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Poor hygienic conditions of butcheries and high level of microbiological contamination of meat sold in Nampula city,Mozambique

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Food and Health 2023年第2期5卷 6-12页

作者： Ndjate Kinyamba-Junior Cátia Salamandane Vanito Frei Acacio Salamandane Pompílio Vintuar Faculdade de Ciências Agrarias Universidade LúrioCamus Universitário de Unango3300 Sanga-NiassaMozambique Laboratório de Qualidade e Segurança Alimental Centro de estudos Interdisciplinares Lúrio(CEIL)Universidade LúrioCampus Universitário de Marrere4250 NampulaMozambique Faculdade de Ciências de Saúde Universidade LúrioCampus Universitário de Marrere4250 NampulaMozambique Faculdade de Geografia Universidade RovumaCampus Universitário de Napipine5440 NampulaMoçambique Centro de Extensão Inovação e Transferência de Conhecimento(CEITRAC)Universidade RovumaCampus Universitário de Napipine5440 NampulaMoçambique Faculdade de Ciências Alimentares e Agrarias Universidade RovumaCampus Universitário de Napipine5440 NampulaMozambique

The present study aimed to evaluate the hygiene practices and the quality of meat sold in butcheries in the city of Nampula,*** the twelve butcheries in the city,only seven participated in the study,which was divided into four categories:hygienic conditions of buildings and facilities,equipment,furniture and utensils,good handling practices for handlers,and microbiological quality of meat *** showed that most of the butcheries(71%)had poor hygiene conditions,such as walls with cracks and holes and lack of washbasins in the production *** addition,the majority(71%)of butcheries used a reusable dirty cloth to clean equipment and utensils,and only 43%had a cold room or refrigerated chamber for meat *** personal hygiene,100%of the handlers washed and changed their clothes daily,but 71%did not properly wash their hands before handling *** analysis revealed that 86%of the cutting tables swabs and 43%of the hand blades had *** counts above 4 Log,and the presence of Salmonella was observed in 71%of meat ***,the study highlights the need for improvement in hygiene practices and the quality of meat sold in butcheries in Nampula.

关键词： Salmonella spp poor hygienic conditions butcheries facilities Nampula meat handling

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Editorial:Aquaculture genomics

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Aquaculture and Fisheries 2019年第1期4卷 1-2页

作者： Adelino V.M.Canário CCMAR-CIMAR Centre of Marine SciencesUniversity of AlgarveCampus de GambelasFaro 8005-139Portugal International Research Centre for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai 201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China

What can genomics offer to aquaculture and fisheries?The definition of Genomics,retrieval of biological meaning from large sets of DNA and/or RNA sequences present in organisms,highlights the potential benefits that can be accrued from this burgeoning *** continuous advances in sequencing technologies and bioinformatic tools,in particular in the last decade,enabled the harnessing of large amounts of genome-wide data from nonmodel organisms which can be used to address both fundamental and applied questions of fisheries,aquaculture and ecology(Abdelrahman et al.,2017).Genomics informed management is now considered absolutely essential if aquaculture and fisheries are to achieve their promise of making a significant contribution to sustainable food security,as the world demand for high quality protein continues to increase(Abdelrahman et al.,2017;Bernatchez et al.,2017;Yanez,Newman,&Houston,2015).However,although genomics approaches have become more common their practical use to improve fisheries management and aquaculture production still has not fulfilled its promise,despite overwhelming evidence of its advantages and benefits(Bernatchez et al.,2017;Garner et al.,2016;B.;Louro,Power,&Canario,2014).Among the possible solutions to stimulate wider application of genomics knowledge are the need for greater communication among fishery scientists,geneticists,fishery managers,and also better communication of the potential of genomics approaches and the results to stakeholders by adoption of a clear and understandable dialogue(Bernatchez et al.,2017).While this is still a stumbling block that needs to be addressed in multiple contexts,it is equally important that more genomics-based papers are published in journals of a multidisciplinary nature such as Aquaculture and *** special issue is a small but a significant step towards communicating the power of genomics to a wide potential user community.

关键词： absolutely benefits promise

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Which is the best choice for gastroesophageal disorders:Melatonin or proton pump inhibitors?

