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Neural Regeneration Research 2024年第3期19卷 542-547页

作者： María Norte-Muñoz David García-Bernal Diego García-Ayuso Manuel Vidal-Sanz Marta Agudo-Barriuso Grupo de Investigación Oftalmología experimental Departamento de OftalmologíaOptometríaOtorrinolaringología y Anatomía PatológicaFacultad de MedicinaUniversidad de MurciaInstituto Murciano de Investigación Biosanitaria(IMIB)Campus de Ciencias de la Salud30120 MurciaSpain Grupo de Investigación Trasplante Hematopoyético y terapia celular Departamento de Bioquímica e Inmunología.Facultad de MedicinaUniversidad de MurciaInstituto Murciano de Investigación Biosanitaria(IMIB)Campus de Ciencias de la Salud30120 MurciaSpain

Advanced mesenchymal stromal cell-based therapies for neurodegenerative diseases are widely investigated in preclinical models.Mesenchymal stromal cells are well positioned as therapeutics because they address the underlying mechanisms of neurodegeneration,namely trophic factor deprivation and neuroinflammation.Most studies have focused on the beneficial effects of mesenchymal stromal cell transplantation on neuronal survival or functional improvement.However,little attention has been paid to the interaction between mesenchymal stromal cells and the host immune system due to the immunomodulatory properties of mesenchymal stromal cells and the long-held belief of the immunoprivileged status of the central nervous system.Here,we review the crosstalk between mesenchymal stromal cells and the immune system in general and in the context of the central nervous system,focusing on recent work in the retina and the importance of the type of transplantation.

关键词： adaptive immunity cell therapy central nervous system immune system innate immunity mesenchymal stromal cells neuroregeneration preclinical studies retina transplantation

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Novel insights into the pathogenesis of tendon injury:mechanotransduction and neuroplasticity

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Neural Regeneration Research 2022年第10期17卷 2223-2224页

作者： Suellen Alessandra Soares de Moraes faculdade de fisioterapia e terapia ocupacional Universidade Federal do ParáBelémParáBrazil

Tendon pathology is characterized by damage to the tendon structural integrity with disruption of collagen fibers(Nourissat et al.,2015).Acute tendon injuries show a macroscopic discontinuity,ranging from partial to complete tendon rupture.They involve inflammation and lead to loss of motion.In chronic conditions(or tendinopathy),symptoms include changes in both locomotor and sensorial functions of the tendon(Nourissat et al.,2015;Scott et al.,2020).Inconsistency in terminology for cases of painful tendon disorders is found,but recently the term tendinopathy was established in consensus as preferable for cases with persistent tendon pain and loss of function related to mechanical loading.This term excludes a problem in clinical practice-i.e.,specification of the presence of a particular pathological or biochemical process(Scott et al.,2020).

关键词： tendon injuries Acute

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Transcranial direct current electrical stimulation in combination with aerobic exercise:A pilot study in post-COVID-19 systemic autoimmune rheumatic patients

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World Journal of Rheumatology 2023年第1期11卷 1-12页

作者： Rafael Giovani Missé Alexandre Moura dos Santos Isabela Bruna Pires Borges Marlise Sítima Mendes Simões Lorenza Rosa Silvério Bruna Lindoso Correia Ana Wook Sook Kim Aline Marques Caetano Sandra Gofinet Pasoto Carla Gonçalves Schahin Saad Diogo Souza Domiciano Clarice Tanaka Julia Maria D’Andrea Greve Abrahão Fontes Baptista Samuel Katsuyuki Shinjo Division of Rheumatology Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil departamento de fisioterapia Fonoaudiologia e Terapia OcupacionalHospital das Clínicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloNúcleo de Assistência e Pesquisa em NeuromodulaçãoSao PauloBrazil Laboratório de estudos do Movimento Instituto de Ortopedia e TraumatologiaHospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil

