prognosis Multivariate analysis Nomogram Colon cancer

Nanoscale“precision strike”:Tumor microenvironment-responsive smart micelles for efficient targeted drug delivery

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Nano Research 2024年第9期17卷 8360-8367页

作者： Dong Wan Yarong Song Xiujuan Lu Yanfeng Huang Jianxin Zhang Yonghui Liu Yi Liu Jie Pan School of Chemistry Tiangong UniversityTianjin 300387China School of Chemical Engineering and Technology Tiangong UniversityTianjin 300387China

To address the limitations of conventional nanotechnology-based drug delivery systems,this work developed enzyme and reduction dual-responsive polymeric *** micelles were synthesized with copolymers composed of TPGS_(3350)-PVGLIG-DOX(TPD)and FA-SS-DOX(FSD),which endow them with tumor-targeted drug delivery ***_(3350)contributes to extending the circulation of micelles in body,augmenting their accumulation in tumor tissues via the enhanced permeability and retention(EPR)*** localized the tumor site,matrix metalloproteinase 2(MMP2)cleaves the PVGLIG peptide moiety within the micelles,thereby releasing TPGS_(3350)and exposing the targeting ligand of *** approach enables the subsequent internalization of the micelles by tumor cells through folate receptor-mediated *** internalization,the high intracellular concentration of glutathione(GSH)triggers the reduction of the disulfide bond within the FA-SS-DOX,leading to the release of the anticancer-drug doxorubicin(DOX),which promotes apoptosis in the tumor cells and enhances the efficacy of chemotherapy.

关键词： cancer peptide micelle dual-responsive folate

Global dust density in two-dimensional complex plasma

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Chinese Physics B 2024年第6期33卷 445-450页

作者：赵逸真刘松芬孔伟杨芳 School of Physics Nankai UniversityTianjin 300071China College of Science Civil Aviation University of ChinaTianjin 300300China

The driven-dissipative Langevin dynamics simulation is used to produce a two-dimensional(2D) dense cloud, which is composed of charged dust particles trapped in a quadratic potential. A 2D mesh grid is built to analyze the center-to-wall dust density. It is found that the local dust density in the outer region relative to that of the inner region is more nonuniform,being consistent with the feature of quadratic potential. The dependences of the global dust density on equilibrium temperature, particle size, confinement strength, and confinement shape are investigated. It is found that the particle size, the confinement strength, and the confinement shape strongly affect the global dust density, while the equilibrium temperature plays a minor effect on it. In the direction where there is a stronger confinement, the dust density gradient is bigger.

关键词： dust particles quadratic potential two-dimensional mesh grid

Management of triplet excitons transition:fine regulation of Förster and dexter energy transfer simultaneously

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Light(Science & Applications) 2024年第2期13卷 302-315页

作者： Jiaqiang Wang Yujie Yang Xinnan Sun Xiaoning Li Liyao Zhang Zhen Li Institute of Molecular Aggregation Science Tianjin UniversityTianjin300072China School of Life Sciences Tianjin UniversityTianjin300072China Hubei Key Lab on Organic and Polymeric Opto-Electronic Materials Department of ChemistryWuhan UniversityWuhan430072China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan430072China Joint School of National university of Singapore Tianjin UniversityInternational Campus of Tianjin UniversityBinhai New CityFuzhou350207China

Understanding and management of triplet excitons transition in the same molecule remain a great ***,for the first time,by host engineering,manageable transitions of triplet excitons in a naphthalimide derivative NDOH were achieved,and monitored through the intensity ratio(ITADF/IRTP)between thermally activated delayed fluorescence(TADF)and room-temperature phosphorescence(RTP).Energy differences between lowest triplet excited states of host and guest were changed from 0.03 to 0.17 eV,and ITADF/IRTP of NDOH decreased by 200 times,thus red shifting the afterglow *** was proposed that shorter conjugation length led to larger band gaps of host materials,thus contributing to efficient Dexter and inefficient Förster energy ***,no transition to singlet state and only strongest RTP with quantum yield of 13.9%could be observed,when PBNC with loosest stacking and largest band gap acted as *** work provides novel insight for the management and prediction of triplet exciton transitions and the development of smart afterglow materials.