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2010年第5期1卷 102-106页

作者： Joanna Dulce Favacho de Oliveira Torres Ricardo de Souza Pereira Universidade Federal do Amapá Campus Universitário Marco Zero do Equador Rod. Juscelino Kubitschek KM-02 Jardim Marco Zero CEP 68.902-280 Macapá AP Brazil Laboratório de Laser e Microscopia de Fora Atmica Dr. Nivaldo Parizotto Departamento de Fi- sioterapia Universidade Federal de So Carlos Rod. Washington Luís So Carlos SP Brazil

Melatonin is used in many countries to improve sleep *** is a hormone produced by the pineal gland and enterochromaff in cells which control sleep and gastrointestinal *** levels of melatonin lead to gastroesophageal reflux disease(GERD).Most of patients with GERD have a sleep ***,low melatonin levels is the main cause of *** this,it has an inhibitory action on gastric acid secretion and seems to control the lower esophageal *** pump inhibitors(PPIs) are a group of drugs whose main action is a pronounced and long-lasting reduction of gastric acid *** are the most potent inhibitors of acid secretion available ***(one of the PPIs) and melatonin have similarities in their chemical ***,we could consider omeprazole as a rough copy of *** this paper,we compare the advantages and disadvantages of the clinical use of melatonin and PPIs.

关键词： Melatonin Sleep disorder Gastroesophageal reflux disease Omeprazole Ulcers Gastritis

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Effect of Glucose Concentrations on the Growth and Metabolism of Brettanomyces bruxellensis under Aerobic Conditions

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Advances in Microbiology 2013年第3期3卷 227-232页

作者： Ortiz-Muniz Benigno Corro-Herrera Victor Gomez-Rodriguez Javier Domínguez-González JoséManuel Aguilar-Uscanga María Guadalupe Instituto Tecnológico Superior de Tierra Blanca Av.Veracruz s/n.Col.PEMEXVeracruzMéxico Instituto Tecnológico de Veracruz Unidad de Investigación y Desarrollo en Alimentos(UNIDA)Dpto Ing.Eléctrica y ElectrónicaCol.Formando HogarVeracruzMéxico Universidad de Vigo Departamento de Ingeniería QuímicaFacultad de CienciasCampus OurenceOurenseSpain

Acetic acid can be directly produced from glucose in one-step fermentation by using yeasts of the genus Brettanomyces bruxellensis, hence increasing the industrial application to manufacture products with simplified bioprocesses. Thereby, this work evaluates the influence of initial glucose concentration on the growth and acetic acid production by B. bruxellensis. The results obtained confirmed the presence of Crabtree effect on B. bruxellensis under low glucose concentrations. The maximum acetic acid concentration reached was 15.4 g·L-1 starting with 100 g·L-1 leading to a product yield of 0.154 g·g-1 and a specific acetic acid production rate of 0.05 g·g-1·h-1. The results also indicate that after reaching the acetic acid critic threshold of 4 g·L-1 the metabolism can induce the growth second phase even residual glucose was present on the culture media at high starting glucose concentrations. Additionally, it was observed a lineal relationship between cell viability and acetic acid production.

关键词： Glucose Brettanomyces bruxellensis Acetic Acid Inhibition Crabtree Effect

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Valorisation of fruit waste for enhanced exopolysaccharide production by Xanthomonas campestries using statistical optimisation of medium and process

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Food Bioscience 2022年第2期46卷 1014-1019页

作者： Avni Vaishnav Kinjal Upadhyay Manoj Koradiya Devayani Tipre Shailesh Dave Department of Microbiology School of ScienceGujarat UniversityAhmedabad380009GujaratIndia Department of Biochemistry and Biotechnology St.Xavier’s CollegeAhmedabad380009GujaratIndia Department of Microbiology L.J.Institutes of Applied SciencesS.G.RoadAhmedabad382210GujaratIndia Xavier Research Foundation Loyola Centre for R&DSt.Xavier’s College CampusAhmedabad380009GujaratIndia

The aim of this research work was to enhance the fermentative production of exopolysaccharide(EPS)with mixed fruit waste as a key *** campestries was isolated from spoiled orange and its EPS production efficiency was determined using EPS *** to optimisation,the production was 3.4 g/L,upon optimizing the medium the production improved to 9.2 g/*** medium formulation was studied by sequential addition of medium components and replacement of sugar with mixed fruit *** formulated medium,the production enhanced to 11.1 g/*** further optimisation,the response surface methodology was used,which resulted in the enhancement of production to 14.5 g/*** scale-up study was undertaken in a 10 L shake flask and *** 10 L flask EPS,production was 14.45 g/L in 100 h,whereas in 10 L fermenter it was 21.10 g/L in 60h *** optimisation studies gave nearly 500%(6.2fold)enhanced EPS production and dwindle in fermentation *** of sucrose with fruit waste extract made the process environment-friendly;omission of L-cystine and use of 50%reduced amount of sodium acetate in the medium lowered the production cost of *** developed process resulted in the utilization of fruit waste and was also useful in solving the fruit waste disposal problem.

