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Recombinant Helicoverpa armigera nucleopolyhedrovirus with arthropod-specific neurotoxin gene RjAa 17f from Rhopalurus junceus enhances the virulence against the host larvae

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Insect Science 2017年第3期24卷 397-408页

作者： Huan Yu Bin Zhou Jiao Meng Jian Xu Tong-Xian Liu Dun Wang State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&F UniversityYangling Shaanxi China Key Laboratory of Applied Entomology Northwest A&F UniversityYangling Shaanxi China

A recombinant Helicoverpa armigera nucleopolyhedrovirus （HearNPV） ex- pressing the insect-selective neurotoxin （RjAalTjO from Cuban scorpion Rhopalurus junceus was constructed by replacing the UDP-glucosyltransferase gene （egt） using ）^- red homologous recombination system. Another egt deleted control HearNPV was con- structed in a similar way by inserting egfp gene into the egt locus. One-step viral growth curve and viral DNA replication curve analysis confirmed that the recombination did not affect the viral growth and DNA replication in host cells. There is no discernable differ- ence in occlusion-body morphogenesis between RjAalTf-HearNPV,, Egfp-HearNPV and HZ8-HearNPV, which was confirmed by transmission electron microscopy analysis. How- ever, the insecticidal activity of RjAal 7f-HearNPV is enhanced against the third instar H. armigera larvae according to the bioassay on virulence comparison. There is a dramatic reduction （56.9%） in median lethal dose （LD50） and also a reduction （13.4%） in median sur- vival time （ST50） for the recombinant RjAalTf-HearNPV compared to the HZ8-HearNPV, but only a 27.5% reduction in LD50 and 10.1% reduction in ST50 value when Egfp- HearNPV is compared with HZ8-HearNPV. The daily diet consumption analysis showed that the RjAal 7f-HearNPV was able to inhibit the infected larvae feeding compared with the egt minus HearNPV. These results demonstrated that this novel recombinant RjAa17f- HearNPV could improve the insecticidal effect against its host insects and RjAa17fcould be a considerable candidate for other recombinant baculovirus constructions.

关键词： HearNPV recombinant baculovirus RjAal 7f, virulence improvement

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f?(H2O)+CH3I Ligand Exchange Reaction Dynamics

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Chinese Journal of Chemical Physics 2020年第2期33卷 210-216,I0003,I0039-I0040页

作者： Bjorn Bastian Tim Michaelsen Milan Oncák Jennifer Meyer Roland Wester Institut für Ionenphysik und Angewandte Physik Universitat InnsbruckTechnikerstraβe 256020 InnsbruckAustria

Single hydration of the gas phase f^-+CH3I→CH3f reaction allows to probe solvent effects on a fundamental nucleophilic substitution *** the same time,the addition of a solvent molecule opens alternative product ***,we present crossed beam imaging results on the dynamics of the f^-(H2O)+CH3I→[fCH3I]^-+H2O ligand exchange pathway at collision energies between 0.3 and 2.6 *** kinetic energies are constrained by the stability requirement of the weakly bound product *** implies substantial internal excitation of the water molecule and disfavors effcient energy redistribution in an intermediate complex,which is reflected by the suppression of low kinetic energies as collision energy *** 0.3 eV,internal nucleophilic displacement is important and is discussed in light of the competing nucleophilic substitution pathways that form I^- and I^-(H2O).

关键词： Reaction dynamics Ligand exchange Microsolvation Crossed beams Velocity map imaging

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Neuronspezifische　Enolase　（NSE）：　ein　Serummarker　der　klinischen　Progression　für　maligne　metastasierte　Melanome

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1995年第1期15卷 19-25页

作者：郭华北 Abteilung für Klinische Biochemie Bengbu Medizinische Institut Bengbu Institut für Klinische Biochemie der Universität Bonn Deutschland

