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Photobiomodulation:a novel approach to promote trans-differentiation of adipose-derived stem cells into neuronal-like cells

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Neural Regeneration Research 2025年第2期20卷 598-608页

作者： Daniella Da Silva Madeleen Jansen van Rensburg Anine Crous Heidi Abrahamse Laser Research Centre Faculty of Health SciencesUniversity of JohannesburgDoornfonteinJohannesburgSouth Africa

Photobiomodulation,originally used red and near-infrared lasers,can alter cellular *** has been demonstrated that the visible spectrum at 451-540 nm does not necessarily increase cell proliferation,near-infrared light promotes adipose stem cell proliferation and affects adipose stem cell migration,which is necessary for the cells homing to the site of *** this in vitro study,we explored the potential of adipose-derived stem cells to differentiate into neurons for future translational regenerative treatments in neurodegenerative disorders and brain *** investigated the effects of various biological and chemical inducers on trans-differentiation and evaluated the impact of photobiomodulation using 825 nm near-infrared and 525 nm green laser light at 5 J/*** adipose-derived stem cells can be used in autologous grafting and photobiomodulation has been shown to have biostimulatory *** findings reveal that adipose-derived stem cells can indeed trans-differentiate into neuronal cells when exposed to inducers,with pre-induced cells exhibiting higher rates of proliferation and trans-differentiation compared with the control ***,green laser light stimulation led to notable morphological changes indicative of enhanced trans-differentiation,while near-infrared photobiomodulation notably increased the expression of neuronal *** biochemical analysis and enzyme-linked immunosorbent assays,we observed marked improvements in viability,proliferation,membrane permeability,and mitochondrial membrane potential,as well as increased protein levels of neuron-specific enolase and ciliary neurotrophic ***,our results demonstrate the efficacy of photobiomodulation in enhancing the trans-differentiation ability of adipose-derived stem cells,offering promising prospects for their use in regenerative medicine for neurodegenerative disorders and brain injuries.

关键词： differentiation inducers green photobiomodulation immortalized adipose-derived stem cell near-infrared photobiomodulation neurodegenerative disease neurogenesis photobiomodulation trans-differentiation

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Greenhouse gases fluxes and soil thermal properties in a pasture in central Missouri

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Journal of environmental sciences 2010年第7期22卷 1029-1039页

作者： Nsalambi Vakanda Nkongolo Shane Johnson Kent Schmidt Frieda Eivazi Department of agriculture and environmental sciences College of Agriculture and Natural Science Lincoln University Harvard Forest Faculty of Arts and Sciences Harvard University

Fluctuations of greenhouse gases emissions and soil properties occur at short spatial and temporal scales, however, results are often reported for larger scales studies. We monitored CO2, CH4, and N2O fluxes and soil temperature （T）, thermal conductivity （K）, resistivity （R） and thermal diffusivity （D） from 2004 to 2006 in a pasture. Soil air samples for determination of CO2, CH4 and N2O concentrations were collected from static and vented chambers and analyzed within two hours of collection with a gas chromatograph. T, K, R and D were measured in-situ using a KD2 probe. Soil samples were also taken for measurements of soil chemical and physical properties. The pasture acted as a sink in 2004, a source in 2005 and again a sink of CH4 in 2006. CO2 and CH4 were highest, but N2O as well as T, K and D were lowest in 2004. Only K was correlated with C02 in 2004 while T correlated with both N2O （r = 0.76, p = 0.0001） and CO2 （r = 0.88, p = 0.0001） in 2005. In 2006, all gases fluxes were significantly correlated with T, K and R when the data for the entire year were considered. However, an in-depth examination of the data revealed the existence of month-to-month shifts, lack of correlation and differing spatial structures. These results stress the need for further studies on the relationship between soil properties and gases fluxes. K and R offer a promise as potential controlling factors for greenhouse gases fluxes in this pasture.