关键词： Exopolysaccharide Mixed fruits waste Formulated medium Scale up Response surface methodology

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Coefficient in Tomato Seedling Cultivated with Hydroretentor

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Journal of Agricultural Science and Technology(B) 2018年第8期8卷 507-511页

作者： Catariny Cabral Aleman Flávio Campos Bastos Agricultural Engineering Departament Federal University of Vicosa Ph Rolfs Avenue s/n Campus Universitário Vicosa-MG 36572-028 Brazil

The objective of this study was to obtain the weekly crop coefficient (Kc) for tomato seedlings in substratum with waterproofing polymer and irrigated by automatic system of localized irrigation. The experiment was conducted in an agricultural greenhouse located in the irrigation experimental area of the Department of Agricultural Engineering, Vi?osa-MG, Brazil. The crop coefficient (Kc) for tomato seedlings was obtained for the conditions of the experiment (in plastic trays of 128 cells, in greenhouse, in Vi?osa-MG, for the spring/summer season), using the substrates with and without hydrogel. The irrigation system was micro sprinkler, consisting of four emitters per tray of seedlings, so that each tray constitutes an individualized experimental unit. The method for estimation of reference evapotranspiration was Penman-Monteith FAO56. The weighing lysimetry was used to obtain the variation of the tray mass from the evapotranspiration of the culture. Two consecutive daily weights, spaced at intervals of 60 min (during the morning shift), were performed for five sample units (SU) arranged in a completely randomized design in the greenhouse. Each SU was composed of a tray of 128 cells. The values of Kc obtained were used to calibrate the crop evapotranspiration (ETc) estimates used in the later stages. Weekly Kc values were generated. Irrigation was managed so that the crop did not undergo water stress during the moulting phase. For the conditions tested it was possible to obtain Kc values between 0.72 and 2.86 for the substrate without hydrogel and 0.81 to 3.46 for the substrate with hydrogel. It should be considered that the production stage of seedlings is very demanding in water and in protected environment conditions it is possible to obtain high Kc values.

关键词： Water need water economy automatic irrigation.

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Single and Hybrid Design Polycaprolactone （PCL） Scaffolds： Cell Culture Study

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材料科学与工程（中英文A版） 2013年第5期3卷 315-320页

作者： Md Enamul Hoque Yong Leng Chuan Ian Pashby Siti Sheren Aini Angela Ng Min Hwei Ruszymah Idrus Department of Mechanical Materials and Manufacturing Engineering University of Nottingham Malaysia Campus Malaysia Tissue Engineering Centre Universiti Kebangsaan Malaysia Medical Centre Malaysia

Hybrid design strategy in scaffold development is currently being explored in order to obtain new scaffold geometries in favor of efficient cell attachment and growth in tissue engineering applications. This current study investigates the cell culture performance on the single and hybrid design PCL scaffolds manufactured via in-house built desktop robot based rapid prototyping (DRBRP) system. Single design scaffolds were developed by drawing fibers at a specific angle (e. g., 30°, 45°, 60° or 90°) throughout the scaffold unit. Unlikely, the hybrid design scaffolds integrated two or more lay-down patterns (e. g., 30-45-60-900) in the same scaffold unit. In vitro cell culture studies were performed on both single and hybrid design scaffolds by using human osteoprogenitor cells. Light, scanning electron and confocal laser microscopy (CLM) showed cell attachment, proliferation, and extracellular matrix production on the surface as well as inside the scaffold architecture. Hybrid design scaffolds demonstrated better performance in cell culture studies than the single design scaffolds. The overall results suggest that the hybrid scaffolds offer better optimized properties, which can meet the functional and biological requirements of living tissues for regenerative therapies.

关键词：体外细胞培养混合设计 PCL 支架聚己内酯激光共聚焦显微镜快速原型系统细胞外基质

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On-Surface Bottom-Up Construction of COF Nanoshells towards Photocatalytic H_(2)production

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Research 2021年第1期2021卷 923-934页

作者： Yao Chen Dong Yang Yuchen Gao Runlai Li Ke An Wenjing Wang Zhanfeng Zhao Xin Xin Hanjie Ren Zhongyi Jiang Key Laboratory for Green Chemical Technology School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin 300072China Key Laboratory of Systems Bioengineering of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China School of Environmental Science and Engineering Tianjin UniversityTianjin 300072China College of Polymer Science and Engineering Sichuan UniversityChengdu 610065China Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityBinhai New CityFuzhou 350207China

The rational design of an outer shell is of great significance to promote the photocatalytic efficiency of core-shell structured ***,a covalent organic framework(COF)nanoshell was designed and deposited on the cadmium sulfide(CdS)core surface.A typical COF material,TPPA,featuring exceptional stability,was synthesized through interfacial polymerization using 1,3,5-triformylphloroglucinol(TP)and p-phenylenediamine(PA)as *** nanoshell endows the CdS@TPPA nanosphere with ordered channels for unimpeded light-harvesting and fast diffusion of reactants/products and well-defined modular building blocks for spatially charge ***,the heterojunction formed between CdS and TPPA can further facilitate the effective charge separation at the interface via lower exciton binding energy compared with that of pristine *** modulating the thickness of TPPA nanoshell,the CdS@TPPA nanosphere photocatalyst with the nanoshell thickness of about 8±1 nm exhibits the highest photocatalytic H2 evolution of 194.1μmol h^(-1)(24.3 mmol g^(-1)h^(-1),8 mg),which is superior to most of the reported COF-based *** framework nanoshell in this work may stimulate the thinking about how to design advanced shell architecture in the core-shell structured photocatalysts to achieve coordinated charge and molecule transport.