BACKGROUND Systemic autoimmune rheumatic diseases(SARDs)are a group of diseases with multiorgan involvement and a high prevalence of chronic pain and fatigue.Patients with SARDs and post-coronavirus disease 2019(COVID-19)syndrome experience aggravation of symptoms.In this context,it is essential to establish strategies to reduce chronic pain and fatigue and improve quality of life.AIM To assess the efficacy of transcranial direct current stimulation(tDCS)for the treatment of fatigue and pain-associated post-COVID-19 syndrome in patients with SARDs.MeTHODS This study included nine patients with different types of SARDs.All patients had reverse transcription-polymerase chain reaction(RT-PCR)test confirmed COVID-19 as well as significant,persistent fatigue and pain that began to worsen after infection.Anodal tDCS was administered in five daily sessions(2mA,20 min).Concomitantly,patients were involved in aerobic exercise program.All participants were evaluated using specific questionnaires and strength assessment by handgrip and physical function by timed-up-and-go test and sit-to-stand test at baseline(within one week before tDCS protocol),and one week after tDCS protocol.During all procedures,the patients’treatments remained unchanged.ReSULTS The sample comprised eight women and one man with a mean age of 48.7±9.6 years.After the tDCS protocol,pain and fatigue significantly improved on the visual analog scale(Pe physical function also improved 9.5±2.7 vs 6.8±0.8(P=0.001)for timed-up-go-test and 10.3±3.7 vs 15.1±4.0(P=0.037)for sit-to-stand test.None of the patients experienced any adverse events.CONCLUSION The present study showed that tDCS in combination with aerobic exercise was effective in improving physical function,and reducing fatigue/pain in SARDs patients with post-COVID-19 syndrome.

关键词： Autoimmune diseases COVID-19 Fatigue Neuromodulation Physical function Rheumatic diseases Pain

effect of aging on behaviour of mesenchymal stem cells

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World Journal of Stem Cells 2019年第6期11卷 337-346页

作者： Juan Antonio Fafián-Labora Miriam Morente-López María C Arufe Grupo de terapia Celular y Medicina Regenerativa Departamento de Fisioterapia Ciencias Biomédicas y MedicinaUniversidade da Coru?a

Organs whose source is the mesoderm lineage contain a subpopulation of stem cells that are able to differentiate among mesodermal derivatives (chondrocytes, osteocytes, adipocytes). This subpopulation of adult stem cells, called “mesenchymal stem cells” or “mesenchymal stromal cells (MSCs)”, contributes directly to the homeostatic maintenance of their organs;hence, their senescence could be very deleterious for human bodily functions. MSCs are easily isolated and amenable their expansion in vitro because of the research demanding to test them in many diverse clinical indications. All of these works are shown by the rapidly expanding literature that includes many in vivo animal models. We do not have an in-depth understanding of mechanisms that induce cellular senescence, and to further clarify the consequences of the senescence process in MSCs, some hints may be derived from the study of cellular behaviour in vivo and in vitro, autophagy, mitochondrial stress and exosomal activity. In this particular work, we decided to review these biological features in the literature on MSC senescence over the last three years.

关键词： Mesenchymal stem cells Aging Autophagy Mitochondrial stress extracellular vesicles

Major liver resections,perioperative issues and posthepatectomy liver failure:A comprehensive update for the anesthesiologist

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World Journal of Critical Care Medicine 2024年第2期13卷 49-71页

作者： Andrea de Gasperi Laura Petrò Ombretta Amici Ilenia Scaffidi Pietro Molinari Caterina Barbaglio eva Cibelli Beatrice Penzo elena Roselli Andrea Brunetti Maxim Neganov Alessandro Giacomoni Paolo Aseni elena Guffanti Former Head Anesthesia and Critical Care Service 2Grande Ospedale Metropolitano Niguarda ASST GOM NiguardaMilan 20163Italy AR1 Ospedale Papa Giovanni 23Bergamo 24100Italy Anesthesia and Critical Care Service 2 Grande Ospedale Metropolitano Niguarda AR2ASST GOM NiguardaMilan 20163Italy Anestesia e terapia Intensiva Generale Istituto Clinico HumanitasRozzano 20089Italy Chirurgia Oncologica Miniinvasiva Grande Ospedale Metropolitano Niguarda ASST GOM NiguardaMilan 20163Italy Dipartimento di Medicina d’Urgenza ed emergenza Grande Ospedale Metropolitano Niguarda ASST GOM NiguardaMilano 20163MIItaly

Significant advances in surgical techniques and relevant medium-and long-term outcomes over the past two decades have led to a substantial expansion in the indications for major liver resections.To support these outstanding results and to reduce perioperative complications,anesthesiologists must address and master key perioperative issues(preoperative assessment,proactive intraoperative anesthesia strategies,and implementation of the enhanced Recovery After Surgery approach).Intensive care unit monitoring immediately following liver surgery remains a subject of active and often unresolved debate.Among postoperative complications,posthepatectomy liver failure(PHLF)occurs in different grades of severity(A-C)and frequency(9%-30%),and it is the main cause of 90-d postoperative mortality.PHLF,recently redefined with pragmatic clinical criteria and perioperative scores,can be predicted,prevented,or anticipated.This review highlights:(1)The systemic consequences of surgical manipulations anesthesiologistsmust respond to or prevent,to positively impact PHLF(a proactive approach);and(2)the maximal intensivetreatment of PHLF,including artificial options,mainly based,so far,on Acute Liver Failure treatment(s),to buytime waiting for the recovery of the native liver or,when appropriate and in very selected cases,toward livertransplant.Such a clinical context requires a strong commitment to surgeons,anesthesiologists,and intensivists towork together,for a fruitful collaboration in a mandatory clinical continuum.