关键词： greenhouse gases soil thermal properties fluxes

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Impact of Tillage and Herbicides on Weed Density, Yield and Quality of Cotton in Wheat Based Cropping System

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Journal of Integrative agriculture 2013年第9期12卷 1568-1579页

作者： Khalid Usman Niamatullah Khan Muhammad Umar Khan Aziz ur Rehman Said Ghulam Department of Agronomy Faculty of AgricultureGomal University Department of agriculture Chemistry Faculty of AgricultureGomal University Department of Soil & environmental sciences Faculty of AgricultureGomal University

Conservation tillage may improve yield of cotton in addition to improvement in soil quality if practiced for longer period. However, the practice may not be productive in short-term particularly when severe weeds are infesting the crops such as Cynodon dactylon, Conyza canadensis, Tribulus terrestris, and Cyperus rotundus, etc. Recent studies indicate that conventional tillage （CT） is more productive than zero tillage （ZT）/reduced tillage （RT）. Performance of cotton under three tillage systems, viz., ZT, RT and CT; and five herbicides, i.e., haloxyfop-R-methyl 10.8 EC （108 g a.i. ha^-1）, lactofen 24 EC （168 g a.i. ha^-1）, haloxyfop 10.8 EC ＋ lactofen 24 EC, hand weeding, and weedy check were evaluated during 2010-2011 at Gomal University, ***, Pakistan, to explore the best management option for effective weed control, enhanced yield and quality of cotton grown after wheat. The results revealed that hand weeding and Haloxyfop as post emergence alone or in combination with Lactofen reduced weed density to the minimum irrespective of the tillage systems. Excessive rainfall and cooler temperature limited cotton growth and yield in 2010. The adverse weather conditions had more adverse effect on boll weight under ZT and RT than CT. Haloxyfop ＋ lactofen produced higher seed cotton yield in RT than ZT, however, it could not exceed CT. Broad-spectrum herbicides x CT produced the highest number of bolls/plant, boll weight and seed cotton yield. Fiber quality and net returns were also the highest in broad-spectrum herbicides x CT. In conclusion, broad-spectrum herbicides under CT were more productive in wheat based cropping system on silty clay soil of ***.

关键词： tillage herbicide cotton seed cotton yield weed density net return

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A nucleotide substitution at the 5′ splice site of intron 1 of rice HEADING DATE 1(HD1) gene homolog in foxtail millet, broadly found in landraces from Europe and Asia

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The Crop Journal 2015年第6期3卷 481-488页

作者： Kenji Fukunaga Naoko Izuka Takehiro Hachiken Satoshi Mizuguchi Hidemi Ito Katsuyuki Ichitani faculty of Life and environmental sciences Prefectural University of Hiroshima faculty of agriculture Kagoshima University

We investigated genetic variation of a rice HEADING DATE 1(HD1) homolog in foxtail ***, we searched for a rice HD1 homolog in a foxtail millet genome sequence and designed primers to amplify the entire coding sequence of the gene. We compared full HD1 gene sequences of 11 accessions(including Yugu 1, a Chinese cultivar used for genome sequencing) from various regions in Europe and Asia, found a nucleotide substitution at a putative splice site of intron 1, and designated the accessions with the nucleotide substitution as carrying a splicing variant. We verified by RT-PCR that this single nucleotide substitution causes aberrant splicing of intron 1. We investigated the geographical distribution of the splicing variant in 480 accessions of foxtail millet from various regions of Europe and Asia and part of Africa by d CAPS and found that the splicing variant is broadly distributed in Europe and Asia. Differences of heading times between accessions with wild type allele of the HD1 gene and those with the splicing variant allele were unclear. We also investigated variation in 13 accessions of ssp. viridis, the wild ancestor, and the results suggested that the wild type is predominant in the wild ancestor.