关键词： shells exceptional rational

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The endophytic mycobiota of Arabidopsis thaliana

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Fungal Diversity 2013年第3期60卷 71-89页

作者： Elena García Ángela Alonso Gonzalo Platas Soledad Sacristán Centro de Biotecnología y Genómica de Plantas(UPM-INIA)and E.T.S.I.Agrónomos Universidad Politécnica de MadridCampus de MontegancedoAutopista M40(Km.38)28223 Pozuelo de Alarcón MadridSpain Departamento de Biotecnología E.T.S.I.AgrónomosUniversidad Politécnica de MadridAvda.Complutense s/n28040 MadridSpain Fundación Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía MEDINA Parque Tecnológico de las Ciencias de la Salud de GranadaAvda del Conocimiento 318100 ArmillaGranadaSpain Instituto de Recursos Naturales y Agrobiología Consejo Superior de Investigaciones CientíficasAvenida Reina Mercedes 1041012 SevillaSpain

Fungal endophytes are receiving increasing attention as resources to improve crop production and ecosystem ***,the biology and ecological significance of these symbionts remains poorly understood,due to a lack of model systems for more efficient *** this work,we have analyzed the culturable endophytic mycobiota associated,in the wild,with leaves and siliques of the model plant *** have studied the effect of biotic and abiotic factors in the frequency of fungal endophytes in plant specimens,and in the species composition of the endophytic *** results indicate that the frequency of Arabidopsis plants hosting endophytes depends on the time of the year and the phenological stage of the plant,and that the probability of endophyte colonization increases as the life cycle of the plant *** diversity of the endophytic assemblages of natural *** populations was high,and precipitation and temperature were the two main factors determining the diversity and species composition of the *** propose *** and its endophytes as a model system for an integral approach to the principles governing the endophytic lifestyle,taking advantage of the molecular tools and the abundant knowledge accessible from the host plant.

关键词： Endophyte Mycobiota Arabidopsis Wild populations Ecology

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Total Phenols,Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

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Phyton-International Journal of Experimental Botany 2019年第2期88卷 139-147页

作者： M.Y.Ovando-Domínguez M.C.Luján-Hidalgo D.González-Mendoza A.A.Vargas-Díaz N.Ruiz-Lau F.A.Gutiérrez-Miceli C.A.Lecona-Guzmán Laboratorio de Cultivo de Tejidos Vegetales.Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez Carretera Panamericana Km.1080.Tuxtla GutiérrezChiapasC.P.29050México Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC).Carretera a Delta s/n C.P.21705 Ejido Nuevo LeónBaja CaliforniaMéxico CONACYT-Colegio de Postgraduados Campus CampecheCarretera Federal Haltunchén-Edzna km 17.5.SihochacChampotónCampecheCP 24450México CONACYT-Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez.Carretera Panamericana Km.1080.Tuxtla Gutiérrez ChiapasC.P.03940México

Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog(MS)medium supplemented with different concentrations of 1-naphthylacetic acid(NAA),6-benzyladenine(BA)and 2,4-dichlorophenoxyacetic acid(2,4-D)utilized hypocotyls with *** highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for ***(100%)while for *** in lower percentage(75%).BA stimulated the formation of shoots in all the evaluated concentrations,being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for ***(23 shoots/explant)and ***(28 shoots/explant).The content of total phenols,flavonoids and antioxidant activity was measured in the callus obtained from both *** results showed that a higher content of total phenols was quantified in callus of ***(27.8 mg g-1 dw)compared to ***(23.2 mg g-1 dw).The highest content of total flavonoids was observed in the callus of ***(8.0μg g-1 dw).Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydracil radical *** concentration required for 50%inhibition(IC50)of the 2,2-diphenyl-1-picrylhydracil radicals were 4.22μg mL-1 in methanolic extracts of callus of ***,while in extracts of callus of *** was 2.86μg mL-1,in both cases was greater than that found for *** culture of the species studied in this work represents an alternative for the production of natural antioxidants.

关键词： Annonaceae antioxidant activity 6-benzyladenine secondary metabolites tissue culture

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