关键词： Liver resection Chronic liver disease Preoperative assessment Vascular clamping Intraoperative hemodynamic monitoring Postoperative intensive care unit Posthepatectomy liver failure Artificial liver support

Maternal hyperthyroidism increases the synthesis activity and the osteogenic markers expression of calvarial osteoblasts from offspring in a murine model

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BIOCeLL 2023年第2期47卷 423-430页

作者： FABIANA ROCHA ARAÚJO BRUNO MACHADO BeRTASSOLI ISABeLLA CRISTINA SOUZA FÉLIX DOUGLAS MARINHO ABReU NATÁLIA MeLO OCARINO AMANDA MARIA SeNA ReIS JUNeO FReITAS SILVA ROGÉRIA SeRAKIdeS Núcleo de Células Tronco e terapia Celular Animal(NCT-TCA) Veterinay SchoolUniversidade Federal de Minas GeraisBelo HorizonteMinas Gerais31270-901Brasil Universidade de Uberaba(UNIUBe) UberabaMinas Gerais38065-300Brasil Instituto de Ciências Biológicas Universidade Federal de Minas GeraisBelo HorizonteMinas Gerais31270-901Brasil Universidade estadual de Santa Cruz Campus Soane Nazare de AndradeIlheusBahiaBrasil

To evaluate the characteristics and synthesis activity of osteoblasts extracted from the calvaria of offspring of rats exposed to maternal hyperthyroidism.Twelve adult Wistar rats were divided into two groups,one control and one treated with daily administration of L-thyroxine by an orogastric tube(50μg/animal/day)during pregnancy.Three days after delivery and confirmation of the mothers’hyperthyroidism,the offspring were euthanized for the extraction of osteoblasts from the calvaria.At 7,14,and 21 days,proliferation activity was assessed using MTT assay,while alkaline phosphatase(ALP)activity was assessed by the BCIP/NBT method.At 21 days,the total area of the mineralized matrix stained by von Kossa was evaluated by morphometry.The expression of gene transcripts for Runx2,Bmp2,Fgfr1,collagen type 1(Col1),osteocalcin(Oc),and osteopontin(Op)were evaluated by real-time RT-PCR.Means were compared using the Student’s t-test.FA activity was significantly higher at 14 and 21 days in cultures of osteoblasts extracted from offspring exposed to maternal hyperthyroidism,while MTT conversion was significantly lower at 21 days in this group.Osteoblast cultures of neonates exposed to maternal hyperthyroidism also showed a larger total area of mineralized matrix and greater expression of gene transcripts for Oc and Op.Maternal hyperthyroidism increases the activity of matrix synthesis,alkaline phosphatase activity,and expression of gene transcripts for osteocalcin and osteopontin in the osteoblasts,extracted from the calvaria of the offspring,which may be one of the mechanisms of premature fusion of cranial sutures.

关键词： Craniosynostosis Intramembranous growth Ossification Rat Thyroid dysfunction

COVID-19 pandemic in the intensive care unit:Psychological implications and interventions,a systematic review

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World Journal of Psychiatry 2023年第4期13卷 191-217页