关键词： materials transition transitions

A preliminary study on life history of marine red alga Stylonema alsidii(Zandardini)Drew *** Zheng et Li

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Journal of Oceanology and Limnology 2024年第3期42卷 915-922页

作者： Bingxin HUANG Yao ZHANG Yue CHU Youxuan GUO Junxia LIANG Jianan QIN Lanping DING tianjin Key Laboratory of Animal and Plant Resistance College of Life SciencesTianjin Normal UniversityTianjin 300387China

Stylonema alsidii(Zandardini)Drew *** Zheng et Li is a common species in the genus Stylonema,but there are limited reports on morphological taxonomy and a lack of studies on life history and the regulation of early *** early development,discoid formation,and erect thallus growth of Stylonema alsidii *** were observed under laboratory *** addition,the influence of main environmental factors such as temperature and light intensity on its development was briefly studied,and various stages of its life history were *** result shows:(1)there are two reproductive modes:asexual reproduction and sexual reproduction;(2)the morphology of this species varies in different degrees under laboratory culture conditions;(3)sexual reproduction and asexual reproduction can coexist under suitable *** pathways of life history were inferred from experimental *** sporophytes form mature gametophytes by producing initially isolated or initially attached monospore,and mature gametophytes form mature sporophytes by fertilization of eggs and *** sexual and asexual reproduction processes of Stylonema alsidii *** were reported for the first time,and this is also the first report on sexual reproduction in the genus Stylonema.

关键词： Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li life history environmental factor asexual reproduction sexual reproduction

High adsorption selectivity of activated carbon and carbon molecular sieve boosting CO_(2)/N_(2) and CH_(4)/N_(2) separation

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Chinese Journal of Chemical Engineering 2024年第3期67卷 282-297页

作者： Siang Chen Wenling Wu Zhaoyang Niu Deqi Kong Wenbin Li Zhongli Tang Donghui Zhang The Research Center of Chemical Engineering School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Flue gas and coal bed methane are two important sources of greenhouse *** swing adsorption process has a wide range of application in the field of gas separation,and the selection of adsorbent is *** this regard,in order to assess the better adsorbent for separating CO_(2) from flue gas and CH_(4) from coal bed methane,adsorption isotherms of CO_(2),CH_(4) and N_(2) on activated carbon and carbon molecular sieve are measured at 303.15,318.15 and 333.15 K,and up to 250 *** experimental data fit better with Langmuir 2 compared to Langmuir 3 and Langmuir-Freundlich models,and Clausius-Clapeyron equation was used to calculate the isosteric *** the order of the adsorbed amount and the adsorption heat on the two adsorbents are CO_(2)>CH_(4)>N_(2).The adsorption kinetics are calculated by the pseudo-first kinetic model,and the order of adsorption rates on activated carbon is N_(2)-CH_(4)>CO_(2),while on carbon molecular sieve,it is CO_(2)-N_(2)>CH_(4).It is shown that relative molecular mass and adsorption heat are the primary effect on kinetics for activated carbon,while kinetic diameter is the main resistance factor for carbon molecular ***,the adsorption selectivity of CH_(4)/N_(2) and CO_(2)/N_(2) were estimated with the ideal adsorption solution theory,and carbon molecular sieve performed best at 318.15 K for both CO_(2) and CH_(4) *** study suggested that activated carbon is a better choice for separating flue gas and carbon molecular sieve can be a strong candidate for separating coal bed methane.

关键词： Activated carbon Carbon molecular sieve Adsorbent evaluation Adsorption equilibrium and kinetics Heat of adsorption Selectivity