关键词： Foxtail millet Geographical distribution HD1(HEADING DATE 1) homolog Setaria italica Splice site

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Integrated Weed Management Through Tillage and Herbicides for Wheat Production in Rice-Wheat Cropping System in Northwestern Pakistan

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Journal of Integrative agriculture 2012年第6期11卷 946-953页

作者： Khalid Usman Inayat Ullah Said Mir Khan Muhammad Umar Khan Said Ghulam Muhammad Anwar Khan Department of Agronomy Faculty of AgricultureGomal University Department of Entomology Faculty of AgricultureGomal University Department of agriculture Chemistry Faculty of AgricultureGomal University Department of Soil and environmental sciences Faculty of AgricultureGomal University

Conservational tillage （CT） in combination with broad spectrum herbicide may be more efficient and economical in controlling weeds, reducing cost of cultivation, and enhancing wheat yield. Field experiments were carried out to evaluate the effect of tillage and herbicides on weeds and wheat yield under rice-wheat cropping system （RWCS）. The results indicated that broad spectrum herbicides particularly, carfentrazone ethyl ester＋ isoproturon （Affinity 50 WDG） reduced weeds density by 93 and 95% compared to control and also produced the maximum grain yield （6 818 and 6 996 kg ha-l） and HI （harvest index, 44.7 and 44.6%） in 2007-2008 and 2008-2009 growing seasons, respectively. The maximum weeds infestation and the lowest yield were recorded in control. Tillage methods significantly influenced grain yield and HI, while no significant effect on all other parameters. The overall mean yield was significantly higher in zero tillage （5 575 kg ha-l） and reduced tillage （5 584 kg ha-l） compared to CT （5 479 kg ha-^-1）. Besides increasing wheat yield, Affinity 50 WDG and zero tillage are important weed management practices that can be integrated into wheat production in RWCS. However, in case of perennial weeds or insect pests＇ infestation, CT in combination with Affinity 50 WDG would be more productive than conservation tillage.

关键词： herbicides tillage weed population wheat yield

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Translational challenges in amyotrophic lateral sclerosis therapy with macrophage migration inhibitory factor

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Neural Regeneration Research 2025年第9期20卷 2583-2584页

作者： Leenor Alfahel Aleksandar Rajkovic Adrian Israelson Department of Physiology and Cell Biology Faculty of Health SciencesBen-Gurion University of the NegevBeer ShevaIsraelThe School of Brain Sciences and CognitionBen-Gurion University of the NegevBeer ShevaIsrael

Macrophage migration inhibitory factor(MIF):MIF acts as a pleiotropic inflammatory mediator,playing regulatory roles in innate and adaptive immunity,neuroinflammation,neuroendocrine functions,and nervous system development(Matejuk et al.,2024).In recent years,MIF has attra cted significant inte rest from research groups as a potential target for the treatment of various neurodegenerative diseases,including Alzheimer's disease,Parkinson's disease,multiple sclerosis,and glioblastoma(Matejuk et al.,2024).

关键词： immunity sclerosis

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Strength and compressibility behaviors of expansive soil treated with coffee husk ash

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Journal of Rock Mechanics and Geotechnical Engineering 2019年第2期11卷 337-348页

作者： M.K.Atahu F.Saathoff A.Gebissa Geotechnical Engineering and Coastal Engineering Faculty of Agriculture and Environmental SciencesUniversity of Rostock，Germany

Various factors can affect the durability of roads, such as the strength of sub-grade, the quality of the subbase and base course, the environments and properties of the soil used. Particularly, roads built on expansive soil are susceptible to early damage due to the swelling and shrinkage characteristics of this kind of soil under changing moisture conditions. The most common technique used to improve the properties of problematic soil is stabilization with additives. Using waste materials to improve the properties of expansive soil is a recent trend in soil stabilization. This study deals with the treatment of expansive soil with coffee husk ash(CHA). Coffee husk is a by-product of coffee production, and CHA is the resulting ash after burning it. In this study, the bearing capacity and compressibility characteristics of expansive soil(specifically black cotton(BC) soil) stabilized with varying percentages of CHA(5%, 10%,15%, and 20%) are investigated. Then, scanning electron microscopy(SEM) and energy dispersive X-ray(EDX) were used to analyze the influence of CHA on surface morphology and chemical composition of the studied soil. The results showed that the soil treated with CHA is generally improved in terms of *** of 20% CHA increases the bearing capacity of the soil by three-fold. In addition, the morphological studies of the soil samples treated with 10% and 15% CHA indicated the formation of hydrated particles and cementitious compounds as a result of the reaction between the soil and CHA. This indicates the potential usage of CHA as a stabilization agent and subsequently, it can address the disposal and environmental concerns related to coffee husk.