作者： Laura Monti elisa Marconi Maria Grazia Bocci Georgios demetrios Kotzalidis Marianna Mazza Carolina Galliani Sara Tranquilli Giovanni Vento Giorgio Conti Gabriele Sani Massimo Antonelli Daniela Pia Rosaria Chieffo UOS Psicologia Clinica Governo ClinicoFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC Anestesia RianimazioneTerapia Intensiva e Tossicologia ClinicaDipartimento di Scienze Dell’EmergenzaAnestesiologiche e Della RianimazioneFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC Psichiatria Clinica e d’Urgenza Dipartimento di Scienze Dell’InvecchiamentoNeurologicheOrtopediche e Della Testa-colloFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy NeSMOS department Facoltàdi Medicina e PsicologiaSant’Andrea University HospitalRome 00189Italy department of Psychiatry UniversitàCattolica del Sacro CuoreRome 00168Italy department of Psychology UniversitàCattolica del Sacro CuoreRome 00168Italy UOC Neonatologia Dipartimento di Scienze Della Salute Della DonnaDel Bambino e di SanitàPubblicaFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC Neonatologia UniversitàCattolica del Sacro CuoreRome 00168Italy UOC terapia Intensiva Pediatrica e Trauma Center Pediatrico Dipartimento di Scienze Dell’EmergenzaAnestesiologiche e Della RianimazioneFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC terapia Intensiva Pediatrica e Trauma Center Pediatrico UniversitàCattolica del Sacro CuoreRome 00168Italy department of Anesthesiology and Intensive Care Medicine UniversitàCattolica del Sacro CuoreRome 00168Italy

BACKGROUND The coronavirus disease 2019(COVID-19) pandemic produced changes in intensive care units(ICUs) in patient care and health organizations. The pandemic event increased patients’ risk of developing psychological symptoms during and after hospitalisation. These consequences also affected those family members who could not access the hospital. In addition, the initial lack of knowledge about the virus and its management, the climate of fear and uncertainty, the increased workload and the risk of becoming infected and being contagious, had a strong impact on healthcare staff and organizations. This highlighted the importance of interventions aimed at providing psychological support to ICUs, involving patients, their relatives, and the staff;this might involve the reorganisation of the daily routine and rearrangement of ICU staff duties.AIM To conduct a systematic review of psychological issues in ICUs during the COVID-19 pandemic involving patients, their relatives, and ICU staff.MeTHODS We investigated the PubMed and the ClinicalTrials.gov databases and found 65 eligible articles,upon which we commented.ReSULTS Our results point to increased perceived stress and psychological distress in staff, patients and their relatives and increased worry for being infected with severe acute respiratory syndrome coronavirus-2 in patients and relatives. Furthermore, promising results were obtained for some psychological programmes aiming at improving psychological measures in all ICU categories.CONCLUSION As the pandemic limited direct inter-individual interactions, the role of interventions using digital tools and virtual reality is becoming increasingly important. All considered, our results indicate an essential role for psychologists in ICUs.

关键词： COVID-19 Intensive care unit Psychological interventions Pandemic Mental health Health care professionals

Inter-Individual Rapid Force Improvements after Mixed Session and Traditional Periodization in Aging Adults:A Randomized Trial

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Journal of Science in Sport and exercise 2021年第2期3卷 125-137页

作者： Bruno Monteiro Moura ewertton de Souza Bezerra Lucas B.R.Orssatto Antonio Renato Pereira Moro Fernando Diefenthaeler Universidade Federal de Santa Catarina Centro de DesportosLaboratório de BiomecânicaFlorianópolisCEP 88040-900Brazil Universidade Federal do Amazonas Faculdade de Educação Física e FisioterapiaLaboratório de Estudo do Desempenho HumanoManausCEP 38400-678Brazil School of exercise and Nutrition Sciences Queensland University of TechnologyBrisbane 4059Australia

Background Different periodization models have been widely used to improve maximum and rapid force in aging adults.However,it is unclear if some specific model can influence the remarkable heterogeneity of responsiveness to rapid torque improvements following resistance training.Aims Compare the effects of mixed session periodization(MSP)and traditional(TP)models on neuromuscular performance and individual responsiveness in aging adults.Methods Twenty-two aging men and women(64.6±5.2 years old;1.68±0.1 m;77.8±16.0 kg)completed the intervention period.They were assigned into MSP(n=11)or TP(n=11)resistance training for lower limbs.The outcome measures normalized dynamic leg press(5-RM:BM),absolute and normalized knee isometric peak torque(PT and PT:BM)and rate of torque development,and impulse were assessed at baseline and after three blocks of a 9-wk resistance training(3 sessions per week).Thigh leg lean mass was assessed by dual-energy x-ray absorptiometry at baseline and after the intervention.Results Similar increases were observed for 5-RM:BM(MSP:28.8%±13.5%and TP:26.4%±7.6%,Perved for PT:BM(MSP:16.4%±16.5%and TP:4.7%±9.2%,P>0.05).Absolute and normalized RTD remained unchanged throughout intervention(P>0.05),but both groups improved impulse(MSP:22.1%±25.7%and TP:12.6%±45.2%,Pesponders(n=5)presented significant time effect for absolute RTD(Pe(Periodization protocols increased 5-RM:BM and PT,it was not interchangeable through improve-ments in RTD even adopting power training session.Furthermore,these results suggest a great inter-individual variability following different periodization models for aging people.