Die Serumkonzentration der neuronspezifischen Enolase(NSE)wurde bei 126 Patienten mit malignem Melanom(MM,Stadium Ⅰ/Ⅱ:n=80;Stadium Ⅲ:n=23;Stadium Ⅳ:n=23)radioimmunologisch *** NSEKonzentrationen(Schwellenwert:>12,5μg/L)fanden sich bei 12,5% der Patienten mit MM im Stadium Ⅰ/Ⅱ,13,0% im Stadium Ⅲ und bei 39,1% im Stadium Ⅳ.In der Langzeit-Verlaufskontrolle bei 20 Patienten im Stadium Ⅲ/Ⅳ zeigte die NSESerumkonzentration eine Ubereinstimmung mit der *** 6 Patienten zeigte sich unter der Therapie ein Abfall erhohter NSE-Werte als Zeichen einer *** Ausbleiben eines signifikanten Abfalls oder gar ein Asstieg wies in 86,7%(13/15)der falle auf eine Tumorprogression *** signifikanter Anstieg von NSE wurde bei 6 Patienten prdfinal *** laβt sich folgern,daβ NSE fur die Primardiagnostik des malignen Melanoms aufgrund der niedrigen Sensitivitatat als Tumormarker ungeeignet ist;jedoch erscheint eine NSE-Bestimmung bei Patienten mit malignem Melanom im Rahmen einer Verlaufskontrolle sinnvoll.

关键词： Malignes Melanom NSE Serum-Tumormarker

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Identification of three Berberis species as potential alternate hosts for Puccinia striiformis f. sp. tritici in wheat-growing regions of Xinjiang, China

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Journal of Integrative Agriculture 2019年第12期18卷 2786-2792页

作者： ZHUANG Hua ZHAO Jing HUANG Li-li KANG Zhen-sheng ZHAO Jie State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University

Since the recent discovery of barberry(Berberis spp.)as an alternate host for the stripe rust pathogen Puccinia striiformis,many Chinese Berberis species have been identified as alternate hosts for *** ***,little is known about Berberis species and their distribution in wheat-growing regions in Xinjiang,China,where stripe rust is *** the largest province or autonomous region,Xinjiang represents a relatively independent epidemic region for wheat stripe rust in *** this study,we con ducted a survey of barberry plants in the main wheat-growi ng areas of *** iden tified three Berberis species,***,*** and ***,and con firmed their roles as potential alternate hosts for *** *** in the laboratory.

关键词： barberry alternate host sexual reproduction stripe rust yellow rust

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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of Puccinia striiformis f. sp. tritici

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frontiers of Agricultural Science and Engineering 2017年第1期4卷 48-58页

作者： Yuan TIAN Gangming ZHAN Xia LU Jie ZHAO Lili HUANG Zhensheng KANG State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University China-Australia Joint Research Centre for Abiotic and Biotic Stress Management Northwest A&F University

Wheat stripe rust caused by Puccinia striiformis f. sp. tritici is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/*** isolate of P. striiformis f. sp. tritici was selfed on Berberis shensiana to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing Yrgenes and genotyped with 96 simple sequencing repeat markers, with51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with Yr5,Yr10, Yr15, Yr24 and Yr26 and virulent on lines with Yr17,Yr25 and YrA, indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with Yr1,Yr2, Yr4, Yr6, Yr7, Yr8, Yr9, Yr27, Yr28, Yr32, Yr43, Yr44,YrExp2, YrSp, YrTr1, YrTye and YrV23. The 17 cultivars to which the Pst was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

关键词： Puccinia striiformis f. sp. tritici selfing heterozygosity virulence inheritance

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Comparing AdS/CfT dipole model to HERA f_2 data

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Chinese Physics C 2010年第9期34卷 1421-1424页

作者：吕准 Department of Physics Southeast University Departamento de física y Centro de Estudios Subatómicos Universidad Técnica Federico Santa María

We apply an AdS/CfT-inspired color-dipole model which contains only three free parameters to describe the HERA data for the inclusive structure function f 2 at small Bjorken-x and *** found that the saturation scale in our AdS/CfT-based parameterization varies in the range of 1 ÷ 3 GeV becoming independent of energy/Bjorken-x at very small *** leads to the prediction of x-independence of the structure functions at very small *** the fitted parameters in our model,the predictions for f 2,longitudinal structure function,charm structure function and total photo-production cross-sections in the kinematic regions of future experiments can be given.

关键词： color dipole model AdS/CfT correspondence structure function saturation scale

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Zircon and titanite geochronology of the f(u|¨)rstenstein granite massif,Bavarian forest,NW Bohemian Massif:Pulses of the late Variscan magmatic activity

Zircon and titanite geochronology of the F(u|¨)rstenstein g...