关键词： Black cotton (BC) soil Coffee husk Stabilization California bearing ratio (CBR) Consolidation Scanning electron microscopy (SEM)

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Machine Learning Techniques in Predicting Hot Deformation Behavior of Metallic Materials

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Computer Modeling in Engineering & sciences 2025年第1期142卷 713-732页

作者： Petr Opela Josef Walek Jaromír Kopecek Department of Metallurgical Technologies Faculty of Materials Science and TechnologyVSB–Technical University of OstravaOstrava70800Czech Republic FZU—Institute of Physics of the Czech Academy of sciences Prague18200Czech Republic

In engineering practice,it is often necessary to determine functional relationships between dependent and independent *** relationships can be highly nonlinear,and classical regression approaches cannot always provide sufficiently reliable ***,Machine Learning(ML)techniques,which offer advanced regression tools to address complicated engineering issues,have been developed and widely *** study investigates the selected ML techniques to evaluate their suitability for application in the hot deformation behavior of metallic *** ML-based regression methods of Artificial Neural Networks(ANNs),Support Vector Machine(SVM),Decision Tree Regression(DTR),and Gaussian Process Regression(GPR)are applied to mathematically describe hot flow stress curve datasets acquired experimentally for a medium-carbon *** the GPR method has not been used for such a regression task before,the results showed that its performance is the most favorable and practically unrivaled;neither the ANN method nor the other studied ML techniques provide such precise results of the solved regression analysis.

关键词： Machine learning Gaussian process regression artificial neural networks support vector machine hot deformation behavior

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Brain age estimation:premise,promise,and problems

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Neural Regeneration Research 2025年第8期20卷 2313-2314页

作者： Jarrad Perron Ji Hyun Ko Graduate Program in Biomedical Engineering Price Faculty of EngineeringUniversity of ManitobaWinnipegMBCanada PrairieNeuro Research Centre Kleysen Institute for Advanced MedicineHealth Sciences CentreWinnipegMBCanada Department of Human Anatomy and Cell Science Rady Faculty of Health SciencesUniversity of ManitobaWinnipegMBCanada

Premise:The com bined effects of modern healthcare practices which prolong lifespan and declining birthrates have created unprecedented changes in age demographics worldwide that are especially pronounced in Japan,South Korea,Europe,and North *** old age is the most significant predictor of dementia,global healthcare systems must rise to the challenge of providing care for those with neurodegenerative disorders.

关键词： estimation providing birth

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Vocal behavior of Crested Guineafowl(Guttera edouardi) based on visual and sound playback surveys in the Umhlanga Lagoon Nature Reserve,Kwa Zulu-Natal province,South Africa

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Chinese Birds 2015年第3期6卷 132-139页

作者： Johann H.van Niekerk Department of environmental sciences College of Agriculture and Environmental SciencesUniversity of South Africa

Background:Generally speaking,playbacks were often ineffective to determine the group sizes of birds since mainly males from leks *** limitation has not been tested properly for flocking birds such as Crested Guineafowl(Guttera edouardi) with the view to use it as a counting *** aims of the study reported in this paper were(1) to describe the cal s of Crested Guineafowl in a social context;(2) to interpret cal s in an evolutionary context;and(3) to demonstrate that playbacks can be used to locate and count Crested Guineafowl in smal isolated ***:The vocal behavior of Crested Guineafowl was observed during a survey conducted in the Umhlanga Lagoon Nature Reserve(Kwa Zulu-Natal,South Africa) from November 2010 to June *** line observations and sound playback methods were ***:The behavioral context and structure of cal s were *** eight cal s described for Crested Guineafowl were produced mainly to unite flock members in the dense understory,where visibility was poor,and to repel *** not disturbed,Crested Guineafowl were quiet and only made soft contact ***:The high rate of call back and the fact that flocks invariably approach the source of the call en masse,shows that field researchers are able to assess the population size of Crested Guineafowl with sound playbacks along a *** value of playbacks as a conservation tool is assessed.

关键词： Guttera edouardi Playback Calls Social behavior Flocking Transect surveys

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