关键词： Rate of torque development Impulse Strength training Muscular strength Hypertrophy Periodization

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

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Cell Research 2006年第4期16卷 329-336页

作者： Patrizia Bossolasco Tiziana Montemurro Lidia Cova Stefano Zangrossi Cinzia Calzarossa Simona Buiatiotis Davide Soligo Silvano Bosari Vincenzo Silani Giorgio Lambertenghi deliliers PaoloRebulla LorenzaLazzari Fondazione Matarelli 20121 Milanltaly Cell Factory Centro di Medicina TrasfusionaleTerapia Cellulare e CriobiologiaFondazione IRCCS Ospedale Maggiore PoliclinicoMangiagalli e Regina Elena20122 Milan Italy Dipartimento Cell Factor Centro di Medicina TrasfusionaleTerapia Cellulare e CriobiologiaFondazione IRCCS Ospedale Maggiore PoliclinicoMangiagalli e Regina Elena20122 Milan Italy Dipartimento di Anatomia Patologica Ospedale San Paolo20142 MilanItaly ematologia

The main goal of the study was to identify a novel source of human multipotent cells,overcoming ethical issues in-volved in embryonic stem cell research and the limited availability of most adult stem cells.Amniotic fluid cells(AFCs)are routinely obtained for prenatal diagnosis and can be expanded in vitro;nevertheless current knowledge about theirorigin and properties is limited.Twenty samples of AFCs were exposed in culture to adipogenic,osteogenic,neurogenicand myogenic media.Differentiation was evaluated using immunocytochemistry,RT-PCR and Western blotting.Beforetreatments,AFCs showed heterogeneous morphologies.They were negative for MyoD,Myf-5,MRF4,Myogenin anddesmin but positive for osteocalcin,PPARgamma2,GAP43,NSe,Nestin,MAP2,GFAP and beta tubulin Ⅲ by RT-PCR.The cells expressed Oct-4,Rex-1 and Runx-1,which characterize the undifferentiated stem cell state.By immunocyto-chemistry they expressed neural-glial proteins,mesenchymal and epithelial markers.After culture,AFCs differentiatedinto adipocytes and osteoblasts when the predominant cellular component was fibroblastic.early and late neuronal an-tigens were still present after 2 week culture in neural specific media even if no neuronal morphologies were detectable.Our results provide evidence that human amniotic fluid contains progenitor cells with multi-lineage potential showingstem and tissue-specific gene/protein presence for several lineages.

关键词： amniotic fluid cells adult stem cells mesenchymal stem cells cellular differentiation plasticity

epigenetic marks are modulated by gender and time of the day in the hippocampi of adolescent rats:a preliminary study

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Neural Regeneration Research 2018年第12期13卷 2160-2163页

作者： Viviane Rostirola elsner Laura Reck Cechinel Louisiana Carolina Ferreira de Meireles Karine Bertoldi Ionara Rodrigues Siqueira Programa de Pós-Graduacao em Ciências Biológicas:Fisiologia Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do Sul Programa de Pós-Graduacao em Biociências e Reabilitacao Centro Universitário Metodista-IPA Curso de fisioterapia Centro Universitário Metodista-IPA

Although the involvement of gender in epigenetic machinery in peripheral tissues during the neonatal period has been suggested, the gender-related epigenetic profile of brain areas during the adolescent period is rarely exploited. Furthermore, the influence of time of day on hippocampal acetylation marks has been demonstrated in young adult and aged rats; however, there are no studies reporting epigenetic changes in the adolescent period. Therefore, this study aimed to investigate the effects of gender on hippocampal DNA methyltransferase 1 content and histone deacetylase（HDAC） activity of adolescent rats at different time points, specifically early morning and afternoon. Both epigenetic markers increased significantly in the hippocampi of female rats compared to the male group, an indicator of reduced transcriptional activity. In addition, HDAC activity during the early morning was higher compared to afternoon groups in both male and female rats, while DNA methyltransferase 1 content was not altered by the time of day. Our findings demonstrate that hippocampal DNA methylation and histone acetylation status can be influenced by gender during the adolescent period, while the time of the day impacts HDAC activity.

关键词： adolescent rats DNA methyltransferase 1 histone deacetylase hippocampus time of the day gender epigenetic marks

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