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中国科学院地质与地球物理研究所2004学术年会

作者： WOLfGANG SIEBEL Institut fr Geowissenschaften

The f(u|¨)rstenstein granite massif is the largest late Variscan granitoid body exposed in the Moldanubian basement of the Bavarian *** granite massif comprises different rock types,ranging from diorite to granite in *** study provides precise zircon and titanite ages,obtained by the U-Pb isotopic dilution and Pb-Pb evaporation methods,and Nd-Sr isotopic data on this *** dating constrains an intrusive sequence as following:Dioritic rocks crystallised about 334 to 332 Ma ago, earlier than the high-temperature peak stage of the Variscan metamorphism of the Moldanubian basement(325 to 320 Ma) in the Bavarian *** medium- to coarse-grained Tittling granite was emplaced about 10 Ma later,between 324 and 321 Ma,while the medium-grained Eberhardsreuth granite crystallised between 316 and 312 Ma,contemporaneously with the coarse-grained porphyritic Saldenburg *** from the dioritic rocks give U-Pb ages of about 321±4 Ma,indicating thermal resetting or crystallisation due to the intrusion of the Tittling *** diorites have initialε values of -1.3 to -2.9 and relatively low initial Sr/Sr ratios of 0.7056 to 0.7067,which suggest mantle contribution to melts or melting of young mafic lower *** granites have similar initialε values of -3.6 to -4.1 but show some variation in their Sr isotopic composition(initial Sr/Sr ratios of 0.7063 to 0.7074),probably indicating the involvement of crustal material in their genesis.

关键词： Bavarian forest fiirstenstein Variscan granite zircon age

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Second-order optimality conditions f or minimizing a maxfunction

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Science China Mathematics 2000年第7期43卷 722-733页

作者：黄力人 K.f.Ng Department of Mathematics South China Normal University Guangzhou 510631 China Department of Mathematics The Chinese University of Hong KongHong KongChina Correspondence should be addressed to K.F.Ng

We study Chaney's and Ben-Tal-Zowe's second-order directional derivatives with applications in minimization problem for max-functions of the formh(x): = max {f(x, τ); τ ∈T},where T is a compact metricspace. We impr... 详细信息

关键词： max-f unction sup-type function second-order optimality condition nonsmooth optimization.

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Application Status and Promotion Strategy of Integrated Network Teaching Platform——Taking Northwest A&f University as an Example

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Asian Agricultural Research 2019年第4期11卷 90-92页

作者： Na WANG Yinghui ZHANG College of Humanities and Social Development Northwest A&F University Academic Affairs Office Northwest A&F University

The development of Internet technology has brought convenience to the development of education and teaching,and has also changed the traditional methods and means of education and teaching. The construction of an integrated network teaching platform based on Internet technology brings new opportunities for the development of higher education. Taking the construction of the integrated network teaching platform of Northwest A&f University as an example,the current situation and existing problems in the construction of the integrated network teaching platform of the Northwest A&f University are explained. By proposing the promotion strategies for the application of the integrated network teaching platform,the construction of the integrated network teaching platform is improved,thereby facilitating the online and offline mixed teaching and further promoting the improvement of teaching level and teaching effect.

关键词： Integrated network teaching platform Mixed teaching Promotion strategies

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Hydrolysis mechanism of carbendazim hydrolase from the strain Microbacterium ***-6f

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Journal of Environmental Sciences 2017年第4期29卷 171-177页

作者： Ji Lei Shaopeng Wei Lijun Ren Shibin Hu Peng Chen Key Laboratory of Disaster Survey and Mechanism Simulation of Shaanxi Province College of Chemistry and Chemical EngineeringBaoji University of Arts and Sciences College of Resources and Environment College of Life Sciences and College of Plant ProtectionNorthwest A&F University

The carbendazim（MBC） hydrolyzing enzyme gene was cloned and heterologously expressed in Escherichia coli BL21（DE3） from a newly isolated MBC-degrading bacterium strain Microbacterium sp. strain djl-6f. High performance liquid chromatography-mass spectrometry（HPLC-MS）analysis revealed that purified MheI-6f protein catalyzes direct hydrolysis of MBC into2-aminobenzimidazole（2-AB） with a high turnover rate and moderate affinity（Kmof6.69 μmol/L and kcatof 160.88/min） without the need for any cofactors. The optimal catalytic condition of MheI-6f was identified as 45°C, pH 7.0. The enzymatic activity of MheI-6f was found to be diminished by metal ions, and strongly inhibited by sodium dodecyl sulfate（SDS）.Through generating amino acid mutations in MheI-6f, Cys16 and Cys222 were identified as the catalytic groups that are essential for the hydrolysis of MBC. This is the first report on the biodegradation of MBC at the enzymatice level.

关键词： Carbendazim Microbacterium sp.djl-6f Hydrolase MheI-6f

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